Produção sementes de cenoura.

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Biolistic-mediated genetic transformation of cowpea (Vigna unguiculata) and stable Mendelian inheritance of transgenes.

Made available in DSpace on 2018-05-24T00:41:09Z (GMT). No. of bitstreams: 1 SP19591ID30693.pdf: 573782 bytes, checksum: 13f83a2f9dcd4695291802338c95159d (MD5) Previous issue date: 2009-01-1

Depression Among Portuguese Pregnant Women During Covid-19 Lockdown: A Cross Sectional Study

Introduction: Coronavirus disease 2019 was declared as a pandemic on March 2020. Research on its psychological effects is still lacking. Perinatal depression is a medical complication of pregnancy, especially in situations of stress. In this study, we aimed to investigate the presence of symptoms of depression in pregnant women during the lockdown period in Portugal. Methods: This study consisted in a cross-sectional study among Portuguese pregnant women, who completed an online self-report questionnaire between 25th April and 30th April 2020. An anonymous online questionnaire was developed to assess depression and concerns related to COVID-19. This study was approved by the IRB of Hospital Dona Estefânia and performed in accordance with the ethical standards laid down in the 1964 Declaration of Helsinki. Eligibility criteria included pregnant women, ≥ 18 years and living in Portugal. The primary outcome was to evaluate the presence of depressive symptoms and its association to socio-demographic characteristics and to concerns related to COVID-19. Results: A total of 1698 pregnant women were enrolled. The mean age was 31.9 years. 82.4% felt a negative impact of the pandemic in the surveillance of pregnancy and 43% felt insufficient support. 26.3% showed "possible depression" according to the EPDS. A regression analysis revealed the possibility of depression increased as the concerns about COVID increased and was lower for women with support. The possibility of depression was higher for women with psychiatric medical history. Conclusion: This study demonstrated a significant increase in clinically significant depressive symptoms in pregnant women during the lockdown. It also revealed some of the socio-demographic characteristics of women at risk for depression. If left untreated, depression tends to persist, affecting the woman and also the child. Our findings suggest that COVID-19 represents a serious challenge for this population and reinforce the urgent need for early detection and intervention on mental health issues during pregnancy, especially during the pandemic.info:eu-repo/semantics/publishedVersio

Decision Capacity Assessment in Consultation-Liaison Psychiatry

Introdução: Entre as funções do psiquiatra de ligação, encontra-se a avaliação da capacidade de decidir. Esta envolve a capacidade de fazer escolhas de forma autónoma e surge de forma relevante na prática clínica diária como pré-condição ao consentimento informado. Objetivos: Os autores pretendem rever o processo subjacente à avaliação da capacidade de decisão, assim como outra informação relevante publicada a este respeito. Métodos: Revisão não sistemática da literatura através da pesquisa eletrónica nos motores de busca Medline/Pubmed. Resultados: A capacidade de decidir apenas pode ser avaliada relativamente a uma decisão em particular, sendo que o seu resultado não é necessariamente estável ao longo do tempo. Da mesma forma, não existe nenhum diagnóstico que permita concluir, por si só, pela incapacidade para decidir ou que possibilite prescindir da sua avaliação. Depende de vários fatores: conteúdo, forma do pensamento e funções cognitivas. Pode igualmente ser influenciada pelo nível de instrução do indivíduo, traços da personalidade, estados emocionais/mecanismos de coping ou fatores circunstanciais. Qualquer médico deverá estar habilitado para a realização desta avaliação. O psiquiatra de ligação deve ser envolvido perante a suspeita de doença mental capaz de prejudicar a capacidade de consentir ou perante a necessidade de validar uma avaliação já realizada. Appelbaum e Grisso propuseram uma avaliação sistematizada segundo os seguintes critérios: a) comunicação da escolha, b) compreensão, c) apreciação e d) processo de decisão racional/ raciocínio. Numa tentativa de minimizar diferenças entre avaliadores, foram desenvolvidos vários instrumentos de avaliação, entre os quais se destaca a MacArthur Competence Assessment Tool. Particularmente nos doentes com défice cognitivo, tem sido também utilizada o Mini Mental State Examination, surgindo propostas no sentido de correlacionar os scores obtidos com a presença de capacidade de decisão. Perante a incapacidade para decidir, deverá ser respeitado o melhor interesse do doente, sendo procurada uma “decisão de substituição”, no rigoroso respeito pela ética e pela lei. Conclusões: Um doente capaz de decidir deve apresentar: a) atenção focada no problema; b) capacidade de considerar as diferentes opções propostas; c) capacidade de avaliar os riscos e benefícios e d) capacidade de antecipar os seus possíveis resultados.info:eu-repo/semantics/publishedVersio

Cross-cultural adaptation and psychometric properties of the Brazilian coach-athlete relationship questionnaire (CART-Q), athlete Version

The aim of this study was to test the psychometric properties of the Brazilian version of the Coach-Athlete Relationship Questionnaire (CART-Q)-Athlete Version. For this, three studies were performed. In the first, four translators and five experts in Sport Psychology adapted the CART-Q contents to the Brazilian context. In the second, 364 athletes of individual and collective sports answered the adapted version of CART-Q. In the third, an independent sample of 185 athletes answered the CART-Q and the Task and Ego Orientation in Sport Questionnaire (TEOSQ) to analyze the external validity of the instrument; and 50 athletes answered the CART-Q in two distinct moments for the analysis of the temporal stability. Study 1 showed that the Portuguese version contains clear and relevant questions (CVC> 0.80). Study 2 showed that the CART-Q presents satisfactory internal consistency (a> 0.70/CC> 0.70). The confirmatory factor analysis showed that the model with 11 items showed good fit [X2/gl = 3.03; CFI = 0.96, GFI =0 94; TLI = 0.94; RMSEA = 0.08] and also the existence of the second-order model. Study 3 showed the external (r> 0.40 with variable task orientation) and internal validity (CFA with an independent sample) and temporal stability (ICC> 0.70). It was concluded that the Brazilian version for of CART-Q proved to be valid to evaluate the perception of athletes about their relationship with the coach in the Brazilian sports context

Investigação sorológica de espécies de Ehrlichia em cães, equinos e humanos de um assentamento rural do sul do Brasil

Objetivou-se determinar a soroprevalência de Ehrlichia spp. e os fatores de risco associados a exposição em uma população restrita de cães, cavalos e humanos altamente expostos a picadas de carrapatos em um assentamento rural brasileiro utilizando um teste comercial de ELISA rápido e dois testes de imunofluorescência indireta (IFI) com antígenos brutos de E. canis e E. chaffeensis. Amostras de soro de 132 cães, 16 cavalos e 100 humanos foram utilizadas. Cinquenta e seis/132 (42,4%) cães foram soropositivos para E. canis. Cães > um ano apresentaram mais chance de serem soropositivos para E. canis do que cães ≤ um ano (p =0,0051). Dez/16 (62,5%) e 8/16 (50%) cavalos foram soropositivos pelo ELISA comercial e IFI, respectivamente. Cinco/100 (5%) humanos foramsoropositivos para E. canis e E. chaffeensis. Rhipicephalus sanguineus (n= 291, 97,98%) nos cães e A. cajennense (n = 25, 96,15%) nos cavalos foram os carrapatos mais encontrados. Concluindo, anticorpos anti-Ehrlichia spp. foram encontrados em cavalos; entretanto, a ausência de uma caracterização molecular impede qualquer conclusão sobre agente envolvido. Além disso, a alta soroprevalência de E. canis em cães e a evidência de anticorpos anti-Ehrlichia sp. em humanos, sugere que os casos de erliquiose humana no Brasil possam ser causados por E. canis ou outra espécie intimamente relacionada.The aims of this study were to determine the seroprevalence of Ehrlichia spp. and risk factors for exposure in a restricted population of dogs, horses, and humans highly exposed to tick bites in a Brazilian rural settlement using a commercial ELISA rapid test and two indirect immunofluorescent assays (IFA) with E. canis and E. chaffeensis crude antigens. Serum samples from 132 dogs, 16 horses and 100 humans were used. Fifty-six out of 132 (42.4%) dogs were seropositive for E. canis. Dogs > one year were more likely to be seropositive for E. canis than dogs ≤ one year (p = 0.0051). Ten/16 (62.5%) and 8/16 (50%) horses were seropositive by the commercial ELISA and IFA, respectively. Five out of 100 (5%) humans were seropositive for E. canis and E. chaffeensis. Rhipicephalus sanguineus (n = 291, 97.98%) on dogs and Amblyomma cajennense (n = 25, 96.15%) on horses were the most common ticks found. In conclusion, anti-Ehrlichia spp. antibodies were found in horses; however, the lack of a molecular characterization precludes any conclusion regarding the agent involved. Additionally, the higher seroprevalence of E. canis in dogs and the evidence of anti-Ehrlichia spp. antibodies in humans suggest that human cases of ehrlichiosis in Brazil might be caused by E. canis, or other closely related species

Correlações entre medidas de germinação de sementes de cenoura em temperaturas elevadas.

O objetivo do trabalho foi definir uma metodologia de seleção para aumentar a taxa de germinação de sementes de cenoura sob duas temperaturas (20ºC e 35ºC).bitstream/item/134040/1/BPD-129-INTERNET1.pd

Investigation of Campomanesia Components: A Fruit of Brazilian Cerrado

A survey of chemical composition of the fruit of Campomanesia adamantium used by rural and urban inhabitants of the cities of the Campo Grande, Mato Grosso do Sul State, Brazil, was carried out by inductively coupled plasma-optical emission spectroscopy (ICP-OES) aiming at the detection of minerals. Fifteen minerals were detected in the peel, pulp, and seeds of plant. The concentrations of elements K, Ca, Na, and P are found to be present at the major level in peel, pulp, and seeds of fruit. The zinc concentration is very low compared to other detected elements. The levels of some chemical elements in the fruit do not exceed the limits established by international legislation. Animal studies should be performed. The knowledge of the chemical elements in plants has economic interest, and involves global health problem