A Kinesin Driven Enzyme Linked Immunosorbant Assay (ELISA) for Ultra Low Protein Detection Applications

<p>Gene Ontology bar charts for three different criteria: biological process (A), cell localization (B) and molecular function (C). The list of differentially expressed genes after RSV diet in neocortex were classified by Gene Ontology (GO) and drawn using Panther (<a href="https://www.pantherdb.org/" target="_blank">www.pantherdb.org/</a>)</p

Immunohistochemistry for Apo D and amount of Apo D protein in some nuclei along different decades.

<p>A, B) Dorsal motor nucleus of the vagus (DX). C,D) Nucleus of solitary tractis (NST). E, F) Inferior olivary nucleus (IO). Data presented as means of relative optical density (ROD). Each point in the graph represents mean density in a x20 field ±standar error of the mean. Regresion lines and Pearson`s correlation coefficient (r) are also shown. Bar: 40 μm.</p

Immunohistochemistry for Apo D and amount of Apo D protein in vestibular nuclear complex along different decades.

<p>A, B) Inferior vestibular nucleus (IVN). C,D) Medial vestibular nucleus (MVN). E, F) Lateral vestibular nucleus (LVN). G, H) Superior vestibular nucleus (SVN). Data presented as means of relative optical density (ROD). Each point in the graph represents mean density in a x20 field ±standar error of the mean. Regresion lines and Pearson`s correlation coefficient (r) are also shown. Bar: 40 μm.</p

Immunohistochemistry for Apo D and amount of Apo D protein in some nuclei along different decades.

<p>A, B) Hypoglossal nucleus (XII). C,D) Oculomotor nucleus (III). E, F) Facial motor nucleus (VII). Roller`s nucleus (RN). Data presented as means of relative optical density (ROD). Each point in the graph represents mean density in a x20 field ±standar error of the mean. Regresion lines and Pearson`s correlation coefficient (r) are also shown. Bar: 40 μm.</p

Mean of Apo D protein during aging in each nuclei of medulla oblongata studied.

<p>Nuclei are grouped according their sensory or motor function. Motor nuclei show higher quantity of Apo D than sensory nuclei. Data presented as means of relative optical density (ROD). Bars represent mean value of Apo D along all decades.</p

Hemisections of medulla oblongata.

<p>Four levels of medulla oblongata (A,B,C,D), stained with a Nissl/Myelin technique, with the localization of the different nuclei studied in the present paper are showed. </p

Slot blot analysis and amount of Apo D protein along different ages and decades.

<p>A) Samples from medulla oblongata of different subjects during aging. B) Amount of Apo D in samples from subjects between 35 and 82 years old. The graphic shows the densitometric analysis of slot blots. Data presented as means of relative optical density (ROD). Each point in the graph represents mean density ±standar error of the mean. Regresion lines and Pearson`s correlation coefficient (r) is also shown.</p

Number of differentially expressed genes detected by GeneSpring depending on p-value and absolute fold change (FC) values.

<p>Analysis of data, from control- and RSV-enriched diet both in quintuplicates, were performed using moderated T-test, the multiple testing correction of Benjamini-Hochberg and asymptotic p-value (adjusted) computation.</p

Gene expression evaluated by reverse transcription, quantitative real-time PCR (RT-qPCR) in control and in 100 μM RSV-treated C6 glioma cells.

<p>mRNA samples isolated from cells incubated for 24 h were analyzed by RT-qPCR using specific probes for <i>Atp1b2</i>, <i>Vamp2</i>, <i>Rab2a</i>, and <i>Dnm1</i>. β-actin mRNA was used as control. Data are mean ± SEM of four independent experiments. * p < 0.05 according to Student’s t test.</p

Selected differentially expressed genes.

<p>Selected differentially expressed genes.</p