Why the short face? Developmental disintegration of the neurocranium drives convergent evolution in neotropical electric fishes

© 2017 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. Convergent evolution is widely viewed as strong evidence for the influence of natural selection on the origin of phenotypic design. However, the emerging evo-devo synthesis has highlighted other processes that may bias and direct phenotypic evolution in the presence of environmental and genetic variation. Developmental biases on the production of phenotypic variation may channel the evolution of convergent forms by limiting the range of phenotypes produced during ontogeny. Here, we study the evolution and convergence of brachycephalic and dolichocephalic skull shapes among 133 species of Neotropical electric fishes (Gymnotiformes: Teleostei) and identify potential developmental biases on phenotypic evolution. We plot the ontogenetic trajectories of neurocranial phenotypes in 17 species and document developmental modularity between the face and braincase regions of the skull. We recover a significant relationship between developmental covariation and relative skull length and a significant relationship between developmental covariation and ontogenetic disparity. We demonstrate that modularity and integration bias the production of phenotypes along the brachycephalic and dolichocephalic skull axis and contribute to multiple, independent evolutionary transformations to highly brachycephalic and dolichocephalic skull morphologies

Necrotic myocardial cells release damage-associated molecular patterns that provoke fibroblast activation in vitro and trigger myocardial inflammation and fibrosis in vivo

BACKGROUND: Tissue injury triggers inflammatory responses that promote tissue fibrosis; however, the mechanisms that couple tissue injury, inflammation, and fibroblast activation are not known. Given that dying cells release proinflammatory “damage-associated molecular patterns” (DAMPs), we asked whether proteins released by necrotic myocardial cells (NMCs) were sufficient to activate fibroblasts in vitro by examining fibroblast activation after stimulation with proteins released by necrotic myocardial tissue, as well as in vivo by injecting proteins released by necrotic myocardial tissue into the hearts of mice and determining the extent of myocardial inflammation and fibrosis at 72 hours. METHODS AND RESULTS: The freeze–thaw technique was used to induce myocardial necrosis in freshly excised mouse hearts. Supernatants from NMCs contained multiple DAMPs, including high mobility group box-1 (HMGB1), galectin-3, S100β, S100A8, S100A9, and interleukin-1α. NMCs provoked a significant increase in fibroblast proliferation, α–smooth muscle actin activation, and collagen 1A1 and 3A1 mRNA expression and significantly increased fibroblast motility in a cell-wounding assay in a Toll-like receptor 4 (TLR4)- and receptor for advanced glycation end products–dependent manner. NMC stimulation resulted in a significant 3- to 4-fold activation of Akt and Erk, whereas pretreatment with Akt (A6730) and Erk (U0126) inhibitors decreased NMC-induced fibroblast proliferation dose-dependently. The effects of NMCs on cell proliferation and collagen gene expression were mimicked by several recombinant DAMPs, including HMGB1 and galectin-3. Moreover, immunodepletion of HMGB1 in NMC supernatants abrogated NMC-induced cell proliferation. Finally, injection of NMC supernatants or recombinant HMGB1 into the heart provoked increased myocardial inflammation and fibrosis in wild-type mice but not in TLR4-deficient mice. CONCLUSIONS: These studies constitute the initial demonstration that DAMPs released by NMCs induce fibroblast activation in vitro, as well as myocardial inflammation and fibrosis in vivo, at least in part, through TLR4-dependent signaling

How to tuna fish:constraint, convergence, and integration in the neurocranium of pelagiarian fishes

Morphological evolution of the vertebrate skull has been explored across a wide range of tetrapod clades using geometric morphometrics, but the application of these methods to teleost fishes, accounting for roughly half of all vertebrate species, has been limited. Here we present the results of a study investigating 3D morphological evolution of the neurocranium across 114 species of Pelagiaria, a diverse clade of open-ocean teleost fishes that includes tuna and mackerel. Despite showing high shape disparity overall, taxa from all families fall into three distinct morphological clusters. Convergence in shape within clusters is high, and phylogenetic signal in shape data is significant but low. Neurocranium shape is significantly correlated with body elongation and significantly but weakly correlated with size. Diet and habitat depth are weakly correlated with shape, and nonsignificant after accounting for phylogeny. Evolutionary integration in the neurocranium is high, suggesting that convergence in skull shape and the evolution of extreme morphologies are associated with the correlated evolution of neurocranial elements. These results suggest that shape evolution in the pelagiarian neurocranium reflects the extremes in elongation found in body shape but is constrained along relatively few axes of variation, resulting in repeated evolution toward a restricted range of morphologies.</p

Ecological and Economic Effects of Derelict Fishing Gear in the Chesapeake Bay 2015/2016 Final Assessment Report

Derelict fishing gear represents a major challenge to marine resource management: whether through deliberate abandonment or through accidental loss, derelict traps in particular have significant negative effects both economic (e.g., reduced fishery harvest from ghost fishing and gear competition that leads to the reduced efficiency of active gear) and ecological (e.g., degraded habitats and marine food webs and crab and bycatch mortality). Throughout the Chesapeake Bay, commercial harvest of hard-shelled blue crabs is a major fishing activity: every year sees the deployment of several hundred thousand blue crab traps (known locally as crab “pots”) across the Bay, of which an estimated 12-20% are lost each year. This report focuses on these derelict crab pots, drawing on many direct or remote observations and other data to quantify their abundance and spatial distribution across the Chesapeake Bay, and their resulting ecological and economic effects

Fluctuations in Evolutionary Integration Allow for Big Brains and Disparate Faces

In theory, evolutionary modularity allows anatomical structures to respond differently to selective regimes, thus promoting morphological diversification. These differences can then influence the rate and direction of phenotypic evolution among structures. Here we use geometric morphometrics and phenotypic matrix statistics to compare rates of craniofacial evolution and estimate evolvability in the face and braincase modules of a clade of teleost fishes (Gymnotiformes) and a clade of mammals (Carnivora), both of which exhibit substantial craniofacial diversity. We find that the face and braincase regions of both clades display different degrees of integration. We find that the face and braincase evolve at similar rates in Gymnotiformes and the reverse in Carnivora with the braincase evolving twice as fast as the face. Estimates of evolvability and constraints in these modules suggest differential responses to selection arising from fluctuations in phylogenetic integration, thus influencing differential rates of skull-shape evolution in these two clades

Effects of eight neuropsychiatric copy number variants on human brain structure

Many copy number variants (CNVs) confer risk for the same range of neurodevelopmental symptoms and psychiatric conditions including autism and schizophrenia. Yet, to date neuroimaging studies have typically been carried out one mutation at a time, showing that CNVs have large effects on brain anatomy. Here, we aimed to characterize and quantify the distinct brain morphometry effects and latent dimensions across 8 neuropsychiatric CNVs. We analyzed T1-weighted MRI data from clinically and non-clinically ascertained CNV carriers (deletion/duplication) at the 1q21.1 (n = 39/28), 16p11.2 (n = 87/78), 22q11.2 (n = 75/30), and 15q11.2 (n = 72/76) loci as well as 1296 non-carriers (controls). Case-control contrasts of all examined genomic loci demonstrated effects on brain anatomy, with deletions and duplications showing mirror effects at the global and regional levels. Although CNVs mainly showed distinct brain patterns, principal component analysis (PCA) loaded subsets of CNVs on two latent brain dimensions, which explained 32 and 29% of the variance of the 8 Cohen’s d maps. The cingulate gyrus, insula, supplementary motor cortex, and cerebellum were identified by PCA and multi-view pattern learning as top regions contributing to latent dimension shared across subsets of CNVs. The large proportion of distinct CNV effects on brain morphology may explain the small neuroimaging effect sizes reported in polygenic psychiatric conditions. Nevertheless, latent gene brain morphology dimensions will help subgroup the rapidly expanding landscape of neuropsychiatric variants and dissect the heterogeneity of idiopathic conditions

Evolution of Craniofacial Diversity in Neotropical Electric Fishes (Gymnotiformes: Teleostei)

Herein I study patterns of variation and their relationship to patterns of diversity in Neotropical electric fishes (Gymnotiformes: Teleostei). In Chapter I, I performed a taxonomic revision on a clade of deep channel electric-fishes (Sternarchella). In this revision, I described two new species and resolved taxonomic confusion about species identity in other taxa. In Chapter II, I performed a geometric morphometric analysis of gymnotiform skull shape. I integrated shape data with phylogenetic data to quantify the degree of convergent evolution within this clade. I found more instances of independent evolution towards short-snouted phenotypes than long-snouted phenotypes, and described a novel convergent phenotype. After identifying convergent craniofacial phenotypes, I tracked the neurocranial development and developmental integration of 17 species (363 specimens) that exhibited various convergent phenotypes. I found that the developmental trajectories were highly irregular for short-snouted species, while the long-snouted species exhibited more regular and consistent developmental patterns. I also found a significant relationship between developmental integration and adult skull shape. In Chapter III, I tracked the evolutionary integration of the face and braincase modules of the skull. I compared the gymnotiform and carnivoran patterns of neurocranial evolution and quantified the skulls’ ability to respond to selection pressures. I found that the carnivoran skull is modular and that this modularity allowed the braincase to exhibit significantly higher degrees of respondability to selection allowing it to evolve twice as fast as the face. In Gymnotiformes, I found that face and braincase were highly integrated and exhibited similar magnitudes of response to selection. This pattern of integration allowed for the similar rates of face and braincase shape evolution in this clade. In Chapter IV, I studied the trophic ecology of seven electric fish species that exhibit two types of brachycephalic skulls shapes, using stable isotopes and gut-content analyses to study the effect of developmental biases on the evolution of adaptive phenotypes. Using these data, I described four trophic guilds that did not cluster within the two skull shapes. This diversity in trophic ecologies associated with the conserved brachycephalic phenotypes suggests that the brachycephalic skull is widely adaptable for a variety of functions

Taxonomic Revision Of The Deep Channel Electric Fish Genus Sternarchella (Teleostei: Gymnotiformes: Apteronotidae), With Descriptions Of Two New Species

This paper provides a taxonomic revision of the Neotropical electric fish genus Sternarchella, with redescriptions of seven valid species and descriptions of two new species. A maximum parsimony analysis of 76 morphological characters from seven ingroup and seven outgroup taxa recovered a non-monophyletic Sternarchella, in which a clade comprising two species with a ventral mouth (S. orinoco + S. sima) is the sister group to a clade comprising seven species that possess a terminal or superior mouth. Nested within this higher-diversity clade is the genus Magosternarchus (recognized herein as a junior synonym of Sternarchella) comprising M. duccis and M. raptor. The Magosternarchus clade forms a polytomy with S. orthos and S. schotti. Sternarchella calhamazon + a new species from the upper Río Madeira (sister species to S. calhamazon), and a new larger-bodied species from the central and upper Río Amazonas also form a clade. Sternarchella orthos is distributed in both the Amazon and Orinoco basins, where it exhibits considerable phenotypic diversity. Sternarchella orthos includes most specimens from the Amazon formerly assigned to the nominal species S. terminalis (recognized herein as a junior synonym of S. schotti)

Taxonomic revision of the deep channel electric fish genus Sternarchella (Teleostei: Gymnotiformes: Apteronotidae), with descriptions of two new species

ABSTRACT This paper provides a taxonomic revision of the Neotropical electric fish genus Sternarchella, with redescriptions of seven valid species and descriptions of two new species. A maximum parsimony analysis of 76 morphological characters from seven ingroup and seven outgroup taxa recovered a non-monophyletic Sternarchella, in which a clade comprising two species with a ventral mouth (S. orinoco + S. sima) is the sister group to a clade comprising seven species that possess a terminal or superior mouth. Nested within this higher-diversity clade is the genus Magosternarchus (recognized herein as a junior synonym of Sternarchella) comprising M. duccis and M. raptor. The Magosternarchus clade forms a polytomy with S. orthos and S. schotti. Sternarchella calhamazon + a new species from the upper Río Madeira (sister species to S. calhamazon), and a new larger-bodied species from the central and upper Río Amazonas also form a clade. Sternarchella orthos is distributed in both the Amazon and Orinoco basins, where it exhibits considerable phenotypic diversity. Sternarchella orthos includes most specimens from the Amazon formerly assigned to the nominal species S. terminalis (recognized herein as a junior synonym of S. schotti)

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