Assessment of natural radionuclides and heavy metal concentrations in marine sediments in view of tourism activities in Hurghada city, northern Red Sea, Egypt

The specific activity of 40K, 232Th and 226Ra in marine sediment samples collected from National Institute of Oceanography and Fisheries (NIOF) and Safier Hotel area in Hurghada city (the most important regions in Egypt), were measured by gamma ray spectrometry using NaI(Tl) detector. The values of specific activity varied from 7 ± 1 Bq kg-1 to 53 ± 4 Bq kg-1, 6 ± 1 Bq kg-1 to 32 ± 6 Bq kg-1, and from 167 ± 11 Bq kg-1 to 1120 ± 63 Bq kg-1 for 226Ra, 232Th and 40K, respectively. The heavy metals have been measured and analysed by atomic absorption spectrometer. The major range values of heavy metals concentrations in marine sediment samples were: Cu (10.5-78.0 μg g-1), Zn (21-150 μg g-1), Pb (30-53 μg g-1), Cd (2.50-4.00 μg g-1), Fe (5100-13150 μg g-1), Mn (118-298 μg g-1), Ni (17-36 μg g-1) and Co (16-18 μg g-1). The total organic matter (TOC) and carbonates (CaCo3) distribution have been measured at some locations. Also, the frequency distribution and the value of (232Th/226Ra), (232Th/40K) and (226Ra/40K) ratio for all measured samples were determined. Additionally, evaluations have been made of the radiological hazards and the results are diagrammed by Surfer program in maps. © Penerbit Universiti Sains Malaysia, 2019

The influence of heavy elements on the ionizing radiation shielding efficiency and elastic properties of some tellurite glasses: Theoretical investigation

The impact of adding PbO and WO3 on the mechanical properties and radiation shielding efficiency of 4 different glass samples labeled as TWP1, TWP2, TWP3, and TWP4 (samples codes) was studied via the Makishima and Mackenzie model (MMD), Rocherulle model (RD), XCOM database, FLUKA code, and Phys-X/PSD software. According to MMD, Young's (Y), bulk (K), shear (G) modulus values increase from 59.13 to 62.80 GPa, from 37.76 to 43.86 GPa, from 25.48 to 26.55 GPa, and from 71.73 to 79.26 GPa for TWP1 and TWP4 glasses, respectively. 277.833, 358.768, 465.048 and 570.786 cm−1 values are the highest linear attenuation coefficient (μ) values for TWP1, TWP2, TWP3 and TWP4 glasses at 0.015 MeV. The results refer that the TWP4 glass sample has the highest radiation shielding and mechanical properties. The results indicated that the addition of lead and tungsten to the investigated samples improves their elastic and radiation shielding properties. Thus, the TWP4 sample is the best compared to the other glass samples. © 2020 The Author

The Influence of CoO/P2O5 Substitutions on the Structural, Mechanical, and Radiation Shielding of Boro-Phosphate Glasses

A new glass system (50−x)P2 O5 –20B2 O3 –5Al2 O3 –25Na2 O–xCoO was manufactured using a standard melt quenching procedure, where 1≤ x ≤ 12 mol%. The characteristics of boro-phosphate-glasses containing CoO have been studied. The effect of CoO on the radiation-protective properties of glasses was established. The density of the prepared glasses as a function of CoO increased. XRD was used to check the vitreous structure of samples. Fourier-transform infrared (FTIR) spectroscopy was used to study the structure of each sample. FTIR demonstrated that connections grew as CoO/P2 O5 levels increased, and the FTIR spectra shifted to higher wavenumbers. The increment of CoO converts non-bridging oxygens associated with phosphate structural units into bridging oxygens. This process increases the concentration of BO4 structural units and creates new, strong and stable bonds B–O–P, i.e., there is polymerization of the boro-phosphate glass network. With an increase in the ratio of CoO/P2 O5 in the produced samples, ultrasonic velocities and elastic moduli were observed to increase. The coefficients of linear and mass attenuation, the transmittance of photons in relation to the photon energy, the efficiency of radiation protection in relation to the photon energy, and the thickness of the absorber were modeled using these two methods (experimental and theoretical). From the obtained values, it can be concluded that the 12Co sample containing 12 mol% will play the most influential role in radiation protection. An increase in the content of cobalt-I oxide led to a significant increase in the linear and mass attenuation coefficient values, which directly contributes to the development of the radiation-protective properties of glass. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.Funding: This work funding by Deanship of Scientific at Jouf University through research grant no (DSR2020-02-504)

Discovery of novel natural products as dual MNK/PIM inhibitors for acute myeloid leukemia treatment: Pharmacophore modeling, molecular docking, and molecular dynamics studies

MNK-2 and PIM-2 kinases play an indispensable role in cell proliferation signaling pathways linked to tyrosine kinase inhibitors resistance. In this study, pharmacophore modeling studies have been conducted on the co-crystalized ligands of MNK-2 and PIM-2 enzyme crystal structures to determine the essential features required for the identification of potential dual inhibitors. The obtained pharmacophore features were then screened against a library of 270,540 natural products from the ZINC database. The matched natural molecules were docked into the binding sites of MNK-2 and PIM-2 enzymes. The compounds with high docking scores with the two enzymes were further subjected to MM-GBSA calculations and ADME prediction. This led to the identification of compound 1 (ZINC000085569211), compound 2 (ZINC000085569178), and compound 3 (ZINC000085569190), with better docking scores compared to the reference co-crystallized ligands of MNK-2 and PIM-2. Moreover, compounds 1‒3 displayed better MM-GBSA binding free energies compared to the reference ligands. Finally, molecular dynamics (MD) study was used to assess the interaction stability of the compounds with MNK-2. To this end, compounds 1 and 3 bound strongly to the target during the whole period of MD simulation. The findings of the current study may further help the researchers in the discovery of novel molecules against MNK-2 and PIM-2

Congenital deficiency reveals critical role of ISG15 in skin homeostasis

Ulcerating skin lesions are manifestations of human ISG15 deficiency, a type I interferonopathy. However, chronic inflammation may not be their exclusive cause. We describe two siblings with recurrent skin ulcers that healed with scar formation upon corticosteroid treatment. Both had a homozygous nonsense mutation in the ISG15 gene, leading to unstable ISG15 protein lacking the functional domain. We characterized ISG15(-/-) dermal fibroblasts, HaCaT keratinocytes, and human induced pluripotent stem cell-derived vascular endothelial cells. ISG15-deficient cells exhibited the expected hyperinflammatory phenotype, but also dysregulated expression of molecules critical for connective tissue and epidermis integrity, including reduced collagens and adhesion molecules, but increased matrix metalloproteinases. ISG15(-/-) fibroblasts exhibited elevated ROS levels and reduced ROS scavenger expression. As opposed to hyperinflammation, defective collagen and integrin synthesis was not rescued by conjugation-deficient ISG15. Cell migration was retarded in ISG15(-/-) fibroblasts and HaCaT keratinocytes, but normalized under ruxolitinib treatment. Desmosome density was reduced in an ISG15(-/-) 3D epidermis model. Additionally, there were loose architecture and reduced collagen and desmoglein expression, which could be reversed by treatment with ruxolitinib/doxycycline/TGF-beta 1. These results reveal critical roles of ISG15 in maintaining cell migration and epidermis and connective tissue homeostasis, whereby the latter likely requires its conjugation to yet unidentified targets