1,031 research outputs found
Peptide Mass Spectra from Micrometer-Thick Ice Films Produced with Femtosecond Pulses
We present a cryogenic mass spectrometry protocol with the capability to detect peptides in the attomole dilution range from ice films. Our approach employs femtosecond laser pulses and implements neither substrate modification nor proton donor agents in the aqueous solution, known to facilitate analyte detection in mass spectrometry. In a systematic study, we investigated the impact of temperature, substrate composition, and irradiation wavelength (513 and 1026 nm) on the bradykinin signal onset. Our findings show that substrate choice and irradiation wavelength have a minor impact on signal intensity once the preparation protocol is optimized. However, if the temperature is increased from â140 to 0 °C, which is accompanied by ice film thinning, a somehow complex picture of analyte desorption and ionization is recognizable, which has not been described in the literature yet. Under cryogenic conditions (â140 °C), obtaining a signal is only possible from isolated sweet spots across the film. If the thin ice film is between â100 and â70 °C of temperature, these sweet spots appear more frequently. Ice sublimation triggered by temperatures above â70 °C leads to an intense and robust signal onset that could be maintained for several hours. In addition to the above findings, we notice that a vibrant fragmentation pattern produced is strikingly similar with both wavelengths. Our findings suggest that while following an optimized protocol, femtosecond mass spectrometry has excellent potential to analyze small organic molecules and peptides with a mass range of up to 2.5 kDa in aqueous solution without any matrix, as employed in matrix-assisted laser desorption/ionization (MALDI) or any substrate surface modification, found in surface-assisted laser desorption/ionization (SALDI)
The HARE chip for efficient time-resolved serial synchrotron crystallography
Serial synchrotron crystallography (SSX) is an emerging technique for static and time-resolved protein structure determination. Using specifically patterned silicon chips for sample delivery, the `hit-and-return' (HARE) protocol allows for efficient time-resolved data collection. The specific pattern of the crystal wells in the HARE chip provides direct access to many discrete time points. HARE chips allow for optical excitation as well as on-chip mixing for reaction initiation, making a large number of protein systems amenable to time-resolved studies. Loading of protein microcrystals onto the HARE chip is streamlined by a novel vacuum loading platform that allows fine-tuning of suction strength while maintaining a humid environment to prevent crystal dehydration. To enable the widespread use of time-resolved serial synchrotron crystallography (TR-SSX), detailed technical descriptions of a set of accessories that facilitate TR-SSX workflows are provided
Imaging Molecular Structure through Femtosecond Photoelectron Diffraction on Aligned and Oriented Gas-Phase Molecules
This paper gives an account of our progress towards performing femtosecond
time-resolved photoelectron diffraction on gas-phase molecules in a pump-probe
setup combining optical lasers and an X-ray Free-Electron Laser. We present
results of two experiments aimed at measuring photoelectron angular
distributions of laser-aligned 1-ethynyl-4-fluorobenzene (C8H5F) and
dissociating, laseraligned 1,4-dibromobenzene (C6H4Br2) molecules and discuss
them in the larger context of photoelectron diffraction on gas-phase molecules.
We also show how the strong nanosecond laser pulse used for adiabatically
laser-aligning the molecules influences the measured electron and ion spectra
and angular distributions, and discuss how this may affect the outcome of
future time-resolved photoelectron diffraction experiments.Comment: 24 pages, 10 figures, Faraday Discussions 17
Feynman Graphs and Generalized Eikonal Approach to High Energy Knock-Out Processes
The cross section of hard semi-exclusive reactions for fixed
missing energy and momentum is calculated within the eikonal approximation.
Relativistic dynamics and kinematics of high energy processes are unambiguously
accounted for by using the analysis of appropriate Feynman diagrams. A
significant dependence of the final state interactions on the missing energy is
found, which is important for interpretation of forthcoming color transparency
experiments. A new, more stringent kinematic restriction on the region where
the contribution of short-range nucleon correlations is enhanced in
semi-exclusive knock-out processes is derived. It is also demonstrated that the
use of light-cone variables leads to a considerable simplification of the
description of high-energy knock-out reactions.Comment: 24 pages, LaTex, two Latex and two ps figures, uses FEYNMAN.tex and
psfig.sty. Revisied version to appear in Phys. Rev.
Bottom Production
We review the prospects for bottom production physics at the LHC.Comment: 74 pages, Latex, 71 figures, to appear in the Report of the ``1999
CERN Workshop on SM physics (and more) at the LHC'', P. Nason, G. Ridolfi, O.
Schneider G.F. Tartarelli, P. Vikas (conveners
Sur la p-dimension des corps
Let A be an excellent integral henselian local noetherian ring, k its residue
field of characteristic p>0 and K its fraction field. Using an algebraization
technique introduced by the first named author, and the one-dimension case
already proved by Kazuya KATO, we prove the following formula: cd_p(K) = dim(A)
+ p-rank(k), if k is separably closed and K of characteristic zero. A similar
statement is valid without those assumptions on k and K
Model Calculations for the Two-Fragment Electro-Disintegration of He
Differential cross sections for the electro-disintegration process are calculated, using a model in which
the final state interaction is included by means of a nucleon-nucleus (3+1)
potential constructed via Marchenko inversion. The required bound-state wave
functions are calculated within the integrodifferential equation approach
(IDEA). In our model the important condition that the initial bound state and
the final scattering state are orthogonal is fulfilled. The sensitivity of the
cross section to the input interaction in certain kinematical regions
is investigated. The approach adopted could be useful in reactions involving
few cluster systems where effective interactions are not well known and exact
methods are presently unavailable. Although, our Plane-Wave Impulse
Approximation results exhibit, similarly to other calculations, a dip in the
five-fold differential cross-section around a missing momentum of , it is argued that this is an artifact of the omission of re-scattering
four-nucleon processes.Comment: 16 pages, 6 figures, accepted for publication by Phys.Rev.
Inclusive pion production in 360 GeV/cpp interactions
In a study of proton-proton interactions at âs=26GeV, inclusive distributions of single pions and systems of pions of various charge combinations are presented, as well as the production ratio obtained in association with various trigger particles. The results are compared to current phenomenological models in an attempt to understand multiparticle productions in hadron collisions
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