21 research outputs found
The Cosmic Origins Spectrograph
The Cosmic Origins Spectrograph (COS) is a moderate-resolution spectrograph
with unprecedented sensitivity that was installed into the Hubble Space
Telescope (HST) in May 2009, during HST Servicing Mission 4 (STS-125). We
present the design philosophy and summarize the key characteristics of the
instrument that will be of interest to potential observers. For faint targets,
with flux F_lambda ~ 1.0E10-14 ergs/s/cm2/Angstrom, COS can achieve comparable
signal to noise (when compared to STIS echelle modes) in 1-2% of the observing
time. This has led to a significant increase in the total data volume and data
quality available to the community. For example, in the first 20 months of
science operation (September 2009 - June 2011) the cumulative redshift
pathlength of extragalactic sight lines sampled by COS is 9 times that sampled
at moderate resolution in 19 previous years of Hubble observations. COS
programs have observed 214 distinct lines of sight suitable for study of the
intergalactic medium as of June 2011. COS has measured, for the first time with
high reliability, broad Lya absorbers and Ne VIII in the intergalactic medium,
and observed the HeII reionization epoch along multiple sightlines. COS has
detected the first CO emission and absorption in the UV spectra of low-mass
circumstellar disks at the epoch of giant planet formation, and detected
multiple ionization states of metals in extra-solar planetary atmospheres. In
the coming years, COS will continue its census of intergalactic gas, probe
galactic and cosmic structure, and explore physics in our solar system and
Galaxy.Comment: 17 pages, 15 figure
Genome-Scale Reconstruction and Analysis of the Pseudomonas putida KT2440 Metabolic Network Facilitates Applications in Biotechnology
A cornerstone of biotechnology is the use of microorganisms for the efficient
production of chemicals and the elimination of harmful waste.
Pseudomonas putida is an archetype of such microbes due to
its metabolic versatility, stress resistance, amenability to genetic
modifications, and vast potential for environmental and industrial applications.
To address both the elucidation of the metabolic wiring in P.
putida and its uses in biocatalysis, in particular for the production
of non-growth-related biochemicals, we developed and present here a genome-scale
constraint-based model of the metabolism of P. putida KT2440.
Network reconstruction and flux balance analysis (FBA) enabled definition of the
structure of the metabolic network, identification of knowledge gaps, and
pin-pointing of essential metabolic functions, facilitating thereby the
refinement of gene annotations. FBA and flux variability analysis were used to
analyze the properties, potential, and limits of the model. These analyses
allowed identification, under various conditions, of key features of metabolism
such as growth yield, resource distribution, network robustness, and gene
essentiality. The model was validated with data from continuous cell cultures,
high-throughput phenotyping data, 13C-measurement of internal flux
distributions, and specifically generated knock-out mutants. Auxotrophy was
correctly predicted in 75% of the cases. These systematic analyses
revealed that the metabolic network structure is the main factor determining the
accuracy of predictions, whereas biomass composition has negligible influence.
Finally, we drew on the model to devise metabolic engineering strategies to
improve production of polyhydroxyalkanoates, a class of biotechnologically
useful compounds whose synthesis is not coupled to cell survival. The solidly
validated model yields valuable insights into genotype–phenotype
relationships and provides a sound framework to explore this versatile bacterium
and to capitalize on its vast biotechnological potential
Pharmacotherapy of methamphetamine addiction: an update
Abstract Methamphetamine dependence is a serious public health problem worldwide for which there are no approved pharmacological treatments. Psychotherapy is still the mainstay of treatment; however, relapse rates are high. The search for effective pharmacological treatment has intensified in the last decade. This review will highlight progress in pharmacological interventions to treat methamphetamine dependence as well as explore new pharmacological targets. Published data from clinical trials for stimulant addiction were searched using PubMed and summarized, as well as highlights from a recent symposium on methamphetamine pharmacotherapy presented at the ISAM 2006 meeting, including interim analysis data from an ongoing D-amphetamine study in Australia. Early pilot data are encouraging for administering D-amphetamine and methylphenidate as treatment for heavy amphetamine users. Abilify at 15 mg/day dose increased amphetamine use in an outpatient pilot study. Sertraline, ondansetron, baclofen, tyrosine, and imipramine were ineffective in proof-of-concept studies. Development of pharmacotherapy for methamphetamine dependence is still in an early stage. Data suggesting D-amphetamine and methylphenidate as effective pharmacotherapy for methamphetamine addiction will need to be confirmed by larger trials. Preclinical data suggest that use of GVG, CB1 antagonist, and lobeline are also promising therapeutic strategies.Ahmed Elkashef, Frank Vocci, Glen Hanson, Jason White, Wendy Wickes and Jari Tiihone