3,986 research outputs found
Structural Properties of the Caenorhabditis elegans Neuronal Network
Despite recent interest in reconstructing neuronal networks, complete wiring
diagrams on the level of individual synapses remain scarce and the insights
into function they can provide remain unclear. Even for Caenorhabditis elegans,
whose neuronal network is relatively small and stereotypical from animal to
animal, published wiring diagrams are neither accurate nor complete and
self-consistent. Using materials from White et al. and new electron micrographs
we assemble whole, self-consistent gap junction and chemical synapse networks
of hermaphrodite C. elegans. We propose a method to visualize the wiring
diagram, which reflects network signal flow. We calculate statistical and
topological properties of the network, such as degree distributions, synaptic
multiplicities, and small-world properties, that help in understanding network
signal propagation. We identify neurons that may play central roles in
information processing and network motifs that could serve as functional
modules of the network. We explore propagation of neuronal activity in response
to sensory or artificial stimulation using linear systems theory and find
several activity patterns that could serve as substrates of previously
described behaviors. Finally, we analyze the interaction between the gap
junction and the chemical synapse networks. Since several statistical
properties of the C. elegans network, such as multiplicity and motif
distributions are similar to those found in mammalian neocortex, they likely
point to general principles of neuronal networks. The wiring diagram reported
here can help in understanding the mechanistic basis of behavior by generating
predictions about future experiments involving genetic perturbations, laser
ablations, or monitoring propagation of neuronal activity in response to
stimulation
Time-Series Ensemble Photometry and the Search for Variable Stars in the Open Cluster M11
This work presents the first large-scale photometric variability survey of
the intermediate age (~200 Myr) open cluster M11. Thirteen nights of data over
two observing seasons were analyzed (using crowded field and ensemble
photometry techniques) to obtain high relative precision photometry. In this
study we focus on the detection of candidate member variable stars for
follow-up studies. A total of 39 variable stars were detected and can be
categorized as follows: 1 irregular (probably pulsating) variable, 6 delta
Scuti variables, 14 detached eclipsing binary systems, 17 W UMa variables, and
1 unidentified/candidate variable. While previous proper motion studies allow
for cluster membership determination for the brightest stars, we find that
membership determination is significantly hampered below V=15,R=15.5 by the
large population of field stars overlapping the cluster MS. Of the brightest
detected variables that have a high likelihood of cluster membership, we find
five systems where further work could help constrain theoretical stellar
models, including one potential W UMa member of this young cluster.Comment: 38 pages, 13 figures, accepted for December 2005 AJ, high-resolution
version available upon reques
A Search for Exozodiacal Clouds with Kepler
Planets embedded within dust disks may drive the formation of large scale
clumpy dust structures by trapping dust into resonant orbits. Detection and
subsequent modeling of the dust structures would help constrain the mass and
orbit of the planet and the disk architecture, give clues to the history of the
planetary system, and provide a statistical estimate of disk asymmetry for
future exoEarth-imaging missions. Here we present the first search for these
resonant structures in the inner regions of planetary systems by analyzing the
light curves of hot Jupiter planetary candidates identified by the Kepler
mission. We detect only one candidate disk structure associated with KOI 838.01
at the 3-sigma confidence level, but subsequent radial velocity measurements
reveal that KOI 838.01 is a grazing eclipsing binary and the candidate disk
structure is a false positive. Using our null result, we place an upper limit
on the frequency of dense exozodi structures created by hot Jupiters. We find
that at the 90% confidence level, less than 21% of Kepler hot Jupiters create
resonant dust clumps that lead and trail the planet by ~90 degrees with optical
depths >~5*10^-6, which corresponds to the resonant structure expected for a
lone hot Jupiter perturbing a dynamically cold dust disk 50 times as dense as
the zodiacal cloud.Comment: 22 pages, 6 figures, Accepted for publication in Ap
Coherent Cancellation of Photothermal Noise in GaAs/AlGaAs Bragg Mirrors
Thermal noise is a limiting factor in many high-precision optical
experiments. A search is underway for novel optical materials with reduced
thermal noise. One such pair of materials, gallium arsenide and
aluminum-alloyed gallium arsenide (collectively referred to as AlGaAs), shows
promise for its low Brownian noise when compared to conventional materials such
as silica and tantala. However, AlGaAs has the potential to produce a high
level of thermo-optic noise. We have fabricated a set of AlGaAs crystalline
coatings, transferred to fused silica substrates, whose layer structure has
been optimized to reduce thermo-optic noise by inducing coherent cancellation
of the thermoelastic and thermorefractive effects. By measuring the
photothermal transfer function of these mirrors, we find evidence that this
optimization has been successful.Comment: 10 pages, 7 figure
Prolyl-peptidyl isomerase, Pin1, phosphorylation is compromised in association with the expression of the HFE polymorphic allele, H63D
AbstractThere is substantial interest in HFE gene variants as putative risk factors in neurodegenerative diseases such as Alzheimer disease (AD). Previous studies in cell models have shown the H63D HFE variant to result in increased cellular iron, oxidative stress, glutamate dyshomeostasis, and an increase in tau phosphorylation; all processes thought to contribute to AD pathology. Pin1 is a prolyl-peptidyl cis/trans isomerase that can regulate the dephosphorylation of the amyloid and tau proteins. Hyperphosphorylation of these later proteins is implicated in the pathogenesis of AD and Pin1 levels are reportedly decreased in AD brains. Because of the relationship between Pin1 loss of function by oxidative stress and the increase in oxidative stress in cells with the H63D polymorphism it was logical to interrogate a relationship between Pin1 and HFE status. To test our hypothesis that H63D HFE would be associated with less Pin1 activity, we utilized stably transfected human neuroblastoma SH-SY5Y cell lines expressing the different HFE polymorphisms. Under resting conditions, total Pin1 levels were unchanged between the wild type and H63D HFE cells, yet there was a significant increase in phosphorylation of Pin1 at its serine 16 residue suggesting a loss of Pin1 activity in H63D variant cells. To evaluate whether cellular iron status could influence Pin1, we treated the WT HFE cells with exogenous iron and found that Pin1 phosphorylation increased with increasing levels of iron. Iron exposure to H63D variant cells did not impact Pin1 phosphorylation beyond that already seen suggesting a ceiling effect. Because HFE H63D cells have been shown to have more oxidative stress, the cells were treated with the antioxidant Trolox which resulted in a decrease in Pin1 phosphorylation in H63D cells with no change in WT HFE cells. In a mouse model carrying the mouse equivalent of the H63D allele, there was an increase in the phosphorylation status of Pin1 providing in vivo evidence for our findings in the cell culture model. Thus, we have shown another cellular mechanism that HFE polymorphisms influence; further supporting their role as neurodegenerative disease modifiers
Are superflares on solar analogues caused by extra-solar planets?
Stellar flares with times more energy than the largest solar
flare have been detected from 9 normal F and G main sequence stars (Schaefer,
King & Deliyannis 1999). These superflares have durations of hours to days and
are visible from at least x-ray to optical frequencies. The absence of
world-spanning aurorae in historical records and of anomalous extinctions in
the geological record indicate that our Sun likely does not suffer superflares.
In seeking to explain this new phenomenon, we are struck by its similarity to
large stellar flares on RS Canum Venaticorum binary systems, which are caused
by magnetic reconnection events associated with the tangling of magnetic fields
between the two stars. The superflare stars are certainly not of this class,
although we propose a similar flare mechanism. That is, superflares are caused
by magnetic reconnection between fields of the primary star and a close-in
Jovian planet. Thus, by only invoking known planetary properties and
reconnection scenarios, we can explain the energies, durations, and spectra of
superflares, as well as explain why our Sun does not have such events.Comment: 13 pages, Accepted for publication in Ap
A Review of Target Mass Corrections
With recent advances in the precision of inclusive lepton--nuclear scattering
experiments, it has become apparent that comparable improvements are needed in
the accuracy of the theoretical analysis tools. In particular, when extracting
parton distribution functions in the large-x region, it is crucial to correct
the data for effects associated with the nonzero mass of the target. We present
here a comprehensive review of these target mass corrections (TMC) to structure
functions data, summarizing the relevant formulas for TMCs in electromagnetic
and weak processes. We include a full analysis of both hadronic and partonic
masses, and trace how these effects appear in the operator product expansion
and the factorized parton model formalism, as well as their limitations when
applied to data in the x->1 limit. We evaluate the numerical effects of TMCs on
various structure functions, and compare fits to data with and without these
corrections.Comment: 41 pages, 13 figures; minor updates to match published versio
The stimulating effects of ethanol on ventral tegmental area dopamine neurons projecting to the ventral pallidum and medial prefrontal cortex in female Wistar rats: regional difference and involvement of serotonin-3 receptors
RATIONALE:
The ventral tegmental area (VTA) mediates the local stimulating effects of ethanol (EtOH) in a region-dependent manner, with EtOH administration in the posterior but not anterior VTA stimulating the mesolimbic system. The serotonin-3 (5-HT(3)) receptor has been involved in the effects of EtOH on the mesolimbic system.
OBJECTIVES:
The current study tested the hypothesis that EtOH would stimulate mesopallidal and mesocortical dopamine neurons in the posterior but not anterior VTA and that the stimulating effects of EtOH in the VTA would involve activation of local 5-HT(3) receptors.
METHODS:
Wistar female rats were surgically implanted with two cannulae, one in one sub-region of the VTA for microinjection and the other in the ventral pallidum (VP) or medial prefrontal cortex (mPFC) for microdialysis. Artificial cerebrospinal fluid or EtOH (200 mg%; 44 mM) was microinjected in the anterior or posterior VTA, and extracellular dopamine was measured in the VP or mPFC with microdialysis-HPLC.
RESULTS:
EtOH injections in the posterior but not anterior VTA significantly increased extracellular dopamine levels in the VP and mPFC. Co-injections of the 5-HT(3) receptor antagonist ICS-205,930 with EtOH in the posterior VTA significantly reduced the effects of EtOH on extracellular dopamine levels in the VP and mPFC.
CONCLUSIONS:
The results indicate that posterior VTA dopamine neurons projecting to the VP and mPFC are stimulated by local administration of EtOH and that the local stimulating effects of EtOH are mediated, at least in part, by 5-HT(3) receptors
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