A discriminating microscopy technique for the measurement of ice crystals and air bubbles size distribution in sorbets

24ième Congrès International du Froid ICR 2015, Yokohama, JPN, 16-/08/2015 - 22/08/2015International audienceIn this work, a technique capable to distinguish between ice crystals and air bubbles in sorbets was developed in order to characterize the effect of operating conditions on their size distributions at the exit of the freezer. A pilot freezer was used to crystallize and aerate a commercial lemon sorbet mix. Crystals and bubbles sizes were measured using a light microscope technique under low temperature in a refrigerated glove box developed in the lab for that purpose. Results showed that the developed microscope technique allowed to distinguish them and to quantify their size distributions. Measurements showed that ice crystals size decreases with air flow rate while air bubbles size increases. The latter also increases with the cylinder pressure inside the scraped surface heat exchanger (SSHE)

Inheritance of fresh seed dormancy in Spanish-type peanut (Arachis hypogaea L.): bias introduced by inadvertent selfed flowers as revealed by microsatellite markers control

Production and seed quality in peanut (Arachis hypogaea L.) can be reduced substantially by in situ germination under unpredictable rainfed environments. Inheritance of fresh seed dormancy in Spanish x Spanish crosses was studied with two sets of segregating populations, an F2 population derived from true F1 hybrids identified with peanut microsatellites markers and other populations (F2, BC1P1S and BC1P2S) from randomly-selected F1 individuals. In the F2 population developed with true F1 hybrids, the chi square test was not significant for the deviation from the expected 3:1 (dormant: non-dormant) ratio. In addition, the bimodal frequency distribution curve with the F2 population gave more evidence that fresh seed dormancy is controlled by a single dominant gene. The average frequency (48%) of true F1 hybrids give evidence that deviations from expected ratios in the populations (F2 and BC1P1S) developed from non-tested F1 individuals, is most likely due to inadvertent selfs. This study emphasized the need to identify with molecular markers the cross progenies in self-pollinated crops as peanutbefore testing for any trait

Effect of arbuscular mycorrhizal fungal inoculation on growth, and nutrient uptake of the two grass species, Leptochloa fusca (L.) Stapf and Sporobolus robustus Kunth, under greenhouse conditions

The aim of our work was to assess the effect of inoculation with three arbuscular mycorrhizal fungi (AMF) (Rhizoglomus aggregatum (N.C. Schenck and G.S. Sm.) Sieverd., G.A. Silva and Oeh., Funneliformis mosseae (T.H. Nicolson and Gerd.) C. Walker and A. Schüssler. and Rhizoglomus intraradices (N.C. Schenck and G.S. Sm.) Sieverd., G.A. Silva and Oehl.), and a mixed inoculum of these AMF on root colonization, biomass production, mycorrhizal dependency (MD) and shoot mineral contents of two salt tolerant grasses Leptochloa fusca L. Stapf and Sporobolus robusts Kunth. After four months of growth in a sterilized soil and greenhouse conditions, grasses inoculated with AMF showed significantly higher total biomass production than non-inoculated seedlings. MD and shoot mineral contents (especially P) varied with AMF host plants. Maximum values of MD (13%) were observed in L. fusca and S. robustus seedlings when inoculated with R. intraradices and F. mosseae, respectively. Only P contents were higher in the S. robustus/mixed-AMF combinations than the other treatments. These results demonstrate the potential benefits in our experimental conditions of AM inoculation for improving growth and P acquisition particularly in the L. fusca/ F. mosseae and S. robustus/mixed-AMF combinations.Key words: Grass species, symbiosis, mycorrhizal dependency, mineral nutrition

Construction of chromosome segmen substitution lines in peanut (Arachis hypogaea L.) using a wild synthetic and QTL mapping for plant morphology.

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Oral rehydration therapies in Senegal, Mali, and Sierra Leone: A spatial analysis of changes over time and implications for policy

Background: Oral rehydration solution (ORS) is a simple intervention that can prevent childhood deaths from severe diarrhea and dehydration. In a previous study, we mapped the use of ORS treatment subnationally and found that ORS coverage increased over time, while the use of home-made alternatives or recommended home fluids (RHF) decreased, in many countries. These patterns were particularly striking within Senegal, Mali, and Sierra Leone. It was unclear, however, whether ORS replaced RHF in these locations or if children were left untreated, and if these patterns were associated with health policy changes.Methods: We used a Bayesian geostatistical model and data from household surveys to map the percentage of children with diarrhea that received (1) any ORS, (2) only RHF, or (3) no oral rehydration treatment between 2000 and 2018. This approach allowed examination of whether RHF was replaced with ORS before and after interventions, policies, and external events that may have impacted healthcare access.Results: We found that RHF was replaced with ORS in most Sierra Leone districts, except those most impacted by the Ebola outbreak. In addition, RHF was replaced in northern but not in southern Mali, and RHF was not replaced anywhere in Senegal. In Senegal, there was no statistical evidence that a national policy promoting ORS use was associated with increases in coverage. In Sierra Leone, ORS coverage increased following a national policy change that abolished health costs for children.Conclusions: Children in parts of Mali and Senegal have been left behind during ORS scale-up. Improved messaging on effective diarrhea treatment and/or increased ORS access such as through reducing treatment costs may be needed to prevent child deaths in these areas

Identification of quantitative trait loci for yield and yield related traits in groundnut (Arachis hypogaea L.) under different water regimes in Niger and Senegal

Yield under drought stress is a highly complex trait with large influence to even a minor fluctuation in the environmental conditions. Genomics-assisted breeding holds great promise for improving such complex traits more efficiently in less time, but requires markers associated with the trait of interest. In this context, a recombinant inbred line mapping population (TAG 24 × ICGV 86031) was used to identify markers associated with quantitative trait loci (QTLs) for yield and yield related traits at two important locations of West Africa under well watered and water stress conditions. Among the traits analyzed under WS condition, the harvest index (HI) and the haulm yield (HYLD) were positively correlated with the pod yield (PYLD) and showed intermediate broad sense heritability. QTL analysis using phenotyping and genotyping data resulted in identification of 52 QTLs. These QTLs had low phenotypic variance (<12 %) for all the nine traits namely plant height, primary branching, SPAD chlorophyll meter reading, percentage of sound mature kernels, 100 kernel weight, shelling percentage, HI, HYLD and PYLD. Interestingly, few QTLs identified in this study were also overlapped with previously reported QTLs detected for drought tolerance related traits identified earlier in Indian environmental conditions using the same mapping population. Accumulating these many small-effect QTLs into a single genetic background is nearly impossible through marker-assisted backcrossing and even marker-assisted recurrent selection. Under such circumstances, the deployment of genomic selection is the most appropriate approach for improving such complex traits with more precision and in less time

COMBINING ABILITY AND HETEROTIC PATTERN IN WEST AFRICAN SORGHUM LANDRACES

Heterotic grouping based on combining ability for traits of interest, in addition to plant performance and genetic relationship among germplasm, would be useful in planning crosses in breeding programmes. This study aimed at determining the combining abilities of selected landraces for morphological and physiological traits under contrasted environments and identifying the suitable heterotic grouping method for superior hybrid production. For this purpose, nineteen accessions representing different origins and population structure among West African Sorghum Accessions Panel (WASAP) and two female lines, were used in \uab\ua0Line x tester\ua0\ubb mating design to produce thirty-eight (38) hybrids. Analysis of variance showed significant general combining ability and specific combining ability effects for grain weight, plant height, days to flowering, panicle length and chlorophyll content implying existence of heterotic responses for these traits. Both additive and non-additive gene effects were important in controlling the inheritance of the traits. Parents K31.3, K37 and Ni331 were the best combiners for grain weight, providing the opportunity for breeders to improve grain yield under diverse environments. AVG-1 x K31.3 and AVG-1 x Tg148 were the superior hybrids. Combining ability based heterotic grouping methods classified sorghum accessions into four groups. The heterotic group\u2019s specific and general combining ability (HSGCA) method was the suitable in predicting hybrid superiority. This combining abilities based heterotic grouping study is an additional tool that breeders could use to identify the best parents for superior hybrids development in West Africa.La d\ue9termination des groupes h\ue9t\ue9rotiques bas\ue9e sur les aptitudes \ue0 la combinaison des traits d\u2019int\ue9r\ueat en plus des performances et des relations g\ue9n\ue9tiques dans une collection est importante pour planifier les croisements en s\ue9lection. Cette visait la d\ue9termination des aptitudes \ue0 la combinaison des accessions locales pour certains traits morphologiques sous environnements contrast\ue9s et l\u2019identification de la meilleure m\ue9thode dans la pr\ue9diction de la vigueur hybride. Dans cette perspective, dix-neuf accessions repr\ue9sentant diff\ue9rentes origines et la structure de la population d\u2019un panel ouest africain de sorgho (WASAP) et deux m\ue2les st\ue9riles ont \ue9t\ue9 utilis\ue9s dans un croisement \uab\ua0Line x tester\ua0\ubb pour produire trente-huit hybrides F1, objet de cette \ue9tude. L\u2019analyse de variance a indiqu\ue9 des effets significatifs de l\u2019aptitude g\ue9n\ue9rale et sp\ue9cifique \ue0 la combinaison pour le poids grain, la hauteur plante, la dur\ue9e semis floraison et la concentration en chlorophylle traduisant l\u2019existence des r\ue9ponses h\ue9t\ue9rotiques pour ces traits. Les effets g\ue9niques additif et non-additif ont \ue9t\ue9 importants dans le contr\uf4le de ces caract\ue8res. K31.3, K37 et Ni331 pr\ue9sentent les meilleures aptitudes g\ue9n\ue9rales \ue0 la combinaison puis AVG-1 x K31.3 et AVG-1 x Tg148 ont \ue9t\ue9 les meilleurs hybrides pour le poids grains. Ils constituent une opportunit\ue9 pour l\u2019am\ue9lioration dans divers environnements. Les diff\ue9rentes m\ue9thodes ont class\ue9 les accessions de sorgho en quatre groupes. Le groupage bas\ue9 sur la m\ue9thode HSGCA accroit l\u2019efficience en s\ue9lection de 15 %. Cette \ue9tude offre un outil d\u2019aide pour les s\ue9lectionneurs dans le choix des meilleurs parents \ue0 croiser pour la production des hybrides sup\ue9rieurs en Afrique de l\u2019Ouest