Korelasi antara kadar gula darah puasa dengan stress oksidatif dan aktivitas katalase pada tikus model diabetes melitus tipe 1 yang diterapi dengan MSC-WJ

Objective: This study was conducted to investigate the involvement of oxidative damage in type 1 diabetes mellitus rats treated with Mesenchymal Stem Cell Wharton's Jelly (MSC-WJ) and to explain the correlation between fasting blood sugar levels and MDA levels as an indicator of oxidative stress and catalase activity.Methods: This research is experimental research with a Posttest-Only Control Group Design. It consisted of 3 groups, namely group 1 was healthy rats, group 2 was DM rats and group 3 was DM rats treated with MSC-WJ. This study conducted in vivo experiments on alloxan-induced diabetic rat models by testing fasting blood glucose (FBG), serum MDA levels, and catalase activity. Malondialdehyde (MDA) was measured by the Ohkawa method, and catalase activity (CAT) was measured by a modified Sinha method.Results: The results showed the correlation analysis between fasting blood sugar levels and MDA levels (r = 0.697) and catalase activity (r = -0.850) in type 1 DM rats after being treated with Mesenchymal Stem Cell Wharton's Jelly.Conclusion: This study shows a strong positive correlation between fasting blood sugar levels and oxidative stress and a very strong correlation between fasting blood sugar levels and catalase activity of type 1 DM rats treated with MSC-WJ

LOGAM-LOGAM BERAT PENCEMAR LINGKUNGAN DAN EFEK TERHADAP MANUSIA

Heavy metals are the hazardous substance that produced by industrial waste, included arsenic (As), lead (Pb), mercury (Hg) and cadmium (Cd). That heavy metal could cause acute and chronic body intoxication. Health effects of heavy metal intoxication such as nervous system, kidney, liver, bone, respiratory system, reproductive system, hematopoietic system, cardiovascular, gastrointestinal tract disorders. The manifestation of the toxic effect in tissue and organ is caused by interaction between heavy metal and important cell molecules thus destruct the structure and the function of the cell at the target organ

PENGARUH PEMBERIAN MESENCHYMAL STEM CELL WHARTON’S JELLY TERHADAP KADAR MATRIX METALLOPROTEINASE-1, A DISINTEGRIN-LIKE AND METALLOPROTEINASE WITH THROMBOSPONDIN MOTIF-4, INDUCIBLE NITRIC OXIDE SYNTHASE DAN INTERLEUKIN-4 PADA OSTEOARTRITIS

ABSTRAK Pengaruh Pemberian Mesenchymal Stem Cell Wharton’s Jelly Terhadap Kadar Matrix Metalloproteinase-1, A Disintegrin-Like And Metalloproteinase With Thrombospondin Motif-4, Inducible Nitric Oxide Synthase Dan Interleukin-4 Pada Osteoartritis Penelitian Eksperimental Pada Rattus Novergicus Endrinaldi Osteoarthritis (OA) secara umum dianggap sebagai penyakit sendi degeneratif yang disebabkan oleh perubahan biomekanik dan proses penuaan. Penelitian telah mengungkapkan peran proses inflamasi dalam patogenesis OA. Penelitian ini bertujuan untuk mempelajari pengaruh Mesenchymal Stem Cell Wharton’s Jelly terhadap bioactive marker osteoartritis. Penelitian ini merupakan penelitian eksperimental dengan desain Posttest-Only Control Group Design. Sampel terdiri dari 18 ekor tikus OA sebagai kelompok kontrol dan 18 ekor tikus OA yang diterapi dengan MSC-WJ sebagai kelompok perlakuan. Induksi OA dilakukan dengan injeksi monosodium iodoacetate (MIA) ke intra-artikular lutut kanan. Pemberian MSC-WJ dilakukan pada minggu ke tiga setelah induksi MIA. Kadar MMP-1, ADAMTS-4, iNOS dan IL-4 serum tikus diukur dengan metoda ELISA. Uji statistik yang digunakan adalah uji t independen. Nilai p < 0,05 dikatakan bermakna secara statistik. Dari hasil penelitian didapatkan bahwa kadar MMP-1 serum lebih tinggi pada kelompok yang diterapi MSC-WJ dibandingkan kelompok kontrol (3.96 ± 0.81 ng/ml versus 2.63 ± 0.55 ng/ml, p 0,05). Kadar iNOS serum lebih tinggi pada kelompok yang diterapi MSC-WJ dibandingkan kelompok kontrol (28,37 ± 4,23 ng/ml versus 26,21 ± 2,79 ng/ml, p > 0,05). Kadar IL-4 serum lebih tinggi pada kelompok yang diterapi MSC-WJ dibandingkan kelompok kontrol (47,95 ± 4,88 ng/L versus 43,90 ± 4,99 ng/L, p < 0,05). Penelitian ini menyimpulkan bahwa terdapat perbedaan antara kadar MMP-1 dan IL-4 serum tikus OA lutut yang diterapi dengan MSC-WJ dan yang tidak diterapi. Tidak terdapat perbedaan antara kadar ADAMTS-4 dan iNOS serum tikus OA lutut yang diterapi dengan MSC-WJ dan yang tidak diterapi. Kata Kunci : Osteoartritis, Masenchymal Stem Cell Wharton’s Jelly, MMP-1, ADAMTS-4, iNOS dan IL-

PERUBAHAN KADAR KIO3, GARAM BERIODIUM OLEH BUMBU MASAKAN

Telah dilakukan penelitian kadar KI03 (iodium) garam yang bertujuan untuk membuktikan bahwa pemanasan serta berbagai bumbu masakan dapat merusak (mereduksi) KI03 (iodium) garam. Dalampenelitian ini dilakukan penentuan KI03 (iodium) berbagai garam yang beredar di Kota Padang dan penentuan kadar KI03 (iodium) garam yang dicampur dengan berbagai bumbu masakan, menggunakanmetoda iodometri. Hasil penelitian dari enam sampel garam beriodium yang beredar di Kota Padang menunjukan bahwa tiga sampel mempunyai kadar KI03 di atas 40 ppm sedangkan tiga sampel lainnya mempunyai kadar KI03 di bawah40 ppm. Penurunan kadar KIOs garam teijadi secara tidak bermakna (p>0,0 1 ) dalam air yang sudah dipanaskan. Tetapi penurunan kadar KI03 terjadi secara bermakna (p<0,01) pada campuran garam beriodium dengan kuah bumbu cabe, bawang merah, asam jawa, asam galuh dan kunyit yang belum dan telah dipanaskan dan campuran antara garam beriodium dengan kuah bumbu langkuas dan jahe yang telah dipanaskan. Danpenurunan kadar iodium yang paling cepat terjadi pada campuran garam beriodium dengan kuah bumbu cabe, dimana kadar KI03 garam menjadi nol ppm setelah campuran dididihkan selama 5 menit. Penggunaan garam beriodium yaitu dengan cara menambahkan garam beriodium pada makanan sebelum di konsumsi

Differences in the Effect of Giving Fe Tablets and Capsules Moringa Leaves on Hepcidin and Ferritin Levels in Anemic Pregnancy Rats

Anemia is a condition characterized by a decrease in Hemoglobin (HB), a serious global public health problem especially affecting children and pregnant women. This study aimed to determine differences in the effect of giving Fe tablets and capsules of Moringa leaves on hepcidin and ferritin levels in anaemic pregnancy rats. This research was an experimental study with a randomized post-test-only control group design. This research was conducted at the Animal House of the Faculty of Medicine, Andalas University to maintain and treat experimental animals. Ferritin and Hepcidin examinations were carried out at the biomedical laboratory of the Faculty of Medicine, Andalas University. The sample in this study were female white rats (Rattus Novergicus) which met the inclusion and exclusion criteria, the data were analyzed using One Way Anova. The results of this study indicated that the mean hepcidin levels in the Fe (P1) and Moringa leaf capsule (P2) groups were 181.902 ± 20.31 and 216.362 ± 29.90 with a p-value of 0.006 . The mean ferritin levels in the Fe(P1) and Moringa leaf capsule (P2) groups were 1.928 ± 0.263 and 2.308 ± 0.320 with a p-value of 0.004. The conclusion in this study was that there was an effect on hepcidin levels after being given Fe tablets (P1) and Moringa leaf capsules (P2) to anemic pregnant rats and there was an effect on ferritin levels after being given Fe tablets (P1) and Moringa leaf capsules (P2) to anemic pregnant rats Keyword: Anemic, Ferritin, Fe Tablets, Hepcidin, Moringa Leaf Capsule

Correlation between Oxidative Stress with Cartilage Thickness and Catalase Activity in Osteoarthritis Model Rat after MSC-WJ Treatment

Objective: This study was conducted to investigate the involvement of oxidative damage in rat model of osteoarthritis treated with Mesenchymal Stem Cell Wharton Jelly and to elucidate whether oxidative stress indicators correlate with cartilage damage and catalase activity. Methods: This research is an experimental research with Posttest-Only Control Group Design. The sample consisted of 30 OA rat divided into 5 groups. Group I was the OA rat control group, group II was the OA rat group after 4 weeks, group III was the OA rat group after 8 weeks, group IV was the OA rat group after 4 weeks of treatment with MSC-WJ and group V is the OA rat group after 8 weeks treated with MSC-WJ. Malondialdehyde were measured by Ohkawa method, Cartilage Thickness were measured by using the Betaview program and catalase activity were measured by modification of Shinha.Results: The results showed that the analysis of the Correlation between MDA with Cartilage Thickness (r= – 0.665) and CAT activity (r= – 0.666) in osteoarthritis Model Rat after Mesenchymal Stem Cell Wharton’s Jelly Treatment.Conclusion: This study demonstrated a strong negative correlation between oxidative stress and knee cartilage thickness and catalase activity of MSC-WJ treated OA rats

The Effect of Mesenchymal Stem Cell Wharton’s Jelly on Nuclear Factor Kappa Beta and Interleukin-10 Levels in Osteoarthritis Rat Model

BACKGROUND: Osteoarthritis (OA) is a degenerative joint disease in one or more joints characterized by changes in pathological structures such as cartilage, hypertrophy, and remodeling of the subchondral bone and secondary inflammation of the synovium membrane, causing changes in joint components such as cells, matrices, and molecular production. At the molecular level, an imbalance between catabolic and anabolic activities in joint cartilage results in OA. Nuclear factor kappa beta (NFκβ) is a cytokine that plays an important role in the signaling pathway of the pathogenesis of OA in causing an inflammatory reaction, whereas interleukin (IL)-10 is an anti-inflammatory cytokine that is involved in the pathogenesis of OA. AIM: This study aims to prove the influence before and after administration mesenchymal stem cells from Wharton’s jelly on the serum NFκβ and IL-10 levels in OA rat models. MATERIALS AND METHODS: This research is an experimental study with the design of post-test-only control group design. The sample consisted of 16 OA rats as a control group and 16 OA rats treated with MSC-WJ as a treatment group. OA induction is done by injection of monosodium iodoacetate (MIA) into the intra-articular right knee. Giving MSC-WJ is done in the 3rd week after MIA induction. The serum NFκβ and IL-10 levels were measured after 3 weeks treated with MSC-WJ using the ELISA method. The statistical test used is an independent t-test. p &lt; 0.05 was said to be statistically significant. RESULTS: From the research results obtained, serum levels of knee OA of rat knee OA treated with mesenchymal stem cell Wharton jelly are lower than serum NFκβ levels of knee OA of the rat that is not treated, but the difference in levels of NFκβ is not significant (p &gt; 0.05). The serum IL-10 level of rat OA of knee treated with mesenchymal stem cell Wharton jelly was higher than the serum IL-10 level of rat OA of the knee that was not treated, difference in levels of IL-10, is significant (p &lt; 0.05). CONCLUSION: This study concluded that MSC-WJ significantly decreased the serum NFκβ levels of OA rats and not significantly increased the serum IL-10 levels of OA rats

PENGARUH TIMBAL ASETAT TERHADAP AKTIVITAS ENZIM KATALASE HATI TIKUS PUTIH JANTAN

AbstrakTimbal (Pb) dapat ditemukan di berbagai media lingkungan seperti udara, air, debu dan tanah. Logam Pb atau bentuk persenyawaannya berasal dari pembakaran bahan bakar kendaraan bermotor, emisi industri dan dari penggunaan cat bangunan yang mengandung Pb. Toksisitas Pb menghambat sebahagian besar enzim yang terlibat dalam biosintesis heme dan merusak sel hatiTujuan studi ini adalah untuk melihat pengaruh timbal (Pb) terhadap aktivitas enzim katalase hati tikus putih jantan. Desain penelitian ini adalah eksperimental menggunakan 25 ekor tikus putih jantan yang dibagi menjadi lima kelompok, yaitu kelompok kontrol, dan kelompok perlakuan dengan pemberian Pb asetat dengan dosis konsentrasi 5 mg, 10 mg , 20 mg, dan 40 mg/kg BB selama 26 hari.Hasil penelitian menunjukkan terjadinya penurunan rerata aktivitas katalase hati dan serum secara bermakna (p < 0,05), setelah pemberian Pb asetat selama 26 hari. Penurunan aktivitas katalase hati dan serum tikus secara bermakna (p < 0,05) terjadi antara kelompok kontrol dibandingkan dengan kelompok tikus yang diberi dosis 40 mg/kg BB.Kesimpulan yang dapat diambil dari studi ini ialah bahwa pemberian Pb asetat dapat menurunkan aktivitas katalase hati dan juga mempengaruhi aktivitas katalase serum tikus.Kata kunci : Pb asetat, katalaseAbstractLead (Pb) can be found in various environmental media such as air, water, dust and dirt. Pb in the form of compounds derived from burning motor vehicle fuel, industrial emissions and from the use of paints containing Pb buildings. Pb toxicity partly inhibit the enzymes involved in the biosynthesis of heme and damage the liver cells.The purpose of this study was to observe the effect of lead (Pb) on the enzyme activity of rat liver catalase white male. Experimental research design was used 25 white male rats were divided into five groups, namely the control group and theARTIKEL PENELITIAN180group treated with the administration of Pb acetate at a dose concentration of 5 mg, 10 mg, 20 mg, and 40 mg / kg body weight for 26 days.The results showed an average decrease in liver catalase activity, after administration of Pb acetate for 26 days were significantly (p <0.05). Decrease in catalase activity of rat liver and serum were significantly (p <0.05) occurred between the control group compared with the group of mice given a dose of 40 mg / kg.The conclusion from this study is that the administration of Pb acetate can decrease the liver catalase activity and also affects the activity of serum catalase mice.Key word : Pb Acetate, Catalas