Assessment of culture and environment in the Adolescent Brain and Cognitive Development Study: Rationale, description of measures, and early data.

Neurodevelopmental maturation takes place in a social environment in addition to a neurobiological one. Characterization of social environmental factors that influence this process is therefore an essential component in developing an accurate model of adolescent brain and neurocognitive development, as well as susceptibility to change with the use of marijuana and other drugs. The creation of the Culture and Environment (CE) measurement component of the ABCD protocol was guided by this understanding. Three areas were identified by the CE Work Group as central to this process: influences relating to CE Group membership, influences created by the proximal social environment, influences stemming from social interactions. Eleven measures assess these influences, and by time of publication, will have been administered to well over 7,000 9-10 year-old children and one of their parents. Our report presents baseline data on psychometric characteristics (mean, standard deviation, range, skewness, coefficient alpha) of all measures within the battery. Effectiveness of the battery in differentiating 9-10 year olds who were classified as at higher and lower risk for marijuana use in adolescence was also evaluated. Psychometric characteristics on all measures were good to excellent; higher vs. lower risk contrasts were significant in areas where risk differentiation would be anticipated

Consolidation of Geologic Studies of Geopressured-Geothermal Resources in Texas

Two major structural styles are identified in the Wilcox growth-fault trend of the Texas Gulf Coast. The style in central and southeast Texas is characterized by continuous, closely spaced growth faults that have little associated rollover despite moderate expansion of section and that show little flattening of the fault plane with depth. Where the growth-fault trend crosses the Houston Diapir Province, growth faults are localized by preexisting salt pillows; however, the piercement salt domes formed after the main phase of faulting, so the salt tectonics "overprints" the growth faults. In South Texas (south of Live Oak County), a narrow band of growth faults having high expansion and moderate rollover lies over and downdip of a ridge of deformed, overpressured shale and lies updip of a deep Tertiary-filled basin formed by withdrawal of overpressured shale. Significant antithetic faulting is associated with this band of growth faults. Also in South Texas, the lower Wilcox Lobo trend is deformed by highly listric normal faults beneath an unconformity that is probably related to Laramide tectonic activity. Wilcox sandstone reservoirs are predominantly of high-constructive deltaic (distributary-channel and delta-front) origin. This, together with close spacing of faults and characteristically low permeabilities, limits the size of geopressured reservoirs. The largest reservoirs may be in interfault areas or in salt- or shale-withdrawal basins.Bureau of Economic Geolog

Elastic waves push organic fluids from reservoir rock

Elastic waves have been observed to increase productivity of oil wells, although the reason for the vibratory mobilization of the residual organic fluids has remained unclear. Residual oil is entrapped as ganglia in pore constrictions because of resisting capillary forces. An external pressure gradient exceeding an ‘‘unplugging’’ threshold is needed to carry the ganglia through. The vibrations help overcome this resistance by adding an oscillatory inertial forcing to the external gradient; when the vibratory forcing acts along the gradient and the threshold is exceeded, instant ‘‘unplugging’’ occurs. The mobilization effect is proportional to the amplitude and inversely proportional to the frequency of vibrations. We observe this dependence in a laboratory experiment, in which residual saturation is created in a glass micromodel, and mobilization of the dyed organic ganglia is monitored using digital photography.We also directly demonstrate the release of an entrapped ganglion by vibrations in a computational fluid-dynamics simulation

Method for Maintaining One-Dimensional Temperature Gradients in Unsaturated, Closed Soil Cells

One-dimensional temperature gradients are difficult to achieve in nonisothermal laboratory studies because, in addition to desired axial temperature gradients, ambient temperature interference (ATI) creates a radial temperature distribution. Our objective was to develop a closed soil cell with limited ATI. The cell consists of a smaller soil column, the control volume, surrounded by a larger soil column, which provides radial insulation. End boundary temperatures are controlled by a new spiral-circulation heat exchanger. Four cell size configurations were tested for ATI under varying ambient temperatures. Results indicate that cells with a 9-cm inner column diameter, 5-cm concentric soil buffer, and either 10- or 20-cm length effectively achieved one-dimensional temperature conditions. At 30°C ambient temperature, and with axial temperature gradients as large as 1°C cm−1, average steady-state radial temperature gradients in the inner soil columns were−1 Thus, these cell configurations meet the goal of maintaining a one-dimensional temperature distribution. These cells provide new opportunities for improving the study of coupled heat and water movement in soil

Targeted Metabolomics Analysis of Campylobacter coli VC167 Reveals Legionaminic Acid Derivatives as Novel Flagellar Glycans

Glycosylation of Campylobacter flagellin is required for the biogenesis of a functional flagella filament. Recently, we used a targeted metabolomics approach using mass spectrometry and NMR to identify changes in the metabolic profile of wild type and mutants in the flagellar glycosylation locus, characterize novel metabolites, and assign function to genes to define the pseudaminic acid biosynthetic pathway in Campylobacter jejuni 81-176 (McNally, D. J., Hui, J. P., Aubry, A. J., Mui, K. K., Guerry, P., Brisson, J. R., Logan, S. M., and Soo, E. C. (2006) J. Biol. Chem. 281, 18489-18498). In this study, we use a similar approach to further define the glycome and metabolomic complement of nucleotide-activated sugars in Campylobacter coli VC167. Herein we demonstrate that, in addition to CMP-pseudaminic acid, C. coli VC167 also produces two structurally distinct nucleotide-activated nonulosonate sugars that were observed as negative ions at m/z 637 and m/z 651 (CMP-315 and CMP-329). Hydrophilic interaction liquid chromatography-mass spectrometry yielded suitable amounts of the pure sugar nucleotides for NMR spectroscopy using a cold probe. Structural analysis in conjunction with molecular modeling identified the sugar moieties as acetamidino and N-methylacetimidoyl derivatives of legionaminic acid (Leg5Am7Ac and Leg5AmNMe7Ac). Targeted metabolomic analyses of isogenic mutants established a role for the ptmA-F genes and defined two new ptm genes in this locus as legionaminic acid biosynthetic enzymes. This is the first report of legionaminic acid in Campylobacter sp. and the first report of legionaminic acid derivatives as modifications on a protein

Directed evolution of a magnetic resonance imaging contrast agent for noninvasive imaging of dopamine

The development of molecular probes that allow in vivo imaging of neural signaling processes with high temporal and spatial resolution remains challenging. Here we applied directed evolution techniques to create magnetic resonance imaging (MRI) contrast agents sensitive to the neurotransmitter dopamine. The sensors were derived from the heme domain of the bacterial cytochrome P450-BM3 (BM3h). Ligand binding to a site near BM3h's paramagnetic heme iron led to a drop in MRI signal enhancement and a shift in optical absorbance. Using an absorbance-based screen, we evolved the specificity of BM3h away from its natural ligand and toward dopamine, producing sensors with dissociation constants for dopamine of 3.3–8.9 μM. These molecules were used to image depolarization-triggered neurotransmitter release from PC12 cells and in the brains of live animals. Our results demonstrate the feasibility of molecular-level functional MRI using neural activity–dependent sensors, and our protein engineering approach can be generalized to create probes for other targets.Charles A. Dana Foundation. Brain and Immuno-ImagingRaymond and Beverley Sackler FoundationNational Institutes of Health (U.S.) (grant R01-DA28299)National Institutes of Health (U.S.) (grant DP2-OD2441)National Institutes of Health (U.S.) (grant R01-GM068664)Jacobs Institute for Molecular Engineering for Medicine. Jacobs Institute for Molecular Engineering for MedicineNational Institutes of Health (U.S.) (grant R01-DE013023