Pulse-Echo Monitoring of Concrete Damage and Spalling during Fire

Monitoring concrete damage and spalling progression in structural members during fire tests (hot conditions) is a central but challenging task, since the high temperatures involved make difficult the implementation of most of the common Non- Destructive evaluation methods. Hence, an advanced ultrasonic technique – Ultrasonic Pulse-Echo (UPE) – was recently adapted for real time survey in fire test, in order to evaluate the material damage during heating. The UPE technique was implemented at the cold (upper) face of concrete slabs (800x800x100 mm) heated at the bottom face according to the Standard Fire and subjected to biaxial compressive membrane loading. Different concretes were tested, with grades ranging from 40 to 60 MPa, with and without different kinds of fibre (monofilament or fibrillated polypropylene, or steel fibres). Furthermore, different load levels were applied, from 0 to 25% of the original compressive strength. During tests, spalling was generally observed in loaded plain concrete (up to 50-60 mm depth), while only slight scaling was experienced on unloaded samples or if polypropylene fibre was added. The method proved to be very effective in recognizing the decay of the Ultrasonic Pulse Velocity (UPV) with temperature and the role played by external loading and fibre type

Decision analysis for the determination of biomass in the territory Tuscia Romana by geographic information system and forest management plans

The growing interest in the development of chains for the use of agroforestry biomass for energy demand, is due to the awareness they are a crucial element to mitigate the global climatic change effects. The true effort is to have a reliable estimation of biomass availability by some instruments like forest management plans, which allow to locate the forest supply and to know the forest biomass availability in a medium period. In this paper we carried out a decision analysis by geographic information system, in Tuscia Romana area comprising 11 municipalities for a total amount of 813 km2. An estimation was carried out taking into account the bibliographic data on the analyzed species, reporting the biomass in weight taken out by the forest cut utilization. A comparison was also performed in field on chestnut trees cut in a sampling area near Bracciano and in a close sawmill. The results show long, medium and short-term dynamics, but some critical points were found related to the process of estimation and to the real procurement of biomass in some years. The results suggest to be care in a possible project of a biomass plant

Diagnostic and prognostic role of renal histopathology in rheumatic diseases

The objective was to evaluate renal involvement in several rheumatic diseases (i.e. rheumatoid arthritis, systemic lupus erythematosus, Sjögren syndrome, systemic sclerosis, systemic vasculitides). The method chosen was to define histopathological profiles reported in renal biopsies performed on patients with renal involvement due to different rheumatic diseases. Renal involvement observed in patients with rheumatic disease can be the direct result of the disease per se and/or a complication of drugs used in the disease treatment. The clinical-pathological correlations derived from the study of renal tissues can be useful for differential diagnosis, prognosis assessment and therapeutic decisions. Renal biopsy should be considered as an important tool for the management of nephropathies in patients with systemic rheumatic diseases

Angiopoietin decoy secreted at tumor site impairs tumor growth and metastases by inducing local inflammation and altering neoangiogenesis

The extracellular domain of the receptor tyrosine kinase Tie2/TEK (exTEK) has been used as an angiopoietin decoy to study the role of angiopoietins in the tumor-host interactions, using a syngeneic model of experimental metastases and subcutaneous tumor. Soluble exTEK secreted by transfected tumor cells inhibited HUVECs from forming tubes in Matrigel. ExTEK-transfected C26 colon carcinoma and TS/A mammary tumor cells displayed reduced growth rate when injected subcutaneously, and reduced ability to form experimental metastases when injected intravenously. Immunohistochemical analysis of tumors and metastases showed increased leukocytes infiltration and signs of inflammation in exTEK-secreting compared to parental tumor, as well as impairment in neo-vessel growth and organization. However, while neoangiogenesis eventually rescued in the subcutis, it failed to organize in the experimental metastases of exTEK-secreting tumor, contributing to the hampering of metastatic growth and to increased mice survival. The reactive infiltrate of C26TEK contained a different percentage of leukocytes and was responsible for the tumor inhibition. In fact, leukopenia induced by gamma-irradiation of recipient mice or injection into interferon gamma (IFN-gamma) gene knockout (GKO) mice resulted in reduced mouse survival and an increased number of lung metastases. On the other hand, interleukin (IL)-12 treatment prolonged the survival of mice bearing subcutaneous C26TEK but not of those bearing lung metastases, suggesting that IL-12 could exert further antiangiogenic effects at the site where the tumor can restore neoangiogenesis. These results show in vivo that reduced angiopoietin availability at the tumor site induces a local inflammatory response and impairment of neoangiogenesis which act synergistically to limit tumor growth and metastasis

HOXB7: a key factor for tumo-associated angiogenic switch.

We had demonstrated previously a functional bridge between altered homebox (HOX) gene expression and tumor progression through HOXB7 transactivation of basic fibroblast growth factor. Here, we have studied whether HOXB7, in addition to basic fibroblast growth factor, may induce other genes directly or indirectly related to neoangiogenesis and tumor invasion. Parental, beta-galactosidase-transduced, and HOXB7-transduced SkBr3 cell lines were examined for the expression of several growth factors and growth factor receptors involved in the proliferative and angiogenic processes. Vascular endothelial growth factor, melanoma growth-stimulatory activity/growth-related oncogenene alpha, interleukin-8, and angiopoietin-2 were up-regulated by HOXB7 transduction. The exception was angiopoietin-1 expression that was abrogated. Additional analyses included the expression levels of enzymes such as matrix metalloprotease (MMP)-2 and MMP-9 and heparanase, capable of proteolytic degradation of extracellular matrix and basement membranes. Results showed an induction of only MMP-9. The functional implication of such a finding was tested using an in vitro coculture assay in a three-dimensional matrix. A delay of differentiation with persistent nests of proliferating cells was found in endothelial cells cocultured with HOXB7-transduced SkBr3 cells. Tumorigenicity of these cells has been evaluated in vivo. Xenograft into athymic nude mice showed that SkBr3/HOXB7 cells developed tumors in mice, either irradiated or not, whereas parental SkBr3 cells did not show any tumor take unless mice were sublethally irradiated. Comparison of tumor nodules for vascularization by CD-31 and CD-34 immunostaining revealed an increased number of blood vessels in tumors expressing HOXB7. Together, the results indicate HOXB7 as a key factor up-regulating a variety of proangiogenic stimuli. Thus, HOXB7 gene or protein is a target to aim at to inhibit tumor-associated neoangiogenesis, considering the number and the redundancy of proangiogenic molecules that should be targeted one by one to theoretically achieve the same effect

Critical parametric quantum sensing

Critical quantum systems are a promising resource for quantum metrology applications, due to the diverging susceptibility developed in proximity of phase transitions. Here, we assess the metrological power of parametric Kerr resonators undergoing driven-dissipative phase transitions. We fully characterize the quantum Fisher information for frequency estimation, and the Helstrom bound for frequency discrimination. By going beyond the asymptotic regime, we show that the Heisenberg precision can be achieved with experimentally reachable parameters. We design protocols that exploit the critical behavior of nonlinear resonators to enhance the precision of quantum magnetometers and the fidelity of superconducting qubit readout.Comment: 6 pages + Supplemental Material, 4 figure

Predictive Role of p53 Protein as a Single Marker or Associated to Ki67 Antigen in Oral Carcinogenesis

p53 over-expression has been proposed as a reliable marker associated to oral carcinogenesis, although only about 50% of oral carcinomas (OSCC) are associated with p53 over-expression and even p53-negative lesions can progress to OSCC. The aim of the study was to determine whether the combination of p53 over-expression and p53 low-expression associated with Ki67 over-expression (high Ki67/p53 ratio) could lead to a more sensitive parameter. Immunohistochemical expression of Ki67 and p53 was measured in 54 specimens from OSCC; 27 specimens from moderate/severe epithelial dysplasia; 32 specimens from oral leukoplakias without epithelial dysplasia, and 13 specimens with normal epithelium. p53 over-expression was found in 31 (53%) samples from OSCC, in 10 (37%) samples from severe dysplasias, and in 5 (15%) samples from non-dysplastic lesions, while the combination of high p53 values with high Ki67/p53 ratio was observed in 93% of OSCC, in 81% of dysplastic lesions, and in 50% of non-dysplastic lesions. This parameter may have a clinical implication to detect early lesions with an impairment of p53 pathway, and probably at risk of progress to OSCC