60th Anniversary of the Malta Historical Society : A Commemoration

The paper traces the survival of the archive of the Roman Inquisition in Malta (1561-1798) during the French republican occupation, between June 1798 and September 1800. The primary sources identified and utilised in the study enable a detailed understanding of the archive's content just months following the suppression of the tribunal on the island. The Maltese archive is among the major ones to survive to very substantial extent, even more so since the archive of the Congregation for the Doctrine of the Faith (formerly the Congregation of the Roman Inquisition) also holds a substantial collection of Malta material

Undulant fever spondylitis

The general clinical picture of undulant fever is described in this article. The historical and pathological aspects of this disease are briefly reviewed. The radiological picture of Brucellar spondylitis is based on the review of 62 cases of undulant fever, which showed radiographic evidence of spine involvement. The main changes are destructive lesions in the vertebral bodies, usually marginal, with characteristic sclerotic reactions around them and proliferative marginal new bone formations resembling calcifications of ligaments or osteophytic processes. The disc space is usually narrowed. Signs of cord compression were present in eight cases. The differential diagnosis is briefly discussed. Treatment is conservative and in most cases complete cure results.peer-reviewe

Defining and Identifying Satellite Cell-opathies within Muscular Dystrophies and Myopathies

Muscular dystrophies and congenital myopathies arise from specific genetic mutations causing skeletal muscle weakness that reduces quality of life. Muscle health relies on resident muscle stem cells called satellite cells, which enable life-course muscle growth, maintenance, repair and regeneration. Such tuned plasticity gradually diminishes in muscle diseases, suggesting compromised satellite cell function. A central issue however, is whether the pathogenic mutation perturbs satellite cell function directly and/or indirectly via an increasingly hostile microenvironment as disease progresses. Here, we explore the effects on satellite cell function of pathogenic mutations in genes (myopathogenes) that associate with muscle disorders, to evaluate muscle pathological hallmarks that define dysfunctional satellite cells. We deploy transcriptomic analysis and comparison between muscular dystrophies and myopathies to determine the contribution of satellite cell dysfunction using literature, expression dynamics of myopathogenes and correlation with expression of the satellite cell marker PAX7. Our multimodal approach extends current pathological classifications to define Satellite Cell-opathies: muscle disorders in which satellite cell dysfunction contributes to pathology. Primary Satellite Cell-opathies are conditions where mutations in a myopathogene directly affect satellite cell function, such as in Progressive Congenital Myopathy with Scoliosis (MYOSCO) and Carey-Fineman-Ziter Syndrome (CFZS). Primary satellite cell-opathies are generally characterised as being congenital with general hypotonia, and specific involvement of respiratory, trunk and facial muscles, although serum CK levels are usually within the normal range. Secondary Satellite Cell-opathies have mutations in myopathogene that affect both satellite cells and muscle fibres. Such classification aids diagnosis and predicting probable disease course, as well as informing on treatment and therapeutic development

Photopyroelectric Investigation of the trans–cis Isomerization Effect on the Nematic- Isotropic Phase Transition of a Liquid Crystalline Azobenzene

Photopyroelectric calorimetry has been applied to the study of the effects of different concentration of photo-induced cis isomeric molecules on the characteristics of the nematic – isotropic phase transition in p,p’-diheptylazobenzene (7AB) samples. The investigations could be carried out by monitoring the behavior of the thermal diffusivity over the phase transition before and during the sample irradiation with UV light of varying intensity. Among other, it is shown that the monitoring of the time dependence of the thermal diffusivity at fixed sample temperature enabled the comparative study of the dynamics of the trans–cis and cis–trans isomeric transitions. It was shown that the increasing cis isomers concentration caused an increase in the fraction of material undergoing the phase transitions and increased the cis–trans conversion rate. Finally, polarization microscopy observations of the sample texture, which could be carried out together with the calorimetric evaluations proved useful for a more comprehensive analysis of the obtained results

Thermographic and reflectographic imaging investigations on Baroque paintings preserved at the Chigi Palace in Ariccia

In this work, two different mid-infrared imaging techniques operating in the 3-5 μm spectral range are applied to the study of three paintings on canvas, dating back to the XVII century, preserved at the Chigi Palace in Ariccia (Italy). A combined approach based on the use of pulsed thermography and mid-infrared reflectography is proposed for the analysis of the 'Primavera' by Filippo Lauri and Mario Nuzzi, the 'Ritratto di Mario Nuzzi che dipinge un vaso di fiori' by Giovanni Maria Morandi and Mario Nuzzi and the 'Ebbrezza di Noee' by Andrea Sacchi. The aim is to show how the integrated use of these techniques enables a depth-resolved investigation of the entire layered structure of the paintings, from the surface up to the canvas support. The complementarity of the presented results allows an evaluation of the conservative status of the support and the detection of graphical and pictorial features hidden beneath the surface layer, such as pentimenti, as these features are important for the historical and artistic characterisation of the artefact

The current state of DNA barcoding of macroalgae in the Mediterranean Sea : presently lacking but urgently required

The research work disclosed in this publication is partially funded by the ENDEAVOUR Scholarship Scheme (Malta)-Group B – National Funds. FCK would also like to thank the UK Natural Environment Research Council (grants NE/D521522/1, NE/J023094/1, 2025/WP 4.5), the TOTAL Foundation (Project “Diversity of brown algae in the Eastern Mediterranean”) and the Marine Alliance for Science and Technology for Scotland (MASTS) pooling initiative, which is funded by the Scottish Funding Council (grant reference HR09011) and contributing institutions. AFP was funded by the project IDEALG (France: ANR-10-BTBR-04).Peer reviewedPostprin

Warm temperature acclimation impacts metabolism of paralytic shellfish toxins from Alexandrium minutum in commercial oysters

© 2015 John Wiley & Sons Ltd. Species of Alexandrium produce potent neurotoxins termed paralytic shellfish toxins and are expanding their ranges worldwide, concurrent with increases in sea surface temperature. The metabolism of molluscs is temperature dependent, and increases in ocean temperature may influence both the abundance and distribution of Alexandrium and the dynamics of toxin uptake and depuration in shellfish. Here, we conducted a large-scale study of the effect of temperature on the uptake and depuration of paralytic shellfish toxins in three commercial oysters (Saccostrea glomerata and diploid and triploid Crassostrea gigas, n = 252 per species/ploidy level). Oysters were acclimated to two constant temperatures, reflecting current and predicted climate scenarios (22 and 27 °C), and fed a diet including the paralytic shellfish toxin-producing species Alexandrium minutum. While the oysters fed on A. minutum in similar quantities, concentrations of the toxin analogue GTX1,4 were significantly lower in warm-acclimated S. glomerata and diploid C. gigas after 12 days. Following exposure to A. minutum, toxicity of triploid C. gigas was not affected by temperature. Generally, detoxification rates were reduced in warm-acclimated oysters. The routine metabolism of the oysters was not affected by the toxins, but a significant effect was found at a cellular level in diploid C. gigas. The increasing incidences of Alexandrium blooms worldwide are a challenge for shellfish food safety regulation. Our findings indicate that rising ocean temperatures may reduce paralytic shellfish toxin accumulation in two of the three oyster types; however, they may persist for longer periods in oyster tissue

Dynamics of muscle fibre growth during postnatal mouse development

<p>Abstract</p> <p>Background</p> <p>Postnatal growth in mouse is rapid, with total skeletal muscle mass increasing several-fold in the first few weeks. Muscle growth can be achieved by either an increase in muscle fibre number or an increase in the size of individual myofibres, or a combination of both. Where myofibre hypertrophy during growth requires the addition of new myonuclei, these are supplied by muscle satellite cells, the resident stem cells of skeletal muscle.</p> <p>Results</p> <p>Here, we report on the dynamics of postnatal myofibre growth in the mouse extensor digitorum longus (EDL) muscle, which is essentially composed of fast type II fibres in adult. We found that there was no net gain in myofibre number in the EDL between P7 and P56 (adulthood). However, myofibre cross-sectional area increased by 7.6-fold, and length by 1.9-fold between these ages, resulting in an increase in total myofibre volume of 14.1-fold: showing the extent of myofibre hypertrophy during the postnatal period. To determine how the number of myonuclei changes during this period of intense muscle fibre hypertrophy, we used two complementary mouse models: <it>3F-nlacZ-E </it>mice express <it>nlacZ </it>only in myonuclei, while <it>Myf5</it>^{<it>nlacZ</it>/+}mice have β-galactosidase activity in satellite cells. There was a ~5-fold increase in myonuclear number per myofibre between P3 and P21. Thus myofibre hypertrophy is initially accompanied by a significant addition of myonuclei. Despite this, the estimated myonuclear domain still doubled between P7 and P21 to 9.2 × 10³μm³. There was no further addition of myonuclei from P21, but myofibre volume continued to increase, resulting in an estimated ~3-fold expansion of the myonuclear domain to 26.5 × 10³μm³by P56. We also used our two mouse models to determine the number of satellite cells per myofibre during postnatal growth. Satellite cell number in EDL was initially ~14 satellite cells per myofibre at P7, but then fell to reach the adult level of ~5 by P21.</p> <p>Conclusions</p> <p>Postnatal fast muscle fibre type growth is divided into distinct phases in mouse EDL: myofibre hypertrophy is initially supported by a rapid increase in the number of myonuclei, but nuclear addition stops around P21. Since the significant myofibre hypertrophy from P21 to adulthood occurs without the net addition of new myonuclei, a considerable expansion of the myonuclear domain results. Satellite cell numbers are initially stable, but then decrease to reach the adult level by P21. Thus the adult number of both myonuclei and satellite cells is already established by three weeks of postnatal growth in mouse.</p

Proteomic responses to gold(III)-toxicity in the bacterium Cupriavidus metallidurans CH34

Accepted 11th October 2016The metal-resistant β-proteobacterium Cupriavidus metallidurans drives gold (Au) biomineralisation and the (trans)formation of Au nuggets largely via unknown biochemical processes, ultimately leading to the reductive precipitation of mobile, toxic Au(i/iii)-complexes. In this study proteomic responses of C. metallidurans CH34 to mobile, toxic Au(iii)-chloride are investigated. Cells were grown in the presence of 10 and 50 μM Au(iii)-chloride, 50 μM Cu(ii)-chloride and without additional metals. Differentially expressed proteins were detected by difference gel electrophoresis and identified by liquid chromatography coupled mass spectrometry. Proteins that were more abundant in the presence of Au(iii)-chloride are involved in a range of important cellular functions, e.g., metabolic activities, transcriptional regulation, efflux and metal transport. To identify Au-binding proteins, protein extracts were separated by native 2D gel electrophoresis and Au in protein spots was detected by laser absorption inductively coupled plasma mass spectrometry. A chaperon protein commonly understood to bind copper (Cu), CupC, was identified and shown to bind Au. This indicates that it forms part of a multi-metal detoxification system and suggests that similar/shared detoxification pathways for Au and Cu exist. Overall, this means that C. metallidurans CH34 is able to mollify the toxic effects of cytoplasmic Au(iii) by sequestering this Au-species. This effect may in the future be used to develop CupC-based biosensing capabilities for the in-field detection of Au in exploration samples.Carla M. Zammit, Florian Weiland, Joël Brugger, Benjamin Wade, Lyron Juan Winderbaum, Dietrich H. Nies, Gordon Southam, Peter Hoffmann and Frank Reit

Population, ecology and genetic characteristics of the Mediterrannean box jellyfish Carybdea Marsupialis in the island of Malta

The main aims of this study were to investigate the environmental parameters associated with blooming events of Carybdea marsupialis and to genetically compare the box jellyfish in Malta to other Mediterranean and Atlantic samples. The numbers of adult individuals, as well as abiotic and biotic factors were monitored in situ and analysed statistically. Phenological patterns were determined and the abundance of C. marsupialis at the Maltese sites was shown to be strongly and positively correlated with sea water temperature. Genetic analysis indicated a high degree of homology between the sequences derived from the analysed Mediterranean specimens, which was not apparent in the genetic material derived from specimens from the Eastern Atlantic (Cadiz).peer-reviewe