High rate, fast timing Glass RPC for the high η\eta CMS muon detectors

The HL-LHC phase is designed to increase by an order of magnitude the amount of data to be collected by the LHC experiments. To achieve this goal in a reasonable time scale the instantaneous luminosity would also increase by an order of magnitude up to $6 \cdot 10^{34}$ cm$^{-2}$s$^{-1}$. The region of the forward muon spectrometer ($|\eta| > 1.6$) is not equipped with RPC stations. The increase of the expected particles rate up to 2 kHz/cm$^2$ ( including a safety factor 3 ) motivates the installation of RPC chambers to guarantee redundancy with the CSC chambers already present. The actual RPC technology of CMS cannot sustain the expected background level. A new generation Glass-RPC (GRPC) using low resistivity glass (LR) is proposed to equip at least the two most far away of the four high eta muon stations of CMS. The design of small size prototypes and the studies of their performances under high rate particles flux is presented.Comment: 5 pages, 5 figures, proceeding for the conference VCI 201

Effets physiologiques et metaboliques de la modulation du potentiel transmembranaire et de l'integrite membranaire electro-induits chez Escherichia coli. Application aux mecanismes de l'electrotransformation

SIGLEAvailable from INIST (FR), Document Supply Service, under shelf-number : TD 82118 / INIST-CNRS - Institut de l'Information Scientifique et TechniqueFRFranc

Les déshydratases du système FAS-II mycobactérien (régulation, rôle potentiel, impact sur la structure des acides mycoliques et sur la virulence du bacille tuberculeux)

TOULOUSE3-BU Sciences (315552104) / SudocSudocFranceF

Negative regulation by Ser/Thr phosphorylation of HadAB and HadBC dehydratases from Mycobacterium tuberculosis type II fatty acid synthase system

International audienc

Revisiting the Assignment of Rv0241c to Fatty Acid Synthase Type II of Mycobacterium tuberculosis▿

The fatty acid synthase type II enzymatic complex of Mycobacterium tuberculosis (FAS-IIMt) catalyzes an essential metabolic pathway involved in the biosynthesis of major envelope lipids, mycolic acids. The partner proteins of this singular FAS-II system represent relevant targets for antituberculous drug design. Two heterodimers of the hydratase 2 protein family, HadAB and HadBC, were shown to be involved in the (3R)-hydroxyacyl-ACP dehydration (HAD) step of FAS-IIMt cycles. Recently, an additional member of this family, Rv0241c, was proposed to have the same function, based on the heterologous complementation of a HAD mutant of the yeast mitochondrial FAS-II system. In the present work, Rv0241c was able to complement a HAD mutant in the Escherichia coli model but not a dehydratase-isomerase deficient mutant. However, an enzymatic study of the purified protein demonstrated that Rv0241c possesses a broad chain length specificity for the substrate, unlike FAS-IIMt enzymes. Most importantly, Rv0241c exhibited a strict dependence on the coenzyme A (CoA) as opposed to AcpM, the natural acyl carrier protein bearing the chains elongated by FAS-IIMt. The deletion of Rv0241c showed that this gene is not essential to M. tuberculosis survival in vitro. The resulting mutant did not display any change in the mycolic acid profile. This demonstrates that Rv0241c is a trans-2-enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydratase that does not belong to FAS-IIMt. The relevance of a heterologous complementation strategy to identifying proteins of such a system is questioned

Discovery of a novel dehydratase of the fatty acid synthase type II critical for ketomycolic acid biosynthesis and virulence of Mycobacterium tuberculosis

International audienceThe fatty acid synthase type II (FAS-II) multienzyme system builds the main chain of mycolic acids (MAs), important lipid pathogenicity factors of Mycobacterium tuberculosis (Mtb). Due to their original structure, the identification of the (3 R)-hydroxyacyl-ACP dehydratases, HadAB and HadBC, of Mtb FAS-II complex required in-depth work. Here, we report the discovery of a third dehydratase protein, HadDMtb (Rv0504c), whose gene is non-essential and sits upstream of cmaA2 encoding a cyclopropane synthase dedicated to keto- and methoxy-MAs. HadDMtb deletion triggered a marked change in Mtb keto-MA content and size distribution, deeply impacting the production of full-size molecules. Furthermore, abnormal MAs, likely generated from 3-hydroxylated intermediates, accumulated. These data strongly suggest that HadDMtb catalyzes the 3-hydroxyacyl dehydratation step of late FAS-II elongation cycles during keto-MA biosynthesis. Phenotyping of Mtb hadD deletion mutant revealed the influence of HadDMtb on the planktonic growth, colony morphology and biofilm structuration, as well as on low temperature tolerance. Importantly, HadDMtb has a strong impact on Mtb virulence in the mouse model of infection. The effects of the lack of HadDMtb observed both in vitro and in vivo designate this protein as a bona fide target for the development of novel anti-TB intervention strategies

Toward a generic and integrated FAIR data management platform

International audienceSince 2019, dataNooS (http://datanoos.univ-toulouse.fr/en/) is an academic alliance bringing together members of the academic community in Toulouse area to deal with issues related to digital resource sharing and knowledge practices. Thanks to use cases, we collect, compare and share research experiences related to the production, management and valorization of research data. We carry out a transdisciplinary process in coherence with (inter)national and disciplinary initiatives. Thanks to several experimental projects, we are currently implementing a generic and integrated platform to manage cross-disciplinary datasets. It supports dataset description with semantic metadata and their query through a faceted search interface. A prototype of the platform provides several services such as:•setting up a domain ontology adapted from a generic one to select the vocabulary for metadata descriptions;•designing templates of metadata based on this ontology, so that the quality of the metadata be checked;•a faceted search device that guides the input of search criteria thanks to the ontology;•a dataset visualization and browsing service.•repositories of digital objects (datasets, queries, templates, workflows) and knowledge (ontologies, vocabularies, catalogs, services directories);This platform is connected to the heterogeneous Open science eco-systems. Search requests are based on shared or open data repositories, metadata repositories and data portals to reference disseminated data on one point.This platform also integrates users' experience and feedback to provide personalized assistance. The platform will also support how to use the datasets in workflows, and in data processing environments.The poster presents the FAIR principles and ontologies that guided the design of the Platform. It will expose its architecture and the modules already implemented in the prototype

Toward a generic and integrated FAIR data management platform

International audienceSince 2019, dataNooS (http://datanoos.univ-toulouse.fr/en/) is an academic alliance bringing together members of the academic community in Toulouse area to deal with issues related to digital resource sharing and knowledge practices. Thanks to use cases, we collect, compare and share research experiences related to the production, management and valorization of research data. We carry out a transdisciplinary process in coherence with (inter)national and disciplinary initiatives. Thanks to several experimental projects, we are currently implementing a generic and integrated platform to manage cross-disciplinary datasets. It supports dataset description with semantic metadata and their query through a faceted search interface. A prototype of the platform provides several services such as:•setting up a domain ontology adapted from a generic one to select the vocabulary for metadata descriptions; •designing templates of metadata based on this ontology, so that the quality of the metadata be checked; •a faceted search device that guides the input of search criteria thanks to the ontology; •a dataset visualization and browsing service. •repositories of digital objects (datasets, queries, templates, workflows) and knowledge (ontologies, vocabularies, catalogs, services directories); This platform is connected to the heterogeneous Open science eco-systems. Search requests are based on shared or open data repositories, metadata repositories and data portals to reference disseminated data on one point.This platform also integrates users' experience and feedback to provide personalized assistance. The platform will also support how to use the datasets in workflows, and in data processing environments.The poster presents the FAIR principles and ontologies that guided the design of the Platform. It will expose its architecture and the modules already implemented in the prototype

Toward a generic and integrated FAIR data management platform

International audienceSince 2019, dataNooS (http://datanoos.univ-toulouse.fr/en/) is an academic alliance bringing together members of the academic community in Toulouse area to deal with issues related to digital resource sharing and knowledge practices. Thanks to use cases, we collect, compare and share research experiences related to the production, management and valorization of research data. We carry out a transdisciplinary process in coherence with (inter)national and disciplinary initiatives. Thanks to several experimental projects, we are currently implementing a generic and integrated platform to manage cross-disciplinary datasets. It supports dataset description with semantic metadata and their query through a faceted search interface. A prototype of the platform provides several services such as:•setting up a domain ontology adapted from a generic one to select the vocabulary for metadata descriptions; •designing templates of metadata based on this ontology, so that the quality of the metadata be checked; •a faceted search device that guides the input of search criteria thanks to the ontology; •a dataset visualization and browsing service. •repositories of digital objects (datasets, queries, templates, workflows) and knowledge (ontologies, vocabularies, catalogs, services directories); This platform is connected to the heterogeneous Open science eco-systems. Search requests are based on shared or open data repositories, metadata repositories and data portals to reference disseminated data on one point.This platform also integrates users' experience and feedback to provide personalized assistance. The platform will also support how to use the datasets in workflows, and in data processing environments.The poster presents the FAIR principles and ontologies that guided the design of the Platform. It will expose its architecture and the modules already implemented in the prototype