Transcriptome of pleuropodia from locust embryos supports that these organs produce enzymes enabling the larva to hatch

Abstract: Background: Pleuropodia are limb-derived glandular organs that transiently appear on the first abdominal segment in embryos of insects from majority of “orders”. They are missing in the genetic model Drosophila and little is known about them. Experiments carried out on orthopteran insects 80 years ago indicated that the pleuropodia secrete a “hatching enzyme” that digests the serosal cuticle to enable the larva to hatch, but evidence by state-of-the-art molecular methods is missing. Results: We used high-throughput RNA-sequencing to identify the genes expressed in the pleuropodia of the locust Schistocerca gregaria (Orthoptera). First, using transmission electron microscopy we studied the development of the pleuropodia during 11 stages of the locust embryogenesis. We show that the glandular cells differentiate and start secreting just before the definitive dorsal closure of the embryo and the secretion granules outside the cells become more abundant prior to hatching. Next, we generated a comprehensive embryonic reference transcriptome for the locust and used it to study genome wide gene expression across ten morphologicaly defined stages of the pleuropodia. We show that when the pleuropodia have morphological markers of functional organs and produce secretion, they are primarily enriched in transcripts associated with transport functions. They express genes encoding enzymes capable of digesting cuticular protein and chitin. These include the potent cuticulo-lytic Chitinase 5, whose transcript rises just before hatching. Unexpected finding was the enrichment in transcripts for immunity-related enzymes. This indicates that the pleuropodia are equipped with epithelial immunity similarly as barrier epithelia in postembryonic stages. Conclusions: These data provide transcriptomic support for the historic hypothesis that pleuropodia produce cuticle-degrading enzymes and function in hatching. They may also have other functions, such as facilitation of embryonic immune defense. By the genes that they express the pleuropodia are specialized embryonic organs and apparently an important though neglected part of insect physiology

Examining Mechanisms of Childhood Cognitive Control

Childhood cognitive control is an important predictor for positive development, yet interventions seeking to improve it have provided mixed results. This is partly due to lack of clarity surrounding mechanisms of cognitive control, notably the role of inhibition and context monitoring. Here we use a randomized controlled trial to causally test the contributions of inhibition and context monitoring to cognitive control in childhood. Sixty children aged 6 to 9-years were assigned to three groups training either inhibition, context monitoring group or response speed using a gamified, highly variable and maximally adaptive training protocol. Whereas all children improved in the targeted cognitive functions over the course of training, pre-post data show that only the inhibition group improved on cognitive control. These findings serve as a first step in demonstrating the promise inhibition-based cognitive control interventions may hold

Transcriptome of pleuropodia from locust embryos supports that these organs produce enzymes enabling the larva to hatch.

Background:Pleuropodia are limb-derived glandular organs that transiently appear on the first abdominal segment in embryos of insects from majority of "orders". They are missing in the genetic model Drosophila and little is known about them. Experiments carried out on orthopteran insects 80 years ago indicated that the pleuropodia secrete a "hatching enzyme" that digests the serosal cuticle to enable the larva to hatch, but evidence by state-of-the-art molecular methods is missing. Results:We used high-throughput RNA-sequencing to identify the genes expressed in the pleuropodia of the locust Schistocerca gregaria (Orthoptera). First, using transmission electron microscopy we studied the development of the pleuropodia during 11 stages of the locust embryogenesis. We show that the glandular cells differentiate and start secreting just before the definitive dorsal closure of the embryo and the secretion granules outside the cells become more abundant prior to hatching. Next, we generated a comprehensive embryonic reference transcriptome for the locust and used it to study genome wide gene expression across ten morphologicaly defined stages of the pleuropodia. We show that when the pleuropodia have morphological markers of functional organs and produce secretion, they are primarily enriched in transcripts associated with transport functions. They express genes encoding enzymes capable of digesting cuticular protein and chitin. These include the potent cuticulo-lytic Chitinase 5, whose transcript rises just before hatching. Unexpected finding was the enrichment in transcripts for immunity-related enzymes. This indicates that the pleuropodia are equipped with epithelial immunity similarly as barrier epithelia in postembryonic stages. Conclusions:These data provide transcriptomic support for the historic hypothesis that pleuropodia produce cuticle-degrading enzymes and function in hatching. They may also have other functions, such as facilitation of embryonic immune defense. By the genes that they express the pleuropodia are specialized embryonic organs and apparently an important though neglected part of insect physiology

Transcriptome of pleuropodia from locust embryos supports that these organs produce enzymes enabling the larva to hatch

Abstract: Background: Pleuropodia are limb-derived glandular organs that transiently appear on the first abdominal segment in embryos of insects from majority of “orders”. They are missing in the genetic model Drosophila and little is known about them. Experiments carried out on orthopteran insects 80 years ago indicated that the pleuropodia secrete a “hatching enzyme” that digests the serosal cuticle to enable the larva to hatch, but evidence by state-of-the-art molecular methods is missing. Results: We used high-throughput RNA-sequencing to identify the genes expressed in the pleuropodia of the locust Schistocerca gregaria (Orthoptera). First, using transmission electron microscopy we studied the development of the pleuropodia during 11 stages of the locust embryogenesis. We show that the glandular cells differentiate and start secreting just before the definitive dorsal closure of the embryo and the secretion granules outside the cells become more abundant prior to hatching. Next, we generated a comprehensive embryonic reference transcriptome for the locust and used it to study genome wide gene expression across ten morphologicaly defined stages of the pleuropodia. We show that when the pleuropodia have morphological markers of functional organs and produce secretion, they are primarily enriched in transcripts associated with transport functions. They express genes encoding enzymes capable of digesting cuticular protein and chitin. These include the potent cuticulo-lytic Chitinase 5, whose transcript rises just before hatching. Unexpected finding was the enrichment in transcripts for immunity-related enzymes. This indicates that the pleuropodia are equipped with epithelial immunity similarly as barrier epithelia in postembryonic stages. Conclusions: These data provide transcriptomic support for the historic hypothesis that pleuropodia produce cuticle-degrading enzymes and function in hatching. They may also have other functions, such as facilitation of embryonic immune defense. By the genes that they express the pleuropodia are specialized embryonic organs and apparently an important though neglected part of insect physiology

Transcriptome of pleuropodia from locust embryos supports that these organs produce enzymes enabling the larva to hatch

Abstract: Background: Pleuropodia are limb-derived glandular organs that transiently appear on the first abdominal segment in embryos of insects from majority of “orders”. They are missing in the genetic model Drosophila and little is known about them. Experiments carried out on orthopteran insects 80 years ago indicated that the pleuropodia secrete a “hatching enzyme” that digests the serosal cuticle to enable the larva to hatch, but evidence by state-of-the-art molecular methods is missing. Results: We used high-throughput RNA-sequencing to identify the genes expressed in the pleuropodia of the locust Schistocerca gregaria (Orthoptera). First, using transmission electron microscopy we studied the development of the pleuropodia during 11 stages of the locust embryogenesis. We show that the glandular cells differentiate and start secreting just before the definitive dorsal closure of the embryo and the secretion granules outside the cells become more abundant prior to hatching. Next, we generated a comprehensive embryonic reference transcriptome for the locust and used it to study genome wide gene expression across ten morphologicaly defined stages of the pleuropodia. We show that when the pleuropodia have morphological markers of functional organs and produce secretion, they are primarily enriched in transcripts associated with transport functions. They express genes encoding enzymes capable of digesting cuticular protein and chitin. These include the potent cuticulo-lytic Chitinase 5, whose transcript rises just before hatching. Unexpected finding was the enrichment in transcripts for immunity-related enzymes. This indicates that the pleuropodia are equipped with epithelial immunity similarly as barrier epithelia in postembryonic stages. Conclusions: These data provide transcriptomic support for the historic hypothesis that pleuropodia produce cuticle-degrading enzymes and function in hatching. They may also have other functions, such as facilitation of embryonic immune defense. By the genes that they express the pleuropodia are specialized embryonic organs and apparently an important though neglected part of insect physiology

The process architecture of memory in early to middle childhood

Das Gedächtnis ist kein einheitliches Konstrukt, sondern beruht auf unterschiedlichen Wirkgrößen, die es uns erlauben, scheinbar gegensätzliche Anforderungen zu erfüllen. Ein interessantes Spannungsfeld stellt dabei der Kontrast zwischen der Notwendigkeit dar, Gemeinsamkeiten zwischen Ereignissen zu extrahieren und anzuwenden (Generalisierung) und separate Repräsentationen von ähnlichen Ereignissen zu formen (Spezifität). In dieser Arbeit wurde die Gedächtnisarchitektur in der Kindheit mit einem Prozess-orientierten Ansatz untersucht. Basierend auf einem Literatur Review und empirischer Daten deuten die Ergebnisse auf eine unterschiedliche Entwicklung verschiedener Gedächtnisprozesse in der frühen bis mittleren Kindheit hin. Weiterhin präsentiert diese Arbeit ein Multi-Indikatoren-Studiendesign zur Untersuchung der zugrundeliegenden Faktorenstruktur des Gedächtnisses in der Kindheit und adressiert methodische Herausforderungen für den Vergleich konkurrierender mathematischer Modelle

On Matching Item Wording of Self and Proxy Reports on the SDQ and Its Effects on Cross-Informant Discrepancies

Vierhaus M, Rüth J-E, Buchberger ES, Lohaus A. Zur Angleichung von Itemwortlauten bei SDQ-Fremd- und Selbstbericht und deren Auswirkung auf Beurteilungsdiskrepanzen . DIAGNOSTICA. 2018;64(4):169-179.This study compared the German self and proxy report versions of the Strengths and Difficulties Questionnaire (SDQ) in order to analyze discrepancies between the informants. We compared the original scales with modified scales, which contained more closely matched item wordings. In a sample of 217 mother-child-dyads the original item versions were applied, while 276 dyads were asked to provide reports based on the modified versions. For the self as well as for the proxy reports, the results showed partial measurement invariance between the original and the modified versions of the questionnaire. Moreover, the informant discrepancies between the self and proxy reports were lower for the modified version regarding the means as well as the correlations between self and proxy reports. The results underline the significance of item wordings for questionnaire results in general and, in particular, for the emergence of informant discrepancies

Cloudy with a Chance of Insights: Context Dependent Gene Regulation and Implications for Evolutionary Studies

Research in various fields of evolutionary biology has shown that divergence in gene expression is a key driver for phenotypic evolution. An exceptional contribution of cis-regulatory divergence has been found to contribute to morphological diversification. In the light of these findings, the analysis of genome-wide expression data has become one of the central tools to link genotype and phenotype information on a more mechanistic level. However, in many studies, especially if general conclusions are drawn from such data, a key feature of gene regulation is often neglected. With our article, we want to raise awareness that gene regulation and thus gene expression is highly context dependent. Genes show tissue- and stage-specific expression. We argue that the regulatory context must be considered in comparative expression studies

The process structure of memory abilities in early and middle childhood.

Memories enable the retrieval of specific events in the past while building generalizable knowledge that guides inference in new situations. According to a prominent conceptualization, serving both of these adaptive functions requires pattern separation, pattern completion, and generalization as distinct sets of complementary component processes. It is not known whether these three processes are separable in early childhood or whether they originally represent a single dimension of memorability that differentiates in middle childhood. Based on a selection of13 memory tasks, we will address this question though hypothesis-guided tests of differences in the factor structure of memory performance between younger and older groups of children (two groups, 48–59 versus 72–83 months of age, n = 120per group) using structural equation modeling. The results of this study will pave the way for a process-based and systemic understanding of memory development during childhood