Gamma-Ray Bursts from Primordial Quark Objects in Space

We investigate the possibility that gamma-ray bursts originate in a concentric spherical shell with a given average redshift and find that this is indeed compatible with the data from the third BATSE (3B) catalog. It is also shown that there is enough freedom in the choice of unknown burst properties to allow even for extremely large distances to the majority of bursts. Therefore, we speculate about an early, and very energetic, origin of bursts, and suggest that they come from phase transitions in massive objects of pure quark matter, left over from the Big Bang.Comment: 11 pages, Latex, 3 postscript figures, to be publ in the Proc of the Joint Meeting of the Networks 'The Fundamental Structure of Matter' and 'Tests of the Electroweak Symmetry Breaking', Ouranoupolis, Greece, May 199

Controlled In Meso Phase Crystallization – A Method for the Structural Investigation of Membrane Proteins

We investigated in meso crystallization of membrane proteins to develop a fast screening technology which combines features of the well established classical vapor diffusion experiment with the batch meso phase crystallization, but without premixing of protein and monoolein. It inherits the advantages of both methods, namely (i) the stabilization of membrane proteins in the meso phase, (ii) the control of hydration level and additive concentration by vapor diffusion. The new technology (iii) significantly simplifies in meso crystallization experiments and allows the use of standard liquid handling robots suitable for 96 well formats. CIMP crystallization furthermore allows (iv) direct monitoring of phase transformation and crystallization events. Bacteriorhodopsin (BR) crystals of high quality and diffraction up to 1.3 Å resolution have been obtained in this approach. CIMP and the developed consumables and protocols have been successfully applied to obtain crystals of sensory rhodopsin II (SRII) from Halobacterium salinarum for the first time

Identification of epigenetically regulated genes that predict patient outcome in neuroblastoma

<p>Abstract</p> <p>Background</p> <p>Epigenetic mechanisms such as DNA methylation and histone modifications are important regulators of gene expression and are frequently involved in silencing tumor suppressor genes.</p> <p>Methods</p> <p>In order to identify genes that are epigenetically regulated in neuroblastoma tumors, we treated four neuroblastoma cell lines with the demethylating agent 5-Aza-2'-deoxycytidine (5-Aza-dC) either separately or in conjunction with the histone deacetylase inhibitor trichostatin A (TSA). Expression was analyzed using whole-genome expression arrays to identify genes activated by the treatment. These data were then combined with data from genome-wide DNA methylation arrays to identify candidate genes silenced in neuroblastoma due to DNA methylation.</p> <p>Results</p> <p>We present eight genes (<it>KRT19</it>, <it>PRKCDBP</it>, <it>SCNN1A</it>, <it>POU2F2</it>, <it>TGFBI</it>, <it>COL1A2</it>, <it>DHRS3 </it>and <it>DUSP23</it>) that are methylated in neuroblastoma, most of them not previously reported as such, some of which also distinguish between biological subsets of neuroblastoma tumors. Differential methylation was observed for the genes <it>SCNN1A </it>(p < 0.001), <it>PRKCDBP </it>(p < 0.001) and <it>KRT19 </it>(p < 0.01). Among these, the mRNA expression of <it>KRT19 </it>and <it>PRKCDBP </it>was significantly lower in patients that have died from the disease compared with patients with no evidence of disease (fold change -8.3, p = 0.01 for <it>KRT19 </it>and fold change -2.4, p = 0.04 for <it>PRKCDBP</it>).</p> <p>Conclusions</p> <p>In our study, a low methylation frequency of <it>SCNN1A</it>, <it>PRKCDBP </it>and <it>KRT19 </it>is significantly associated with favorable outcome in neuroblastoma. It is likely that analysis of specific DNA methylation will be one of several methods in future patient therapy stratification protocols for treatment of childhood neuroblastomas.</p

The impact of work on the night blood pressure dipping profile.

Objective. The purpose of this study was to elucidate whether ambulatory blood pressure (ABPM) performed during a work day and a non-work day had any impact on the night dipping profile. Study design. A crossover randomized ABPM study in primary healthcare was retrospectively analysed for the occurrence of non-dipping (ND), dipping (D) or extreme (XD) nightly dipping. Non-dippers were defined as subjects with less than 10% and extreme dippers as subjects with more than 20% nightly blood pressure fall measured as mean arterial pressure (MAP). Subjects. Forty treated hypertensives and 40 normotensives (20 men and 20 women in each group), who had performed ABPM twice in a fortnight. They had been randomly allocated to perform a work day or a non-work day as the first period. Result. Only one of the 16 subjects who at any time was a non-dipper remained so during both monitoring periods. Extreme dipping was more often reproduced in nine persons out of 29. Of all 80 subjects, 43.8% (35 persons) remained dippers during both periods. No one changed from a non-dipper to an extreme dipper or the reverse. The odds of being an ND were 3.8 times more common on a non-work day, p = 0.010. XDs were slightly more common (1.7 times) on a work day than on a non-work day, p = 0.040. There was no correlation as to the degree of MAP and the dipping profile, p = 0.629. Conclusions. More subjects were non-dippers at the end than at the beginning of the work week. It is essential to consider this when attempting to identify a non-dipper by ABPM

Interrelationships between childbearing and housing transitions in the family life course

Research has examined the effect of family changes on housing transitions and childbearing patterns within various housing types. Although most research has investigated how an event in one domain of family life depends on the current state in another domain, the interplay between them has been little studied. This study examines the interrelationships between childbearing decisions and housing transitions. We use rich longitudinal register data from Finland and apply multilevel event history analysis to allow for multiple births and housing changes over the life course. We investigate the timing of fertility decisions and housing choices with respect to each other. We model childbearing and housing transitions jointly to control for time-invariant unobserved characteristics of women, which may simultaneously influence their fertility behavior and housing choices, and we show how joint modeling leads to a deeper understanding of the interplay between the two domains of family life