Impatto psico-emotivo della sindrome coronarica acuta

Background. Acute coronary syndromes can substantially disrupt the lives of our patients. However, patients\u27 view of their illness is usually scarcely considered in routine medical practice. We aimed to investigate the psycho- emotional impact of acute coronary syndromes. Methods.We studied 30 patients with acute coronary syndrome, aged <70 years. Before discharge, each patient was interviewed by a psychologist using a semi-structured interview; data were collected in the form of verbal reports. Results. After learning that they had had a heart attack, 16 patients (53%) reported a feeling of fear linked to the idea of death, sometimes real despair; 7 patients (23%) showed a weak emotional response, 4 (13%) reacted with denial, and 3 responded evasively. During the interview, 17 patients (57%) reported events relating to family life and 9 (30%) to work; all these events were negative and related to suffering, conflict, and stress. When compared to pre-admission status, 30% of patients experienced negative psychological changes, such as a sense of fragility, uncertainty and a redefinition of self-image, while 50% reported no change. Surprisingly, 6 patients (20%) referred positive psychological changes. This positive restructuring was related to the patients\u27 ability/need to change their lifestyle. This process entails a different vision of the past and the future, and is associated with a feeling of rebirth and physical wellbeing. Conclusions. The psycho-emotional effects of acute coronary syndromes may be immense, and may radically change the way the patient relates to himself and to the world. We often remain detached from these changes, and are sometimes even unaware of them

Iron-Catalyzed Reductive Amination of Aldehydes in Isopropyl Alcohol/Water Media as Hydrogen Sources

Reductive amination can be carried in i-PrOH/H2O as hydrogen sources using commercially available iron carbonyl complexes. Within an aqueous alkaline environment, a hydridocarboferrate is formed and its reducing potential is exploited for hydrogenation of the imine (or iminium ion) obtained in situ from aldehydes or ketones, and primary or secondary amines in almost equimolar ratio. This completely sustainable and hydrogen-free process proceeds at 100 °C using Fe3(CO)12as catalyst precursor under convectional heating while Fe2(CO)9gave better results when the reaction was carried out under MW dielectric heating. Both enolizable and non-enolizable aldehydes may be successfully employed in reactions with aliphatic and aromatic amines. (Figure presented.)

A pH-responsive crosslinker platform for antibody-drug conjugate (ADC) targeting delivery

We report a new 1-6 self-immolative, traceless crosslinker derived from the natural product gallic acid. The linker acts through a pH-dependent mechanism for drug release. This 5-(hydroxymethyl)pyrogallol orthoester derivative (HMPO) was stable for 24 hours at pH values of 7.4 and 6.6 and in plasma, releasing molecules bound to the hydroxymethyl moiety under acid-dependent stimuli at pH 5.5. The linker was non-toxic and was used for the conjugation of Doxorubicin (Doxo) or Combretastatin A4 with Cetuximab. The ADCs formed showed their pH responsivity reducing cell viability of A431 and A549 cancer cells better than Cetuximab alone. © 2022 The Royal Society of Chemistry

Mapping of Yucca gloriosa invasion along Mediterranean coastal dunes using unmanned aerial vehicles (UAVs)

Invasive Alien Plants (IAPs) are defined as those species whose introduction and/or spread outside their natural distribution can threaten the biological diversity of an ecosystem. They represent a severe threat to biodiversity and can severely alter the performance of crucial ecosystems such as coastal dunes. Coastal dunes are composed of specialized fauna and flora, converting them into strategic ecosystems due to their unique ecological functions. Coastal dunes are one of the most invaded European environments, with a total flora composed of up to 7% of IAPs. One of these IAPs is Yucca gloriosa L. (Asparagaceae), a homoploid hybrid native plant from the United States of America introduced to Europe for ornamental purposes. For Italy, Y. gloriosa is a typical invasive species related to fixed dunes dominated by Juniperus sp. pl. (with which it competes). Concerning biological invasions, maybe the most effective management strategy is early detection and mapping. In that sense, this work explores and tests the potential of unmanned aerial vehicles (UAVs) for detecting, mapping, and managing the Y. gloriosa invasion along the Mediterranean coastal dunes of a protected area located in Tuscany (Italy). In detail, this work focuses on four aspects: i) determine the suitability of RGB images to map Y. gloriosa, ii) identify the best time of data acquisition, iii) detect changes affecting the occurrence and cover of Y. gloriosa, and iv) propose a set of management actions to preserve a coastal dune ecosystem subjected to plant invasion. The UAV images allowed the generation of ultra-high spatial resolution maps to assess the Y. gloriosa invasion and distribution along the surveyed sandy dunes. The morphological characteristics of this plant – especially at the foliar level – make the invasion of Y. gloriosa particularly suitable for being evaluated and managed using UAV mapping, especially in coastal dunes vegetation that can be a mosaic of different plant communities hard to identify when airborne or satellite images are used. According to the obtained results, the spring season is the perfect time to collect data because light conditions are ideal, with minimal shading effects. Also, due to the peculiar morphology of Y. gloriosa leaves, the timing of data collection seems not crucial for mapping this IAP on coastal dune habitats. Despite the massive eradication action funded by a LIFE project during the period 2005-2009, Y. gloriosa is still present in the protected area and affects the priority habitat of juniper. This might be linked to the fact that Y. gloriosa is a perennial rhizomatous shrub capable of switching its photosynthesis from the C3 to CAM pathway and, therefore, withstand the harsh environmental conditions of dune ecosystems. The early detection using UAVs is the first line of defense against IAPs colonization over any ecosystem. From a conservation and management point of view, this work suggests the development of annual biomonitoring of Y. gloriosa to check its distribution over time and control its spread where it becomes particularly invasive. In this case, one possible strategy will be to keep the IAP in isolated nuclei of plants that are easier to remove with mechanical methods. In a broader view, the study area and the Mediterranean coast must include the ban on introducing non-native species as a strategy, adopting a short-term non-native Management Plan as urgent action

Antibody drug conjugates with hydroxamic acid cargos for histone deacetylase (HDAC) inhibition.

The bioconjugation of hydroxamic acids to antibodies has been made possible through a non-cleavable linker based on the p-mercaptobenzyl alcohol structure that releases hydroxamates in the cells

Heavily fluorinated carbohydrates as enzyme substrates: oxidation of tetrafluorinated galactose by galactose oxidase

Galactose oxidase (GOase) was shown to oxidise several C2/C3 fluorinated galactose analogues. Interestingly, the enzyme was able to distinguish between the 2,3-tetrafluorinated galactose and its epimeric glucose analogue, and this represents the first reported biotransformation of a heavily fluorinated suga

Impact of landscape configuration and composition on pollinator communities across different European biogeographic regions

IntroductionHeterogeneity in composition and spatial configuration of landscape elements support diversity and abundance of flower-visiting insects, but this is likely dependent on taxonomic group, spatial scale, weather and climatic conditions, and is particularly impacted by agricultural intensification. Here, we analyzed the impacts of both aspects of landscape heterogeneity and the role of climatic and weather conditions on pollinating insect communities in two economically important mass-flowering crops across Europe. MethodsUsing a standardized approach, we collected data on the abundance of five insect groups (honey bees, bumble bees, other bees, hover flies and butterflies) in eight oilseed rape and eight apple orchard sites (in crops and adjacent crop margins), across eight European countries (128 sites in total) encompassing four biogeographic regions, and quantified habitat heterogeneity by calculating relevant landscape metrics for composition (proportion and diversity of land-use types) and configuration (the aggregation and isolation of land-use patches). ResultsWe found that flower-visiting insects responded to landscape and climate parameters in taxon- and crop-specific ways. For example, landscape diversity was positively correlated with honey bee and solitary bee abundance in oilseed rape fields, and hover fly abundance in apple orchards. In apple sites, the total abundance of all pollinators, and particularly bumble bees and solitary bees, decreased with an increasing proportion of orchards in the surrounding landscape. In oilseed rape sites, less-intensively managed habitats (i.e., woodland, grassland, meadows, and hedgerows) positively influenced all pollinators, particularly bumble bees and butterflies. Additionally, our data showed that daily and annual temperature, as well as annual precipitation and precipitation seasonality, affects the abundance of flower-visiting insects, although, again, these impacts appeared to be taxon- or crop-specific. DiscussionThus, in the context of global change, our findings emphasize the importance of understanding the role of taxon-specific responses to both changes in land use and climate, to ensure continued delivery of pollination services to pollinator-dependent crops

Impact of landscape configuration and composition on pollinator communities across different European biogeographic regions

Introduction: Heterogeneity in composition and spatial configuration of landscape elements support diversity and abundance of flower-visiting insects, but this is likely dependent on taxonomic group, spatial scale, weather and climatic conditions, and is particularly impacted by agricultural intensification. Here, we analyzed the impacts of both aspects of landscape heterogeneity and the role of climatic and weather conditions on pollinating insect communities in two economically important mass-flowering crops across Europe. Methods: Using a standardized approach, we collected data on the abundance of five insect groups (honey bees, bumble bees, other bees, hover flies and butterflies) in eight oilseed rape and eight apple orchard sites (in crops and adjacent crop margins), across eight European countries (128 sites in total) encompassing four biogeographic regions, and quantified habitat heterogeneity by calculating relevant landscape metrics for composition (proportion and diversity of land-use types) and configuration (the aggregation and isolation of land-use patches). Results: We found that flower-visiting insects responded to landscape and climate parameters in taxon- and crop-specific ways. For example, landscape diversity was positively correlated with honey bee and solitary bee abundance in oilseed rape fields, and hover fly abundance in apple orchards. In apple sites, the total abundance of all pollinators, and particularly bumble bees and solitary bees, decreased with an increasing proportion of orchards in the surrounding landscape. In oilseed rape sites, less-intensively managed habitats (i.e., woodland, grassland, meadows, and hedgerows) positively influenced all pollinators, particularly bumble bees and butterflies. Additionally, our data showed that daily and annual temperature, as well as annual precipitation and precipitation seasonality, affects the abundance of flower-visiting insects, although, again, these impacts appeared to be taxon- or crop-specific. Discussion: Thus, in the context of global change, our findings emphasize the importance of understanding the role of taxon-specific responses to both changes in land use and climate, to ensure continued delivery of pollination services to pollinator-dependent crops

Large scale multifactorial likelihood quantitative analysis of BRCA1 and BRCA2 variants: An ENIGMA resource to support clinical variant classification

The multifactorial likelihood analysis method has demonstrated utility for quantitative assessment of variant pathogenicity for multiple cancer syndrome genes. Independent data types currently incorporated in the model for assessing BRCA1 and BRCA2 variants include clinically calibrated prior probability of pathogenicity based on variant location and bioinformatic prediction of variant effect, co-segregation, family cancer history profile, co-occurrence with a pathogenic variant in the same gene, breast tumor pathology, and case-control information. Research and clinical data for multifactorial likelihood analysis were collated for 1,395 BRCA1/2 predominantly intronic and missense variants, enabling classification based on posterior probability of pathogenicity for 734 variants: 447 variants were classified as (likely) benign, and 94 as (likely) pathogenic; and 248 classifications were new or considerably altered relative to ClinVar submissions. Classifications were compared with information not yet included in the likelihood model, and evidence strengths aligned to those recommended for ACMG/AMP classification codes. Altered mRNA splicing or function relative to known nonpathogenic variant controls were moderately to strongly predictive of variant pathogenicity. Variant absence in population datasets provided supporting evidence for variant pathogenicity. These findings have direct relevance for BRCA1 and BRCA2 variant evaluation, and justify the need for gene-specific calibration of evidence types used for variant classification