Investigation of strains of Tomato yellow leaf curl virus (TYLCV) in Çukurova Region.

TEZ11191Tez (Yüksek Lisans) -- Çukurova Üniversitesi, Adana, 2018.Kaynakça (s. 55-65) var.xvii, 65 s. : res. (bzs. rnk.), tablo ; 29 cm.Tomato yellow curl virus (TYLCV) örtüaltı domates yetiştiriciliğinin yapıldığı alanlarda domateste (Lycopersicum esculentum) önemli ekonomik kayıplara neden olan bir viral etmendir. TYLCV ile bulaşık bitkilerde gelişme geriliği ile beraber yapraklarda kıvırcıklık, küçülme ve sararma gibi simptomlar görülmektedir. Birçok ırkı rapor edilen TYLCV’nin ırklarının simptomatolojik olarak ayırt edilmesi mümkün değildir. Bu çalışma, 2017 yılında Adana ve Mersin illerinde TYLCV’nin ırklarının araştırılması amacıyla gerçekleştirilmiş. Yaprak örnekleri TYLCV ile bulaşık bitkilerden toplanmış ve TYLCV’nin varlığı saptanmıştır. Bu çalışmada kullanılan TYLCV ırklarına spesifik primer çiftleri (TYLCV-IL , TYLCV-Mld) ile iki ırkı tespit edilmiştir. Aynı simptomları gösteren fakat ırklara spesifik primer çiftlerine bant vermeyen izolatlar, V1 kılıf protein bölgesini amplifiye eden (777 bp) primer çiftiyle testlenmiştir. Filogenetik analizler sonucunda, TYLCV-IL ve TYLCV-Mild’e bant veren izolatlar farklı ülkelerde rapor edilmiş bu ırklar ile yaklaşık %99 benzerlik göstermiştir. Ancak Mersin’den alınan iki izolatın, 777 bp büyüklüğündeki kılıf proteinin filogenetik analizinin sonucunda, dünya genelinde rapor edilmiş birçok TYLCV ırkı ve izolatıyla benzerliği olduğu tespit edilmiştir. Bu sonuçlar ışığında, TYLCV’nin birçok viral varyantının Akdeniz Bölgesi’nde örtüaltında gerçekleştirilen domates yetiştiriciliğini tehdit ettiği ortaya konmuştur.Tomato yellow leaf curl virus (TYLCV) is a viral agent causes significant economic loss worldwide on tomato (Lycopersicum esculentum) cultivation in greenhouses. The symptoms caused by TYLCV at infected tomato plants are stunting, small chlorotic leaves with upward curling. Many Different strains of TYLCV have been reported from various parts of the World and it’s impossible to discriminate the strains by symptoms. This study was carried out for identification and characterization of TYLCV strains in Adana and Mersin provinces (Cukurova region) of Turkey in 2017. With this aim, leaf samples were collected from infected tomato plants and presence of TYLCV was confirmed by PCR. Two different strains of TYLCV (TYLCV-IL, TYLCV-Mld) were identified by using strain specific primers (TYLCV-IL, TYLCV-Mld) in Cukurova region. Similar symptomatic plants were tested with V1 primers amplifying the coat protein (CP) coding gene – 777 bp which had no PCR products for both TYLCV-IL and TYLCV-Mld specific primers. Phylogenetic analysis of TYLCV-IL and TYLCV-Mld proved that these isolates were related to other isolates from several countries. Although, phylogenetic analysis of CP (777 bp) of two isolates from Mersin province had similarities with most of the TYLCV strains and isolates from various countries all around the World. These results showed that TYLCV had many viral variants being a threat for tomato cultivation in greenhouses in the Mediterranean region of Turkey.Bu çalışma Ç.Ü. Bilimsel Araştırma Projeleri Birimi tarafından desteklenmiştir. Proje No: FYL-2017-7747

Yüksek doz oral fluoksetin alımı sonrası gelişen oküler herpes simpleks virus aktivasyonu

A 51-year-old man with high intraocular pressure on the left eye was referred to our clinic. A laser iridotomy was performed with full anti-glaucoma medication prior to the referral. There was a fixed dilated irregular pupil of the left eye, accompanied with mild corneal edema, a paracentral stromal corneal haze, patchy iris atrophy, fine keratic precipitates, trace amounts of cells and pigments in the anterior chamber and a patent iridotomy. Medical history was revealed a previous herpetic episode 7 years ago and fluoxetine use for major depression for 2 years which he overdosed 5 days before his ocular symptoms have started. Ocular herpes simplex virus activation associated with high dose fluoxetine was suspected. Fluoxetine was discontinued. Oral acyclovir, topical steroids and anti-glaucoma medication has been prescribed. A week later, on his control visit, the intraocular pressure was normalized and clinical findings have subsided. Fluoxetine, a selective serotonin re-uptake inhibitor, and some other anti-depressants, has been proved to suppress cellular immunity. Herpes simplex virus activation after surreptitious self-administration of high dose fluoxetine in this case is much more probable than coincidence. This is the first reported case of ocular herpes activation related to fluoxetine use.Yüksek Doz Oral Fluoksetin Alımı Sonrası Gelişen Oküler Herpes Simpleks Virus AktivasyonuElli bir yaşında erkek hasta, kliniğimize yüksek göz içi basıncı nedeniyle refere edilmiş. Öncesinde hastaya tam antiglokomatöz tedavi ve lazer iridotomi uygulanmış. Hastanın sol gözünde fikse dilate düzensiz pupilla ve eşlik eden hafif kornea ödemi, parasantral stromal korneal bulanıklık, yama şeklinde iris atrofisi, keratik presipitatlar, ön kamarada eser miktarda hücre ve pigment kümeleri ve açık iridotomi izlendi. Medikal öyküsünde 7 yıl önce geçirilmiş herpetik atak öyküsü belirlendi ve major depresyon nedeniyle 2 yıldır fluoksetin kullanan hastanın göz yakınmalarının başlamasından 5 gün öncesinde ilacı yüksek dozda aldığı belirlendi. Oküler herpes simpleks virus aktivasyonunun yüksek doz fluoksetin alımından olabileceğinden şüphelenildi. Fluoksetin kesildi. Oral asiklovir, topikal steroid ve anti-glokomatöz tedavi başlandı. Bir hafta sonra, hastanın kontrol muayenesinde göz içi basıncının normale geldiği ve klinik bulguların yatışmış olduğu izlendi. Fluoksetin, selektif serotonin geri alım inhibitörü olup, bazı diğer antidepresan ilaçlar gibi, hücresel immüniteyi baskıladığı kanıtlanmıştır. Bu olguda olduğu gibi hastanın gizlice yüksek doz fluoksetin alımı sonrası gelişen herpes simpleks virus aktivasyonu tesadüf olmasından daha olasıdır. Fluoksetin kullanımıyla ilişkili oküler herpes aktivasyonu olgusu ilk kez rapor edilmektedir

Pseudoexfoliation syndrome and antidepressant drug use

*Erdoğan, Yaşar ( Aksaray, Yazar )Objectives: To investigate the relationship between pseudoexfoliation syndrome (PES) and the use of antidepressant medications. Materials and Methods: This population-based, cross-sectional study included 2,017 of 2,356 invited subjects who were randomly selected from the Turkish Statistical Institute database (www.turksat.gov.tr/) as part of an epidemiologic study which specifically aimed to detect the prevalence of PES in the province of Eskişehir. During the examination, a detailed questionnaire was administered to determine physician-diagnosed systemic disease and drug use. Results: Eight of the 2,017 participants in the study were excluded for various reasons (2 with posterior synechiae, 2 with corneal opacity, 1 uncooperative patient, 1 eviscerated patient, 1 with active adenoviral keratoconjunctivitis, and 1 with a history of angle closure). PES was detected in 100 (5%) of the 2,009 patients whose data were analyzed. The percentage of subjects with concurrent antidepressant drug use in the PES and non-PES non-glaucoma groups were 4.1% (n=3) and 1.1% (n=21), respectively. The difference between the two groups was statistically significant (p=0.024). In addition, the use of medications for hypertension (p<0.001) and coronary artery disease (p=0.009) was also higher in the PES group. Conclusion: The higher prevalence of antidepressant drug use in patients with PES may be related to the processes of vascular damage and inflammation common to the pathogenesis of both PES and depression, as well as the high rate of chronic systemic comorbidities in these patients...

High Intraocular Pressure After Carbamazepine and Gabapentin Intake in a Pseudoexfoliative Patient

Introduction: Psychotropic agents may frequently be associated with ocular adverse effects, which include angle-closure glaucoma. We report a case of pseudoexfoliation glaucoma in which intraocular pressure (IOP) increased within hours after carbamazepine and gabapentin intake, with no observable evidence of any angle closure

Treatment of phthiriasis palpebrarum and crab louse: Petrolatum jelly and 1% permethrin shampoo

WOS: 000215278400032PubMed ID: 26451147Phthiriasis palpebrarum is an uncommon cause of blepharoconjunctivitis in which Pthirus pubis infest the eyelashes. We report a case of unilateral phthiriasis palpebrarum with crab louse. A 45-year-old man presented with conjunctival hyperaemia and moderate itching associated with irritation, and crusty excretions of the eyelashes in the left eye. Careful slit-lamp examination revealed many lice and nits in left eye and mild conjunctival hyperaemia. No abnormality was found in the right eye. On dermatologic examination, only one louse was found at the pubic area. The patient was treated effectively with petrolatum jelly (Vaseline) and 1% permethrin shampoo (Kwellada 1% shampoo). At the end of the firstweek no louse or nitwas present on eyelashes and pubic area

Comparison of Different Types of Complications in the Phacoemulsification Surgery Learning Curve According to Number of Operations Performed

Objectives: To compare the differences in intraoperative complications rates by the number of resident-performed sequential phacoemulsification surgeries. Materials and Methods: Preoperative and postoperative ophthalmological examination records and intraoperative data of 180 eyes of 140 patients who underwent cataract surgery by two residents between November 2009 and February 2012 were analyzed retrospectively. The data of 180 eyes were separated into 3 groups based on the number of operations performed: Group A (first 1-60 eyes), group B (61- 120 eyes) and group C (last 121-180 eyes). The number of direct supervisor interventions and the rates of different types of complications were compared between the three groups. Results: The number of direct supervisor interventions was 45, 35 and 19 in group A, B and C, respectively. The number of complications anterior to the iris plane was 3, 4 and 12 in group A, B and C, respectively. The difference in the rate of complications between group B and C was statistically significant (p=0.029). The number of complications posterior to the iris plane was 6, 14 and 3 in group A, B and C, respectively. The difference in the rate of complications between the groups was statistically significant (p=0.042, p=0.004). Conclusion: This study provides insight into which types of complications might arise during the phacoemulsification training period. The trends in the rates of different complication types in clinics may be analyzed, and this analysis may be used to improve and modify phacoemulsification training programmes according to the needs of residents

Four Cases of Pediatric Photokeratitis Present to the Emergency Department After Watching the Same Theater Show

We report four consecutive cases of photokeratitis that presented to the emergency department, interestingly after having watched the same theatre performance in the same school. The patients’ ages (3 male, 1 female) ranged from 9 to 13 years. All patients presented with similar complaints consisting of pain, tearing, foreign body sensation, photophobia and blurred vision in both eyes. Patients reported watching a theatre performance in the same school approximately 4 hours before symptom onset. On slit-lamp examination, conjunctival injection and corneal punctate epithelial erosions were observed in the interpalpebral zone in both eyes. On fundus examination, no pathology was observed in the vitreous, posterior pole or peripheral retina. All cases were treated with topical antibiotics and lubricant eye drops. Corneas were clear two days later in the control visit. In this case report, exposure to ultraviolet light from high-power lamps used in the theatre was proposed as a possible cause of corneal epithelial cell damage and subsequent photokeratitis. (Turk J Ophthalmol 2015; 45: 226-228