Os arranjadores da Rádio Record de São Paulo, 1928-1965

História da Rádio Record de São Paulo por meio do repertório musical executado ao vivo em seu auditório. O período abordado neste estudo foi determinado pela cronologia das fontes primárias, de 1928 até 1965, desde a fundação da Rádio Record até a última datação das 3.216 partituras impressas e manuscritas de arranjos e composições originais utilizados pela orquestra da emissora. Esse repertório musical, composto de canções com arranjos orquestrais, se insere na chamada “era de ouro do rádio”. Este estudo apresenta uma seção de referência contendo verbetes sobre arranjadores, músicos, cantores e conjuntos instrumentais; e inclui uma reflexão sobre o conceito de arranjo, gênero, instrumentação e orquestração

Características da personalidade de pais incestuosos por meio do Rorschach, conforme o Sistema Compreensivo

Adotando-se os critérios legais de estupro (artigo 213 do Código Penal Brasileiro) como delimitações para essa conduta ou sintoma interpessoal, a finalidade desse estudo foi a de investigar características da personalidade de indivíduos responsabilizados criminalmente por estupros incestuosos. Participaram do estudo três adultos do sexo masculino, com semelhantes condições sociais, demográficas e educacionais, presos em uma penitenciária do estado de São Paulo pelos estupros das filhas biológicas. Os dados foram coletados por meio de entrevista e do Método de Rorschach, no Sistema Compreensivo de Exner (2003). Para a análise dos resultados foram selecionadas as seguintes variáveis: defensividade, autopercepção, capacidade de autocontrole, relacionamentos e percepção interpessoal, afeto e processos cognitivos aplicados na adaptação à realidade social. Ainda que estupradores não se constituam como um grupo homogêneo e que não haja um padrão de características universais que os represente, os principais resultados permitiram constatar a insuficiência de recursos subjetivos aplicados à percepção de si mesmos e à percepção do outro, dados que foram convergentes com os comumente retratados na literatura sobre psicologia de agressores sexuais.Taking into consideration the legal criteria related to rape (Brazilian Penal Code) as guidelines for this kind of conduct or interpersonal symptom, the objective of this study was to investigate the personality characteristics of individuals criminally responsible for incestuous raping. Participants were three male inmates, with similar social, demographic and educational levels, imprisoned at a State correctional institution in São Paulo for raping their biological daughters. The data were gathered through interviews and Rorschach Method, as Exner's (2003) Comprehensive System. To the analysis of the results the following variables were selected: defensiveness, self-perception, capacity for self-control, relationships and interpersonal perception, affect, and cognitive processing applied to social reality adaptation. Even though rapists do not constitute a homogeneous group and there are no standard universal characteristics that represent them, the main results showed evidence of their limitation on subjective resources applied to self-perception and perception of the others. It was convergent with common data found in the literature regarding the psychology of sexual aggressors

Nuclear Factor of Activated T Cells-dependent Down-regulation of the Transcription Factor Glioma-associated Protein 1 (GLI1) Underlies the Growth Inhibitory Properties of Arachidonic Acid

Numerous reports have demonstrated a tumor inhibitory effect of polyunsaturated fatty acids (PUFAs). However, the molecular mechanisms modulating this phenomenon are in part poorly understood. Here, we provide evidence of a novel antitumoral mechanism of the PUFA arachidonic acid (AA). In vivo and in vitro experiments showed that AA treatment decreased tumor growth and metastasis, and increased apoptosis. Molecular analysis of this effect showed significantly reduced expression of a subset of antiapoptotic proteins, including BCL2, BFL1/A1 and 4-1BB, in AA-treated cells. We demonstrated that downregulation of the transcription factor GLI1 in AA-treated cells is the underlying mechanism controlling BCL2, BFL1/A1 and 4-1BB expression. Using luciferase reporters, chromatin immunoprecipitation, and expression studies, we found that GLI1 binds to the promoter of these antiapoptotic molecules, and regulates their expression and promoter activity. We provide evidence that AA-induced apoptosis and downregulation of antiapoptotic genes can be inhibited by overexpressing GLI1 in AA-sensitive cells. Conversely, inhibition of GLI1 mimics AA treatments, leading to decreased tumor growth, cell viability and expression of antiapoptotic molecules. Further characterization showed that AA represses GLI1 expression by stimulating NFATc1 nuclear translocation, which then binds the GLI1 promoter and represses its transcription. AA was shown to increase reactive oxygen species. Treatment with antioxidants reduced the AA-induced apoptosis, downregulation of GLI1 and NFATc1 activation, indicating that NFATc1 activation and GLI1 repression require the generation of reactive oxygen species. Collectively, these results define a novel mechanism underlying AA antitumoral functions that may serve as a foundation for the future PUFA-based therapeutic approaches

Klinefelter Syndrome at differents ages: multicentric experience

El Síndrome de Klinefelter (SK) es la anormalidad cromosómica más frecuente en los varones, con una prevalencia estimada de 1:600 recién nacidos. El objetivo de este trabajo fue establecer las distintas características de presentación del SK a distintas edades, incluyendo signos y síntomas clínicos, parámetros de laboratorio y otros exámenes complementarios. La franja etaria más frecuente de diagnóstico de SK fue entre los 11 y 20 años (46,8%). En 4 casos el diagnóstico fue prenatal. Los motivos de consulta más frecuentes en forma global fueron la presencia de testículos pequeños, infertilidad y criptorquidia. El cariotipo más prevalente fue el clásico 47,XXY (83,7%), seguido del mosaico 47,XXY/46,XY (7,1%). El promedio de talla de nuestros pacientes prepuberales no mostró diferencia con la población general. Por otro lado, los pacientes puberales presentaron un promedio de talla significativamente más alto, hallándose alrededor de 1 SDS. Hubo correlación entre la edad y el SDS de talla. La media de talla de los adultos fue 178,8 ± 9,0 cm; se observó un 62,5% de sobrepeso/obesidad (IMC ≥ 25,0 kg/m2 ). El 50% de nuestros pacientes con SK menores de 18 años presentaron trastornos neurocognitivos. El hallazgo clínico más frecuente entre los pacientes prepuberales fue la criptorquidia. En los puberales las consultas y hallazgos clínicos más frecuentes fueron: testículos pequeños, criptorquidia y ginecomastia. Todos nuestros pacientes en estadio de Tanner igual o mayor de III presentaron testículos más pequeños para su grado de desarrollo. Los valores de FSH y LH fueron normales en los pacientes prepuberales y comenzaron a aumentar en la pubertad. Los adultos consultaron más frecuentemente por hipotrofia testicular, infertilidad y en menor grado ginecomastia. Todos los pacientes presentaron testículos hipotróficos, con una mediana de volumen testicular de 3,5 (1-8) ml. El 56,4% presentaron función sexual normal; el resto tuvo algún tipo de disfunción sexual. La testosterona total (TT) fue normal en 45% de los pacientes, con descenso consistente con la edad, donde todos los pacientes mayores de 40 años presentaron TT subnormal. El 10,7% de los pacientes que efectuaron espermograma tuvo oligospermia severa, el resto presentó azoospermia. La densitometría ósea fue anormal en el 46,4% de los adultos estudiados. Sin embargo, no hubo diferencias significativas en la prevalencia de osteopenia y osteoporosis entre los pacientes con TT normal o subnormal.Klinefelter syndrome (KS) is the most common chromosomal aberration among men, with an estimated prevalence of 1:600 newborns. It is an X chromosome polysomy, with X disomy being the most common variant (47,XXY). The aim of this study was to establish the characteristics of KS presentation at different ages, including signs and symptoms, laboratory parameters and other diagnostic tests. The diagnosis of KS was more frequent in the age group between 11 and 20 years (46.8%). Most of the patients (83.7%) showed the classic 47,XXY karyotype and 7.1% showed a 47,XXY/46,XY mosaicism. While mean prepubertal height was not different from the control population, it was significantly higher at puberty. Patients consulted most frequently for small testes, infertility and cryptorchidism. In four cases the diagnosis was prenatal. 50% of our patients younger than 18 years presented neurocognitive disorders. The more frequent clinical findings were cryptorchidism in prepubertal patients; small testes, cryptorchidism and gynecomastia in pubertal patients. All our patients in Tanner stage III or more presented small testes. FSH and LH levels were normal in prepubertal patients and increased abnormally at puberty. On the other hand, most adults consulted for small testes, infertility and gynecomastia. 43.6% of patients had decreased libido, sexual and/or ejaculatory dysfunction. In adults average height (178.8 ± 9.0 cm) and weight (83.6 ± 21.0 kg), were higher than in the normal population, however 8 patients (19%) had a height less tan 170 cm. There was 62.5% of overweight / obesity (BMI ≥ 25.0 kg/m2 ) in the whole group of adult patients. 35.2% had eunuchoid proportions. All patients had testicular hypotrophc, with a median testicular volume of 3.5 ml (range 1-8 ml). Total testosterone (TT) levels were normal in 45% of adult patients, showing significant correlation with age. All patients aged 40 or more years had subnormal TT levels. In patients who underwent semen analysis, severe oligospermia and azoospermia were found in 10.7% and 89.3% respectively. Bone mineral densitometry showed low bone mass in 46.4% of cases. No significant differences in the prevalence of osteopenia and osteoporosis were observed among patients with normal or subnormal TT.Fil: Pacenza, Carina Natalia. Unidad Asistencial "Dr. César Milstein"; ArgentinaFil: Pasqualini, T.. Hospital Italiano. Departamento de Pediatria; ArgentinaFil: Gottlieb, Silvia Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; Argentina. Gobierno de la Ciudad Autonoma de Buenos Aires. Hospital General de Niños "ricardo Gutierrez". Departamento de Medicina.; ArgentinaFil: Knoblovits, P.. Hospital Italiano; ArgentinaFil: Costanzo Caso, Pablo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Italiano; ArgentinaFil: Stewart Usher, J.. Centro Médico Haedo. Consultorio de Endocrinología ; ArgentinaFil: Rey, Rodolfo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; Argentina. Gobierno de la Ciudad Autonoma de Buenos Aires. Hospital General de Niños "ricardo Gutierrez". Departamento de Medicina.; ArgentinaFil: Martínez, M.. Gobierno de la Ciudad Autonoma de Buenos Aires. Hospital General de Agudos Carlos Durand.; ArgentinaFil: Aszpis, Sergio Mario. Gobierno de la Ciudad Autonoma de Buenos Aires. Hospital General de Agudos Carlos Durand.; Argentin

Poly (A)+ Transcriptome Assessment of ERBB2-Induced Alterations in Breast Cell Lines

We report the first quantitative and qualitative analysis of the poly (A)+ transcriptome of two human mammary cell lines, differentially expressing (human epidermal growth factor receptor) an oncogene over-expressed in approximately 25% of human breast tumors. Full-length cDNA populations from the two cell lines were digested enzymatically, individually tagged according to a customized method for library construction, and simultaneously sequenced by the use of the Titanium 454-Roche-platform. Comprehensive bioinformatics analysis followed by experimental validation confirmed novel genes, splicing variants, single nucleotide polymorphisms, and gene fusions indicated by RNA-seq data from both samples. Moreover, comparative analysis showed enrichment in alternative events, especially in the exon usage category, in ERBB2 over-expressing cells, data indicating regulation of alternative splicing mediated by the oncogene. Alterations in expression levels of genes, such as LOX, ATP5L, GALNT3, and MME revealed by large-scale sequencing were confirmed between cell lines as well as in tumor specimens with different ERBB2 backgrounds. This approach was shown to be suitable for structural, quantitative, and qualitative assessment of complex transcriptomes and revealed new events mediated by ERBB2 overexpression, in addition to potential molecular targets for breast cancer that are driven by this oncogene

Minimal invasive periodontal regeneration with buccal approach: systematic review

Objective: The aim of this systematic review was to explore the efficacy of different minimal invasive surgical technique (MIST), modified minimal invasive surgical technique (M-MIST) and entire papilla preservation (EPP) approaches for the treatment of intra-bony defect in terms of clinical attachment level (CAL) gain and periodontal pocket depth (PPD) reduction and gengival recession (REC) searching with one shows to have better results and less trauma for the patients. Methods: A detailed review protocol was designed according to PRISMA guideline. Online search was conducted on PubMed, Cochrane Library and Embase. Only randomized clinical trials (RCTs), case series (CSs) and controlled studies (CTs)with a minimum of 10 patients reporting clinical, radiographic and/or patient related outcomes at least, testing MIST or MMIST or EPP procedure, with or without the application of a regenerative tool for the treatment of intra-bony defect and only with buccal incision were included. Results: According to the inclusion and exclusion criteria applied to the research, 12 articles came out and they are the results of this work. These 12 articles were evaluated according to the risk of bias (low, not clear, high or not applicable) and finally showed positive values according to the various techniques studied

Determinants of grandparent-grandchild digital contact in Italy

The broad definition of intergenerational contact includes not only meeting face-to-face, but also the important element of communication at a distance, such as via telephone. With the pervasion of the Internet and electronic devices, digital contact has become another increasingly important option. We examined digital contact between grandparents and grandchildren in comparison with traditional forms of contact (i.e. face-to-face and by telephone) using Italian data from the 2016 Families, Social Subjects and life cycle (FSS) survey. We found that grandparents belonging to younger cohorts, those with higher education, those who lived in urban areas and those in better health were more likely to maintain digital contact with their grandchildren. Results also show that digital contact tends to compensate for a lack of (frequent) face-to-face contact, and to cumulate with telephone contact. Our results have significant implications for the current and future development of intergenerational relationships as they suggest an increasing role of digital contact for distant geographical situations. Against the background of persisting inequalities in the access and the use of the Internet, our findings emphasise the need to improve digital network connectivity and user skills, particularly among specific sub-groups of the population

Bright-Field Multiplex Immunohistochemistry Assay for Tumor Microenvironment Evaluation in Melanoma Tissues

The tumor microenvironment (TME) plays a crucial role in melanoma development, progression and response to treatment. As many of the most relevant TME cell phenotypes are defined by the simultaneous detection of more than two markers, the bright-field (BF) multiplex immunohistochemistry (IHC) technique has been introduced for the quantitative assessment and evaluation of the relative spatial distances between immune cells and melanoma cells. In the current study, we aimed to validate BF multiplex IHC techniques in the Ventana Discovery Ultra Immunostainer to be applied to the evaluation of the TME in variably pigmented melanoma tissues. The BF multiplex IHC staining was performed using different combinations of six immune-cell markers—CD3, CD4, CD8, CD20, CD68 and CD163—and the melanoma cell marker SOX10. Our results show that the BF double IHC Yellow/Purple protocol guarantees the maximum contrast in all the cell populations tested and the combination SOX10 (Green), CD8 (Yellow) and CD163 (Purple) of the BF triple IHC protocol ensures the best contrast and discrimination between the three stained cell populations. Furthermore, the labeled cells were clearly distinct and easily identifiable using the image analysis software. Our standardized BF IHC multiplex protocols can be used to better assess the immune contexts of melanoma patients with potential applications to drive therapeutic decisions within clinical trials