Wheat Transformation with <i>ScTPS1-TPS2</i> Bifunctional Enzyme for Trehalose Biosynthesis Protects Photosynthesis during Drought Stress

Wheat cultivation makes an important contribution to human nutrition. Trehalose synthesis plays a role in the tolerance to drought stress. A bifunctional TPS-TPP enzyme gene from yeast was used to obtain transgenic wheat plants to increase trehalose synthesis. Mature wheat embryos were transformed using pGreen rd29A::TPS1-TPS2 or pGreen 35S::TPS1-TPS2 constructs. The transgene presence in mature leaves of T3 plants was confirmed by sequencing a PCR fragment of the inserted transgene. Transgenic and NT plants were submitted to drought stress for eight days. Transformed wheat lines retained a higher relative water content than NT plants during drought stress, and the Rubisco activity was unaffected. Plants transformed with the 35S construct showed a lower photosynthetic rate and lower fructose 1–6-bisphosphatase (FBPase) activity during drought, suggesting that constitutive trehalose and sucrose synthesis caused a reduced ribulose 1,5-bisphosphate (RuBP) regeneration. Lines transformed with the rd29A promoter showed a higher photosynthetic rate after eight days of drought, as the RuBP regeneration was unaffected. Transgenic wheat plants had higher biomass and grain weight than NT plants after drought. These results suggest that trehalose synthesis improves photosynthesis during stress and induces changes in the activity of some Calvin-cycle enzymes, reflected in plant metabolism and growth

Efecto prebiótico de los Arabinoxilanos y los Arabinoxilo-Oligosacáridos y su relación con la promoción de la buena salud

Arabinoxylans are polysaccharides present in grains and as such, are part of dietary fiber intake in humans and animals. Enzymatic or chemical hydrolysis of arabinoxylans produces arabinoxilo-oligosaccharides, which can be branched or unbranched with arabinose. The objective of this work was to describe the potential use of arabinoxylans and arabinoxylan-oligosaccharides as prebiotics to promote good health, by selective enhancement of beneficial colonic microbiota growth and metabolic activity. The information generated indicates that arabinoxylans and arabinoxylan-oligosaccharides act by modifying the microbiota selectively and stimulate the biological response favoring good health in the host, by antio-obesity effect, glucose regulator, antioxidant, anticancer, immunomodulator, with similar or better results than recognized prebiotics. However, it is necessary to expand the knowledge we have about arabinoxylans in order to support their application in the food, pharmaceutical, and biomedical industry.Los arabinoxilanos son polisacáridos presentes en los granos de los cereales, y como tales, forman parte de la fibra dietética consumida por humanos y animales. La hidrólisis química o enzimática de los arabinoxilanos produce arabinoxilo-oligosacáridos, los cuales pueden estar ramificados o no, con arabinosa. El objetivo de este trabajo fue exponer el uso potencial de los arabinoxilanos y arabinoxilo-oligosacáridos, como prebióticos, y el efecto de su consumo en la promoción de la buena salud, al estimular selectivamente el crecimiento y actividad metabólica de la microbiótica colónica benéfica. La información generada indica que los arabinoxilanos y arabinoxilo-oligosacáridos actúan modificando la microbiota de manera selectiva, y estimulan la respuesta biológica, favoreciendo la buena salud del hospedero, por su efecto antiobesogénico, regulador de la glucosa, antioxidante, anticancerígeno e inmunomodulador, con resultados similares o mejores en relación a prebióticos reconocidos. No obstante, es necesario ampliar el conocimiento que se tiene de ellos para sustentar su aplicación en la industria alimentaria, farmacéutica o biomédica

Trametes sp. as a Source of Biopolymer Cross-Linking Agents: Laccase Induced Gelation of Ferulated Arabinoxylans

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The Effects of Brief Heat During Early Booting on Reproductive, Developmental, and Chlorophyll Physiological Performance in Common Wheat (Triticum aestivum L.)

Rising temperatures due to climate change threaten agricultural crop productivity. As a cool-season crop, wheat is heat-sensitive, but often exposed to high temperatures during the cultivation period. In the current study, a bread wheat panel of spring wheat genotypes, including putatively heat-tolerant Australian and CIMMYT genotypes, was exposed to a 5-day mild (34°C/28°C, day/night) or extreme (37°C/27°C) heat stress during the sensitive pollen developmental stage. Worsening effects on anther morphology were observed, as heat stress increased from mild to extreme. Even under mild heat, a significant decrease in pollen viability and number of grains per spike from primary spike was observed compared with the control (21°C/15°C), with Sunstar and two CIMMYT breeding lines performing well. A heat-specific positive correlation between the two traits indicates the important role of pollen fertility for grain setting. Interestingly, both mild and extreme heat induced development of new tillers after the heat stress, providing an alternative sink for accumulated photosynthates and significantly contributing to the final yield. Measurements of flag leaf maximum potential quantum efficiency of photosystem II (Fv/Fm) showed an initial inhibition after the heat treatment, followed by a full recovery within a few days. Despite this, model fitting using chlorophyll soil plant analysis development (SPAD) measurements showed an earlier onset or faster senescence rate under heat stress. The data presented here provide interesting entry points for further research into pollen fertility, tillering dynamics, and leaf senescence under heat. The identified heat-tolerant wheat genotypes can be used to dissect the underlying mechanisms and breed climate-resilient wheat

Data on antioxidant activity in grapevine (Vitis vinifera L.) following cryopreservation by vitrification

Cryopreservation is used for the long-term conservation of plant genetic resources. This technique very often induces lethal injury or tissue damage. In this study, we measured indicators of viability and cell damage following cryopreservation and vitrification-cryopreservation in Vitis vinifera L. axillary buds cv. “Flame seedless” stored in liquid nitrogen (LN) for: three seconds, one hour, one day, one week and one month; after LN thawed at 38 °C for three minutes. The enzymatic activity of catalase (CAT) and superoxide dismutase (SOD), as well as the amount of malondialdehyde (MDA), total protein and viability were assayed

Factores de riesgo asociados a diabetes mellitus en la población adulta en cuatro estratos socioeconómicos del municipio de Guatemala

Describir el comportamiento epidemiológico de los factores de riesgo asociados a Diabetes Mellitus tipo 2,prevalentes en la población mayor de 18 años; en los cuatro estratos socioeconómicos IV, III, II, I; determinados por cuatro sectores cartográficos dentro del Municipio de Guatemala, durante el mes de abril del 2015. Estudio descriptivo, transversal donde se utilizó información recabada por medio del instrumento del método progresivo (STEPS) de la Organización Panamericana de la Salud (OPS) modificado para la vigilancia de los factores de riesgo de las enfermedades crónicas no transmisibles en personas mayores de 18 años que residen en viviendas ubicadas dentro de 4 sectores cartográficos, socioeconómicos: alto (IV), medio alto (III), medio bajo (II) y bajo (I); dentro del Municipio de Guatemala. Se incluyeron 223 personas encuestadas de la Ciudad Capital, en donde el estrato IV presentó el mayor porcentaje para tabaco con 16.7% y para consumo perjudicial de alcohol con 65% El estrato III reflejó alta incidencia de Presión Arterial Media con 6%. El sedentarismo, IMC e Índice Cintura Cadera, predominaron en elestrato II quien presentó el mayor porcentaje, con 53, 40 y 53% respectivamente. El factor de riesgo modificable con mayor prevalencia en el estudio fue sedentarismo el cual afecta a un 92% de la población. La población de 21 a 30 años es la que más factores de riesgo asociados a Diabetes Mellitus tipo 2 presenta y el sexo más afectado es el femenino