Searching for a continuum 4D field theory arising from a 5D non-abelian gauge theory

The anisotropic 5D SU(2) Yang-Mills model has been widely investigated on the lattice during the last decade. In the case where all dimensions are large in size, it was previously claimed that there is a new phase in the phase diagram, called the Layer phase. In this phase, the gauge fields would be localized on 4D layers. Previous works claim that the phase transition to the Layer phase is of second order, which would allow a continuum limit to be taken. We present the extension of the previous work to large lattices, for which we found a first order phase transition. This leaves the scenario that this 5D theory can be dimensionally reduced to a continuum 4D field theory, doubtful.Comment: 6 pages, 2 figures - talk presented at the 31st International Symposium on Lattice Field Theory - Lattice 2013, Mainz, German

Development of EIS cell chips and their application for cell analysis

International audience; We report the development of EIS cell chips able to monitor cell growth and adhesion. They are made of transparent or semitransparent materials to allow complementary analysis of cell behaviour during the measurements through optical microscopy. Our approach is cheap both in fabrication and usage, it is not invasive for cells and it does not require any additional reagent. Our devices are particular suitable to count cells or to evaluate cell morphology and changes as a consequence of different treatments

Transistors based on the Guanosine molecule (a DNA base)

Abstract Molecules are attractive to develop nano-electronic devices. In this paper a new type of transistor is realized by using self-organized films of the Guanosine molecule, a modified DNA base. With its 40 nm channel length the transistor is a good starting point for a new class of nano-electronics devices. Experimental current-voltage characteristics are shown. A circuital model is also proposed

Use of molecular tools for the diagnosis of rangeliosis by Rangelia vitalii in Argentina: A case report

Vector-borne pathogens are responsible for serious emerging diseases and Rangelia vitalii, the etiologic agent of canine rangeliosis, is one of the most pathogenic tick-borne pathogens for dogs in South America. This protozoan is transmitted by the Amblyomma aureolatum tick bite and the clinical features associated to the disease are fever, hemolytic anemia, jaundice, hepatosplenomegaly and bleeding from natural orifices, mainly from the ear egde. The reports of canine rangeliosis in Argentina are scarce. In the present study we report the detection of Rangelia vitalii in a naturally infected dog from Gualeguay, Entre Ríos, Argentina with history of tick infestation and clinical signs compatible with rangeliosis. An initial blood sample was positive to piroplasmids by blood smear examination and the molecular amplification of a fragment of the 18SrRNA gene. Sequencing of the fragment confirmed the pathogen identity. After treatment with imidocarb dipropionate, the clinical signs remitted and the blood smear tested negative.Instituto de BiotecnologíaFil: Borras, Pablo. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán" (ANLIS). Centro Nacional de Diagnóstico e Investigación en Endemo Epidemias (CENDIE); ArgentinaFil: Salvador, F. Laboratorio MF Salvador; ArgentinaFil: Rinaldi, V. Laboratorio MF Salvador; ArgentinaFil: Armitano, Rita Inés. Instituto Nacional de Enfermedades Infecciosas. Servicio Bacteriología Especial; ArgentinaFil: Armitano, Rita Inés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Laboratorio de Hemoparásitos; Argentina.Fil: Farber, Marisa Diana. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán" (ANLIS). INEI. Servicio Bacteriología Especial; ArgentinaFil: Sanchez, R. ROSLAB Diagnostico Veterinario; ArgentinaFil: Mori, L. Laboratorio MF Salvador; ArgentinaFil: Guillemi, Eliana Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Guillemi, Eliana Carolina. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán" (ANLIS). INEI. Servicio Bacteriología Especial; Argentin

Mini-FLOTAC, Kato-Katz and McMaster: three methods, one goal; highlights from north Argentina

Background: Copro-parasitological diagnosis is still a challenge in management of helminth infections at individualand community levels in resource-limited settings.The aim of our study was to compare the performance of three quantitative techniques: Kato-Katz, McMaster andMini-FLOTAC methids. The study was carried out in Oran, Northern Argentina.Methods: 200 schoolchildren were enrolled to provide a single stool sample, which was tested for helminthinfections with Kato-Katz, McMaster and Mini-FLOTAC methods. The Mini-FLOTAC was performed with two flotationsolutions (FS2 saturated saline and FS7 zinc sulphate). Preparation and reading time for each of the three methodswas calculated both when processing single and multiple samples.Results: Out of 193 schoolchildren examined, 40% were positive for any helminth infection by any method; themost prevalent was Hymenolepis nana (23%) followed by Ascaris lumbricoides (17%) and a third group of lessprevalent helminths: Enterobius vermicularis, Trichuris trichiura and hookworms (11% all together). Mini-FLOTAC FS2was more sensitive than FS7 for H. nana (93% vs 78%) and for other helminths (85% vs 80%), whereas FS7 was moresensitive for A. lumbricoides (87% vs 61%). Kato-Katz method was more sensitive than McMaster method for A.lumbricoides (84% vs 48%) and for other helminths (48% vs 43%) except for H. nana (49% vs 61%). As for eggcounts, Mini-FLOTAC FS2 reported 904 eggs per gram of faeces (EPG) for H. nana (vs 457 with McMaster and 111with Kato-Katz) and 1177 EPG for A. lumbricoides (vs 1315 with Kato-Katz and 995 with McMaster); FS2 detected thehighest EPG for both H.nana and A.lumbricoides (904 vs 568 and 1177 vs 643 respectively), the differences werenot statistically significant. The technique feasibility was calculated: Kato-Katz mean time was 48 minutes/sample,Mini-FLOTAC 13 minutes/sample and McMaster 7 minutes/sample. However, especially for Kato-Katz and Mini-FLOTAC,the mean time (min/sample) decreased significantly when processing multiple samples.Conclusions: Mini-FLOTAC is a promising technique for helminth diagnosis, it is more sensitive than Kato-Katz andMcMaster for H. nana and as sensitive as Kato-Katz and more sensitive than McMaster for A. lumbricoides identification.Egg counts differences although relevant, did not reach statistical significance.Fil: Barda, Beatrice. San Raffaele Hospital; ItaliaFil: Cajal, Pamela. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; ArgentinaFil: Villagran, Eliana. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; ArgentinaFil: Cimino, Ruben. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; ArgentinaFil: Juarez, Marisa. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; ArgentinaFil: Krolewiecki, Alejandro Javier. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; ArgentinaFil: Rinaldi, Laura. Universidad de Napoles Federico III; ItaliaFil: Cringoli, Giuseppe. Universidad de Napoles Federico III; ItaliaFil: Burioni, Roberto. San Raffaele Hospital; ItaliaFil: Albonico, Marco. Ivo de Carneri Foundation; Itali

Field Effect Transistor Based on a Modified DNA Base

In this work, a field effect transistor based on a deoxyguanosine derivative (a DNA base) is demonstrated. Our experiments on transport through the source and drain electrodes interconnected by self-assembled guanine ribbons (Gottarelli et al. Helv. Chim. Acta 1998, 81, 2078; Gottarelli et al. Chem. Eur. J. 2000, 6, 3242; Giorgi et al. Chem Eur. J. 2002, 8, 2143) suggest that these devices behave like p-channel MOSFETs, The devices exhibit a maximum voltage gain of 0.76. This prototype transistor represents a starting point toward the development of biomolecular electronic devices

Hybrid molecular electronic (HME) transistor based on deoxyguanosine derivatives

In this work, a field effect transistor based on deoxyguanosine derivatives (a DNA basis) is demonstrated by means of systematic transport experiments. Our nanodevices were fabricated starting from a deoxyguanosine derivative (dG(C-10)(2)) layer interconnecting planar nano-electrodes, with separation in the 20-40nm range. The three terminal devices exhibit a maximum voltage gain of 0.76. Though the quick aging and the reproducibility of the devices have to be improved, the realization of a transistor-like device represents a starting point towards the development of planar solid-state bio-molecular electronic devices

Nano-scaled Biomolecular Field-Effect Transistors: Prototypes and Evaluations

As modern electronics rapidly approach the ultimate level of integration (typically thought to be at the nanoscale level), the fascinating world of biomolecules provides new opportunities and directions for further miniaturization. In this work we review our results in the field of biomolecular electronics, starting from the fabrication of nanojunctions up to the implementation of hybrid devices

Extracellular ATP hydrolysis in Caco-2 human intestinal cell line

Extracellular nucleotides and nucleosides activate signaling pathways that play major roles in the physiology and pathophysiology of the gastrointestinal tract. Ectonucleotidases hydrolyze extracellular nucleotides and thus regulate ligand exposure to purinergic receptors. In this study, we investigated the expression, localization and activities of ectonucleotidases using Caco-2 cells, a model of human intestinal epithelial cells. In addition, by studying ATP release and the rates of extracellular ATP (eATP) hydrolysis, we analyzed the contribution of these processes to the regulation of eATP in these cells. Results show that Caco-2 cells regulate the metabolism of eATP and by-products by ecto-nucleoside triphosphate diphosphohydrolase-1 and -2, a neutral ecto-phosphatase and ecto-5′-nucleotidase. All these ectoenzymes were kinetically characterized using intact cells, and their presence confirmed by denatured and native gels, western blot and cytoimmunofluorescence techniques. In addition, regulation of eATP was studied by monitoring the dynamic balance between intracellular ATP release and ectoATPase activity. Following mechanical and hypotonic stimuli, Caco-2 cells triggered a strong but transient release of intracellular ATP, with almost no energy cost, leading to a steep increase of eATP concentration, which was later reduced by ectoATPase activity. A data-driven algorithm allowed quantifying and predicting the rates of ATP release and ATP consumption contributing to the dynamic accumulation of ATP at the cell surface.Fil: Schachter, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Alvarez, Cora Lilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Bazzi, Zaher. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Faillace, Maria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Corradi, Gerardo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Hattab, C.. Universite de Paris. Institut National de la Transfusion Sanguine.; FranciaFil: Rinaldi, Debora Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Gonzalez-Lebrero, Rodolfo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Pucci Molineris, Melisa Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: Sévigny, J.. Laval University; CanadáFil: Ostuni, M. A.. Universite de Paris; Francia. Universite Paris D. Diderot - Paris 7. French National Institute Of Blood Transfusion.; FranciaFil: Schwarzbaum, Pablo Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentin

Metalloprotein-based electronic nanodevices

A key challenge of the current research in nanoelectronics is the realization of biomolecular devices. The use of electron-transfer proteins, such as the blue copper protein, azurin (Az), is particularly attractive because of its natural redox properties and self-assembly capability. This chapter dis- cusses results of fabrication, characterization, and modeling of devices based on this redox protein. The prototype of biomolecular devices operate in the solid state and in air. The charge transfer process in protein devices can be engineered by using proteins with different redox centers (metal atoms) and by controlling their orientation in the solid state through different immobilization methods. A biomolecular electron rectifier has been demonstrated by interconnecting two gold nanoelectrodes with an Az monolayer immobilized on SiO2. The device exhibits a clear rectifying behavior with discrete current steps in the positive wing of the current— voltage (I-V) curve, which is ascribed to resonant tunneling through the redox active center. On the basis of these results, an Az-based three- terminal device has been designed. The three-terminal device exhibits an ambipolar behavior as a function of the gate bias, thus opening the way to the implementation of a new generation of logic architectures. This peculiar characteristic allows the implementation of a fully integrated nanoscopic logic gate