Endoscopy-assisted removal through combined lower and middle meatotomies of an ectopic upper third molar in the sinus associated with a dentigerous cyst

The aim of this case report is to present an original conservative technique for the transnasal endoscopy-assisted extraction of an ectopic upper third molar associated with a dentigerous cyst occupying the whole maxillary sinus by means of combined lower and middle meatotomies. The proposed technique is a viable, minimally-invasive alternative to the Caldwell–Luc operation (with or without the repositioning of a bone lid), and also to endoscopic middle meatal antrostomy in cases where this would be unable to ensure adequate access because of the position and size of the ectopic tooth and associated cyst

T Dependence of the Mechanical Properties on the Microstructural Parameters of WC-Co

Abstract The effect of binder content and WC grain size on the mechanical properties is widely investigated in literature. An increase in binder amount and WC grain size leads to a decrease in hardness and an increase in fracture toughness. Actually, these correlations are related to the influence of binder content and WC grain size through the contiguity and mean binder free path, which are the microstructural parameters that affect the mechanical properties. The aim of this study is to verify the dependence of the two microstructural parameters that govern the WCCo mechanical behaviour, namely the contiguity and mean binder free path, on the mechanical properties of an extended range of WC-Co samples, which differ in terms of Co content and tungsten carbide grain size

Composição proximal em filés, rendimento de carcaça e de cortes de jundiá (rhamdia quelen) alimentados com rações contendo diferentes óleos vegetais.

Este trabalho teve por objetivos avaliar a influência de óleos vegetais no desenvolvimento corporal de jundiá (Rhamdia quelen, Heptapteridae) e sua relação com o rendimento de carcaça, partes comestíveis e composição química do filé. Testaram-se seis rações com 32% de proteína bruta, sendo avaliados óleos de arroz, canola ou soja com 5 e 10% de inclusão durante 90 dias. Foram utilizados 180 peixes (peso inicial=71±0,81g) distribuídos ao acaso em 18 caixas de 280L (10 peixes/caixa) em um sistema de recirculação de água, com temperatura controlada (25,9±0,9ºC). Não houve diferenças para os parâmetros produtivos entre os tratamentos testados ao final do experimento (P<0,05). Os peixes alimentados com maiores níveis de óleo nas dietas depositaram maior porcentagem de gordura no filé. Conclui-se que os óleos de canola, arroz e soja utilizados como alternativas em dietas na recria de jundiá proporcionam bom rendimento de carcaça e de partes comestíveis. E, as rações contendo óleo de canola possibilitam menor deposição de gordura no filé de juvenis de jundiá em nível de 5%

Transcriptional activation of pericentromeric satellite repeats and disruption of centromeric clustering upon proteasome inhibition

Heterochromatinisation of pericentromeres, which in mice consist of arrays of major satellite repeats, are important for centromere formation and maintenance of genome stability. The dysregulation of this process has been linked to genomic stress and various cancers. Here we show in mice that the proteasome binds to major satellite repeats and proteasome inhibition by MG132 results in their transcriptional de-repression; this de-repression is independent of cell-cycle perturbation. The transcriptional activation of major satellite repeats upon proteasome inhibition is accompanied by delocalisation of heterochromatin protein 1 alpha (HP1α) from chromocentres, without detectable change in the levels of histone H3K9me3, H3K4me3, H3K36me3 and H3 acetylation on the major satellite repeats. Moreover, inhibition of the proteasome was found to increase the number of chromocentres per cell, reflecting destabilisation of the chromocentre structures. Our findings suggest that the proteasome plays a role in maintaining heterochromatin integrity of pericentromeres

The antitumor drug, 1,3-bis(2-chloroethyl)-1-nitroso-urea, inactivates human nicotinamide mononucleotide adenylyltransferase.

Nicotinamide mononucleotide (NMN) adenylyltransferase (EC 2.7.7.1) from human placenta is rapidly inactivated by 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU). A similar inactivation is observed with other C- and N-nitroso compounds. The inactivation by BCNU is dependent on incubation time, temperature and BCNU concentration. Protective reagents for -SH groups, dithiothreitol and beta-mercaptoethanol, and the substrate NMN are very effective in protecting NMN adenylyltransferase from BCNU inactivation and in preserving its catalytic properties, while ATP is less efficient. Incubation of BCNU-inactivated and dialysed NMN adenylyltransferase with dithiothreitol results in a partial recovery of the enzymatic activity

Densidade de estocagem no crescimento, composição e perfil lipídico corporal do jundiá.

O objetivo do trabalho foi avaliar o crescimento, a composição e o perfil lipídico corporal de jundiás criados em diferentes densidades de estocagem (DE: 4,2; 6,4; 8,6 e 10,8kg m-3) em sistema com recirculação de água. Durante 120 dias, 420 peixes (peso inicial=182,95±2,96g) foram alimentados uma vez ao dia, até a saciedade aparente, com dieta peletizada formulada. Observou-se que, quanto maior a DE utilizada, menor é o peso individual dos peixes, entretanto a biomassa por tanque aumentou (P<0,05). Melhor conversão alimentar (1,69) e maior quantidade de gordura intraperitoneal (3,92) foram observadas nos peixes submetidos à densidade 4,2kg m-3. A proteína corporal foi afetada pelas DEs. Ocorreu diminuição dos ácidos graxos saturados e aumento dos insaturados nos peixes de acordo com o aumento das DEs. Conclui-se que a densidade de estocagem influência no peso final e na produtividade por volume do jundiá

Nicotinamide N-methyltransferase catalyses the N-methylation of the endogenous ß-carboline norharman: evidence for a novel detoxification pathway

Nicotinamide N-methyltransferase (NNMT) is responsible for the N-methylation of nicotinamide to 1-methylnicotinamide. Our recent studies have demonstrated that NNMT regulates cellular processes fundamental to the correct functioning and survival of the cell. It has been proposed that NNMT may possess β-carboline (BC) N-methyltransferase activity, endogenously and exogenously produced pyridine-containing compounds which, when N-methylated, are potent inhibitors of Complex I and have been proposed to have a role in the pathogenesis of Parkinson's disease. We have investigated the ability of recombinant NNMT to N-methylate norharman (NH) to 2-N-methylnorharman (MeNH). In addition, we have investigated the toxicity of the BC NH, its precursor 1,2,3,4-tetrahydronorharman (THNH) and its N-methylated metabolite MeNH, using our in vitro SH-SY5Y NNMT expression model. Recombinant NNMT demonstrated NH 2N-methyltransferase activity, with a Km of 90 ± 20 µM, a kcat of 3 × 10(-4) ± 2 × 10(-5) s(-1) and a specificity constant (kcat/Km) of 3 ± 1 s(-1) M(-1) THNH was the least toxic of all three compounds investigated, whereas NH demonstrated the greatest, with no difference observed in terms of cell viability and cell death between NNMT-expressing and non-expressing cells. In NNMT-expressing cells, MeNH increased cell viability and cellular ATP concentration in a dose-dependent manner after 72 and 120 h incubation, an effect that was not observed after 24 h incubation or in non-NNNT-expressing cells at any time point. Taken together, these results suggest that NNMT may be a detoxification pathway for BCs such as NH

The hepatoprotective effect of jaboticaba peel powder in a rat model of type 2 Diabetes Mellitus involves the modulation of thiol/disulfide redox state through the upregulation of glutathione synthesis

Jaboticaba peel powder (JPP) is rich in bioactive compounds, mainly soluble and insoluble polyphenols with great antioxidant properties. ,e aim of this study is to evaluate the effects of JPP supplementation on the oxidative stress and hepatic damage in a rat model of type 2 diabetes mellitus (T2DM). Diabetic rats received vehicle or JPP at 2.7 (JPP-I), 5.4 (JPP-II), or 10.8 (JPP-III) g/L in drinking water during 8 weeks. JPP-III attenuated hyperglycaemia and dyslipidemia increased by 86% the liver content of nonprotein thiol groups and by 90% the GSH/GSSG ratio by activating glutathione synthesis. Accordingly, JPP supplementation prevented the loss of activity of the sulfhydryl-dependent enzyme δ-aminolaevulinic acid dehydratase and attenuated hepatic injury assessed by the reduction of serum aspartate aminotransferase activity and liver hypertrophy. Our results support that JPP supplementation to T2DM rats decreases hepatic damage most likely by increasing glutathione synthesis and modulating the thiol/disulfide redox balance