Evaluating GPT-4’s Proficiency in Addressing Cryptography Examinations

In the rapidly advancing domain of artificial intelligence, ChatGPT, powered by the GPT-4 model, has emerged as a state-of-the-art interactive agent, exhibiting substantial capabilities across various domains. This paper aims to assess the efficacy of GPT-4 in addressing and solving problems found within cryptographic examinations. We devised a multi-faceted methodology, presenting the model with a series of cryptographic questions of varying complexities derived from real academic examinations. Our evaluation encompasses both classical and modern cryptographic challenges, focusing on the model\u27s ability to understand, interpret, and generate correct solutions while discerning its limitations. The model was challenged with a spectrum of cryptographic tasks, earning 201 out of 208 points by solving fundamental queries inspired by an oral exam, 80.5 out of 90 points on a written Crypto 1 exam, and 287 out of 385 points on advanced exercises from the Crypto 2 course. The results demonstrate that while GPT-4 shows significant promise in grasping fundamental cryptographic concepts and techniques, certain intricate problems necessitate domain-specific knowledge that may sometimes lie beyond the model\u27s general training. Insights from this study can provide educators, researchers, and examiners with a deeper understanding of how cutting-edge AI models can be both an asset and a potential concern in academic settings related to cryptology. To enhance the clarity and coherence of our work, we utilized ChatGPT-4 to help us in formulating sentences in this paper

Influence of the post-harvest storage time on the multi-biological potential, phenolic and pyrrolizidine alkaloid content of comfrey (Symphytum officinale L.) roots collected from different European regions

Comfrey (Symphytum officinale L.) roots are well-known bioactive ingredients included in various cosmeceutical and pharmaceutical preparations. In this study, the influence of the post-harvest storage on the chemico-biological potential of roots collected from different European regions and stored for up to six months was investigated. Total phenolic content (TPC) and total phenolic acid content (TPAC) were spectrophotometrically estimated, whereas the levels of individual phenolic and pyrrolizidine alkaloidal markers were determined by HPLC-DAD and HPLC-MS/MS, respectively. The changes in the biological potential was tracked via antioxidant (DPPH, ABTS, CUPRAC, and FRAP) and anti-enzymatic (cholinesterase, tyrosinase, glucosidase, and amylase) assays. TPC and TPAC varied from 6.48-16.57 mg GAE/g d.w. root and from 2.67-9.03 mg CAE/g, respectively. The concentration of the four phenolics (rosmarinic acid, globoidnan A, globoidnan B, rabdosiin) and six pyrrolizidine alkaloids generally showed maximum values at 1-3 months, after which their levels significantly decreased. With respect to the bioassays, the samples showed a wide range of antioxidant and anti-enzymatic effects; however, a direct storage time-bioactivity relationship was not observed. Similar conclusions were also revealed by the multivariate and correlation analyses. Our study could improve the current knowledge of the shelf-life properties of comfrey-based products and enhance their industrial exploitation

Tango Controls and data pipeline for petawatt laser experiments

The Centre for Advanced Laser Applications in Garching, Germany, is home to the ATLAS-3000 multi-petawatt laser, dedicated to research on laser particle acceleration and its applications. A control system based on Tango Controls is implemented for both the laser and four experimental areas. The device server approach features high modularity, which, in addition to the hardware control, enables a quick extension of the system and allows for automated data acquisition of the laser parameters and experimental data for each laser shot. In this paper we present an overview of our implementation of the control system, as well as our advances in terms of experimental operation, online supervision and data processing. We also give an outlook on advanced experimental supervision and online data evaluation – where the data can be processed in a pipeline – which is being developed on the basis of this infrastructure

Antioxidant and Anticancer Activity of <i>Vitis vinifera</i> Extracts in Breast Cell Lines

Vitis vinifera extracts have been shown to possess antioxidant activity because of their polyphenol content. In addition, their therapeutic potential against several diseases, including cancer, has been reported. In this study, we produced twelve extracts from the seeds, fruit, leaves, and wood of the Vitis vinifera Airen variety using different extraction methodologies and measured their total polyphenol content (TPC). We also determined their antioxidant and antiproliferative effects against normal cells and evaluated the most potent extract against a panel of breast cancer cell lines. We found that the extracts produced by the seeds of Vitis vinifera had a higher TPC compared to the other parts of the plant. Most extracts produced from seeds had antioxidant activity and did not show cytotoxicity against normal breast cells. The extract produced from whole organic seeds of white grape showed the best correlation between the dose and the ROS inhibition at all time points compared to the other seed extracts and also had antiproliferative properties in estrogen-receptor-positive MCF-7 breast cancer cells. Its mechanism of action involves inhibition of proteins Bcl-2, Bcl-xL, and survivin, and induction of apoptosis. Further investigation of the constituents and activity of Vitis vinifera extracts may reveal potential pharmacological applications of this plant

Is comfrey root more than toxic pyrrolizidine alkaloids? Salvianolic acids among antioxidant polyphenols in comfrey (Symphytum officinale L.) roots

Comfrey root preparations are used for the external treatment of joint distortions and myalgia, due to its analgesic and anti-inflammatory properties. Up to date, key activity-determining constituents of comfrey root extracts have not been completely elucidated. Therefore, we applied different approaches to further characterize a comfrey root extract (65% ethanol). The phenolic profile of comfrey root sample was characterized by HPLC-DAD-QTOF-MS/MS. Rosmarinic acid was identified as main phenolic constituent (7.55 mg/g extract). Moreover, trimers and tetramers of caffeic acid (isomers of salvianolic acid A, B and C) were identified and quantified for the first time in comfrey root. In addition, pyrrolizidine alkaloids were evaluated by HPLC-QQQ-MS/MS and acetylintermedine, acetyllycopsamine and their N-oxides were determined as major pyrrolizidine alkaloids in the comfrey root sample. Lastly, the antioxidant activity was determined using four assays: DPPH and ABTS radicals scavenging assays, reducing power assay and 15-lipoxygenase inhibition assay. Comfrey root extract exhibited significant antioxidant activities when compared to known antioxidants. Thus, comfrey root is an important source of phenolic compounds endowed with antioxidant activity which may contribute to the overall bioactivity of Symphytum preparations

Decryption of historical manuscripts : the DECRYPT project

Many historians and linguists are working individually and in an uncoordinated fashion on the identification and decryption of historical ciphers. This is a time-consuming process as they often work without access to automatic methods and processes that can accelerate the decipherment. At the same time, computer scientists and cryptologists are developing algorithms to decrypt various cipher types without having access to a large number of original ciphertexts. In this paper, we describe the DECRYPT project aiming at the creation of resources and tools for historical cryptology by bringing the expertise of various disciplines together for collecting data, exchanging methods for faster progress to transcribe, decrypt and contextualize historical encrypted manuscripts. We present our goals and work-in progress of a general approach for analyzing historical encrypted manuscripts using standardized methods and a new set of state-of-the-art tools. We release the data and tools as open-source hoping that all mentioned disciplines would benefit and contribute to the research infrastructure of historical cryptology

Symphytum officinale L. : liquid-liquid chromatography isolation of caffeic acid oligomers and evaluation of their influence on pro-inflammatory cytokine release in LPS-stimulated neutrophils

Ethnopharmacological relevance: Symphytum officinale L. (comfrey, Boraginaceae) has been traditionally used for millennia in joint distortions, myalgia, bone fractures and hematomas. However, key activity-determining constituents and molecular mechanisms underlying its use have not been completely elucidated. Aim of the study: The objective of this study was to isolate and identify the major compounds from a hydro-ethanolic root extract of S. officinale and evaluate their antioxidant potential, alongside their effect on the cytokine production of ex vivo stimulated neutrophils, thus providing scientific support for the traditional use of comfrey root. Material and methods: Four caffeic acid oligomers were isolated from comfrey roots by liquid-liquid chroma-tography, their structures being established by MS and NMR analyses. In vitro antioxidant evaluation was per-formed by DPPH and ABTS assays. The cytotoxicity of isolated compounds was established by flow cytometry. The effect on cytokine release, such as interleukin (IL)-1β, IL-8 and tumor necrosis factor alpha (TNF-α), in lipopolysaccharide (LPS)-stimulated neutrophils was determined by enzyme-linked immunosorbent assay (ELISA). Results: The main constituents found in comfrey root were represented by four caffeic acid oligomers, namely globoidnan B (1), rabdosiin (2), rosmarinic acid (3) and globoidnan A (4). Rabdosiin, globoidnans A and B were isolated for the first time from S. officinale. In the in vitro antioxidant tests, compound 2 was the most active, with EC50 values in DPPH and ABTS assays of 29.14 ±0.43 and 11.13 ±0.39, respectively. Neutrophils’ viability over the tested concentration domain of 12.5–50 μM was not altered. At 50 μM, all compounds significantly inhibited IL-1β release, with compound 3 (45.60% release vs. LPS stimulated neutrophils) being the most active, followed by compounds 1 (53.85%), 2 (69.89%) and 4 (60.68%). Conclusions: The four caffeic acid oligomers reported in S. officinale root may contribute to the overall anti- inflammatory activity for which comfrey preparations are used in traditional medicine