Modular detergents tailor the purification and structural analysis of membrane proteins including G-protein coupled receptors

Detergents enable the purification of membrane proteins and are indispensable reagents instructural biology. Even though a large variety of detergents have been developed in the lastcentury, the challenge remains to identify guidelines that allowfine-tuning of detergents forindividual applications in membrane protein research. Addressing this challenge, here weintroduce the family of oligoglycerol detergents (OGDs). Native mass spectrometry (MS)reveals that the modular OGD architecture offers the ability to control protein purificationand to preserve interactions with native membrane lipids during purification. In addition to abroad range of bacterial membrane proteins, OGDs also enable the purification and analysisof a functional G-protein coupled receptor (GPCR). Moreover, given the modular design ofthese detergents, we anticipatefine-tuning of their properties for specific applications instructural biology. Seen from a broader perspective, this represents a significant advance forthe investigation of membrane proteins and their interactions with lipids

Stabilization of test particles in Induced Matter Kaluza-Klein theory

The stability conditions for the motion of classical test particles in an $n$-dimensional Induced Matter Kaluza-Klein theory is studied. We show that stabilization requires a variance of the strong energy condition for the induced matter to hold and that it is related to the hierarchy problem. Stabilization of test particles in a FRW universe is also discussed.Comment: 15 pages, 1 figure, to appear in Class. Quantum Gra

Seminal magnetic fields from Inflato-electromagnetic Inflation

We extend some previous attempts to explain the origin and evolution of primordial magnetic fields during inflation induced from a 5D vacuum. We show that the usual quantum fluctuations of a generalized 5D electromagnetic field cannot provide us with the desired magnetic seeds. We show that special fields without propagation on the extra non-compact dimension are needed to arrive to appreciable magnetic strengths. We also identify a new magnetic tensor field $B_{ij}$ in this kind of extra dimensional theories. Our results are in very good agreement with observational requirements, in particular from TeV Blazars and CMB radiation limits we obtain that primordial cosmological magnetic fields should be close scale invariance.Comment: Improved version. arXiv admin note: text overlap with arXiv:1007.3891 by other author

Barbero-Immirzi parameter, manifold invariants and Euclidean path integrals

The Barbero-Immirzi parameter $\gamma$ appears in the \emph{real} connection formulation of gravity in terms of the Ashtekar variables, and gives rise to a one-parameter quantization ambiguity in Loop Quantum Gravity. In this paper we investigate the conditions under which $\gamma$ will have physical effects in Euclidean Quantum Gravity. This is done by constructing a well-defined Euclidean path integral for the Holst action with non-zero cosmological constant on a manifold with boundary. We find that two general conditions must be satisfied by the spacetime manifold in order for the Holst action and its surface integral to be non-zero: (i) the metric has to be non-diagonalizable; (ii) the Pontryagin number of the manifold has to be non-zero. The latter is a strong topological condition, and rules out many of the known solutions to the Einstein field equations. This result leads us to evaluate the on-shell first-order Holst action and corresponding Euclidean partition function on the Taub-NUT-ADS solution. We find that $\gamma$ shows up as a finite rotation of the on-shell partition function which corresponds to shifts in the energy and entropy of the NUT charge. In an appendix we also evaluate the Holst action on the Taub-NUT and Taub-bolt solutions in flat spacetime and find that in that case as well $\gamma$ shows up in the energy and entropy of the NUT and bolt charges. We also present an example whereby the Euler characteristic of the manifold has a non-trivial effect on black-hole mergers.Comment: 18 pages; v2: references added; to appear in Classical and Quantum Gravity; v3: typos corrected; minor revisions to match published versio

Modular detergents tailor the purification and structural analysis of membrane proteins including G-protein coupled receptors

Detergents enable the purification of membrane proteins and are indispensable reagents in structural biology. Even though a large variety of detergents have been developed in the last century, the challenge remains to identify guidelines that allow fine-tuning of detergents for individual applications in membrane protein research. Addressing this challenge, here we introduce the family of oligoglycerol detergents (OGDs). Native mass spectrometry (MS) reveals that the modular OGD architecture offers the ability to control protein purification and to preserve interactions with native membrane lipids during purification. In addition to a broad range of bacterial membrane proteins, OGDs also enable the purification and analysis of a functional G-protein coupled receptor (GPCR). Moreover, given the modular design of these detergents, we anticipate fine-tuning of their properties for specific applications in structural biology. Seen from a broader perspective, this represents a significant advance for the investigation of membrane proteins and their interactions with lipids

On Bouncing Brane-Worlds, S-branes and Branonium Cosmology

We present several higher-dimensional spacetimes for which observers living on 3-branes experience an induced metric which bounces. The classes of examples include boundary branes on generalised S-brane backgrounds and probe branes in D-brane/anti D-brane systems. The bounces we consider normally would be expected to require an energy density which violates the weak energy condition, and for our co-dimension one examples this is attributable to bulk curvature terms in the effective Friedmann equation. We examine the features of the acceleration which provides the bounce, including in some cases the existence of positive acceleration without event horizons, and we give a geometrical interpretation for it. We discuss the stability of the solutions from the point of view of both the brane and the bulk. Some of our examples appear to be stable from the bulk point of view, suggesting the possible existence of stable bouncing cosmologies within the brane-world framework.Comment: 35 pages, 7 figures, JHEP style. Title changed and references adde

Phase-space and Black Hole Entropy of Higher Genus Horizons in Loop Quantum Gravity

In the context of loop quantum gravity, we construct the phase-space of isolated horizons with genus greater than 0. Within the loop quantum gravity framework, these horizons are described by genus g surfaces with N punctures and the dimension of the corresponding phase-space is calculated including the genus cycles as degrees of freedom. From this, the black hole entropy can be calculated by counting the microstates which correspond to a black hole of fixed area. We find that the leading term agrees with the A/4 law and that the sub-leading contribution is modified by the genus cycles.Comment: 22 pages, 9 figures. References updated. Minor changes to match version to appear in Class. Quant. Gra

Search for new phenomena with the MT2 variable in the all-hadronic final state produced in proton-proton collisions at s=13 TeV.

A search for new phenomena is performed using events with jets and significant transverse momentum imbalance, as inferred through the MT2 variable. The results are based on a sample of proton-proton collisions collected in 2016 at a center-of-mass energy of 13 TeV with the CMS detector and corresponding to an integrated luminosity of 35.9 fb-1 . No excess event yield is observed above the predicted standard model background, and the results are interpreted as exclusion limits at 95% confidence level on the masses of predicted particles in a variety of simplified models of R-parity conserving supersymmetry. Depending on the details of the model, 95% confidence level lower limits on the gluino (light-flavor squark) masses are placed up to 2025 (1550) GeV . Mass limits as high as 1070 (1175) GeV are set on the masses of top (bottom) squarks. Information is provided to enable re-interpretation of these results, including model-independent limits on the number of non-standard model events for a set of simplified, inclusive search regions

Measurement of double-differential cross sections for top quark pair production in pp collisions at [Formula: see text][Formula: see text] and impact on parton distribution functions.

Normalized double-differential cross sections for top quark pair ([Formula: see text]) production are measured in pp collisions at a centre-of-mass energy of 8[Formula: see text] with the CMS experiment at the LHC. The analyzed data correspond to an integrated luminosity of 19.7[Formula: see text]. The measurement is performed in the dilepton [Formula: see text] final state. The [Formula: see text] cross section is determined as a function of various pairs of observables characterizing the kinematics of the top quark and [Formula: see text] system. The data are compared to calculations using perturbative quantum chromodynamics at next-to-leading and approximate next-to-next-to-leading orders. They are also compared to predictions of Monte Carlo event generators that complement fixed-order computations with parton showers, hadronization, and multiple-parton interactions. Overall agreement is observed with the predictions, which is improved when the latest global sets of proton parton distribution functions are used. The inclusion of the measured [Formula: see text] cross sections in a fit of parametrized parton distribution functions is shown to have significant impact on the gluon distribution