Testing of Drilled Shafts Socketed Into Limestone

Construction of the first phase of the South Parking Garage at the Tampa International Airport in Florida was completed in December 1991. A full-scale field load testing program was used in design of the drilled shaft foundations, with specific goals of determining the bond strength and characterizing the load-displacement behavior of the rock sockets. The displacement behavior and bond strength of the test shafts were predicted from elastic solutions and semiempirical methods. Input parameters included the rock uniaxial compressive strength and elastic modulus. The load test results are compared to the predictions herein, and the practical application of these comparisons is demonstrated by a sample evaluation of a drilled shaft supporting the South Parking Garage

Imagining futures in changing locales : de-industrialisation and education work interfaces

The study investigated men and women's engagement with education in two South Wales valley communities across three generations. It drew upon theoretical tools from social representations theory (Moscovici, 1984) to map dominant social representations circulating in two South Wales valley communities—Abertillery and Ynysawdre. Both communities had experienced a traumatic rupture in community life due to the closure of coal mining in their locales. This study understands social representations as belonging to communities, anchored in the strategies and practices that are particular to that locale and its specific history, and recognises that they influence action and interaction. A snowball sampling method was used to recruit men and women of three generations—16–25 years, 30–45 years and 50–70 years—who were given two research instruments. The first instrument was a semi-structured photo-elicitation interview. The photographs re-presented aspects of the visual and material culture of the locale. Questions related to the locale and to experiences of education and work. The second instrument was an unstructured interview that elicited experiences of education and work. A thematic analysis identified themes, or social representations, that related to the dominant social representations of education and work. Although individual accounts were elicited, differences in the appropriation of social representations and the meanings they held were found between locales, between generations and between genders. Data from both instruments allowed the structure of social representations within each locale to be mapped, which illustrated gender differences and change across generations. A narrative analysis of individual accounts demonstrated how social representations circulating in the locale became psychically active and acted as symbolic resources as individuals and groups made sense of the rupture in the community. Local communities constrain and facilitate what is available for constructing narrative accounts and social identities in relation to education and work, which shift across time.EThOS - Electronic Theses Online ServiceGBUnited Kingdo

Mechanism of action of the monosialoganglioside GM1 as a modulator of CD4 expression. Evidence that GM1-CD4 interaction triggers dissociation of p56lck from CD4, and CD4 internalization and degradation.

Analyzing the mechanisms underlying the capability of the monosialoganglioside GM1 to induce CD4 modulation we observed that GM1 has a dual effect on the CD4 molecule. GM1 treatment of the lymphoma cell line MOLT-3 and CD4-transfected HeLa cells for times shorter than 30 min prevented binding of monoclonal antibodies (mAbs) recognizing epitopes located within the first NH2-terminal domains of CD4, but not of the OKT4 mAb, which binds to the region of CD4 proximal to the transmembrane domain. However, no binding of the OKT4 mAb was observed after a few hours of treatment with GM1 in both MOLT-3 cells and HeLa cells transfected with an intact CD4 molecule, but not in HeLa cells transfected with a CD4 molecule lacking the bulk of the cytoplasmic domain, suggesting that modulation of CD4 by GM1 depends on the integrity of the cytoplasmic domain. GM1 treatment blocked binding of several mAbs which recognize epitopes located within the first two NH2-terminal domains of CD4 and did not induce CD4 down-modulation if MOLT-3 cells were preincubated with the OKT4A or the OKT4 mAbs. Immunoprecipitation studies with [35S]methionine-labeled MOLT-3 cells showed that GM1-induced CD4 down-modulation was accompanied by CD4 degradation, and this was preceded by dissociation of p56lck from CD4. GM1-induced CD4 down-modulation, dissociation of p56lck from CD4, and CD4 degradation were unaffected by staurosporine, which, on the contrary, blocked these events in response to phorbol 12-myristate 13-acetate. These observations demonstrate that the first action of GM1 is to mask epitopes located within the first two NH2-terminal domains; then, GM1 triggers protein kinase C-independent signals which cause p56lck dissociation from CD4 and the delivery of the molecule to an intracellular compartment where it is eventually degraded

Detection and verification of malting quality QTLs using wild barley introgression lines

A malting quality quantitative trait locus (QTL) study was conducted using a set of 39 wild barley introgression lines (hereafter abbreviated with S42ILs). Each S42IL harbors a single marker-defined chromosomal segment from the wild barley accession ‘ISR 42-8’ (Hordeum vulgare ssp. spontaneum) within the genetic background of the elite spring barley cultivar ‘Scarlett’ (Hordeum vulgare ssp. vulgare). The aim of the study was (1) to verify genetic effects previously identified in the advanced backcross population S42, (2) to detect new QTLs, and (3) to identify S42ILs exhibiting multiple QTL effects. For this, grain samples from field tests in three different environments were subjected to micro malting. Subsequently, a line × phenotype association study was performed with the S42ILs in order to localize putative QTL effects. A QTL was accepted if the trait value of a particular S42IL was significantly (P < 0.05) different from the recurrent parent as a control, either across all tested environments or in a particular environment. For eight malting quality traits, altogether 40 QTLs were localized, among which 35 QTLs (87.5%) were stable across all environments. Six QTLs (15.0%) revealed a trait improving wild barley effect. Out of 36 QTLs detected in a previous advanced backcross QTL study with the parent BC2DH population S42, 18 QTLs (50.0%) could be verified with the S42IL set. For the quality parameters α-amylase activity and Hartong 45°C, all QTLs assessed in population S42 were verified by S42ILs. In addition, eight new QTL effects and 17 QTLs affecting two newly investigated traits were localized. Two QTL clusters harboring simultaneous effects on eight and six traits, respectively, were mapped to chromosomes 1H and 4H. In future, fine-mapping of these QTL regions will be conducted in order to shed further light on the genetic basis of the most interesting QTLs