Tetrodotoxin-sensitive, voltage-dependent sodium currents in hair cells from the alligator cochlea

We have used whole-cell patch clamp techniques to record from tall hair cells isolated from the apical half of the alligator cochlea. Some of these cells gave action potentials in response to depolarizing current injections. When the same cells were voltage clamped, large transient inward currents followed by smaller outward currents were seen in response to depolarizing steps. We studied the transient inward current after the outward current had been blocked by external tetraethylammonium (20 mM) or by replacing internal potassium with cesium. It was found to be a sodium current because it was abolished by either replacing external sodium with choline or by external application of tetrodotoxin (100 nM). The sodium current showed voltage-dependent activation and inactivation. Most of the spiking hair cells came from the apex of the cochlea, where they would be subject to low-frequency mechanical stimulation in vivo

Timing of maternal exposure and fetal sex determine the effects of low-level chemical mixture exposure on the fetal neuroendocrine system in sheep

We have shown that continuous maternal exposure to the complex mixture of environmental chemicals (ECs) found in human biosolids (sewage sludge), disrupts mRNA expression of genes crucial for development and long-term regulation of hypothalamo-pituitary gonadal (HPG) function in sheep. This study investigated whether exposure to ECs only during preconceptional period or only during pregnancy perturbed key regulatory genes within the hypothalamus and pituitary gland and whether these effects were different from chronic (life-long) exposure to biosolid ECs. The findings demonstrate that the timing and duration of maternal EC exposure influences the subsequent effects on the fetal neuroendocrine system in a sex-specific manner. Maternal exposure prior to conception or during pregnancy only, altered the expression of key fetal neuroendocrine regulatory systems such as GnRH and kisspeptin to a greater extent than when maternal exposure was ‘life-long’. Furthermore, hypothalamic gene expression was affected to a greater extent in males than in females, and following EC exposure, male fetuses expressed more “female-like” mRNA levels for some key neuroendocrine genes. This is the first study to show that “real-life” maternal exposure to low levels of a complex cocktail of chemicals prior to conception can subsequently affect the developing fetal neuroendocrine system. These findings demonstrate that the developing neuroendocrine system is sensitive to EC mixtures in a sex-dimorphic manner likely to predispose to reproductive dysfunction in later life

Diagonalization of full finite temperature Green's function by quasi-particles

For thermal systems, standard perturbation theory breaks down because of the absence of stable, observable asymptotic states. We show, how the introduction of {\it statistical} quasi-particles (stable, but not observable) gives rise to a consistent description. Statistical and spectral information can be cleanly separated also for interacting systems.Comment: 9 pages in standard LaTe

On the Complexity of tt-Closeness Anonymization and Related Problems

An important issue in releasing individual data is to protect the sensitive information from being leaked and maliciously utilized. Famous privacy preserving principles that aim to ensure both data privacy and data integrity, such as $k$-anonymity and $l$-diversity, have been extensively studied both theoretically and empirically. Nonetheless, these widely-adopted principles are still insufficient to prevent attribute disclosure if the attacker has partial knowledge about the overall sensitive data distribution. The $t$-closeness principle has been proposed to fix this, which also has the benefit of supporting numerical sensitive attributes. However, in contrast to $k$-anonymity and $l$-diversity, the theoretical aspect of $t$-closeness has not been well investigated. We initiate the first systematic theoretical study on the $t$-closeness principle under the commonly-used attribute suppression model. We prove that for every constant $t$ such that $0\leq t<1$, it is NP-hard to find an optimal $t$-closeness generalization of a given table. The proof consists of several reductions each of which works for different values of $t$, which together cover the full range. To complement this negative result, we also provide exact and fixed-parameter algorithms. Finally, we answer some open questions regarding the complexity of $k$-anonymity and $l$-diversity left in the literature.Comment: An extended abstract to appear in DASFAA 201

Asymptotic Capture-Number and Island-Size Distributions for One-Dimensional Irreversible Submonolayer Growth

Using a set of evolution equations [J.G. Amar {\it et al}, Phys. Rev. Lett. {\bf 86}, 3092 (2001)] for the average gap-size between islands, we calculate analytically the asymptotic scaled capture-number distribution (CND) for one-dimensional irreversible submonolayer growth of point islands. The predicted asymptotic CND is in reasonably good agreement with kinetic Monte-Carlo (KMC) results and leads to a \textit{non-divergent asymptotic} scaled island-size distribution (ISD). We then show that a slight modification of our analytical form leads to an analytic expression for the asymptotic CND and a resulting asymptotic ISD which are in excellent agreement with KMC simulations. We also show that in the asymptotic limit the self-averaging property of the capture zones holds exactly while the asymptotic scaled gap distribution is equal to the scaled CND.Comment: 4 pages, 1 figure, submitted to Phys. Rev.

Testing the blast wave model with Swift GRBs

The complex structure of the light curves of Swift GRBs has made the identification of breaks, and the interpretation of the blast wave caused by the burst, more difficult than in the pre-Swift era. We aim to identify breaks, which are possibly hidden, and to constrain the blast wave parameters; electron energy distribution, p, density profile of the circumburst medium, k, and the continued energy injection index, q. We do so by comparing the observed multi-wavelength light curves and X-ray spectra of our sample to the predictions of the blast wave model. We can successfully interpret all of the bursts in our sample of 10, except two, within this framework and we can estimate, with confidence, the electron energy distribution index for 6 of the sample. Furthermore we identify jet breaks in a number of the bursts. A statistical analysis of the distribution of p reveals that, even in the most conservative case of least scatter, the values are not consistent with a single, universal value. The values of k suggest that the circumburst density profiles are not drawn from only one of the constant density or wind-like media populations.Comment: 14 pages, accepted by MNRAS after minor changes, including extension of discussion (section 4.3

On the Two Species Asymmetric Exclusion Process with Semi-Permeable Boundaries

We investigate the structure of the nonequilibrium stationary state (NESS) of a system of first and second class particles, as well as vacancies (holes), on L sites of a one-dimensional lattice in contact with first class particle reservoirs at the boundary sites; these particles can enter at site 1, when it is vacant, with rate alpha, and exit from site L with rate beta. Second class particles can neither enter nor leave the system, so the boundaries are semi-permeable. The internal dynamics are described by the usual totally asymmetric exclusion process (TASEP) with second class particles. An exact solution of the NESS was found by Arita. Here we describe two consequences of the fact that the flux of second class particles is zero. First, there exist (pinned and unpinned) fat shocks which determine the general structure of the phase diagram and of the local measures; the latter describe the microscopic structure of the system at different macroscopic points (in the limit L going to infinity in terms of superpositions of extremal measures of the infinite system. Second, the distribution of second class particles is given by an equilibrium ensemble in fixed volume, or equivalently but more simply by a pressure ensemble, in which the pair potential between neighboring particles grows logarithmically with distance. We also point out an unexpected feature in the microscopic structure of the NESS for finite L: if there are n second class particles in the system then the distribution of first class particles (respectively holes) on the first (respectively last) n sites is exchangeable.Comment: 28 pages, 4 figures. Changed title and introduction for clarity, added reference

Pattern matching and pattern discovery algorithms for protein topologies

We describe algorithms for pattern matching and pattern learning in TOPS diagrams (formal descriptions of protein topologies). These problems can be reduced to checking for subgraph isomorphism and finding maximal common subgraphs in a restricted class of ordered graphs. We have developed a subgraph isomorphism algorithm for ordered graphs, which performs well on the given set of data. The maximal common subgraph problem then is solved by repeated subgraph extension and checking for isomorphisms. Despite the apparent inefficiency such approach gives an algorithm with time complexity proportional to the number of graphs in the input set and is still practical on the given set of data. As a result we obtain fast methods which can be used for building a database of protein topological motifs, and for the comparison of a given protein of known secondary structure against a motif database

Using the stated preference method for the calculation of social discount rate

The aim of this paper is to build the stated preference method into the social discount rate methodology. The first part of the paper presents the results of a survey about stated time preferences through pair-choice decision situations for various topics and time horizons. It is assumed that stated time preferences differ from calculated time preferences and that the extent of stated rates depends on the time period, and on how much respondents are financially and emotionally involved in the transactions. A significant question remains: how can the gap between the calculation and the results of surveys be resolved, and how can the real time preferences of individuals be interpreted using a social time preference rate. The second part of the paper estimates the social time preference rate for Hungary using the results of the survey, while paying special attention to the pure time preference component. The results suggest that the current method of calculation of the pure time preference rate does not reflect the real attitudes of individuals towards future generations