'The effect of different genres of music on the stress levels of kennelled dogs'

Classical music has been shown to reduce stress in kennelled dogs; however, rapid habituation of dogs to this form of auditory enrichment has also been demonstrated. The current study investigated the physiological and behavioural response of kennelled dogs (n = 38) to medium-term (5 days) auditory enrichment with five different genres of music including Soft Rock, Motown, Pop, Reggae and Classical, to determine whether increasing the variety of auditory stimulation reduces the level of habituation to auditory enrichment. Dogs were found to spend significantly more time lying and significantly less time standing when music was played, regardless of genre. There was no observable effect of music on barking, however, dogs were significantly (z = 2.2, P < 0.05) more likely to bark following cessation of auditory enrichment. Heart Rate Variability (HRV) was significantly higher, indicative of decreased stress, when dogs were played Soft Rock and Reggae, with a lesser effect observed when Motown, Pop and Classical genres were played. Relative to the silent period prior to auditory enrichment, urinary cortisol:creatanine (UCCR) values were significantly higher during Soft Rock (t = 2.781, P < 0.01) and the second silent control period following auditory enrichment (t = 2.46, P < 0.05). Despite the mixed response to different genres, the physiological and behavioural changes observed remained constant over the 5d of enrichment suggesting that the effect of habituation may be reduced by increasing the variety of auditory enrichment provided

A reduction in long-term spatial memory persists after discontinuation of peripubertal GnRH agonist treatment in sheep

Chronic gonadotropin-releasing hormone agonist (GnRHa) administration is used where suppression of hypothalamic-pituitary-gonadal axis activity is beneficial, such as steroid-dependent cancers, early onset gender dysphoria, central precocious puberty and as a reversible contraceptive in veterinary medicine. GnRH receptors, however, are expressed outside the reproductive axis, e.g. brain areas such as the hippocampus which is crucial for learning and memory processes. Previous work, using an ovine model, has demonstrated that long-term spatial memory is reduced in adult rams (45 weeks of age), following peripubertal blockade of GnRH signaling (GnRHa: goserelin acetate), and this was independent of the associated loss of gonadal steroid signaling. The current study investigated whether this effect is reversed after discontinuation of GnRHa-treatment. The results demonstrate that peripubertal GnRHa-treatment suppressed reproductive function in rams, which was restored after cessation of GnRHa-treatment at 44 weeks of age, as indicated by similar testes size (relative to body weight) in both GnRHa-Recovery and Control rams at 81 weeks of age. Rams in which GnRHa-treatment was discontinued (GnRHa-Recovery) had comparable spatial maze traverse times to Controls, during spatial orientation and learning assessments at 85 and 99 weeks of age. Former GnRHa-treatment altered how quickly the rams progressed beyond a specific point in the spatial maze at 83 and 99 weeks of age, and the direction of this effect depended on gonadal steroid exposure, i.e. GnRHa-Recovery rams progressed quicker during breeding season and slower during non-breeding season, compared to Controls. The long-term spatial memory performance of GnRHa-Recovery rams remained reduced (P < 0.05, 1.5-fold slower) after discontinuation of GnRHa, compared to Controls. This result suggests that the time at which puberty normally occurs may represent a critical period of hippocampal plasticity. Perturbing normal hippocampal formation in this peripubertal period may also have long lasting effects on other brain areas and aspects of cognitive function

Spatial memory is impaired by peripubertal GnRH agonist treatment and testosterone replacement in sheep

Chronic gonadotropin-releasing hormone agonist (GnRHa) is used therapeutically to block activity within the reproductive axis through down-regulation of GnRH receptors within the pituitary gland. GnRH receptors are also expressed in non-reproductive tissues, including areas of the brain such as the hippocampus and amygdala. The impact of long-term GnRHa-treatment on hippocampus-dependent cognitive functions, such as spatial orientation, learning and memory, is not well studied, particularly when treatment encompasses a critical window of development such as puberty. The current study used an ovine model to assess spatial maze performance and memory of rams that were untreated (Controls), had both GnRH and testosterone signaling blocked (GnRHa-treated), or specifically had GnRH signaling blocked (GnRHa-treated with testosterone replacement) during the peripubertal period (8, 27 and 41 weeks of age). The results demonstrate that emotional reactivity during spatial tasks was compromised by the blockade of gonadal steroid signaling, as seen by the restorative effects of testosterone replacement, while traverse times remained unchanged during assessment of spatial orientation and learning. The blockade of GnRH signaling alone was associated with impaired retention of long-term spatial memory and this effect was not restored with the replacement of testosterone signaling. These results indicate that GnRH signaling is involved in the retention and recollection of spatial information, potentially via alterations to spatial reference memory, and that therapeutic medical treatments using chronic GnRHa may have effects on this aspect of cognitive function

Timing of maternal exposure and fetal sex determine the effects of low-level chemical mixture exposure on the fetal neuroendocrine system in sheep

We have shown that continuous maternal exposure to the complex mixture of environmental chemicals (ECs) found in human biosolids (sewage sludge), disrupts mRNA expression of genes crucial for development and long-term regulation of hypothalamo-pituitary gonadal (HPG) function in sheep. This study investigated whether exposure to ECs only during preconceptional period or only during pregnancy perturbed key regulatory genes within the hypothalamus and pituitary gland and whether these effects were different from chronic (life-long) exposure to biosolid ECs. The findings demonstrate that the timing and duration of maternal EC exposure influences the subsequent effects on the fetal neuroendocrine system in a sex-specific manner. Maternal exposure prior to conception or during pregnancy only, altered the expression of key fetal neuroendocrine regulatory systems such as GnRH and kisspeptin to a greater extent than when maternal exposure was ‘life-long’. Furthermore, hypothalamic gene expression was affected to a greater extent in males than in females, and following EC exposure, male fetuses expressed more “female-like” mRNA levels for some key neuroendocrine genes. This is the first study to show that “real-life” maternal exposure to low levels of a complex cocktail of chemicals prior to conception can subsequently affect the developing fetal neuroendocrine system. These findings demonstrate that the developing neuroendocrine system is sensitive to EC mixtures in a sex-dimorphic manner likely to predispose to reproductive dysfunction in later life

Real time thermal propagtors for massive gauge bosons

We derive Feynman rules for gauge theories exhibiting spontaneous symmetry breaking using the real-time formalism of finite temperature field theory. We also derive the thermal propagators where only the physical degrees of freedom are given thermal boundary conditions. We analyse the abelian Higgs model and find that these new propagators simplify the calculation of the thermal contribution to the self energy.Comment: 7 pages, late

Buried solutions:How Maya urban life substantiates soil connectivity

Soils are a pivot of sustainable development. Yet, urban planning decisions persist in compromising the usability of the urban soils resource. Urban land cover expansion to accommodate an increasing population results in soil sealing. Concealment of and physical obstructions to soils prevent urban populations from engaging with their soil dependency. The concept of soil connectivity recognises that nurturing mutually beneficial soil–society relations is an essential dimension for achieving soil security. The concentrated populations of urban environments acutely require productive soil–society relations and offer the greatest potential for enhancing soil connectivity. Soil connectivity remains notably under-researched, however, resulting in deficient evidence to substantiate exactly how soil connectivity can contribute to sustaining urban life. The entanglement of soil and urban development has been critical throughout history, but seldom recognised in soil security discourse. We review the manifestation of effective soil connectivity in Precolumbian lowland Maya tropical urbanism. Archaeological evidence reveals, first, that lowland Maya urban settlement patterns largely preserved the availability, proximity, and accessibility of soils in the subdivision and configuration of urban open space. Second, Maya urban life included practices that proactively contributed to the formation of soils by adding to the stock of soils and improving beneficial soil properties of the thin and often nutrient-poor soils resulting from the regionally dominant karstic lithology. Third, a range of Maya landscape modifications and engineering practices enabled the preservation and protection of soils within urban environments. We derive evidence-based insights on an urban tradition that endured for well over two millennia by incorporating intensive soil–society relationships to substantiate the concept of soil connectivity. Inspiring urban planning to stimulate soil connectivity through enhancing the engagement with soils in urban life would promote soil security

Separated magnet yoke for permanent magnet linear generator for marine wave energy converters

In this paper the performance of a longitudinal flux permanent magnet linear generator (PMLG) for wave energy converters (WEC) is investigated. The influence of the number of slots per pole, phase q and the number of stator's winding sections are analysed. The power output and the cogging forces in the PMLG are calculated and reviewed with respect to the above design parameters. In addition, an optimised PMLG model is designed and simulated. Three-dimensional Finite Element Method (FEM) is used for solving the combined field and circuit equations of the generator.The PRIMaRE project

Supersymmetry in Boundary Integrable Models

Quantum integrable models that possess $N=2$ supersymmetry are investigated on the half-space. Conformal perturbation theory is used to identify some $N=2$ supersymmetric boundary integrable models, and the effective boundary Landau-Ginzburg formulations are constructed. It is found that $N=2$ supersymmetry largely determines the boundary action in terms of the bulk, and in particular, the boundary bosonic potential is $|W|^2$, where $W$ is the bulk superpotential. Supersymmetry is also investigated using the affine quantum group symmetry of exact scattering matrices, and the affine quantum group symmetry of boundary reflection matrices is analyzed both for supersymmetric and more general models. Some $N=2$ supersymmetry preserving boundary reflection matrices are given, and their connection with the boundary Landau-Ginzburg actions is discussed.Comment: 37 pages and two figures; lanlmac or harvmac, eps

Biomarkers of oxidative stress: methods and measures of oxidative DNA damage (COMET assay) and telomere shortening

Oxidative stress is fast becoming the nutritional and medical buzzword for the twenty-first century. The theoretical importance of oxidative stress in diabetes is highlighted by its potential double impact on metabolic dysfunction on one hand and the vascular system on the other hand. The new concept of oxidative stress, being an important trigger in the onset and progression of diabetes and its complications, emphasizes the need for measurement of markers of oxidation to assess the degree of oxidative stress. While we have been routinely measuring biomarkers in our molecular epidemiology projects, here we discuss the utility of two assays, (a) DNA damage assessment by COMET measurement and (b) telomere length measurement. As DNA damage is efficiently repaired by cellular enzymes, its measurement gives a snapshot view of the level of oxidative stress. The protocol allows for measurement of oxidative DNA damage (FPG-sensitive DNA strand breaks). Telomere length measured by Southern blotting technique allows one to estimate the chronic burden of oxidative stress at the molecular level and is now considered as biomarker of biological aging

Identity of the imaginary-time and real-time thermal propagators for scalar bound states in a one-generation Nambu-Jona-Lasinio model

By rigorous reanalysis of the results, we have proven that the propagators at finite temperature for scalar bound states in one-generation fermion condensate scheme of electroweak symmetry breaking are in fact identical in the imaginary-time and the real-time formalism. This dismisses the doubt about possible discrepancy between the two formalisms in this problem. Identity of the derived thermal transformation matrices of the real-time matrix propagators for scalar bound states without and with chemical potential and the ones for corresponding elementary scalar particles shows similarity of thermodynamic property between the two types of particles. Only one former inference is modified, i.e. when the two flavors of fermions have unequal nonzero masses, the amplitude of the composite Higgs particle will decay instead grow in time.Comment: 5 pages, revtex4, no figure