16 research outputs found
Study on transepithelial movement of 3H-androgen in the rat seminiferous and epididymal tubules
微小穿刺法と微小灌流法を用いた。精細管の管内アンドロゲン濃度は間質液中のその濃度が10-2, 000nMに増大するに従い直線的に増大したが, 精巣上体頭部における管内アンドロゲン濃度は間質液中の濃度が増大するに従い双曲線的に増大し, 精細管におけるそれよりもはるかに高い値を示した。灌流液中に0.1nMのジニトロフェノール, 0.1mMのKCN, 100μg/mlのサイクロヘキサミドを加えたとき, 管内アンドロゲンの移行は組織ATP濃度とともに有意に減少した。アンドロゲン結合蛋白を含まない人工的精上体頭部の管内液で管内を灌流したとき濃度勾配に抗したアンドロゲン移行は完全に抑制されたThe mechanisms involved in the maintenance of the endocrinological microenvironment of the seminiferous and epididymal tubules were examined in a series of experiments utilizing in vivo microperifusion, microperfusion, and micropuncture technique. The intraluminal 3H-androgen concentration in the seminiferous tubules increased linearly as the interstitial 3H-androgen concentrations increased from 10 nM to 2, 000 nM, but in the caput epididymidal tubules, the intraluminal 3H-androgen concentration increased hyperbolically across the same range of peritubular 3H-androgen concentration. Intraluminal 3H-androgen concentrations in the caput epididymidis did not rise above approximately 340 nM even when the peritubular 3H-androgen concentration exceeded 2, 000 nM. Perifusion of caput tubules with 0.1 mM dinitrophenol or potassium cyanide or 100 micrograms/ml cyclohexamide significantly reduced the proluminal 3H-androgen movement, but tubules perifused with control medium did not support antigrade 3H-androgen movement in the absence of native lumen fluids which contain androgen-binding protein. Energy-requiring protein synthesis is necessary for antigrade 3H-androgen movement in the caput epididymidis, but the mechanism for the interaction of intracellular protein(s) and 3H-androgen movement remains undetermined
Mbechsteinii_Epigenetics
R script used for the data analysis (linear regression, multiple linear regression, LOOCV)
Additional file 2: of Genome-wide methylomic analysis in individuals with HNF1B intragenic mutation and 17q12 microdeletion
Figure S1. This figure illustrates the extent of the 17q12 deletion in each patient as estimated by the CNV calling algorithm within the CHAMP package. (PDF 15 kb
Additional file 5: of Genome-wide methylomic analysis in individuals with HNF1B intragenic mutation and 17q12 microdeletion
Figure S2. This Figure shows four significant differentially methylated regions (DMRs) identified between controls and 17q12 deletion carriers. A) SYNRG (corrected P = 1.32E-17), B) AATF (corrected P = 1.64E-11). C) LHX1 (corrected P = 3.37E-18), D) SMIM24 (corrected P = 1.01E-07). (PDF 223 kb
Additional file 1: of Genome-wide methylomic analysis in individuals with HNF1B intragenic mutation and 17q12 microdeletion
Table S1. SLC1A3 bisulfite pyrosequencing assay conditions. (XLSX 11 kb
Volcano plots representing group comparisons for all genes included in the analysis.
<p>x-axes represent log2 fold-changes and y-axes represent the –log10(p-values) associated with the t-statistic. Vertical dotted lines are positioned at a log2 fold-change of 0.5 or −0.5 and horizontal dotted lines are positioned at the equivalent of p = 0.01. In red are those genes that are differentially expressed at p<0.01 and log2 fold-change>0.5 or <−0.5. A) Nic vs. Con B) Nic vs. Con-Pf and C) Con vs. Con-Pf.</p
The top 5 canonical pathways identified for genes differentially expressed due to food-restriction.
<p>The top 5 canonical pathways identified for genes differentially expressed due to food-restriction.</p
qRT-PCR results for differentially expressed genes in microarray analysis.
<p>qRT-PCR results for differentially expressed genes in microarray analysis.</p
IPA identified a functional network of genes that included 7 genes differentially expressed due to food-restriction.
<p>In <b>bold</b> are the genes that were represented from our list of differentially expressed genes.</p
Gene set enrichment analysis (GSEA) of the GO pathway “RESPONSE_TO_STRESS” in the Con vs. Con-Pf comparison.
<p>The input gene list was all genes in the microarray analysis ranked by –log10(p-value)×log2(fold change). The enrichment score profile displays an enrichment of pathway hits at the top of the list, suggesting multiple top-ranked genes involved in the “Response to stress” pathway.</p