Clinical significance of NCOA5 gene rs2903908 polymorphism in Behçet`s disease

Behçet’s disease (BD) is an autoimmune multisystemic disease. The precise etiology of BD is not fully understood; however, it is thought that interactions between genetic and environmental factors play an essential role in its pathogenesis. The nuclear receptor coactivator-5 (NCOA5) gene encodes a coregulator for nuclear receptor sub- family 1 group D member 2 (NR1D2) and estrogen receptor 1 and 2 (ESR1 and ESR2). Also, the NCOA5 gene insufficiency leads to an elevated expression of IL-6, and increased levels of IL-6 were found to be related to the pathogenesis of BD. In this study, we aimed to clarify the impact of the NCOA5 rs2903908 polymorphism on susceptibility and clinical findings of BD. This study included 671 participants (300 BD patients and 371 healthy controls). The analyses of NCOA5 rs2903908 polymorphism was performed by using the TaqMan allelic discrim- ination assay. The frequency of TT genotype of the NCOA5 rs2903908 polymorphism was found significantly higher in BD patients compared to those in healthy controls (p=0.016, OR=1.46, 95 % CI=1.08–1.99). Also, the frequencies of CT genotype was observed significantly higher in BD patients with genital ulceration and uveitis than without genital ulceration and uveitis (p=0.002 and p=0.005, respectively). The most significant association was found between C allele frequencies of BD patients with and without uveitis (p=0.0001). Our study represents e first time that the NCOA5 rs2903908 polymorphism seemed to be linked to BD susceptibility and clinical findings

Effect of alternative modes of application on microleakage of one-step self-etch adhesives

AbstractBackground/purposeContemporary one-step self-etch adhesives are certainly the most user-friendly adhesives; however, typically could not compete with the more traditional multi-step adhesives. This in vitro study evaluated the efficacy of two modes of application (double application or placement of a hydrophobic resin coating) for the reduction of microleakage, using three different one-step self-etch adhesives (AdheSE One VivaPen, Ivoclar Vivadent, Schaan, Liechtenstein; Xeno V, Dentsply De Trey, Konstanz, Germany; OptiBond All-In-One, Kerr Italia S.r.l, Scafati, Italy).Materials and methodsWe prepared Class V cavities with the occlusal margin in enamel and gingival margin in dentin on both buccal and lingual surfaces of 108 human molar teeth. Cavities were divided into nine groups according to the combination of adhesives and application modes used (n = 24). For applying adhesives, we followed three types of procedures: (1) according to the manufacturers’ directions; (2) using a double-application method; and (3) with the additional placement of a hydrophobic resin layer after following the manufacturers’ directions. We restored the cavities with a composite resin. After thermocycling (500 cycles at 5°C/55°C) and immersion in 0.5% basic fuchsin, we sectioned the teeth and evaluated them for microleakage using a stereo microscope (20×). Enamel and dentin margins were scored separately, and we analyzed the data using Kruskal–Wallis, Bonferroni-corrected Mann–Whitney U, and Wilcoxon signed-rank tests.ResultsApplication of a hydrophobic resin over the cured Xeno V adhesive decreased the amount of microleakage at the enamel margins, compared with the application according to the manufacturer’s instructions (P = 0.005). Both application modes decreased the amount of microleakage at the dentin margins with AdheSE One VivaPen adhesive, compared with application according to the manufacturer’s instructions (P < 0.001). With the OptiBond All-In-One adhesive, no significant difference between application modes was observed.ConclusionAlternative modes of application did not adversely affect the sealing ability of one-step self-etch adhesives. Depending on the type of adhesive, alternative modes of application may contribute to low microleakage values

A new method for automatic counting of ovarian follicles on whole slide histological images based on convolutional neural network

The ovary is a complex endocrine organ that shows significant structural and functional changes in the female reproductive system over recurrent cycles. There are different types of follicles in the ovarian tissue. The reproductive potential of each individual depends on the numbers of these follicles. However, genetic mutations, toxins, and some specific drugs have an effect on follicles. To determine these effects, it is of great importance to count the follicles. The number of follicles in the ovary is usually counted manually by experts, which is a tedious, time-consuming and intense process. In some cases, the experts count the follicles in a subjective way due to their knowledge. In this study, for the first time, a method has been proposed for automatically counting the follicles of ovarian tissue. Our method primarily involves filter-based segmentation applied to whole slide histological images, based on a convolutional neural network (CNN). A new method is also proposed to eliminate the noise that occurs after the segmentation process and to determine the boundaries of the follicles. Finally, the follicles whose boundaries are determined are classified. To evaluate its performance, the results of the proposed method were compared with those obtained by two different experts and the results of the Faster R-CNN model. The number of follicles obtained by the proposed method was very close to the number of follicles counted by the experts. It was also found that the proposed method was much more successful than the Faster R-CNN model

Evaluation of Suspected Macrolide Allergies in Children

Objective: Macrolides are often accepted as safe antibiotics due to their low allergenicity. However, studies on macrolides, particularly studies evaluating cross-reactivity in macrolides, are highly rare in children. This study aimed to evaluate the clinical manifestations, confirmation rate, and frequency of cross-reactivity in children admitted with suspicious clarithromycin or azithromycin allergy

Human and bovine pulp-derived cell reactions to dental resin cements

Joint Meeting of the Continental-European-Division, Scandinavian-Division and Israeli-Division of the International-Association-for-Dental-Research (IADR) -- SEP 10-12, 2009 -- Munich, GERMANYSENGUN, Abdulkadir/0000-0002-6053-160XWOS: 000311362300009PubMed: 22290062Objectives The objective of this study was to evaluate the cytotoxic reaction of transfected human pulp derived cells (tHPDC) and transfected bovine pulp derived cells (tBPDC) after exposure to resin cements [RelyX UnicemClicker (RX), MaxCem (MC), Panavia F 2.0 (PF), BisCem (BC), and Bistite II DC (BII)] and to compare it to the generation of reactive oxygen species (ROS). Materials and methods Set materials were extracted in culture medium, cell survival as a measure of cytotoxicity was determined photometrically using crystal violet after cells were exposed to the extracts for 24 h. The generation of ROS was detected by flow cytometry after cells were exposed to extract dilutions for 1 h. Results The ranking of the least to the most cytotoxic material was: RX < BII < PF < BC < MC for both cell lines, but for tHPDC, only MC and PF eluates were different from untreated controls. Generally, tBPDC were more susceptible to materials than tHPDC, but only for RX and BC was this difference statistically significant. All undiluted extracts increased ROS production in both cell lines but to a higher amount in tHPDC than in tBPDC. Conclusions tHPDC reacted less sensitive than tBPDC in the cytotoxicity test but with the same rank order of materials. In contrast, the cellular oxidative stress reaction was more pronounced in tHPDC than in tBPDC. Clinical relevance Depending on the residual dentine layer in deep cavities, biologically active resin monomers or additives released from resin cements may influence the dentine-pulp complex, for instance, its regenerative and reparative capacities.Int Assoc Dent Res (IADR), Continental European Div, Int Assoc Dent Res (IADR), Scandinavian Div, Int Assoc Dent Res (IADR), Israeli Di