Aggregate formation prevents dTDP-43 neurotoxicity in the Drosophila melanogaster eye

TDP-43 inclusions are an important histopathological feature in various neurodegenerative disorders, including Amyotrophic Lateral Sclerosis and Fronto-Temporal Lobar Degeneration. However, the relation of these inclusions with the pathogenesis of the disease is still unclear. In fact, the inclusions could be toxic themselves, induce loss of function by sequestering TDP-43 or a combination of both. Previously, we have developed a cellular model of aggregation using the TDP-43 Q/N rich amino acid sequence 331-369 repeated 12 times (12xQ/N) and have shown that these cellular inclusions are capable of sequestering the endogenous TDP-43 both in non-neuronal and neuronal cells. We have tested this model in vivo in the Drosophila melanogaster eye. The eye structure develops normally in the absence of dTDP-43, a fact previously seen in knock out fly strains. We show here that expression of EGFP 12xQ/N does not alter the structure of the eye. In contrast, TBPH overexpression is neurotoxic and causes necrosis and loss of function of the eye. More important, the neurotoxicity of TBPH can be abolished by its incorporation to the insoluble aggregates induced by EGFP 12xQ/N. This data indicates that aggregation is not toxic per se and instead has a protective role, modulating the functional TBPH available in the tissue. This is an important indication for the possible pathological mechanism in action on ALS patients. © 2014

Non-Markovian non-stationary completely positive open quantum system dynamics

By modeling the interaction of a system with an environment through a renewal approach, we demonstrate that completely positive non-Markovian dynamics may develop some unexplored non-standard statistical properties. The renewal approach is defined by a set of disruptive events, consisting in the action of a completely positive superoperator over the system density matrix. The random time intervals between events are described by an arbitrary waiting-time distribution. We show that, in contrast to the Markovian case, if one performs a system-preparation (measurement) at an arbitrary time, the subsequent evolution of the density matrix evolution is modified. The non-stationary character refers to the absence of an asymptotic master equation even when the preparation is performed at arbitrary long times. In spite of this property, we demonstrate that operator expectation values and operators correlations have the same dynamical structure, establishing the validity of a non-stationary quantum regression hypothesis. The non-stationary property of the dynamic is also analyzed through the response of the system to an external weak perturbation.Comment: 13 pages, 3 figure

Lindblad rate equations

In this paper we derive an extra class of non-Markovian master equations where the system state is written as a sum of auxiliary matrixes whose evolution involve Lindblad contributions with local coupling between all of them, resembling the structure of a classical rate equation. The system dynamics may develops strong non-local effects such as the dependence of the stationary properties with the system initialization. These equations are derived from alternative microscopic interactions, such as complex environments described in a generalized Born-Markov approximation and tripartite system-environment interactions, where extra unobserved degrees of freedom mediates the entanglement between the system and a Markovian reservoir. Conditions that guarantees the completely positive condition of the solution map are found. Quantum stochastic processes that recover the system dynamics in average are formulated. We exemplify our results by analyzing the dynamical action of non-trivial structured dephasing and depolarizing reservoirs over a single qubit.Comment: 12 pages, 2 figure

Thermodynamics of quantum jump trajectories in systems driven by classical fluctuations

The large-deviation method can be used to study the measurement trajectories of open quantum systems. For optical arrangements this formalism allows to describe the long time properties of the (non-equilibrium) photon counting statistics in the context of a (equilibrium) thermodynamic approach defined in terms of dynamical phases and transitions between them in the trajectory space [J.P. Garrahan and I. Lesanovsky, Phys. Rev. Lett. 104, 160601 (2010)]. In this paper, we study the thermodynamic approach for fluorescent systems coupled to complex reservoirs that induce stochastic fluctuations in their dynamical parameters. In a fast modulation limit the thermodynamics corresponds to that of a Markovian two-level system. In a slow modulation limit, the thermodynamic properties are equivalent to those of a finite system that in an infinite-size limit is characterized by a first-order transition. The dynamical phases correspond to different intensity regimes, while the size of the system is measured by the transition rate of the bath fluctuations. As a function of a dimensionless intensive variable, the first and second derivative of the thermodynamic potential develop an abrupt change and a narrow peak respectively. Their scaling properties are consistent with a double-Gaussian probability distribution of the associated extensive variable.Comment: 12 pages, 3 figure

Time-temperature indicator based on the variation of the optical response of photonic crystals upon polymer infiltration

Time-temperature indicators are used for sensing the thermal history of perishable products, like food and pharmaceutical goods. Their working principle is based on a temperature response that mimics the temperature dependence of the deterioration kinetics of a given product. For successful implementation, time-temperature indicators must be of minimum size, cost-effective, change-irreversible, and easy to read. This work presents a time-temperature sensor based on capillary imbibition of thermoplastic polymers into a mesoporous photonic crystal, which was tuned to reflect well-defined wavelengths of visible light. The photonic crystal was made by electrochemical etching of silicon, where a periodic structure was formed with microscale layers of alternating nanoscale porosity. Polymer infiltration induces an irreversible change of the effective refractive index of the crystal, leading to a progressive shift of the reflected light that can be seen by the naked eye. Importantly, the employed thermoplastic polymer (poly(ethylene vinyl-acetate)) presents a temperature-dependent viscosity that is well represented by the Arrhenius law, which is normally used to characterize the temperature-dependence of quality indexes. Therefore, each reflected color is associated to the time-temperature history of the system, representing the deterioration level of a monitored product.Fil: Cencha, Luisa Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: García, Guadalupe F.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Física del Litoral. Universidad Nacional del Litoral. Instituto de Física del Litoral; ArgentinaFil: Budini, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Física del Litoral. Universidad Nacional del Litoral. Instituto de Física del Litoral; ArgentinaFil: Urteaga, Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Física del Litoral. Universidad Nacional del Litoral. Instituto de Física del Litoral; ArgentinaFil: Berli, Claudio Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentin

Detection of quantum non-Markovianity close to the Born-Markov approximation

We calculate in an exact way the conditional past-future correlation for the decay dynamics of a two-level system in a bosonic bath. Different measurement processes are considered. In contrast to quantum memory measures based solely on system propagator properties, here memory effects are related to a convolution structure involving two system propagators and the environment correlation. This structure allows to detect memory effects even close to the validity of the Born-Markov approximation. An alternative operational-based definition of environment-to-system backflow of information follows from this result. We provide experimental support to our results by implementing the dynamics and measurements in a photonic experiment.Fil: Silva, Thais De Lima. Universidade Federal do Rio de Janeiro; BrasilFil: Walborn, Stephen P.. Universidade Federal do Rio de Janeiro; BrasilFil: Santos, Marcelo F.. Universidade Federal do Rio de Janeiro; BrasilFil: Aguilar, Gabriel H.. Universidade Federal do Rio de Janeiro; BrasilFil: Budini, Adrian Adolfo. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche; Argentina. Universidad Tecnológica Nacional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentin

Analysis of strawberry ripening by dynamic speckle measurements

This work seeks to determine the age of a fruit from observation of its dynamic speckle pattern. A mobile speckle pattern originates on the fruit's surface due to the interference of the wavefronts reflected from moving scatterers. For this work we analyzed two series of photographs of a strawberry speckle pattern, at different stages of ripening, acquired with a CMOS camera. The first day, we took ten photographs at an interval of one second. The same procedure was repeated the next day. From each series of images we extracted several statistical descriptors of pixel-to-pixel gray level variation during the observation time. By comparing these values from the first to the second day we noticed a diminution of the speckle activity. This decay demonstrated that after only one day the ripening process of the strawberry can be detected by dynamic speckle pattern analysis. For this study we employed a simple new algorithm to process the data obtained from the photographs. This algorithm allows defining a global mobility index that indicates the evolution of the fruit's ripening.Fil: Mulone, C.. Universidad Tecnológica Nacional. Facultad Regional Paraná; ArgentinaFil: Budini, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET- Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina. Universidad Tecnológica Nacional. Facultad Regional Paraná; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Santa Fe. Instituto de Física del Litoral; ArgentinaFil: Vincitorio, F. M.. Universidad Tecnológica Nacional. Facultad Regional Paraná; ArgentinaFil: Freyre, C.. Universidad Tecnológica Nacional. Facultad Regional Paraná; ArgentinaFil: López Díaz, A. J.. Universidad da Coruña; España;Fil: Ramil Rego, A.. Universidad da Coruña; España

Entropy, non-ergodicity and non-Gaussian behaviour in ballistic transport

Ballistic transportation introduces new challenges in the thermodynamic properties of a gas of particles. For example, violation of mixing, ergodicity and of the fluctuation-dissipation theorem may occur, since all these processes are connected. In this work, we obtain results for all ranges of diffusion, i.e., both for subdiffusion and superdiffusion, where the bath is such that it gives origin to a colored noise. In this way we obtain the skewness and the non-Gaussian factor for the probability distribution function of the dynamical variable. We put particular emphasis on ballistic diffusion, and we demonstrate that in this case, although the second law of thermodynamics is preserved, the entropy does not reach a maximum and a non-Gaussian behavior occurs. This implies the non-applicability of the central limit theorem.Comment: 9 pages, 2 figure

Non-equilibrium transition from dissipative quantum walk to classical random walk

We have investigated the time-evolution of a free particle in interaction with a phonon thermal bath, using the tight-binding approach. A dissipative quantum walk can be defined and many important non-equilibrium decoherence properties can be investigated analytically. The non-equilibrium statistics of a pure initial state have been studied. Our theoretical results indicate that the evolving wave-packet shows the suppression of Anderson's boundaries (ballistic peaks) by the presence of dissipation. Many important relaxation properties can be studied quantitatively, such as von Neumann's entropy and quantum purity. In addition, we have studied Wigner's function. The time-dependent behavior of the quantum entanglement between a free particle -in the lattice- and the phonon bath has been characterized analytically. This result strongly suggests the non-trivial time-dependence of the off-diagonal elements of the reduced density matrix of the system. We have established a connection between the quantum decoherence and the dissipative parameter arising from interaction with the phonon bath. The time-dependent behavior of quantum correlations has also been pointed out, showing continuous transition from quantum random walk to classical random walk, when dissipation increases.Comment: Submitted for publication. 17 pages, 6 figure

The space gamma-ray observatory AGILE

Abstract Gamma-rays of cosmic origin are a manifestation of the most energetic phenomena in our Universe. Many astrophysical sources emit gamma-rays including relativistic compact stars, massive black holes in active galactic nuclei, gamma-ray burst sources, and our Sun during intense flares. The mission AGILE ( Astro-rivelatore Gamma a Immagini LEggero ) is an innovative, cost effective gamma ray mission selected by the Italian Space Agency (ASI) as first payload of the Program for Small Scientific Missions. It is designed to detect and image gamma-ray sources in the energy range 30 MeV-50 GeV and operate as an Observatory open to the international community. Primary scientific goals include the study of AGN's, gamma ray bursts, Galactic sources, unidentified gamma ray sources, solar flares and diffuse gamma ray emission. AGILE is planned to be operational during the years 2002–2005. It will an ideal 'bridge' between EGRET and GLAST, and support space observations and ground based multiwavelength studies of high energy sources