Pengaruh Jamur Gliocladium SP. dan Bakteri Pseudomonas Fluorencens dalam Menekan Perkembangan Penyakit Layu Fusarium pada Tanaman Pisang Mas (Musa Paradisiaca L.) Hasil Kultur Invitro

The objective of the study is to determine the potence of natural agent, a mmushroom of Gliocladium sp. and bacterium Peudomonas fluorencens in resisting against the withering disease (Fusarium Oxysporum f.sp. Cubense) and their effect to the growth of the in-vitrocultured banana plant (Musa paradisiaca L). Thiswas conducted in the experimental farm of Agriculture Faculty, University of Muhammadiyah Purwokerto, in the period of June to December 2013.This research is a single experiment using Randomized Completely Block Design. The treatment was the administration of Gliocladium sp. In three different doses of 10 g/polybag (G1),20 g/ polybag (G2), 30 g/ polybag (G3), and the giving of Pseudomonas fluorencens inthree different dosage of10 ml/l water/ polybag (PF1),20 ml/l water/polybag (PF2) and 30 ml/l water/polybag(PF3) and one control group of no treatment (K0).Based on the result of data analysis, it is proved that the treatment of natural agents of Gliocladiumsp and Pseudomonas fluorencenshas induced the plants resistance against the withering disease of FusariumOxysporumf.sp. Cubense in the banana, as it is indicated bythe increase of phenol compounds, i.e. glychoseda, saponin, and thanin. However, the treatment has no significant effect on the plant growth either on their leaves or their stalk diameter

Pengaruh Asupan Protein terhadap Kejadian Hipertensi pada Wanita Usia Subur dengan Obesitas di Puskesmas Patumbak Tahun 2017

Hipertensi dapat diakibatkan karena kondisi tubuh orang yang gemuk, Obesitas dan hipertensi adalah masalah kesehatan yang ada di masyarakat Indonesia bahkan dikalangan wanita usia subur. Tujuan penelitian: untuk mengetahui asupan protein dan asupan garam terhadap kejadian Hipertensi pada WUS obesitas di Patumbak. Metode penelitian case control. Sampel dalam penelitian ini adalah 104 WUS (52 kasus dan 52 kontrol). Pengambilan data menggunakan penimbangan berat badan, tinggi badan, tekanan darah dan wawancara recall konsumsi. Analisis bivariate (regresi logistik sederhana) asupan protein (0,003), ada pengaruh signifikan antara asupan protein. Saran: WUS agar mengontrol berat badan dan memeriksakan tekanan darah secara teratur dan mengikuti edukasi tentang pola makan

3-[3-(4-Bromo­phen­yl)-1-phenyl-1H-pyrazol-4-yl]-5-eth­oxy-2-phenyl­isoxazolidine

In the title compound, C26H24BrN3O2, the isoxazolidine ring adopts an envelope conformation, the ring N atom deviating from the mean plane of the other four atoms by an angle of 0.286°. The orientation of the phenyl ring is +sp and the bromophenyl ring is +sc relative to the attached pyrazole ring; the dihedral angles between the least-squares planes of the pyrazole and the attached phenyl and bromophenyl rings are 21.8 (3) and 41.8 (3)°

6′-Amino-3′-methyl-2-oxo-1′-phenyl-1′,3a′,4′,7a′-tetra­hydrospiro­[1H-indole-3(2H),4′-pyrano[2,3-d]pyrazole]-5′-carbonitrile

In the crystal structure of the title compound, C21H15N5O2, the planar indolone unit and the pyran ring are almost perpendicular to each other [dihedral angle = 89.41 (2)°], and the pyrazole and phenyl rings are oriented at an angle of 25.74 (1)°. The mol­ecular packing is stabilized by inter- and intra­molecular C—H⋯O, N—H⋯O and C—H⋯π hydrogen bonds

Pengembangan Durian Batu Busuak, Kelurahan Lambung Bukit, Kota Padang

Kampung Batu Busuk Kelurahan Lambung Bukit Kecamatan Pauh merupakan salah satu sentra produksi buah durian di Sumatera Barat.Walaupun nama durian Batu Busuk sudah terkenal dan banyak dicari oleh pencinta durian pada saat musim durian, namun belum ada upaya untuk mengangkat durian Batu Busuk untuk bisa dikenal lebih luas. Langkah awal untuk mengangkat nama durian Batu Busuk adalah membenahi identitas varietas durian Batu Busuk, karena sampai sekarang karakteristik dari durian Batu Busuk yang sesungguhnya tidak jelas.Tanaman yang sudah dikarakterisasi juga perlu diperbanyak agar kedepan, buah yang dihasilkan oleh kelurahan Batu Busuk adalah buah dengan karakter unggul tertentu. Masyarakat atau petani yang menanam durian juga perlu memiliki keahlian memperbanyak durian unggulnya. Penerapan ipteks yang dilakukan pada kegiatan pengabdian pada masyarakat ini menggunakan beberapa metode antara lain penyuluhan, demonstrasi aplikasi dan demplot pembibitan durian. Hasil kegiatan menunjukkan bahwa kegiatan penyuluhan yang dilakukan berhasil meningkatkan pengetahuan masyarakat Batu Busuk mengenai arti penting pendaftaran varietas durian. Kegiatan demplot pembibitan durian dan demonstrasi teknik sambung meningkatkan pengetahuan dan keterampilan masyarakat mengenai propagasi durian

Glucose Amplifies Fatty Acid-Induced Endoplasmic Reticulum Stress in Pancreatic β-Cells via Activation of mTORC1

BACKGROUND: Palmitate is a potent inducer of endoplasmic reticulum (ER) stress in beta-cells. In type 2 diabetes, glucose amplifies fatty-acid toxicity for pancreatic beta-cells, leading to beta-cell dysfunction and death. Why glucose exacerbates beta-cell lipotoxicity is largely unknown. Glucose stimulates mTORC1, an important nutrient sensor involved in the regulation of cellular stress. Our study tested the hypothesis that glucose augments lipotoxicity by stimulating mTORC1 leading to increased beta-cell ER stress. PRINCIPAL FINDINGS: We found that glucose amplifies palmitate-induced ER stress by increasing IRE1alpha protein levels and activating the JNK pathway, leading to increased beta-cell apoptosis. Moreover, glucose increased mTORC1 activity and its inhibition by rapamycin decreased beta-cell apoptosis under conditions of glucolipotoxicity. Inhibition of mTORC1 by rapamycin did not affect proinsulin and total protein synthesis in beta-cells incubated at high glucose with palmitate. However, it decreased IRE1alpha expression and signaling and inhibited JNK pathway activation. In TSC2-deficient mouse embryonic fibroblasts, in which mTORC1 is constitutively active, mTORC1 regulated the stimulation of JNK by ER stressors, but not in response to anisomycin, which activates JNK independent of ER stress. Finally, we found that JNK inhibition decreased beta-cell apoptosis under conditions of glucolipotoxicity. CONCLUSIONS/SIGNIFICANCE: Collectively, our findings suggest that mTORC1 mediates glucose amplification of lipotoxicity, acting through activation of ER stress and JNK. Thus, mTORC1 is an important transducer of ER stress in beta-cell glucolipotoxicity. Moreover, in stressed beta-cells mTORC1 inhibition decreases IRE1alpha protein expression and JNK activity without affecting ER protein load, suggesting that mTORC1 regulates the beta-cell stress response to glucose and fatty acids by modulating the synthesis and activity of specific proteins involved in the execution of the ER stress response. This novel paradigm may have important implications for understanding beta-cell failure in type 2 diabetes