Influence of feed delivery frequency on behavioural activity of dairy cows in freestall barns

Research on feeding management in more competitive free-stall settings indicates that frequency of delivery of fresh feed stimulates feed bunk attendance and can affect other aspects of cows’ time budgets apart from feeding such as time spent standing vs. lying down. The objective of this study was to examine how the frequency of feed delivery affects the behavior in two farms, one with a conventional and one with automatic milking system (AMS). The feeding frequency was varied from two to three times per day in the conventional dairy farm; one to two times per day in the AMS farm. The experiment was carried out in two different seasons. All behaviours of the cows were monitored in continuous by video recording. As expected, behavioral indices have been significantly affected by environmental conditions both in conventional farm and AMS farm. The variation in the frequency of feed delivery seems to affect the cow behavioural activity only in a limited way and modify only slightly the daily averages of the time spent in different activities mainly increasing the time cows spend standing (+4- 5%)

HIV-2 infection in a migrant from Gambia: the history of the disease combined with phylogenetic analysis revealed the real source of infection

Human immunodeficiency virus type 2 (HIV-2) infection prevalence is increasing in some European countries. The increasing migratory flow from countries where HIV-2 is endemic has facilitated the spread of the virus into Europe and other regions. We describe a case of HIV-2 infection in a migrant individual in the Asylum Seeker Centre (ASC) in Italy. The patient's virus was sequenced, and found to be a typical HIV-2 genotype A virus. Bayesian evolutionary analysis revealed that the HIV-2 sequence from migrant dated back to 1986 in a subcluster including sequences from Guinea Bissau. This was coherent with the migrant history who lived in Guinea Bissau from his birth until 1998 when he was 13 years old. Monitoring for HIV-2 infection in migrants from western Africa is necessary using adequate molecular tools to improve the diagnosis and understand the real origin of infection

Nutritional value of a rice-hydrolysate formula in infants with cows' milk protein allergy: a randomized pilot study.

This study was designed to assess whether a rice-hydrolysate formula allows normal growth and adequate metabolic balance in infants with cows' milk protein allergy. Infants (seven females, nine males; aged 6–14 months) were randomly assigned to receive a rice-hydrolysate formula ( n = 8) or a soy formula (control group, n = 8). Standardized growth indices ( Z scores) and biochemical parameters were evaluated during a 6-month treatment period. Infants in both groups showed normal growth patterns during the study, and no adverse reactions were seen. Mean plasma biochemical parameters were within the normal ranges, and did not differ between groups. In conclusion, rice-hydrolysate formula may be a nutritionally suitable alternative for infants with cows' milk protein allergy. Larger studies, with satisfactory power, should be undertaken to confirm these findings

Relationships among lying and standing behaviour, body condition score and milk production in primiparous cows

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A Specific Prebiotic Mixture Added to Starting Infant Formula Has Long-Lasting Bifidogenic Effects123

There is some evidence that early colonization of the intestine affects the composition of the intestinal microbiota after weaning. In the present study, the effect of prebiotics administered from the first day of life on fecal counts of bifidobacteria and lactobacilli were studied during and after the administration of the prebiotics. In this double-blind, randomized, placebo-controlled, explorative study, 20 newborns of hepatitis C virus-infected mothers who decided not to breast feed due to their concerns regarding their plasma viral load were randomly assigned to either a formula with 8 g/L of a specific prebiotic mixture (short-chain galacto-oligosaccharides and long-chain fructo-oligosaccharides, ratio 9:1) or a formula containing the same amount of maltodextrin (placebo). Clinical examination including anthropometric measurements, microbiological analysis of fecal samples, and blood leukocyte population analysis were performed at birth and 3, 6, and 12 mo age. At the age of 12 mo, hepatitis B vaccine-specific IgG serum titers (Hepatitis B virus surface antibodies) were also measured. Prebiotic supplementation resulted in more fecal bifidobacteria (P < 0.0001) and lactobacilli (P = 0.0044) compared with the placebo group. These differences between the groups were maintained during the second half of the first year without any prebiotic supplementation. There was no influence of the different diets on anthropometric data or the measured immunological variables. The data from this small explorative study indicate that early colonization of the intestine might have long-lasting effects on the composition of the intestinal microbiota

Post-Operative Infections in Head and Neck Cancer Surgery: Risk Factors for Different Infection Sites

Background: Post-operative infections in head and neck cancer (HNC) surgery represent a major problem and are associated with an important increase in mortality, morbidity, and burden on the healthcare system. The aim of this retrospective observational study was to evaluate post-operative infections in HNC surgery and to analyze risk factors, with a specific focus on different sites of infection. Methods: Clinical data about 488 HNC patients who underwent surgery were recorded. Univariate and multivariate analyses were performed to identify risk factors for post-operative infections. Results: Post-operative infections were observed in 22.7% of cases. Respiratory and surgical site infections were the most common. Multiple site infections were observed in 3.9% of cases. Considering all infection sites, advanced stage, tracheotomy, and higher duration of surgery were risk factors at multivariate analysis. Median hospital stay was significantly longer in patients who had post-operative infection (38 vs. 9 days). Conclusions: Post-operative infections may negatively affect surgical outcomes. A correct identification of risk factors may help the physicians to prevent post-operative infections in HNC surgery

Differential expression of interferon-induced microRNAs in patients with chronic hepatitis C virus infection treated with pegylated interferon alpha

There have been reports of in-vitro interferon (IFN)-mediated antiviral activity against the hepatitis C virus (HCV) through microRNAs (miRNAs). The main aim of this study was to evaluate the expression of several miRNAs (miR-1, miR-30, miR-128, miR-196, miR-296) in peripheral blood mononuclear cells (PBMCs) from healthy individuals after in vitro IFN-treatment and in PBMCs from patients with chronic hepatitis C (CHC) before and 12 hours after the first injection of pegylated IFN alpha. We demonstrated that expression of these miRNAs could be recorded in PBMCs collected from healthy individuals before and after in-vitro IFN alpha treatment. Our analysis revealed that the levels of expression of all miRNAs investigated in patients with CHC were different to those in healthy individuals. When levels of the miRNAs were measured 12 hours after the first IFN injection, increases in expression levels of IFN-induced miRNAs were observed in 25-50% of patients, depending on the type of miRNA examined. No correlations were observed between HCV viral load, alanine aminotransferase status and expression of miRNA. Together these findings suggest that: (i) IFN alpha in-vitro treatment of PBMCs leads to a transcriptional induction of all miRNAs investigated; (ii) miRNAs can be induced differentially by IFN treatment in patients with HCV. Given the importance of miRNAs in defending the host against virus infections, it is possible that IFN-induced miRNAs may represent an important determinant of the clinical outcome of IFN therapy in HCV infection

Real-time automatic integrated monitoring of barn environment and dairy cattle behaviour: Technical implementation and evaluation on three commercial farms

Due to increasing herd sizes and automation on dairy farms there is an important need for automated monitoring of cow production, health, and welfare. Despite much progress in automatic monitoring techniques, there is still a need to integrate data from multiple sources to create a comprehensive overview and accurate diagnosis of a cow’s state. To aid the technological development of data integration, a prototype of an open and customizable automatic system that integrates data from multiple sensors relating to barn environment and cow behaviour was developed. The system integrates data from sensors that measure barn climate (e.g., temperature, humidity, wind speed), air quality (e.g., CO2 concentration), water use and temperature, the moisture and temperature of the litter and cow behaviour (e.g., lying, eating, ruminating). An external weather system and video recording system are also included. The system’s architecture consists of four main elements: sensors, nodes, gateways, and backend. The data are recorded by sensors, then locally processed on custom-developed sensor nodes, and then transmitted via radio channels to local gateways that combine the data from multiple nodes and transmit them to distributed digital storage (“the cloud”) via a 3G/4G cellular network. On the cloud, the data are further processed and stored in a database. The data are then presented to the user continuously and in real time on a dashboard that can be accessed via the internet. In the design of the local wireless network, care was taken to avoid data packet collision and thus to minimize data loss. To test the system’s performance, the system was installed and operated on three commercial dairy cattle farms for one year. The system provided high data stability with minimal loss and outliers, showing that the system is reliable and suitable for long term application on commercial dairy farms. The system’s architecture, communication network, and data processing and visualization applications form an open framework for research and development purposes, allowing it to be customized and fine-tuned before being deployed as a management assistant on commercial dairy farms. Missing elements that should be added in the future are the integration of the data from the milking parlour and cow identification. Algorithms to integrate information from multiple sensors can be added to provide a comprehensive system that monitors all aspects related to cow welfare, health, and production automatically, remotely and in real time, thereby supporting farmers in important management decision-making

Environmental assessment of individual and collective manure management systems

In intensive livestock area with large nutrient surplus collective management systems can be a suitable solution. However, the collective system should carefully evaluated for environmental sustainability to avoid cross effects. The aim of this study was to evaluate the environmental effects of the introduction of a collective treatment plant for energy production and nitrogen removal. For this purpose an assessment methodology, for individual farms and collective treatments plants, has been defined to estimate the emissions of the main pollutants to the air (CO2, CH4, N2O, NH3) and to the soil (N). The method devised has been assessed in a case study (a treatment plant collecting manure from 12 farms). The main effect of the introduction of the collective management system from the environmental point of view is a reduction of greenhouse gases emissions of 61% due to methane emission reduction and renewable energy production. Furthermore, it reduces the amount of nitrogen to be applied to land from 430 kg ha-1 to about 220 kg ha-1, decreases the emission of ammonia in the air by about 17% due to lower amount of nitrogen that is managed by farms in the storage and spreading operations

The Rheumatology drugs for COVID-19 management: Which and when?

Introduction: While waiting for the development of specific antiviral therapies and vaccines to effectively neutralize the SARS-CoV2, a relevant therapeutic strategy is to counteract the hyperinflammatory status, characterized by an increase mainly of interleukin (IL)-1β, IL-2, IL-6, IL-7, IL-8, and tumor necrosis factor (TNF)-α, which hallmarks the most severe clinical cases. 'Repurposing' immunomodulatory drugs and applying clinical management approved for rheumatic diseases represents a game-changer option. In this article, we will review the drugs that have indication in patients with COVID-19, including corticosteroids, antimalarials, anti-TNF, anti-IL-1, anti-IL-6, baricitinib, intravenous immunoglobulins, and colchicine. The PubMed, Medline, and Cochrane Library databases were searched for English-language papers concerning COVID-19 treatment published between January 2020 and October 2020. Results were summarized as a narrative review due to large heterogeneity among studies. In the absence of specific treatments, the use of immunomodulatory drugs could be advisable in severe COVID-19 patients, but clinical outcomes are still suboptimal. An early detection and treatment of the complications combined with a multidisciplinary approach could allow a better recovery of these patient