Long-Term Trends of Astroclimatic Parameters above the Terskol Observatory

Astroclimatic conditions characterize the quality of an astronomical site. The Terskol Observatory was founded over 42 years ago in 1980. The astronomical site (coordinates 43°16′29″ N, 42°30′03″ E) is located about 10 km from Mt. Elbrus in the northern Caucasus Mountains. The paper presents the results of an analysis of long-term variations from 1980 to 2022 of astroclimatic parameters such as total cloud cover, precipitable water vapor, and wind speed at a level of 200 hPa above the Terskol Observatory using atmospheric ERA5 Reanalysis data from the European Center for Medium-Range Weather Forecast. The probability distributions and seasonal variations of the astroclimatic parameters are also presented. Long-term trends of the astroclimatic parameters are studied with statistically significant trend analysis methods using the Mann–Kendall test and the Sen’s slope test to estimate the magnitude of the changes. The results show non-significant decreases of annual average 200-hPa wind speed and increases in precipitable water vapor, but significant (99 per cent confidence level) long-term decreasing of total cloud cover above the Terskol Observatory

Energy Spectra of Atmospheric Turbulence for Calculating Cn2 Parameter. I. Maidanak and Suffa Observatories in Uzbekistan

Knowledge of the turbulence spectra is of interest for describing atmospheric conditions as applied to astronomical observations. This article discusses the deformations of the turbulence spectra with heights in a wide range of scales at the sites of the Maidanak and Suffa observatories. It is shown that the energy of baroclinic instability is high at the sites of these observatories and should be taken into account in the calculations of the refractive index structure constant Cn2

Energy Spectra of Atmospheric Turbulence for Calculating <inline-formula><math display="inline"><semantics><msubsup><mi>C</mi><mi>n</mi><mn>2</mn></msubsup></semantics></math></inline-formula> Parameter. I. Maidanak and Suffa Observatories in Uzbekistan

Knowledge of the turbulence spectra is of interest for describing atmospheric conditions as applied to astronomical observations. This article discusses the deformations of the turbulence spectra with heights in a wide range of scales at the sites of the Maidanak and Suffa observatories. It is shown that the energy of baroclinic instability is high at the sites of these observatories and should be taken into account in the calculations of the refractive index structure constant Cn2

Automated Recognition of Nanoparticles in Electron Microscopy Images of Nanoscale Palladium Catalysts

Automated computational analysis of nanoparticles is the key approach urgently required to achieve further progress in catalysis, the development of new nanoscale materials, and applications. Analysis of nanoscale objects on the surface relies heavily on scanning electron microscopy (SEM) as the experimental analytic method, allowing direct observation of nanoscale structures and morphology. One of the important examples of such objects is palladium on carbon catalysts, allowing access to various chemical reactions in laboratories and industry. SEM images of Pd/C catalysts show a large number of nanoparticles that are usually analyzed manually. Manual analysis of a statistically significant number of nanoparticles is a tedious and highly time-consuming task that is impossible to perform in a reasonable amount of time for practically needed large amounts of samples. This work provides a comprehensive comparison of various computer vision methods for the detection of metal nanoparticles. In addition, multiple new types of data representations were developed, and their applicability in practice was assessed

Affinity Isolation and Mass Spectrometry Identification of Prostacyclin Synthase (PTGIS) Subinteractome

Prostacyclin synthase (PTGIS; EC 5.3.99.4) catalyzes isomerization of prostaglandin H2 to prostacyclin, a potent vasodilator and inhibitor of platelet aggregation. At present, limited data exist on functional coupling and possible ways of regulating PTGIS due to insufficient information about protein&ndash;protein interactions in which this crucial enzyme is involved. The aim of this study is to isolate protein partners for PTGIS from rat tissue lysates. Using CNBr-activated Sepharose 4B with covalently immobilized PTGIS as an affinity sorbent, we confidently identified 58 unique proteins by mass spectrometry (LC-MS/MS). The participation of these proteins in lysate complex formation was characterized by SEC lysate profiling. Several potential members of the PTGIS subinteractome have been validated by surface plasmon resonance (SPR) analysis. SPR revealed that PTGIS interacted with full-length cytochrome P450 2J2 and glutathione S-transferase (GST). In addition, PTGIS was shown to bind synthetic peptides corresponding to sequences of for GSTA1, GSTM1, aldo-keto reductase (AKR1A1), glutaredoxin 3 (GLRX3) and histidine triad nucleotide binding protein 2 (HINT2). Prostacyclin synthase could potentially be involved in functional interactions with identified novel protein partners participating in iron and heme metabolism, oxidative stress, xenobiotic and drugs metabolism, glutathione and prostaglandin metabolism. The possible biological role of the recognized interaction is discussed in the context of PTGIS functioning