Bioactive Properties, Fatty Acid Composition and Mineral Contents of Grape Seed and Oils

The oil content of grape seed samples changed between 4.53% (Adakarası) and 11.13% (Sauvignon blanc). While the linoleic acid contents of grape seed oils ranged from 47.34% (Sangiovese) to 72.91% (Cinsaut), the oleic acid contents varied between 13.35% (Cabernet Sauvignon) and 26.30% (Sangiovese) (p < 0.05). Also, the palmitic acid and stearic acid contents of grape seed oils ranged from 7.15% (Cinsaut) to 16.06% (Sangiovese) and from 2.43% (Narince) to 6.55% (Sangiovese) respectively (p < 0.05). The flavonoid contents of the seeds changed between 263.53 (mg CE/g) and 1 706.00 (mg CE/g) (Cabernet Sauvignon). Total phenols were found to be between 6 711.14 (mg GA/g extract) (Sangiovese) and 8 818.69 (mg GA/g extract) (Narince). The linoleic contents of oils changed from between 47.34% (Sangiovese) and 72.91% (Cinsaut). The K contents of the grape seed samples changed between 4 347.80 mg/kg (Cabernet Sauvignon) and 9 492.60 mg/kg (Gamay) (p < 0.05). The Fe contents of seeds were found to be between 29.96 mg/kg (Narince) and 73.82 mg/kg (Sangiovese). As a result, the current study shows that grape seeds are useful for human nutrition due to their components

Boron stress exposes differential antioxidant responses in maize cultivars (Zea mays L.)

Boron stress is largely influencing the growth and yield of crop plants all around the world. In this scenario, identification of the genotypes that are tolerant to boron stress, understanding the mechanisms behind the tolerance and their application in the breeding programs can open new pathways towards dealing with this global stress. Focusing on this, we compared the differences in the physiological and biochemical responses of two hybrid maize (Zea mays L.) cultivars, boron tolerant RX 770 and boron susceptible TTM 8119. Both genotypes were subjected to four different B treatments, 0, 2.5, 25 and 50 mg L-1 B. Samples were collected before the application, after 5 days of treatments and 10 days of treatments. Root shoot lengths, dry weights, malondialdehyde (MDA), proline content and activity of antioxidant enzymes were evaluated for different harvest periods. The aim of the study was to determine the role of antioxidant enzymes in providing tolerance to maize genotypes towards B stress. Based on the results, it can be concluded that the activity of SOD, APX, POX and GR enzymes may have a significant role in providing resistance to the maize cultivar RX 770 towards B toxicity, especially at the early stages of plant development. The comparison of the physiological and biochemical mechanism of the tolerant maize genotype with the susceptible one in the presence of the boron stress may provide deep understanding useful for the development of new B tolerant maize cultivars

Chromosomal and oxidative DNA damage in non-functioning pituitary adenomas.

Introduction: Clinically non-functioning pituitary adenomas (NFPA) are common tumours of the pituitary gland and are mainly considered as benign. The primary aim of this study was to research the effects of NFPA on genome instability in patients with non-functioning pituitary adenoma by using the cytokinesis-block micronucleus cytome (CBMN-cyt) assay and 8-hydroxy-2'-deoxyguanosine (8-OHdG) assay. The second objective of this study was to assess whether there is a relationship between age, pituitary adenoma diameters, 8-OHdG levels, CBMN site assay parameters, and tumour aggressiveness

Increased micronucleus, nucleoplasmic bridge, nuclear bud frequency and oxidative DNA damage associated with prolactin levels and pituitary adenoma diameters in patients with prolactinoma

WOS: 000368552800008PubMed: 26720589Prolactinoma is the most common pituitary tumor. Most pituitary tumors are benign, but they often are clinically significant. We investigated cytokinesis-block micronucleus cytome (CBMN cyt) assay parameters and oxidative DNA damage in patients with prolactinoma to assess the relations among age, prolactin level, pituitary adenoma diameter and 8-hydroxy-2'-deoxyguanosine (8-OHdG) level in patients with prolactinoma. We investigated 27 patients diagnosed with prolactinoma and 20 age- and sex-matched healthy controls. We measured CBMN cyt parameters and plasma 8-OHdG levels in peripheral blood lymphocytes of patients with prolactinoma and controls. The frequencies of micronucleus (MN), nucleoplasmic bridge, nuclear bud, apoptotic and necrotic cells, and plasma 8-OHdG levels in patients with prolactinoma were significantly greater than controls. MN frequency was correlated positively with age, prolactin levels and pituitary adenoma diameters in patients with prolactinoma. The increased chromosomal and oxidative DNA damage, and the positive correlation between MN frequency, prolactin levels and pituitary adenoma diameters may be associated with increased risk of cancer in patients with prolactinoma, because increased MN frequency is a predictor of cancer risk.Erciyes University Scientific Research Projects Units [TSD-10-3327]This work was supported by Erciyes University Scientific Research Projects Units (Project number: TSD-10-3327). N. B., H. D-A., F. B., I. C., Z. H., H. D., G. B., S. S. and A. C. D. designed and performed the experiments; N. B., H. D-A. and F. B. analyzed data and wrote the paper