30 research outputs found

    Izolacija iz periferne krvi i analiza funkcija heterofila kokoši protočnom citometrijom – metodološko istraživanje

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    The aim of this study was to modify the flow cytometric method, which is used for analyzing neutrophils, to analyse the functions of avian heterophils. The blood samples used in the experiments were obtained from hens in slaughterhouses. Blood samples were collected from 10 hens for each trial. Within the scope of the present study, trials were carried out regarding the amount of blood, cell suspension, dihydrorodamine-123 (DHR-123), phorbol-12-myristate-13-acetate (PMA), and N-formyl-methionyl-leucyl-phenylalanine (fMLP), as well as the storage duration of blood samples and incubation time. The results showed that 0.5-3 ml of blood could be used to detect heterophil functions, and it would be ideal to conduct analyses using fresh blood samples. In addition, the results showed that blood stored at +4 °C for up to 8 hours may be also used if necessary. In order to isolate the cells, centrifugation for 30 minutes is sufficient, and it is appropriate to use a 30μL cell suspension. 2μL of DHR-123 should be used as a chemical probe to measure heterophil functions. Excessive use of DHR-123 affected the heterophil functions negatively. In addition, it was observed that using 2μL of fMLP, which is used as an oxidative burst stimulant, and 2μL of PMA as a stimulant of chemotaxic activity, were sufficient. It was concluded that incubation at 41 °C for 5 minutes after stimulating the heterophils is also sufficient. We conclude that the methods established in this study could be used to isolate heterophils and to analyze them by flow cytometry. Therefore, this study would contribute to further research and clinical studies in poultry.Cilj je istraživanja bio prilagoditi metodu protočne citometrije, koja se inače primjenjuje u analizi neutrofila, za analizu funkcija ptičjih heterofila. Uzorci krvi 10 kokoši dobiveni su iz klaonica. Uzevši u obzir količinu krvi, kao i trajanje pohrane krvnih uzoraka odnosno vrijeme inkubacije, analizirana su stanična suspenzija, dihidrorodamin-123 (DHR-123), forbol-12-miristat-13-acetat (PMA) i N-formil-metionil-leucil-fenilalanin (fMLP). Rezultati su pokazali da se 0,5 – 3 mL krvi može upotrijebiti za otkrivanje funkcija heterofila te da bi idealno bilo analizirati svježe uzorke krvi. Osim toga rezultati su pokazali da se i krv pohranjena na temperaturi od +4 °C, u vremenu do 8 sati, može upotrijebiti ako je to potrebno. Kako bi se stanice izolirale, dovoljno je centrifugirati 30 minuta uz primjenu stanične suspenzije od 30 μL. Kao kemijsku probu za mjerenje funkcija heterofila trebalo bi upotrijebiti 2 μL DHR-123. Prekomjerna upotreba DHR-123 negativno je utjecala na funkcije heterofila. Također, uočeno je da je bila dovoljna primjena 2 μL fMLP-a, koji služi kao stimulans oksidacijskog izgaranja, kao i primjena 2 μL PMA-a kao stimulansa kemotaktičke aktivnosti. Zaključeno je da je inkubacija na temperaturi od 41 °C, tijekom 5 minuta poslije stimulacije heterofila također dovoljna. Rezultati su pokazali da se metode ustanovljene u ovom radu mogu primijeniti za izolaciju heterofila i njihovu analizu protočnom citometrijom, čime se doprinosi daljnjim istraživanjima i kliničkim studijama u peradi

    Erzurum'dan bir aşık : Muhittin Geyik'in (Efendi) hayatı

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    Ankara : İhsan Doğramacı Bilkent Üniversitesi İktisadi, İdari ve Sosyal Bilimler Fakültesi, Tarih Bölümü, 2018.This work is a student project of the Department of History, Faculty of Economics, Administrative and Social Sciences, İhsan Doğramacı Bilkent University.The History of Turkey course (HIST200) is a requirement for all Bilkent undergraduates. It is designed to encourage students to work in groups on projects concerning any topic of their choice that relates to the history of Turkey. It is designed as an interactive course with an emphasis on research and the objective of investigating events, chronologically short historical periods, as well as historic representations. Students from all departments prepare and present final projects for examination by a committee, with 10 projects chosen to receive awards.Includes bibliographical references (page 15).by Abdürrahim Özer

    LGALS3 and AXIN1 gene variants playing role in the Wnt/beta-catenin signaling pathway are associated with mucinous component and tumor size in colorectal cancer

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    The Wnt pathway alterations have been identified in colorectal and many other cancer types. It has been reported that galectin-3 (which is encoded by the LGALS3 gene) alters the signaling mechanism in the Wnt/beta-catenin pathway by binding to beta-catenin in colon and other cancers. AXIN1 is mainly responsible for the assembly of the beta-catenin destruction complex in the Wnt pathway. This study investigated the relationship of rs4644 and rs4652 variants of the LGALS3 gene and rs214250 variants of the AXIN1 gene to histopathological and clinical properties. Our study included a total of 236 patients, of whom 119 had colorectal cancer (42 women, 77 men) and 117 were healthy controls. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and allele-specific oligonucleotide (ASO) PCR methods were used. In addition, the serum galectin-3 level was studied with the enzyme-linked immunosorbent assay (ELISA) method. For the rs4644 variant of the LGALS(3) gene, the CC genotype a mucinous component was significantly more common than those without a mucinous component (p=0.026). C allele frequency of the rs214250 variant of the AXIN1 gene was significantly correlated to tumor size in the advanced tumor stage (p=0.022). The CCAACT haplotype was more common in colorectal cancer patients (p=0.022). Serum galectin-3 level was higher in the patient group compared to the control group (5.9 +/- 0.69 ng/ml vs. 0.79 +/- 0.01 ng/ml; p<0.001). In conclusion, variants of LGALS3 and AXIN1 genes affect tumor sizes and the mucinous component via Wnt/beta-catenin pathway in the pathogenesis of colorectal cancer
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