Comparing heat flow models for interpretation of precast quadratic pile heat exchanger thermal response tests

This paper investigates the applicability of currently available analytical, empirical and numerical heat flow models for interpreting thermal response tests (TRT) of quadratic cross section precast pile heat exchangers. A 3D finite element model (FEM) is utilised for interpreting five TRTs by inverse modelling. The calibrated estimates of soil and concrete thermal conductivity are consistent with independent laboratory measurements. Due to the computational cost of inverting the 3D model, simpler models are utilised in additional calibrations. Interpretations based on semi-empirical pile G-functions yield soil thermal conductivity estimates statistically similar to those obtained from the 3D FEM inverse modelling, given minimum testing times of 60 hours. Reliable estimates of pile thermal resistance can only be obtained from type curves computed with 3D FEM models. This study highlights the potential of applying TRTs for sizing quadratic, precast pile heat exchanger foundations

Interconectividade: professores do Norte e do Sul do Brasil compreendendo saberes pedagógicos

O presente estudo propiciou entendermos como"saberes - o conjunto de conhecimentos e modos"de fazer enraizados no cotidiano das comunidades"- expressam-se nos mais diversos locais,"como mercados, feiras, santuários, praças, escolas"e demais espaços onde se forjam as mentalidades."Tais saberes englobam os rituais e festas"que marcam a vivência coletiva do trabalho, da"religiosidade, da ludicidade, das diversas formas"de expressão artística: literária, musical, cênica,"visual e de outras práticas da vida social. Por"uma atitude científica, somos levados a compreender"as várias dimensões das respostas dos"professores do projeto de pesquisa, realizado em"três municípios da região do Salgado paraense"e articulamos o artigo em tópicos para que cada"um dos saberes fosse aprofundado teoricamente"e entrelaçado com as falas coletadas, assim"chegamos aos subtítulos: Saberes ambientais -"sustentabilidade no nosso planeta; O lúdico na"vida e na escola; O saber poético - sensibilidade"e autoconhecimento; Religiosidade, religião e"espiritualidade. Com aproximações e distanciamentos,"fomos nos reconhecendo nas falas dos"professores da Amazônia paraense, evidenciando"a inseparabilidade de dois contextos, aparentemente"tão distantes e diversos. Professores do"norte do país foram ouvidos por professores do"sul, realidades diversas e semelhantes ao mesmo"tempo. Semelhança em ser professor, semelhança"na busca pelo conhecimento, semelhança no"acolhimento às diferenças, semelhança no amor"pelo ser humano. Semelhanças e diferenças nos"uniram em um mesmo projeto de pesquisa, em"um mesmo projeto de profissão e em um mesmo"projeto de vida. Desta forma, nas considerações"finais, percebemos que estávamos compreendendo"sobre a interconectividade entre professores"do sul e professores do norte e contribuindo para"a construção de saberes em diferentes processos"educativos.Palavras-Chave: Interconectividade - Saberes pedagógicos - Formação de ProfessoresInterconnectivity: teachers of Northern and Southern Brazil understanding pedagogical knowledge" " ""This study led us to understand how knowledge - the set of knowledge and ways of doing rooted in everyday communities - are expressed in various places, such as markets, fairs, sanctuaries, playgrounds, schools and other places where attitudes are forged. Such knowledge encompass rituals and dances which mark the collective experience of work, religiosity, playfulness and other various forms of artistic expression: literary, musical, scenic, visual and other practices of social life. For a scientific attitude, we are driven to understand the various dimensions of teachers' responses to such research project, performed in three municipalities in the region of Salgado Paraense, then we articulated this article on topics for each knowledge were examined theoretically and interlaced with the collected speeches, so we came to the subtitles: Environmental Knowledge - sustainability on our planet; The Playful in Life and in School; Poetic Knowledge - sensitivity and self-awareness; Religion, Religion and Spirituality. Considering similarities and differences, we began to recognize ourselves in the speeches of the teachers of Amazon Paraense, demonstrating the inseparability of these two contexts, seemingly so distant and so diverse. Teachers from the North were heard by teachers from the South, realities which are different and similar at the same time. Similarities in being a teacher, similarities in pursuit of knowledge, similarities to host differences, similarities in love for the human being. Similarities and differences brought us together in a same research project, in a same professional project and in the same project of life. Thus, in concluding remarks, we realized we were comprehending about the interconectivity between Southern and Northern teachers and contributing to the construction of knowledge in different educational processes.Key-words: Interconnectivity - Pedagogical Knowledge - Teacher Training

Towards Prospective Life Cycle Assessment: How to Identify Key Parameters Inducing Most Uncertainties in the Future? Application to Photovoltaic Systems Installed in Spain

The final publication is available at Springer via http://dx.doi.org/10.1007/978-3-319-09150-1_51International audienceProspective Life Cycle Assessment (LCA) is a relevant approach to assess the environmental performance of future energy pathways. Amongst different types of prospective scenarios, cornerstone scenarios meant for complex systems and long-term approaches, are of interest to assess such performance. They rely on different types of long-term projections, such as projections of technological evolutions and of energy resources. In most studies, scenarios are defined with single values for each parameter, and environmental impacts are assessed in a deterministic way. Inherent uncertainties related to these prospective assumptions are not considered and prospective LCA uncertainties are thus not addressed. In this paper we describe a methodology to account for these uncertainties and to identify the parameters inducing most of the uncertainties in the prospective LCA results. We apply this approach to prospective LCAs of photovoltaic-based electricity generation systems

Prodrug converting enzyme gene delivery by L. monocytogenes

<p>Abstract</p> <p>Background</p> <p><it>Listeria monocytogenes </it>is a highly versatile bacterial carrier system for introducing protein, DNA and RNA into mammalian cells. The delivery of tumor antigens with the help of this carrier into tumor-bearing animals has been successfully carried out previously and it was recently reported that <it>L. monocytogenes </it>is able to colonize and replicate within solid tumors after local or even systemic injection.</p> <p>Methods</p> <p>Here we report on the delivery of two prodrug converting enzymes, purine-deoxynucleoside phosphorylase (PNP) and a fusion protein consisting of yeast cytosine deaminase and uracil phosphoribosyl transferase (FCU1) into cancer cells in culture by <it>L. monocytogenes</it>. Transfer of the prodrug converting enzymes was achieved by bacterium mediated transfer of eukaryotic expression plasmids or by secretion of the proteins directly into the host cell cytosol by the infecting bacteria.</p> <p>Results</p> <p>The results indicate that conversion of appropriate prodrugs to toxic drugs in the cancer cells occured after both procedures although <it>L. monocytogenes</it>-mediated bactofection proved to be more efficient than enzyme secretion 4T1, B16 and COS-1 tumor cells. Exchanging the constitutively P_CMV-promoter with the melanoma specific P_4xTETP-promoter resulted in melanoma cell-specific expression of the prodrug converting enzymes but reduced the efficiencies.</p> <p>Conclusion</p> <p>These experiments open the way for bacterium mediated tumor specific activation of prodrugs in live animals with tumors.</p

Morphine activation of mu opioid receptors causes disinhibition of neurons in the ventral tegmental area mediated by β-arrestin2 and c-Src

Abstract The tyrosine kinase, c-Src, participates in mu opioid receptor (MOP) mediated inhibition in sensory neurons in which β-arrestin2 (β-arr2) is implicated in its recruitment. Mice lacking β-arr2 exhibit increased sensitivity to morphine reinforcement; however, whether β-arr2 and/or c-Src participate in the actions of opioids in neurons within the reward pathway is unknown. It is also unclear whether morphine acts exclusively through MOPs, or involves delta opioid receptors (DOPs). We examined the involvement of MOPs, DOPs, β-arr2 and c-Src in the inhibition by morphine of GABAergic inhibitory postsynaptic currents (IPSCs) recorded from neurons in the mouse ventral tegmental area. Morphine inhibited spontaneous IPSC frequency, mainly through MOPs, with only a negligible effect remaining in MOP−/− neurons. However, a reduction in the inhibition by morphine for DOP−/− c.f. WT neurons and a DPDPE-induced decrease of IPSC frequency revealed a role for DOPs. The application of the c-Src inhibitor, PP2, to WT neurons also reduced inhibition by morphine, while the inactive PP3, and the MEK inhibitor, SL327, had no effect. Inhibition of IPSC frequency by morphine was also reduced in β-arr2−/− neurons in which PP2 caused no further reduction. These data suggest that inhibition of IPSCs by morphine involves a β-arr2/c-Src mediated mechanism

A Selectable and Excisable Marker System for the Rapid Creation of Recombinant Poxviruses

Genetic manipulation of poxvirus genomes through attenuation, or insertion of therapeutic genes has led to a number of vector candidates for the treatment of a variety of human diseases. The development of recombinant poxviruses often involves the genomic insertion of a selectable marker for purification and selection purposes. The use of marker genes however inevitably results in a vector that contains unwanted genetic information of no therapeutic value.Here we describe an improved strategy that allows for the creation of marker-free recombinant poxviruses of any species. The Selectable and Excisable Marker (SEM) system incorporates a unique fusion marker gene for the efficient selection of poxvirus recombinants and the Cre/loxP system to facilitate the subsequent removal of the marker. We have defined and characterized this new methodological tool by insertion of a foreign gene into vaccinia virus, with the subsequent removal of the selectable marker. We then analyzed the importance of loxP orientation during Cre recombination, and show that the SEM system can be used to introduce site-specific deletions or inversions into the viral genome. Finally, we demonstrate that the SEM strategy is amenable to other poxviruses, as demonstrated here with the creation of an ectromelia virus recombinant lacking the EVM002 gene.The system described here thus provides a faster, simpler and more efficient means to create clinic-ready recombinant poxviruses for therapeutic gene therapy applications

Model parameterization to simulate and compare the PAR absorption potential of two competing plant species

Mountain pastures dominated by the pasture grass Setaria sphacelata in the Andes of southern Ecuador are heavily infested by southern bracken (Pteridium arachnoideum), a major problem for pasture management. Field observations suggest that bracken might outcompete the grass due to its competitive strength with regard to the absorption of photosynthetically active radiation (PAR). To understand the PAR absorption potential of both species, the aims of the current paper are to (1) parameterize a radiation scheme of a two-big-leaf model by deriving structural (LAI, leaf angle parameter) and optical (leaf albedo, transmittance) plant traits for average individuals from field surveys, (2) to initialize the properly parameterized radiation scheme with realistic global irradiation conditions of the Rio San Francisco Valley in the Andes of southern Ecuador, and (3) to compare the PAR absorption capabilities of both species under typical local weather conditions. Field data show that bracken reveals a slightly higher average leaf area index (LAI) and more horizontally oriented leaves in comparison to Setaria. Spectrometer measurements reveal that bracken and Setaria are characterized by a similar average leaf absorptance. Simulations with the average diurnal course of incoming solar radiation (1998–2005) and the mean leaf–sun geometry reveal that PAR absorption is fairly equal for both species. However, the comparison of typical clear and overcast days show that two parameters, (1) the relation of incoming diffuse and direct irradiance, and (2) the leaf–sun geometry play a major role for PAR absorption in the two-big-leaf approach: Under cloudy sky conditions (mainly diffuse irradiance), PAR absorption is slightly higher for Setaria while under clear sky conditions (mainly direct irradiance), the average bracken individual is characterized by a higher PAR absorption potential. (∼74 MJ m−2 year−1). The latter situation which occurs if the maximum daily irradiance exceeds 615 W m−2 is mainly due to the nearly orthogonal incidence of the direct solar beam onto the horizontally oriented frond area which implies a high amount of direct PAR absorption during the noon maximum of direct irradiance. Such situations of solar irradiance favoring a higher PAR absorptance of bracken occur in ∼36% of the observation period (1998–2005). By considering the annual course of PAR irradiance in the San Francisco Valley, the clear advantage of bracken on clear days (36% of all days) is completely compensated by the slight but more frequent advantage of Setaria under overcast conditions (64% of all days). This means that neither bracken nor Setaria show a distinct advantage in PAR absorption capability under the current climatic conditions of the study area

Endothelial progenitor cells and integrins: adhesive needs

In the last decade there have been multiple studies concerning the contribution of endothelial progenitor cells (EPCs) to new vessel formation in different physiological and pathological settings. The process by which EPCs contribute to new vessel formation in adults is termed postnatal vasculogenesis and occurs via four inter-related steps. They must respond to chemoattractant signals and mobilize from the bone marrow to the peripheral blood; home in on sites of new vessel formation; invade and migrate at the same sites; and differentiate into mature endothelial cells (ECs) and/or regulate pre-existing ECs via paracrine or juxtacrine signals. During these four steps, EPCs interact with different physiological compartments, namely bone marrow, peripheral blood, blood vessels and homing tissues. The success of each step depends on the ability of EPCs to interact, adapt and respond to multiple molecular cues. The present review summarizes the interactions between integrins expressed by EPCs and their ligands: extracellular matrix components and cell surface proteins present at sites of postnatal vasculogenesis. The data summarized here indicate that integrins represent a major molecular determinant of EPC function, with different integrin subunits regulating different steps of EPC biology. Specifically, integrin α4β1 is a key regulator of EPC retention and/or mobilization from the bone marrow, while integrins α5β1, α6β1, αvβ3 and αvβ5 are major determinants of EPC homing, invasion, differentiation and paracrine factor production. β2 integrins are the major regulators of EPC transendothelial migration. The relevance of integrins in EPC biology is also demonstrated by many studies that use extracellular matrix-based scaffolds as a clinical tool to improve the vasculogenic functions of EPCs. We propose that targeted and tissue-specific manipulation of EPC integrin-mediated interactions may be crucial to further improve the usage of this cell population as a relevant clinical agent