Toward a Multi-Dimensional Model of Adolescent Romantic Parasocial Attachment

Relationships with media figures—referred to as parasocial relationships—provide a means for adolescents to explore and define their romantic and sexual identities (Boon & Lomore, 2001; Engle & Kasser, 2005; Karniol, 2001). However, more often than not, adolescents’ romantic attachments to media figures are ignored or dismissed as frivolous in the scholarly literature on youth development (Caughey, 1984; Jenkins, 1992; Willis, 1972). In this paper, we introduce a theoretical model of adolescent romantic parasocial attachment (ARPA) designed to facilitate a comprehensive, developmentally-based line of research that improves our understanding of the ways adolescents experience parasocial romance and the influence their experiences may have on their lives. Implications for the study of adolescent romantic development and future research directions are proposed

Shifting Attitudes Towards Seeking Help in a Youth Development Program: A Program Evaluation

Abstract Youth development programs often suffer from a dearth of evaluation of the programs' long-term effects. To integrate more comprehensive and impactful evaluation in youth development programs, the present project evaluated how program participation affected caregivers' intentions to seek help for mental health issues in the future. Service utilization, perception of support, and satisfaction with the program were all included as predictors of intentions for seeking help. Using Structural Equation Modeling (SEM), an original model based on the theory of planned behavior was tested. It was found that program participation significantly increased families' willingness to seek help for child mental health issues. When testing the hypothesized mechanisms of this change, mediational models showed that support and satisfaction variables did not explain the increase of help-seeking intentions, contrary to predictions. Implications of these results for youth development programs and for professionals working with children and families in need of services are discussed

Distribution and Abundance of Introduced Fishes in Florida\u27s Charlotte Harbor Estuary

A growing number of non-native fishes have been introduced into Florida waters in recent years, yet little information has been available on their distribution and abundance in southwest Florida. The ichthyofauna of the Charlotte Harbor estuary, Florida, was intensively sampled from 1989 through 2007. We collected eight introduced fish taxa: African jewelfish (Hemichromis letourneuxi), blue tilapia (Oreochromis aureus), brown hoplo (Hoplosternum littorale), grass carp (Ctenopharyngodon idella), Mayan cichlid (Cichlasoma urophthalmus), sailfin catfishes (Pterygoplichthys spp.), spotted tilapia (Tilapia mariae), and walking catfish (Clarias batrachus). These fishes were found principally in tidal rivers, especially the Caloosahatchee River. Other introduced species, such as Asian swamp eel (Monopterus albus), blackchin tilapia (Sarotherodon melanotheron), and pike killifish (Belonesox belizanus), are known to occur in neighboring bay systems but have yet to be reported in the Charlotte Harbor estuary. Continued monitoring will help us detect additional species that are introduced to the estuary, expansions in the ranges of documented species, and assemblage-level changes

Cross-Sectional Survey of Perceived Barriers Among Community Pharmacists Who Do Not Immunize, in Wayne County, Michigan

Introduction: The goal of the study was to identify perceived barriers to implementation of vaccination services encountered by independent and small-chain community pharmacies in an urban setting. Methods: Pharmacists in independent and small-chain pharmacies located in 29 Michigan ZIP codes were visited and asked to complete a 5- to 10-min semi-structured interview. Results: A total of 93 independent and 12 small-chain pharmacies participated (n = 105; 61%). The pharmacies filled an average of 700 prescriptions each week with 1.1 pharmacist full-time equivalents and 57 h of technician time. The most common services that participating pharmacies provided were dispensing outpatient medication (99%), medication therapy management (MTM, 65.7%), disease management or coaching (54.3%), point-of-care testing (34.3%), and dispensing medications to inpatient facilities (16.2%). Only seven pharmacies (6.7%) administered vaccinations. When pharmacists were asked to identify what it would take to start to administer vaccines, the most common responses were increased demand from patients (37.1%), adequate time (19%), appropriate space (17.1%), appropriate amount of staff (14.3%), change in attitudes or beliefs of the owner or pharmacists at that pharmacy (13.3%), increased profit related to vaccines (11.4%), and increased awareness among patients about the importance of vaccines (11.4%). The majority of pharmacies (65.3%) reported that only one factor would need to change to start to administer vaccines. Conclusion: Independent and small-chain community pharmacies in an urban, primarily low-income area identified several barriers that have prevented implementation of vaccination services. However, the majority of pharmacies reported that only one factor would need to change in order to begin to administer vaccines. Interventional efforts necessary to address commonly cited barriers may include providing education to pharmacists about the need for community pharmacy-based immunization programs in addition to services provided by physician offices, as well as the importance of proactively providing immunization-related recommendations to patients

The Effects of Dried Distillers Grains on Heifers Consuming Low or High Quality Forage

Two forage sources, high and low quality, were used to evaluate effects of five levels of dried distillers grains on forage intake. Ninety heifer calves were fed high or low quality forage, supplemented with 0, 1.5, 3, 4.5, or 6 lb DM dried distillers grains. Forage intakes linearly decreased as dried distillers grains increased. Average daily gain increased linearly with increased dried distillers grains indicating that dried distillers grains can be a protein and energy supplement source and a substitute for forage. Dried distillers grains are an economical supplement to cattle on either high or low quality forage diets

The Effects of Dried Distillers Grains on Heifers Consuming Low or High Quality Forage

Two forage sources, high and low quality, were used to evaluate effects of five levels of dried distillers grains on forage intake. Ninety heifer calves were fed high or low quality forage, supplemented with 0, 1.5, 3, 4.5, or 6 lb DM dried distillers grains. Forage intakes linearly decreased as dried distillers grains increased. Average daily gain increased linearly with increased dried distillers grains indicating that dried distillers grains can be a protein and energy supplement source and a substitute for forage. Dried distillers grains are an economical supplement to cattle on either high or low quality forage diets

Associations Between Adolescent Chronic Pain and Prescription Opioid Misuse in Adulthood

Prescription opioid misuse is a serious public health concern, yet antecedent factors are poorly described. Using data from the National Longitudinal Study of Adolescent to Adult Health (N = 14,784), we examined the longitudinal relationship between a history of adolescent chronic pain and the odds of misusing prescription opioids in adulthood. The primary predictor variable was chronic pain status during adolescence. The primary outcome variables were prescription opioid misuse during early adulthood and adulthood. Multivariate models controlled for known risk factors of opioid misuse, including sociodemographics (sex, race, and ethnicity), adolescent mental health symptoms (anxiety, depression), adolescent self-reported physical health status, adolescent substance use/abuse, childhood trauma, and adult legitimate opioid use. We found that adults with a history of adolescent chronic pain were more likely to misuse opioids than those without history of chronic pain, even after controlling for other known risk factors. Further, we found that among individuals with history of adolescent chronic pain that race (white), other substance use, and exposure to trauma were risk factors for later opioid misuse. Longitudinal associations between adolescent chronic pain and subsequent adult prescription opioid misuse highlight the need for early targeted screening and prevention efforts that may reduce later opioid misuse. Perspective: Using a large, nationally representative sample, we found that chronic pain during adolescence was an independent risk factor for opioid misuse in adulthood, over and above other known risk factors. Furthermore, among those individuals with adolescent chronic pain, substance use, exposure to trauma, and race were associated with opioid misuse.</p

Correlation functions quantify super-resolution images and estimate apparent clustering due to over-counting

We present an analytical method to quantify clustering in super-resolution localization images of static surfaces in two dimensions. The method also describes how over-counting of labeled molecules contributes to apparent self-clustering and how the effective lateral resolution of an image can be determined. This treatment applies to clustering of proteins and lipids in membranes, where there is significant interest in using super-resolution localization techniques to probe membrane heterogeneity. When images are quantified using pair correlation functions, the magnitude of apparent clustering due to over-counting will vary inversely with the surface density of labeled molecules and does not depend on the number of times an average molecule is counted. Over-counting does not yield apparent co-clustering in double label experiments when pair cross-correlation functions are measured. We apply our analytical method to quantify the distribution of the IgE receptor (Fc{\epsilon}RI) on the plasma membranes of chemically fixed RBL-2H3 mast cells from images acquired using stochastic optical reconstruction microscopy (STORM) and scanning electron microscopy (SEM). We find that apparent clustering of labeled IgE bound to Fc{\epsilon}RI detected with both methods arises from over-counting of individual complexes. Thus our results indicate that these receptors are randomly distributed within the resolution and sensitivity limits of these experiments.Comment: 22 pages, 5 figure

Cyclic Rhamnosylated Elongation Factor P Establishes Antibiotic Resistance in \u3cem\u3ePseudomonas aeruginosa\u3c/em\u3e

Elongation factor P (EF-P) is a ubiquitous bacterial protein that is required for the synthesis of poly-proline motifs during translation. In Escherichia coli and Salmonella enterica, the posttranslational β-lysylation of Lys34 by the PoxA protein is critical for EF-P activity. PoxA is absent from many bacterial species such as Pseudomonas aeruginosa, prompting a search for alternative EF-P posttranslation modification pathways. Structural analyses of P. aeruginosa EF-P revealed the attachment of a single cyclic rhamnose moiety to an Arg residue at a position equivalent to that at which β-Lys is attached to E. coli EF-P. Analysis of the genomes of organisms that both lack poxA and encode an Arg32-containing EF-P revealed a highly conserved glycosyltransferase (EarP) encoded at a position adjacent to efp. EF-P proteins isolated from P. aeruginosa ΔearP, or from a ΔrmlC::acc1 strain deficient in dTDP-l-rhamnose biosynthesis, were unmodified. In vitro assays confirmed the ability of EarP to use dTDP-l-rhamnose as a substrate for the posttranslational glycosylation of EF-P. The role of rhamnosylated EF-P in translational control was investigated in P. aeruginosa using a Pro4-green fluorescent protein (Pro4GFP) in vivo reporter assay, and the fluorescence was significantly reduced in Δefp, ΔearP, and ΔrmlC::acc1 strains. ΔrmlC::acc1, ΔearP, and Δefp strains also displayed significant increases in their sensitivities to a range of antibiotics, including ertapenem, polymyxin B, cefotaxim, and piperacillin. Taken together, our findings indicate that posttranslational rhamnosylation of EF-P plays a key role in P. aeruginosa gene expression and survival