Curation Principles Derived from the Analysis of the SBOL iGEM Data Set

As an engineering endeavor, synthetic biology requires effective sharing of genetic design information that can be reused in the construction of new designs. While there are a number of large community repositories of design information, curation of this information has been limited. This in turn limits the ways in which design information can be put to use. The aim of this work was to improve this situation by creating a curated library of parts from the International Genetically Engineered Machines (iGEM) registry data set. To this end, an analysis of the Synthetic Biology Open Language (SBOL) version of the iGEM registry was carried out using four different approaches&mdash;simple statistics, SnapGene autoannotation, SYNBICT autoannotation, and expert analysis&mdash;the results of which are presented herein. Key challenges encountered include the use of free text, insufficient part provenance, part duplication, lack of part removal, and insufficient continuous curation. On the basis of these analyses, the focus has shifted from the creation of a curated iGEM part library to instead the extraction of a set of lessons, which are presented here. These lessons can be exploited to facilitate the creation and curation of other part libraries using a simpler and less labor intensive process. &nbsp;</p

Double Dutch: A Tool for Designing Combinatorial Libraries of Biological Systems

Recently, semirational approaches that rely on combinatorial assembly of characterized DNA components have been used to engineer biosynthetic pathways. In practice, however, it is not practical to assemble and test millions of pathway variants in order to elucidate how different DNA components affect the behavior of a pathway. To address this challenge, we apply a rigorous mathematical approach known as design of experiments (DOE) that can be used to construct empirical models of system behavior without testing all variants. To support this approach, we have developed a tool named Double Dutch, which uses a formal grammar and heuristic algorithms to automate the process of DOE library design. Compared to designing by hand, Double Dutch enables users to more efficiently and scalably design libraries of pathway variants that can be used in a DOE framework and uniquely provides a means to flexibly balance design considerations of statistical analysis, construction cost, and risk of homologous recombination, thereby demonstrating the utility of automating decision making when faced with complex design trade-offs

Decay properties of tau leptons measured at the Z0 resonance

Contains fulltext : 27577.PDF (publisher's version ) (Open Access

Search for lepton flavour violation in Z0 decays

Contains fulltext : 27575.PDF (publisher's version ) (Open Access

Determination of αs\alpha_{s} from jet multiplicities measured on the Z0^{0} resonance

Whatever the field, major change implies as much as deep transformation of thinking i.e to put in network new skills in particular, those made available by the availability of new technologies. In this context, only one individual cannot join together all necessary skills. It becomes clear that the goal of space valorization cannot be considered without relational investment between the partners and their accumulated background. The local empowerment concerns a collective phenomenon of acquisition and development of skills rests on an learning way appropriate to the potential of the territory to form or promote. The local mediation sets for objective the creation of relations between pre-existent skills and their transfer between the actors, individuals or groups, private or public. But the capacity of the territory to solve these problems will depend closely on the richness of the network and co-operations which it shelters or it will suggest