10,359 research outputs found
Determination of Polarized PDFs from a QCD Analysis of Inclusive and Semi-inclusive Deep Inelastic Scattering Data
A new combined next to leading order QCD analysis of the polarized inclusive
and semi-inclusive deep inelastic lepton-hadron scattering (DIS) data is
presented. In contrast to previous combined analyses, the terms
(kinematic - target mass corrections, and dynamic - higher twist corrections)
in the expression for the nucleon spin structure function are taken into
account. The new COMPASS data are included in the analysis. The impact of the
semi-inclusive data on the polarized parton densities (PDFs) and on the higher
twist corrections is discussed. The new results for the PDFs are compared to
our (Leader, Sidorov, Stamenov) LSS'06 PDFs, obtained from the fit to the
inclusive DIS data alone, and to those obtained from the de Florian, Sassot,
Stratmann, and Vogelsang global analysis.Comment: 11 pages, 8 figures, a version to be published in Phys. Rev.
Restriction Fragment Length Polymorphism Linkage Map for Arabidopsis thaliana
We have constructed a restriction fragment length polymorphism linkage map for the nuclear genome of the flowering plant Arabidopsis thaliana. The map, containing 90 randomly distributed molecular markers, is physically very dense; >50% of the genome is within 1.9 centimorgans, or approx 270 kilobase pairs, of the mapped DNA fragments. The map was based on the meiotic segregation of markers in two different crosses. The restriction fragment length polymorphism linkage groups were integrated with the five classically mapped linkage groups by virtue of mapped mutations included in these crosses. Markers consist of both cloned Arabidopsis genes and random low-copy-number genomic DNA clones that are able to detect polymorphisms with the restriction enzymes EcoRI, Bgl II, and/or Xba I. These cloned markers can serve as starting points for chromosome walking, allowing for the isolation of Arabidopsis genes of known map location. The restriction fragment length polymorphism map also can associate clones of unknown gene function with mutant phenotypes, and vice versa
MscS-like Proteins Control Plastid Size and Shape in Arabidopsis thaliana
Background
Mechanosensitive (MS) ion channels provide a mechanism for the perception of mechanical stimuli such as sound, touch, and osmotic pressure. The bacterial MS ion channel MscS opens in response to increased membrane tension and serves to protect against cellular lysis during osmotic downshock. MscS-like proteins are found widely in bacterial and archaeal species and have also been identified in fission yeast and plants. None of the eukaryotic members of the family have yet been characterized.
Results
Here, we characterize two MscS-like (MSL) proteins from Arabidopsis thaliana, MSL2 and MSL3. MSL3 can rescue the osmotic-shock sensitivity of a bacterial mutant lacking MS-ion-channel activity, suggesting that it functions as a mechanosensitive ion channel. Arabidopsis plants harboring insertional mutations in both MSL3 and MSL2 show abnormalities in the size and shape of plastids, which are plant-specific endosymbiotic organelles responsible for photosynthesis, gravity perception, and numerous metabolic reactions. MSL2-GFP and MSL3-GFP are localized to discrete foci on the plastid envelope and colocalize with the plastid division protein AtMinE.
Conclusions
Our data support a model wherein MSL2 and MSL3 control plastid size, shape, and perhaps division during normal plant development by altering ion flux in response to changes in membrane tension. We propose that MscS family members have evolved new roles in plants since the endosymbiotic event that gave rise to plastids
Group projector generalization of dirac-heisenberg model
The general form of the operators commuting with the ground representation
(appearing in many physical problems within single particle approximation) of
the group is found. With help of the modified group projector technique, this
result is applied to the system of identical particles with spin independent
interaction, to derive the Dirac-Heisenberg hamiltonian and its effective space
for arbitrary orbital occupation numbers and arbitrary spin. This gives
transparent insight into the physical contents of this hamiltonian, showing
that formal generalizations with spin greater than 1/2 involve nontrivial
additional physical assumptions.Comment: 10 page
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Effects of therapeutic hypothermia on white matter injury from murine neonatal hypoxia-ischemia.
BackgroundTherapeutic hypothermia (TH) is the standard of care for neonates with hypoxic-ischemic encephalopathy, but it is not fully protective in the clinical setting. Hypoxia-ischemia (HI) may cause white matter injury (WMI), leading to neurological and cognitive dysfunction.MethodsP9 mice were subjected to HI as previously described. Pups underwent 3.5 h of systemic hypothermia or normothermia. Cresyl violet and Perl's iron staining for histopathological scoring of brain sections was completed blindly on all brains. Immunocytochemical (ICC) staining for myelin basic protein (MBP), microglia (Iba1), and astrocytes (glia fibrillary acidic protein (GFAP)) was performed on adjacent sections. Volumetric measurements of MBP coverage were used for quantitative analysis of white matter.ResultsTH provided neuroprotection by injury scoring for the entire group (n=44; P<0.0002). ICC analysis of a subset of brains showed that the lateral caudate was protected from WMI (P<0.05). Analysis revealed decreased GFAP and Iba1 staining in hippocampal regions, mostly CA2/CA3. GFAP and Iba1 directly correlated with injury scores of normothermic brains.ConclusionTH reduced injury, and qualitative data suggest that hippocampus and lateral caudate are protected from HI. Mildly injured brains may better show the benefits of TH. Overall, these data indicate regional differences in WMI susceptibility and inflammation in a P9 murine HI model
Autonomous control of underground mining vehicles using reactive navigation
Describes how many of the navigation techniques developed by the robotics research community over the last decade may be applied to a class of underground mining vehicles (LHDs and haul trucks). We review the current state-of-the-art in this area and conclude that there are essentially two basic methods of navigation applicable. We describe an implementation of a reactive navigation system on a 30 tonne LHD which has achieved full-speed operation at a production mine
Status of Neutrino Masses and Mixing and Future Perspectives
Status of the problem of neutrino masses, mixing and oscillations is
discussed. Future perspectives are briefly considered.Comment: Report at the conference IRGAC 2006, Barcelona July 11-15 200
Segmentation, Reconstruction, and Analysis of Blood Thrombus Formation in 3D 2-Photon Microscopy Images
We study the problem of segmenting, reconstructing, and analyzing the structure growth of thrombi (clots) in blood vessels in vivo based on 2-photon microscopic image data. First, we develop an algorithm for segmenting clots in 3D microscopic images based on density-based clustering and methods for dealing with imaging artifacts. Next, we apply the union-of-balls (or alpha-shape) algorithm to reconstruct the boundary of clots in 3D. Finally, we perform experimental studies and analysis on the reconstructed clots and obtain quantitative data of thrombus growth and structures. We conduct experiments on laser-induced injuries in vessels of two types of mice (the wild type and the type with low levels of coagulation factor VII) and analyze and compare the developing clot structures based on their reconstructed clots from image data. The results we obtain are of biomedical significance. Our quantitative analysis of the clot composition leads to better understanding of the thrombus development, and is valuable to the modeling and verification of computational simulation of thrombogenesis
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