18 research outputs found
Effect of Vitamin E Administration on Arginase Activity on the Liver and Kidneys of Testosterone-Treated and Castrated Rabbits
Sığır rumen doku arginazının bazı biyokimyasal özellikleri / Some biochemical properties of arginase in cattle rumen tissue
Sığır rumen doku arginazının bazı biyokimyasal özellikleri / Some biochemical properties of arginase in cattle rumen tissue
Changes in the Rate of Lipid Peroxidation in Plasma and Selected Blood Antioxidants before and during Pregnancy in Ewes
Pregnancy is a condition that favours oxidative stress. The aim of this study was to evaluate the oxidant and antioxidant status by measuring glutathione peroxidase (GSH-Px), catalase (CAT) activities and malondialdehyde (MDA) and glutathione (GSH) concentrations before and during pregnancy in ewes. Twelve healthy female and two healthy male Awassi sheep, aged 4-5 years, weighing approximately 50 - 55 kg each, were used in the study. The ewes’ blood samples were taken before synchronization and during pregnancy (on 25th days of each month of pregnancy). Plasma MDA concentrations in the 2nd and 3rd months of pregnancy in ewes were lower than in the 1st, 4th, 5th months of pregnancy and in the non-pregnant ewes. GSH concentrations and GSH-Px activities during pregnancy were increased (P P < 0.001). They were the lowest in the 2nd and 3rd months of pregnancy. The findings of our study show decreased CAT activities and elevated GSH concentrations and GSH-Px activities after the 1st month of pregnancy in ewes. These changes in the indicators under study may indicate a predisposition to oxidative stress in the 2nd and 3rd month of pregnancy in ewes
Comparison of Oxidant and Antioxidant Status of Çoruh trout (Salmo coruhensis), Anatolian trout (Salmo rizeensis) and Rainbow trout (Oncorynchus mykiss) Spermatozoa
The aim of present study was to compare oxidant and antioxidant status of Çoruh trout (Salmo coruhensis), Anatolian
trout (Salmo rizeensis) and rainbow trout (Oncorynchus mykiss) spermatozoa. Fish were obtained from Uzungöl. Enzymatic
antioxidant activities (superoxide dismutase, catalase, glutathione peroxidase), glutathione and lipid peroxidation
(malondialdehyde) were determined in spermatozoa of three trout species. Results indicated that catalase (23.36±0.36
K/g.protein), glutathione peroxidase (74.00±1.5 U/g.protein), glutathione (0.57±1.24 µmol/g.cell) and malondialdehyde
levels (6.55±2.01 nmol/g cell) were highest levels in Anatolian trout (S. rizeensis) spermatozoa. In conclusion, differences
among species caused alterations in the antioxidant and malondialdehyde levels
l-Arginine Improves Sperm Motility in Threatened Coruh Trout Salmo coruhensis (Teleostei: Salmonidae)
l-arginine is a versatile amino acid and precursor of nitric oxide that provides protection for preventing free radical damage. Herein, experiments were designed to clarify the effect of l-arginine supplementation on the sperm motility of a threatened trout, Coruh trout Salmo coruhensis. Activation solution was supplemented with levels of 0 mM (Control), 2 mM, 4 mM, and 8 mM l-arginine, and motility and survival of sperm cells were assessed. The selected concentrations were determined according to results of our preliminary experiments. The sperm motility rates at l-arginine concentrations of 0, 2, 4, and 8 mM were 73.33 ± 5.77%, 76.67 ± 5.75%, 80.01 ± 0.01%, and 90.00 ± 0.05%, respectively; notility durations were 21.67 ± 2.08, 32.33 ± 3.79, 32.67 ± 1.15, and 37.00 ± 6.38 s, respectively. Significant effect of l-arginine supplementation was determined on the percentage and duration of motile spermatozoa (p < 0.05). The maximum increment was evoked at the greatest concentration (8 mM). Here, we showed that l-arginine supplement can improve sperm motility of S. coruhensis
Malathion-induced spermatozoal oxidative damage and alterations in sperm quality of endangered trout Salmo coruhensis.
The use of pesticides has been increased along with increasing the farming activities and has caused environmental impacts deleteriously. In particular, non-target organisms including fish can be affected by toxic effects of pesticides. Therefore, the impacts of malathion (MTN) on oxidative stress and sperm quality were investigated in vitro. The MTN concentrations used on this study were 0 (control), 75, 100, and 125 μg/L. Lipid peroxidation (MDA), non-enzymatic (GSH), and enzymatic (SOD, GSH-Px, and CAT) activities in spermatozoa were examined for determination of oxidative stress status. Our findings showed that motility rate and period of sperm cells significantly decreased with exposure to MTN. Biochemical assays revealed that CAT activity and levels of MDA, GSH increased in spermatozoa based on concentration while activity of GSH-Px and SOD decreased. Consequently, spermatozoa were highly sensitive to MTN exposure. MTN has disruptive effects on sperm quality and caused to oxidative stress in spermatozoa
Effect of the organophosphate insecticidechlorpyrifos exposure on oxidative stress andquality of Salmo coruhensis spermatozoa
The use of pesticides has been increasing along with increasing farming activities and has caused deleterious environmental impacts. Non-target organisms in particular, including fish, are affected by pesticides. In this work, the impacts of Chlorpyrifos (CPF) on sperm oxidative stress markers and sperm motility were investigated in vitro. CPF concentrations were 0 μg/L (control), 5 μg/L, 10 μg/L and 15 μg/L. Lipid peroxidation [malondialdehyde (MDA)], nonenzymatic antioxidants [glutathione (GSH)] and enzymatic [superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT)] activities in sperm cells were examined for the determination of oxidative stress status. Our findings showed that motility and survival of sperm cells significantly decreased with exposure to Chlorpyrifos. Biochemical assays revealed that CAT activity and levels of MDA and, GSH increased in spermatozoa based on CPF concentration while activity of GSH-Px and SOD decreased. Consequently, spermatozoa were highly sensitive to CPF exposure. It can be deduced that CPF has the potential to disrupt sperm quality and to cause to oxidative stress in sperm cells of S. coruhensis
Cypermethrin-Induced in vitro Alterations on Oxidative Stress and Quality of Salmo coruhensis Spermatozoa
The use of insecticides has been increasing along with increasing agriculture activities
and has caused deleterious environmental impacts. Non-target organisms in particular,
including fish, are affected by pesticides. In this work, the impacts of cypermethrin
(CYP) on sperm oxidative stress markers and sperm motility were investigated in vitro.
The CYP concentrations were 0 μg L-1 control, ethanol), 1.025 μg L-1, 2.05 μg L-1 and
4.1 μg L-1. Lipid peroxidation [Malondialdehyde (MDA)], non-enzymatic antioxidants
[glutathione (GSH)] and enzymatic [superoxide dismutase (SOD), glutathione peroxidase
(GSH-Px) and catalase (CAT)] activities in sperm cells were examined for determination
of oxidative stress status. Our findings showed that motility and survival of sperm
cells significantly decreased with exposure to CYP. Biochemical assays revealed that
CAT activity and levels of MDA and, GSH increased in spermatozoa based on CYP
concentration while activity of GSH-Px and SOD decreased. Consequently, spermatozoa
were highly sensitive to CYP exposure. It can be deduced that CYP has the potential to
disrupt sperm quality and to cause to oxidative stress in sperm cells of S. coruhensis