Automated access to well-defined ionic oligosaccharides

Ionic polysaccharides are part of many biological events, but lack structural characterisation due to challenging purifications and complex synthesis. Four monosaccharides bearing modifications not found in nature are used for the automated synthesis of a collection of ionic oligosaccharides. Structural analysis reveals how the charge pattern affects glycan conformation

Anticardiolipin autoantibodies in serum samples and cryoglobulins of patients with chronic hepatitis C infection

OBJECTIVE—Chronic hepatitis C virus (HCV) has been linked to extrahepatic autoimmune phenomena. In addition, a variety of autoantibodies are found in patients with HCV. The prevalence, nature, and clinical significance of anticardiolipin (aCL) autoantibodies in serum samples of patients with HCV were therefore investigated.
PATIENTS AND METHODS—A prospective study of 48 consecutive patients with chronic HCV with no evidence of previous hepatitis B virus (HBV) infection or any other autoimmune disorder. Thirty patients with HBV and 50 healthy volunteers matched for age and sex served as control groups. Anticardiolipin antibodies in the serum samples and cryoprecipitates were measured by a sensitive enzyme linked immunosorbent assay (ELISA). The β(2) glycoprotein I (β(2)-GPI) dependency was determined by carrying out aCL assays in the presence or absence of fetal calf serum samples.
RESULTS—High levels of IgG aCL antibodies were detected in serum samples of 21/48 (44%) patients with HCV. These autoantibodies showed no β(2)-GPI dependency. The control groups had much lower levels of aCL antibodies (20% in the patients with HBV and none in the normal volunteers). Cryoprecipitates from four patients with HCV (three aCL positive; one aCL negative) were further isolated. In two of the three aCL positive patients, specific cardiolipin reactivity was shown in the cryoprecipitates. The group of patients with HCV and aCL antibodies in their serum showed significantly higher total IgG levels, a higher incidence of antinuclear antibodies, and viraemia (HCV RNA) than the aCL negative patients. None of the patients with HCV and aCL antibodies showed any clinical manifestations related to those autoantibodies.
CONCLUSIONS—This study clearly shows a high prevalence of IgG aCL antibodies in the serum of patients with HCV and the localisation of these antibodies in some cryoprecipitates. The role of these autoantibodies on the course of HCV infection and their clinical significance has not yet been determined.


New visions, old practices: policy and regulation in the internet era

Raymond Williams’ comment applies as much to the media and communication systems of his time as it does to today’s Internet era. As Silverstone (2007: 26) wrote, “mediated connection and interconnection define the dominant infrastructure for the conduct of social, political and economic life across the globe”. The Internet is no more a neutral configuration of technologies than was the earlier media and communication system. If there are forces that are shaping the Internet’s development in ways that are not equitable then there is a case for countering them. This paper offers an assessment of current trends in policy and regulation that bear on the Internet. The aim is to discern whether visions of a post-neoliberal period are visible in policy and regulatory practice in this area. Though some argue that developments in Internet governance are beginning to wrest control of the Internet away from state or private sector influence, I suggest that this is a very one-sided view. In this paper, I argue that the forces influencing Internet developments are not benign because an unregulated Internet is unlikely to maximise the benefits of the Internet for all. This paper focuses on corporate interests in the Internet’s evolution and on the state’s role in regulating various components of the infrastructure and services that employ the Internet. The following section considers the paradoxical alliance between the neoliberal agenda and the advocates of the open unregulated Internet. The impact of the neoliberal agenda on the telecommunication, broadcast and Internet segments of the media and communication industry is then considered briefly, providing a basis for a more in-depth consideration of the incentives encouraging corporate actors to engage in monopolisation strategies as a means of maximising their profits. In the penultimate section, the likelihood of a shift to policy based on a post-neoliberal paradigm is explored through an examination of some recent developments in network infrastructure, broadcast content and radio frequency spectrum policy