The Effect of Chronic Sleep Deprivation on Tumor Necrosis Factor Alpha and Bone Health in Peri-Menopausal Rats

Post-menopausal osteoporosis is a common syndrome in the United States. The cessation of estrogen signaling coupled with the production of pro-inflammatory cytokines from sleep deprivation leads to an even greater risk of post-menopausal osteoporosis by creating an imbalance between osteoblasts and osteoclasts. With estrogen no longer present to regulate the concentration of osteoclasts and pro-inflammatory cytokines increasing production of osteoclasts, bone is degraded at a faster rate than it is formed. One of the most common treatments for osteoporosis is Zolendronate (a nitrogenous bisphosphonate), which decreases the number of osteoclasts in bone. This preliminary study looked at the effects on the concentration of tumor necrosis factor alpha-type (TNFα), a pro-inflammatory cytokine, and bone strength due to Zolendronate and sleep deprivation in thirty-two ovariectomized Wistar rats. After a five-week sleep deprivation protocol, TNFα concentrations were determined by enzyme-linked immunoassay and bone strengths were determined by a three point bending test. There were no significant differences in bone strength, and the only significant difference in serum concentrations of TNFα (P\u3c.01) was with the group that received Zolendronate. While we expected that the sleep deprived and sleep deprived with Zolendronate groups would have significantly higher TNFα concentrations we purpose an over-exhausted immune system is responsible our low concentrations. The reason the Zolendronate group had a significantly higher TNFα level could have been due to a transient fever caused by the drug. Further research measuring the changes in cytokine concentration throughout a longer sleep deprivation protocol needs to be done

Patterns of Genetic Variation in Southern Appalachian Populations of Athyrium filix‐femina var. asplenioides (Dryopteridaceae)

Allozyme variation (17 loci coding 11 enzymes) was investigated in 14 populations of the fern Athyrium filix‐femina var. asplenioides arrayed at differing elevations and latitudes in the southern Appalachians. Allozyme fingerprints showed that asplenioides individuals comprise meandering, overlapping clones usually ≤3 m in extent, occasionally forming larger clones of up to 17 m. Levels of genetic variability in populations (means: , , ) were near the averages for both ferns and seed plants. General conformance to Hardy‐Weinberg expectations indicated a predominantly outcrossing mating system. Hierarchical F statistic analysis and occasional deficits and excesses of heterozygotes indicated population substructure. Similar allele frequencies across all populations resulted in low to moderate values (mean ; ) and high values of genetic similarity (mean ; mean ). Hierarchical analysis indicated that neither regional proximity ( ) nor elevation ( ) contributed substantially to divergence among populations ( ), a result corroborated by UPGMA analysis that clustered together populations from different regions and of different elevational class. Southern Appalachian asplenioides differed from more eastern asplenioides populations of the piedmont and coastal plain in having higher frequencies of Pgm‐2c and Tpi‐2B, alleles characteristic of the more northern variety angustum. Nonetheless, genetic distinctness of the two varieties was maintained. We hypothesize that higher frequencies of angustum alleles in the southern Appalachian asplenioides populations are the result of introgression from angustum that persisted at high elevations as both taxa migrated northward following the retreat of the Wisconsinan glacier

The LCA Commons—How an Open-Source Repository for US Federal Life Cycle Assessment (LCA) Data Products Advances Inter-Agency Coordination

Life cycle assessment (LCA) is a flexible and powerful tool for quantifying the total environmental impact of a product or service from cradle-to-grave. The US federal government has developed deep expertise in environmental LCA for a range of applications including policy, regulation, and emerging technologies. LCA professionals from across the government have been coordinating the distributed LCA expertise through a community of practice known as the Federal LCA Commons. The Federal LCA Commons has developed open data infrastructure and workflows to share knowledge and align LCA methods. This data infrastructure is a key component to creating a harmonized network of LCA capacity from across the federal government

The LCA Commons—How an Open-Source Repository for US Federal Life Cycle Assessment (LCA) Data Products Advances Inter-Agency Coordination

Life cycle assessment (LCA) is a flexible and powerful tool for quantifying the total environmental impact of a product or service from cradle-to-grave. The US federal government has developed deep expertise in environmental LCA for a range of applications including policy, regulation, and emerging technologies. LCA professionals from across the government have been coordinating the distributed LCA expertise through a community of practice known as the Federal LCA Commons. The Federal LCA Commons has developed open data infrastructure and workflows to share knowledge and align LCA methods. This data infrastructure is a key component to creating a harmonized network of LCA capacity from across the federal government

Song matching, overlapping, and switching in the banded wren: the sender's perspective

Interpreting receiver responses to on-territory playback of aggressive signals is problematic. One solution is to combine such receiver-perspective experiments with a sender-perspective experiment that allows subjects to demonstrate how their choice of singing strategies is associated with their approach behavior. Here we report the results of a sender-perspective study on the banded wren (Thryothorus pleurostictus) and combine information on context and results of previous receiver-perspective experiments to clarify function. Territorial males were presented with a 5-min playback consisting of song types present in their repertoire. We assessed the degree to which the subjects' song-matching rate, overlapping rate, and song-type versatility were correlated with their approach latency, closeness of approach, latency to first retreat, and time spent close to the speaker. Male age, breeding stage, and features of the playback stimuli were also considered. Song matching was associated with rapid and close approach, consistent with the receiver-perspective interpretation of type matching as a conventional signal of aggressive motivation. Overlapping was associated with earlier retreat and together with the aversive receiver response to our previous overlapping playback experiment suggests that overlapping is a defensive withdrawal signal. High versatility was associated with slower first retreat from the speaker and high levels of reciprocal matching between subject and playback. Males with fledglings sang with particularly low versatility and approached the speaker aggressively, whereas males with nestlings overlapped more and retreated quickly. Finally, older males matched more but overlapped less. Copyright 2007, Oxford University Press.

Granulocyte Transfusions in Patients with Chronic Granulomatous Disease Undergoing Hematopoietic Cell Transplantation or Gene Therapy.

Granulocyte transfusions are sometimes used as adjunctive therapy for the treatment of infection in patients with chronic granulomatous disease (CGD). However, granulocyte transfusions can be associated with a high rate of alloimmunization, and their role in CGD patients undergoing hematopoietic cell transplantation (HCT) or gene therapy (GT) is unknown. We identified 27 patients with CGD who received granulocyte transfusions pre- (within 6 months) and/or post-HCT or GT in a retrospective survey. Twelve patients received granulocyte transfusions as a bridge to cellular therapy. Six (50%) of these patients had a complete or partial response. However, six of 10 (60%) patients for whom testing was performed developed anti-HLA antibodies, and three of the patients also had severe immune-mediated cytopenia within the first 100 days post-HCT or GT. Fifteen patients received granulocyte transfusions post-HCT only. HLA antibodies were not checked for any of these 15 patients, but there were no cases of early immune-mediated cytopenia. Out of 25 patients who underwent HCT, there were 5 (20%) cases of primary graft failure. Three of the patients with primary graft failure had received granulocyte transfusions pre-HCT and were subsequently found to have anti-HLA antibodies. In this small cohort of patients with CGD, granulocyte transfusions pre-HCT or GT were associated with high rates of alloimmunization, primary graft failure, and early severe immune-mediated cytopenia post-HCT or GT. Granulocyte transfusions post-HCT do not appear to confer an increased risk of graft failure