Putting Soybeans into Permanent Farming

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Calibration of Boom Sprayers Using Charts to Reduce Math Calculations

The calibration of boom sprayers, although not difficult, is often neglected by producers. This article explains procedures for calibrating boom sprayers using charts and techniques rather than math calculations. It provides Extension educators with information they can use to give producers a quick and easy way to regularly calibrate their spray equipment

C-terminal residues of skeletal muscle calsequestrin are essential for calcium binding and for skeletal ryanodine receptor inhibition

BACKGROUND Skeletal muscle function depends on calcium signaling proteins in the sarcoplasmic reticulum (SR), including the calcium-binding protein calsequestrin (CSQ), the ryanodine receptor (RyR) calcium release channel, and skeletal triadin 95 kDa (trisk95) and junctin, proteins that bind to calsequestrin type 1 (CSQ1) and ryanodine receptor type 1 (RyR1). CSQ1 inhibits RyR1 and communicates store calcium load to RyR1 channels via trisk95 and/or junctin. METHODS In this manuscript, we test predictions that CSQ1's acidic C-terminus contains binding sites for trisk95 and junctin, the major calcium binding domain, and that it determines CSQ1's ability to regulate RyR1 activity. RESULTS Progressive alanine substitution of C-terminal acidic residues of CSQ1 caused a parallel reduction in the calcium binding capacity but did not significantly alter CSQ1's association with trisk95/junctin or influence its inhibition of RyR1 activity. Deletion of the final seven residues in the C-terminus significantly hampered calcium binding, significantly reduced CSQ's association with trisk95/junctin and decreased its inhibition of RyR1. Deletion of the full C-terminus further reduced calcium binding to CSQ1 altered its association with trisk95 and junctin and abolished its inhibition of RyR1. CONCLUSIONS The correlation between the number of residues mutated/deleted and binding of calcium, trisk95, and junctin suggests that binding of each depends on diffuse ionic interactions with several C-terminal residues and that these interactions may be required for CSQ1 to maintain normal muscle function.This work was supported by the Australian Research Council (DP1094219 to AFD and NAB) and a NHMRC Career Development Award (NAB)

Rickettsial pathogens and their arthropod vectors.

Rickettsial diseases, important causes of illness and death worldwide, exist primarily in endemic and enzootic foci that occasionally give rise to sporadic or seasonal outbreaks. Rickettsial pathogens are highly specialized for obligate intracellular survival in both the vertebrate host and the invertebrate vector. While studies often focus primarily on the vertebrate host, the arthropod vector is often more important in the natural maintenance of the pathogen. Consequently, coevolution of rickettsiae with arthropods is responsible for many features of the host-pathogen relationship that are unique among arthropod-borne diseases, including efficient pathogen replication, long-term maintenance of infection, and transstadial and transovarial transmission. This article examines the common features of the host-pathogen relationship and of the arthropod vectors of the typhus and spotted fever group rickettsiae

Rain: Relaxations in the sky

We demonstrate how, from the point of view of energy flow through an open system, rain is analogous to many other relaxational processes in Nature such as earthquakes. By identifying rain events as the basic entities of the phenomenon, we show that the number density of rain events per year is inversely proportional to the released water column raised to the power 1.4. This is the rain-equivalent of the Gutenberg-Richter law for earthquakes. The event durations and the waiting times between events are also characterised by scaling regions, where no typical time scale exists. The Hurst exponent of the rain intensity signal $H = 0.76 > 0.5$. It is valid in the temporal range from minutes up to the full duration of the signal of half a year. All of our findings are consistent with the concept of self-organised criticality, which refers to the tendency of slowly driven non-equilibrium systems towards a state of scale free behaviour.Comment: 9 pages, 8 figures, submitted to PR