95 research outputs found
First Report of Anthracnose Disease Caused by Colletotrichum acutatum on Lupinus albus in Italy.
Pulmonary Hypertension in Patients with Treated Pulmonary Tuberculosis: Analysis of 14 Consecutive Cases
Background Pulmonary tuberculosis (PTB) is an increasing global health problem that continues to cause significant morbidity and mortality. The impact of PTB has been measured in terms morbidity and mortality and little attention has been paid to continuing respiratory disability in those who were cured. Pulmonary hypertension (PHT) is a serious respiratory disability that results from structural lung damage and chronic hypoxia. This study was conducted to investigate the presence of PHT in a cohort of treated PTB patients who presented with shortness of breath. Methods This is a cross-sectional study that included 14 consecutive patients who were cured of PTB and presented with shortness of breath. Demographic and clinical data were recorded for all patients. PHT was diagnosed using Doppler echocardiography. Results Fourteen patients who were treated for PTB and were found to have PHT were studied. All patients were sputum smear negative at the time of the study. The mean age (SD) was 43.1 (13.6) and half of the patients were males. The mean number of years since PTB was diagnosed (SD) was 9.4 (10.9). All patients had abnormal chest x-rays. The commonest radiological abnormality was fibrocavitation which occurred in 50% of patients. Estimated pulmonary artery systolic pressure (PASP) of 51 to 80 mm/Hg was found in 9 patients (64.3%) whereas PASP of 40 to 50 mm/Hg was found in 4 patients (28.6%) and one patient had PASP more than 80 mm/Hg. Conclusions Different grades of PHT occurred in this cohort of treated PTB patients on average about 9 years after cure. The findings of this study support implementation of strategies for early detection and prevention of PTB. For those who were cured from PTB, longer periods of disability should be implemented in assessment of disease burden
Study of Bio-Pharmaceutical and Antimicrobial Properties of Pomegranate (Punica granatum L.) Leathery Exocarp Extract
Pomegranate (Punica granatum L.) fruits are important sources of vitamins and minerals
and widely used in the dietary supplement industry. An aqueous extract of its leathery exocarp
(LEP) was obtained by a solid-phase micro-extraction method. The antifungal activity was examined
against the phytopathogenic fungi, Fusarium oxysporum, Phytophthora cinnamomi, Penicillium digitatum
and Botrytis cinerea, and the antibacterial activity was evaluated against Escherichia coli, Xanthomonas
campestris, Bacillus megaterium and Clavibacter michiganensis. The antimicrobial assays showed, in
some cases, a promising antimicrobial effect compared to the synthetic drugs. The possible antiacetylcholinesterase
and antioxidant activities of the LEP extract were investigated by the Ellman’s
assay and 2,2-diphenyl-1-picrylhydrazyl (DPPH) test, respectively, and their results showed that the
LEP extract has an effective anti-acetylcholinesterase inhibitory effect and an antioxidant activity.
Thus, the LEP extract could be valid as a candidate for further studies on the use of pomegranate
in neurodegenerative diseases as a food preservative and a suitable substitute to control several
phytopathogens
Morpho-anatomical and microbiological analysis of kiwifruit roots with KVDS symptoms
Italy, one of the largest producers of kiwifruit in the world, has lost 10% of its production in recent years because of the spread of the kiwifruit vine decline syndrome (KVDS). Although the aetiology of KVDS has not been characterized, root rot symptoms are often associated with water stagnation and root asphyxia. To investigate causal factors and potential solutions to counter this syndrome, an experimental trial was undertaken in a kiwifruit orchard affected by KVDS in Latina (central Italy) in 2020. Root samples from healthy plants were collected and compared with samples taken from plants affected by KVDS. Macroscopically, the roots affected by KVDS were rotting, showing a loss of rhizodermis and cortical parenchyma. Microscopic analysis revealed damage to the root system with tissue breakdown and decomposition, flaking of the rhizodermis, cortical area with a clear loss of cell turgor, initial decay of the stele and evident detachment of the cortex from the central conducting tissues. Light microscopy, morphological and molecular analyses were carried out on the rhizodermis of roots showing decay and death symptoms. Total DNA extracted from the pure fungal colonies was amplified by PCR with ITS primers, amplicons directly sequenced, and the obtained nucleotide sequences were compared with those present in the GenBank database (NCBI) through BLAST analysis. Genomic analysis allowed the identification of three abundant fungi namely Ilyonectria vredenhoekensis, Fusarium oxysporum and Paraphaeosphaeria michotii. Further investigation is required to determine the role of these fungi in KVDS, whether they are species favoured by water stagnation and root asphyxia; their abundance and presence in other regions, orchards, and kiwifruit species; if they compromise roots functionality individually or conjunction with other microbial pathogens or abiotic factors; and if they contribute to plant death associated with KVDS
Use of plant growth promoting bacteria (PGDB) for promoting tomato growth and its evaluation as biological control agent.
The plant growth promoting effect of some bacteria usable to control plant diseases has a potential for resolving technical problems of plant production. The effects of Burkholderia gladioli pv. agaricicola ICMP11096 on development of tomato seedlings development and control of their infection by Fusarium oxysporum and Sclerotinia sclerotiorum were evaluated in the present study. Bacterized plants showed a higher shoot and root weight and a more extended root system in terms of total length and root density than non-bacterized plants. The bacter-izing treatments gave rise to significant reduction of disease symptoms such as leaf wilting induced by F. oxysporum infection and/or root ne-crosis and leaf yellowing by S. sclerotiorum. The potential use of Burkholderia gladioli pv. agaricicola ICMP11096 in biocontrol of fungal patho-gens and to enhance seedling growth parameters is underlined as a tool to achieve a high commercial quality of nursery tomato plants
Antimicrobial and Phytotoxic activity of essential oil of Origanum vulgare growing in Cilento
Biochemical Characterization and Antimicrobial Activity Against Some Human or Phyto-Pathogens of New Diazonium Heterocyclic Metal Complexes
String of vanadium (IV), zirconium (IV), palladium (II), platinum (IV) and uranium (VI) chelates of 2-cyano-2-[(2-
nitrophenyl)hydrazono]thioacetamide (Cnphta) were prepared and characterized by physicochemical, spectroscopic and thermal analyses. The formulae of the isolated solid complexes were assigned as [VO-
(Cnphta)2(H2O)]SO4 ·5H2O (1), [ZrO(Cnphta)2(H2O)]Cl2 ·4H2O (2), [Pd(Cnphta)2]Cl2 (3), [Pt(Cnphta)2Cl2]Cl2 (4) and
[UO2(Cnphta)2](NO3)2 ·5H2O (5). The infrared assignments clearly showed that Cnphta ligand coordinated as a
bidentate feature through the hydrazono nitrogen and the thioacetamide nitrogen for V(IV), Zr(IV) and U(VI) but
displayed different behavior for Pd(II) and Pt(IV). Results of the molar conductivities measurements showed that
the metal complexes were electrolytes in contrast with Cnphta ligand. The interpretation, mathematical analysis
and evaluation of kinetic parameters were also carried out. In addition, the studied ligand and its new chelates
were tested for their antimicrobial activity against some human or phytopathogenic microorganisms. The new
metal complexes explicated promising antibacterial activity against all tested bacteria especially Staphylococcus
aureus and Bacillus subtilis. Regarding the antifungal activity, all metal complexes were able to inhibit the
mycelium growth of both tested pathogenic fungi. In particular Zr(IV) and Pt(IV) complexes showed the highest
significant fungicidal effect against A. fumigatus similar to positive contro
Genome-wide analysis identified candidate variants and genes associated with heat stress adaptation in Egyptian sheep breeds
Heat stress caused by climatic changes is one of the most significant stresses on livestock in hot and dry areas. It has particularly adverse effects on the ability of the breed to maintain homeothermy. Developing countries are advised to protect and prepare their animal resources in the face of potential threats such as climate change. The current study was conducted in Egypt’s three hot and dry agro-ecological zones. Three local sheep breeds (Saidi, Wahati, and Barki) were studied with a total of 206 ewes. The animals were exercised under natural heat stress. The heat tolerance index of the animals was calculated to identify animals with high and low heat tolerance based on their response to meteorological and physiological parameters. Genomic variation in these breeds was assessed using 64,756 single nucleotide polymorphic markers (SNPs). From the perspective of comparative adaptability to harsh conditions, our objective was to investigate the genomic structure that might control the adaptability of local sheep breeds to environmental stress under hot and dry conditions. In addition, indices of population structure and diversity of local breeds were examined. Measures of genetic diversity showed a significant influence of breed and location on populations. The standardized index of association (rbarD) ranged from 0.0012 (Dakhla) to 0.026 (Assuit), while for the breed, they ranged from 0.004 (Wahati) to 0.0103 (Saidi). The index of association analysis (Ia) ranged from 1.42 (Dakhla) to 35.88 (Assuit) by location and from 6.58 (Wahati) to 15.36 (Saidi) by breed. The most significant SNPs associated with heat tolerance were found in the MYO5A, PRKG1, GSTCD, and RTN1 genes (p ≤ 0.0001). MYO5A produces a protein widely distributed in the melanin-producing neural crest of the skin. Genetic association between genetic and phenotypic variations showed that OAR1_18300122.1, located in ST3GAL3, had the greatest positive effect on heat tolerance. Genome-wide association analysis identified SNPs associated with heat tolerance in the PLCB1, STEAP3, KSR2, UNC13C, PEBP4, and GPAT2 genes
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