酵素の作用(退官記念最終講義)

Contains sequential images of Yale logo experimental treatment, replicate

Yale Y Replicate 1

Contains sequential images of Yale logo experimental treatment, replicate

Grid_700_Replicate 1_(exp1_5)

Contains sequential images of replicate 1 of the grid with intersections spaced every 700 micron

Data from: Control of hydroid colony form by surface heterogeneity

The colonial hydroid Podocoryna carnea grows adherent to surfaces progressing along them by a motile stolon tip. We here ask whether the stolon tip grows preferentially within grooves etched in silicon wafers. In a series of pilot experiments, we varied the dimensions of grooves and found that stolons did not utilize grooves with a width:depth of 5:5 μm or 10:10 μm, occasionally followed grooves 25:25 μm in size, and preferentially grew within grooves of a width:depth of 50:50 μm and 100:50 μm. We then grew colonies in grids, with fixed 50:50 μm width:depth channels intersecting at 90° every 950, 700, 450, or 150 μm. We find that stolons grew within grooves early in colony ontogeny, but remained restricted to them only in the grid pattern with channel intersections every 150 μm. Finally, we created a grid in the shape of the Yale Y logo, with channels of 50:50 μm width:depth and intersections every 100 μm. The resulting colonies conformed to that of the logo. Our findings demonstrate that stolons respond to surface heterogeneity and that surface etching can be used to fabricate microfluidic circuits comprised of hydroid perisarc

B2_Replicate Mid-Ontogeny_2 Jul

Digitized images documenting colony morphology for replicate films. Scale bar = 1mm. Blue open circle corresponds to size and location of fed polyp. Red open circles correspond to size and locations of unfed stolons

A4 Late ontogeny_12 June_Short

Late ontogeny colony_12 June 2014_Short 1.3 First image at 16 minutes post-feeding, subsequent images at 10 sec intervals. Exposure = 0.5 sec. The short exposure was used to quantify the luminosity of the fed polyp. Scale: 5.97 µm/pixel

B5_Replicate Stolon Growth_20 Jun

Digitized images documenting colony morphology for replicate films. Scale bar = 1mm. Blue open circle corresponds to size and location of fed polyp. Red open circles correspond to size and locations of unfed stolons

La Petite presse : journal quotidien... / [rédacteur en chef : Balathier Bragelonne]

04 décembre 18671867/12/04 (A2,N594)

Grid_450_Replicate 2 (exp2_6)

Contains sequential images of replicate 2 of the grid with intersections spaced every 450 microns

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