Integration of satellite interferometric data in civil protection strategies for landslide studies at a regional scale

Multi-Temporal Satellite Interferometry (MTInSAR) is gradually evolving from being a tool developed by the scientific community exclusively for research purposes to a real operational technique that can meet the needs of different users involved in geohazard mitigation. This work aims at showing the innovative operational use of satellite radar interferometric products in Civil Protection Authority (CPA) practices for monitoring slow-moving landslides. We present the example of the successful ongoing monitoring system in the Valle D’Aosta Region (VAR-Northern Italy). This system exploits well-combined MTInSAR products and ground-based instruments for landslide management and mitigation strategies over the whole regional territory. Due to the critical intrinsic constraints of MTInSAR data, a robust regional satellite monitoring integrated into CPA practices requires the support of both in situ measurements and remotely sensed systems to guarantee the completeness and reliability of information. The monitoring network comprises three levels of analysis: Knowledge monitoring, Control monitoring, and Emergency monitoring. At the first monitoring level, MTInSAR data are used for the preliminary evaluation of the deformation scenario at a regional scale. At the second monitoring level, MTInSAR products support the prompt detection of trend variations of radar benchmarks displacements with bi-weekly temporal frequency to identify active critical situations where follow-up studies must be carried out. In the third monitoring level, MTInSAR data integrated with ground-based data are exploited to confirm active slow-moving deformations detected by on-site instruments. At this level, MTInSAR data are also used to carry out back analysis that cannot be performed by any other tool. From the example of the Valle D’Aosta Region integrated monitoring network, which is one of the few examples of this kind around Europe, it is evident that MTInSAR provides a great opportunity to improve monitoring capabilities within CPA activities

Heterotrimeric G protein subunits are located on rat liver endosomes

BACKGROUND: Rat liver endosomes contain activated insulin receptors and downstream signal transduction molecules. We undertook these studies to determine whether endosomes also contain heterotrimeric G proteins that may be involved in signal transduction from G protein-coupled receptors. RESULTS: By Western blotting G(sα), G(iα1,2), G(iα3 )and G(β )were enriched in both canalicular (CM) and basolateral (BLM) membranes but also readily detectable on three types of purified rat liver endosomes in the order recycling receptor compartment (RRC) > compartment for uncoupling of receptor and ligand (CURL) > multivesicular bodies (MVB) >> purified secondary lysosomes. Western blotting with antibodies to Na, K-ATPase and to other proteins associated with plasma membranes and intracellular organelles indicated this was not due to contamination of endosome preparations by CM or BLM. Adenylate cyclase (AC) was also identified on purified CM, BLM, RRC, CURL and MVB. Percoll gradient fractionation of liver postnuclear supernatants demonstrated co-occurrence of endosomes and heterotrimeric G protein subunits in fractions with little plasma membrane markers. By confocal microscopy, punctate staining for G(sα), G(iα3 )and G(β )corresponded to punctate areas of endocytosed Texas red-dextran in hepatocytes from control and cholera toxin-treated livers. CONCLUSION: We conclude that heterotrimeric G protein subunits as well as AC likely traffic into hepatocytes on endosome membranes, possibly generating downstream signals spatially separate from signalling generated at the plasma membrane, analogous to the role(s) of internalized insulin receptors

The Effects of Maternal and Postnatal Dietary Methyl Nutrients on Epigenetic Changes that Lead to Non-Communicable Diseases in Adulthood

The risk for non-communicable diseases in adulthood can be programmed by early nutrition. This programming is mediated by changes in expression of key genes in various metabolic pathways during development, which persist into adulthood. These developmental modifications of genes are due to epigenetic alterations in DNA methylation patterns. Recent studies have demonstrated that DNA methylation can be affected by maternal or early postnatal diets. Because methyl groups for methylation reactions come from methionine cycle nutrients (i.e., methionine, choline, betaine, folate), deficiency or supplementation of these methyl nutrients can directly change epigenetic regulation of genes permanently. Although many studies have described the early programming of adult diseases by maternal and infant nutrition, this review discusses studies that have associated early dietary methyl nutrient manipulation with direct effects on epigenetic patterns that could lead to chronic diseases in adulthood. The maternal supply of methyl nutrients during gestation and lactation can alter epigenetics, but programming effects vary depending on the timing of dietary intervention, the type of methyl nutrient manipulated, and the tissue responsible for the phenotype. Moreover, the postnatal manipulation of methyl nutrients can program epigenetics, but more research is needed on whether this approach can rescue maternally programmed offspring