Sarcoid Associated with Infection by Habronema spp. in Equines in Brazil

Background: Equine sarcoid, supposed to be caused by infection with bovine papillomavirus type 1 or 2, is usually observedin previously traumatized skin areas, including lesions of habronemosis due to inoculation of third stage larvae in ulcerated wounds by Musca domestica or Stomoxys calcitrans. Little is known about the occurrence of diseases associated with equine sarcoid, mainly because limitations on clinical diagnosis, due to the different skin diseases that have to beconsidered as differential diagnoses. This report aimed to describe three cases of equine sarcoid associated with habronemosis in horses in the semiarid region of Northeastern Brazil.Cases: Three cases of sarcoid associated with habronemosis in equines were diagnosed at the Animal Pathology Laboratory of the Veterinary Hospital of the Federal University of Campina Grande, Patos, Paraíba. Case 1. A 5-year-old female showed in the ventral branch of the mandible a nodule of 3 cm in diameter, partially covered with skin and hair intercepted by areas of irregular surface with yellow-red ulcerations. The cut surface was formed by whitish and firm tissue. Case 2. It was a biopsy from a 4-year-old mare, who was not informed of the macroscopic characteristics of the lesion. Case 3. A 5-year-old horse presenting a nodular mass in the region of the tarsal-metatarsal joint, measuring 8.0x5.0x3.0 cm with an irregular, ulcerated, red-blackish surface. The cut surface was firm and whitish with brownish punctate areas. Microscopicallyall the lesions were classified as equine sarcoid of mixed type with abundant collagen fibers and randomly extensive proliferation of fibroblasts in the dermis. These fibroblasts had an elongated and weakly eosinophilic cytoplasm, rounded nucleus and prominent nucleoli. There were low mitotic activity. Hyperplasia, hyperkeratosis and sometimes ulcerated areas covered by serous cellular scabs were observed in the skin. Multifocal coalescing, granulomatous and eosinophilic lesions were observed within the neoplastic tissue. Cylindrical structures with an elongated thick eosinophilic outer cuticle and obvious side spicules, morphologically compatible with larvae Habronema spp, surrounded by inflammatory cells and cellular debris were observed in Cases 1 and 2. In case 3, intralesional larvae were not observed, but histologic lesions had a similar pattern than cases 1 and 2.Discussion: In these cases the affected animals presented simultaneously a mixed lesion of sarcoid and habronemosis, which leads to complications in clinical diagnosis and difficulties to institute appropriate therapy. Histopathological examination of such lesions is necessary because should characterize their morphology and the causative agent, discarding the other differential diagnoses. The combination of these two conditions can probably be related to the fact that sarcoid may develop up in places previously traumatized, such us lesions of habronemosis. It is important to differentiate these lesions from other skin diseases such as granulation tissue, pythiosis, squamous cell carcinoma and fibroid. Though the occurrence of sarcoid and simultaneous habronemosis in horses is rare in equine medicine, clinicians and pathologists who work with diagnosis may sporadically encounter similar cases, hence the importance of histopathologic analysis of skin samples, as this may help definition of the a etiology and also the institution of therapeutic measures and prognosis of affected animals.Keywords: equine skin disease, skin neoplasms, parasitic infection

Hemorrhagic Septic Thrombophlebitis in Horses

 Background: Thrombophlebitis represents the main disease of the cardiovascular system of horses, its occurrence is strongly associated with the use of inappropriate materials and techniques. Its clinical presentation varies according to the degree of vessel obstruction and the appearance of complications, in the diagnosis it is essential to assess the extent of damage and the severity of the case. Establishing appropriate treatments, it should be noted that these are mostly clinical, reserving surgical interventions for severe cases, so the aim of the study is report a case of hemorrhagic septic thrombophlebitis treated by partial phlebectomy of the left jugular vein.Case: A 9-year-old male castrated equine, with no defined racial pattern, weighing 345 kg, used in the practice of vaquejada was attended at the Veterinary Hospital (HV) of the Federal University of Campina Grande (UFCG), Campus Patos (PB). During the anamnesis, it was reported that the animal presented colic a month ago, it was treated, recovered and since then, it started presenting an area with increase in volume in the neck region that ruptured the day before the HV attendance, where blood and pus came from. On physical exam, slight edema was noted in the left masseteric region, a volume rise involving the middle and cranial third of the neck with firm consistency in the left jugular sulcus, sensitivity to palpation and little drainage of purulent bloody secretion was observed. Additionally, tachycardia, tachypnea and intestinal hypomotility were found. In turn, in the ultrasound exam, a hypoechoic structure was seen, causing partial obstruction of the vessel, proximal to the fistulated region and total obstruction distal to it. With this information, antibiotic therapy was prescribed, a warm compress followed by the use of anti-inflammatory gel every 8 hours. By choice of the owner, the animal returned to the farm, in the next day returned to the Veterinary Hospital, due to extensive bleeding observed on the estate, immediately tried to stanch the bleeding by compressive banding, without success, we opted for the surgical intervention aiming to perform ligation and partial resection of the jugular vein. Pre-anesthetic medication was performed and under general anesthesia a rectilinear incision was made over the left jugular vein of the neck caudal region to bifurcation of lingual and facial veins, blunt dissection aiming to loosen the vessel and hemostasis of the installed neovascularization, transfixing proximal ligation with 1-0 nylon thread, diaeresis, removal of the vessel, reduction of the subcutaneous space, application of drain, dermorrhaphy in simple continuous pattern and use of compressive curative. For the postoperative period, maintenance of the initial antibiotic therapy was prescribed, adding flunixin meglumine 1.1 mg/kg, i.m, SID, 4 applications, tetanus serum 5000 UI/IM, antiphlogistic massage in the masseter region and wound treatment by washing with hypersaturated solution, use of sugar, healing pomade and repellent. One month after surgery, the animal received medical release with satisfactory healing, recovery from anemic and infectious condition, without circulatory complications.Discussion: The present report shows the feasibility of unilateral partial phlebectomy of the jugular vein as a therapeutic option in complicated cases of thrombophlebitis. In which thrombectomy techniques are contraindicated and ineffective clinical treatments, another alternative is vascular transplantation, which encounters many logistical difficulties in the routine. Despite the interruption of blood flow being pointed out as an aggravating factor, it should be noted that often thrombophlebitis itself leads to this condition, and the development of collateral circulation secondary to venous flow obstruction has been observed

Analno-vulvarna atrezija i ageneza kokcigealnih kralježaka u puleta - prikaz slučaja

Anal-vulvar atresia and agenesis of the coccygeal vertebrae is a rare condition that has never been reported before in donkeys, and only a single case in sheep. In this light, we sought to describe the surgical approach and postoperative care of a newborn donkey with this congenital abnormality. A female donkey was treated that was less than 24 hours old, weighing 15 kg, with increased perineal volume and the absence of an anus. The diagnosis was made by clinical examination, which also found an absence of the vulva and coccygeal vertebrae. A surgical approach followed using anoplasty and temporary fixation of a plastic probe, and a urethra attached to the skin. The postoperative period was followed by daily cleansing, antibiotic, anti-inflammatory and analgesic therapy, and removal of the plastic tube at 10 days. The early diagnosis and treatment enabled complete success and the animal’s recovery.Analno-vulvarna atrezija i ageneza kokcigealnih kralježaka rijetko je stanje koje dosad nije opisano u magaraca, naime opisano je samo u jednom slučaju u ovce. Upravo smo zbog toga nastojali opisati kirurški pristup i poslijeoperacijsku njegu u novorođenog puleta s ovom kongenitalnom abnormalnošću. Riječ je o ženki, staroj manje od 24 sata, tjelesne mase 15 kg, s povećanim perinealnim volumenom i bez anusa. Dijagnoza je postavljena na temelju kliničkog pregleda koji je otkrio i odsutnost vulve i kokcigealnih kralježaka. Uslijedio je kirurški zahvat s anoplastikom i privremenim postavljanjem plastične sonde i uretre pričvršćene za kožu. Poslijeoperacijski je period uključio dnevno čišćenje, antibiotičku, protuupalnu i analgetičku terapiju te uklanjanje plastične cijevi nakon deset dana. Rana dijagnoza i liječenje omogućili su potpun uspjeh u oporavku životinje

Correção cirúrgica como tratamento de meningocele associada à craniosquise em bezerro: relato de caso

O objetivo desse trabalho é descrever um caso de meningocele associada à craniosquise em um bezerro SRD, de três dias de idade, que nasceu com aumento de volume na porção frontal da cabeça, sem manifestação de alterações neurológicas ou sensibilidade na região. O exame físico associado ao radiográfico confirmaram a anomalia como sendo craniosquise associada a meningocele, que foi reparada cirurgicamente. O animal foi submetido ao protocolo anestésico com xilazina a 2% (0,05 mg/kg) via intramuscular, a indução e manutenção transcorreu com uso do anestésico geral isofluorano via inalatória e no bloqueio local utilizou-se lidocaína 2% sem vasoconstrictor. Foi drenado do aumento de volume aproximadamente 1,5 litros de líquor e realizada uma incisão elíptica contornando a bolsa, mantendo pele suficiente nas bordas para posterior sutura. O tamanho da falha na região frontal era de 7 cm de diâmetro e após drenagem foi realizada sutura das bordas das meninges em padrão simples contínuo e dermorrafia em padrão simples separado, utilizando fio nylon 0,35 e nylon 0,40 respectivamente. No pós-operatório foi utilizado antibioticoterapia, anti-inflamatórios, vitamina B1 e furosemida. A limpeza da ferida cirúrgica foi realizada diariamente com álcool iodado, spray cicatrizante e a retirada dos pontos ocorreu dez dias após o procedimento. Seguidos 39 dias o animal recebeu alta, apresentando-se saudável e sem deformidade ou recidivas do acúmulo de líquor. O tratamento cirúrgico foi eficaz e deve ser utilizado em casos semelhantes. Para redução do tempo quanto a recuperação dos pacientes, novas técnicas devem ser avaliadas, como a utilização de enxertos para correção da falha craniana em ruminantes

Mastitis by Pythium insidiosum in Mares

Background: Pythiosis is a chronic inflammatory disease caused by the oomycete Pythium insidiosum. This disease has been reported in several species, but most cases occur in horses. In this species, the cutaneous form is more prevalent, with occasional involvement of adjacent lymph nodes and bone tissue. The involvement of the mammary glands was not properly characterized. Therefore, the aim of this study is to describe cases of pythiosis with the involvement of the mammary glands in mares.Case: A retrospective study of all biopsy samples and necropsy examinations of horses, performed at the Animal Pathology Laboratory of the Federal University of Campina Grande from January 2000 to December 2018, was carried out. During the study period, 142 cases of cutaneous pythiosis were identified. Of these, in three the lesions extended to the mammary glands. Mares with 3-, 7- and 12-year-old, mongrel, from the backlands of the Paraiba were affected. On physical examination, the mares presented swollen mammary glands and multifocal wounds, which discharge serosanguinolent secretion. Clinical evolution ranged from 15 to 30 days. The animals had been medicated, but the wounds did not heal and mastectomies were performed. Macroscopically, the mammary glands were enlarged and with multifocal areas of ulceration measuring 1 to 3.5 cm in diameter. At the cut surface, they exhibited fistulous tracts containing kunkers. In the histopathological evaluation, was observed diffuse and marked inflammatory infiltrate of eosinophils extending through the deep dermis and interlobular mammary stroma; and multifocal areas, sometimes focally extensive, of eosinophilic necrosis with intralesional negative images of hyphae. In histological sections stained by periodic acid–Schiff, the walls of the hyphae were weakly stained in blue; but when impregnated by Grocott methenamine silver, they were strongly marked in black and were characterized by semi-parallel walls, rare septations and branching, and a diameter varying from 3-8μm. At immunohistochemistry, the hyphae showed strong cytoplasmic and wall immunostaining for the anti-P. insidiosum antibody.Discussion: The diagnosis of mastitis by P. insidiosum was established based on the epidemiological, clinical, anatomopathological and immunohistochemical findings. Lesions in horses are more frequently located at the extremities of the limbs and the ventral portion of the thoracoabdominal wall. In these cases, it is likely that the lesions have started on the skin adjacent to the udder and have extended to the mammary parenchyma. The intralesional structures known as kunkers have been described in horses, mules, donkeys, and camels, being absent in the other animal species affected. The kunkers are always present in equine pythiosis and allow the establishment of an accurate presumptive diagnosis. Clinically important findings include wounds that discharge serosanguineous secretions and do not heal through conventional treatments. Histological lesions characterized by areas of eosinophilic necrosis with intralesional hyphae, which are weakly stained by PAS and strongly impregnated by GMS, are highly suggestive of pythiosis. Pythiosis is relatively frequent in horses in the backlands of Paraiba, predominantly in the cutaneous form. The involvement of the mammary glands is an uncommon presentation that should be included as a differential diagnosis of mastitis in mares

Estudo do efeito da monensina sódica na prevenção da acidose láctica ruminal induzida em caprinos

Objetivou-se com este trabalho estudar o comportamento clínico e laboratorial de caprinos submetidos à incorporação da monensina sódica na alimentação e avaliar os seus efeitos na prevenção da acidose láctica ruminal induzida experimentalmente. Foram avaliados os aspectos clínicos como atitude, comportamento, apetite, coloração das mucosas externas, freqüência cardíaca e respiratória, motilidade retículo-ruminal, temperatura retal e o aspecto das fezes, e as características físico-químicas e microbiológicas do fluido ruminal. Foram utilizados 20 caprinos, machos, castrados, cruzados Anglo Nubiana x Saanen, com peso médio de 30Kg, clinicamente sadios e submetidos a implantação de cânulas ruminais permanentes. Foram formados dois grupos de 10 animais, um grupo controle (GC) e outro que recebeu a monensina sódica (GM) através da cânula, na dose diária de 33mg/Kg da dieta, por animal, no decorrer de 40 dias. A acidose láctica ruminal foi induzida fornecendo 10g de sacarose/Kg de peso corpóreo, antes da alimentação matinal. As observações clínicas e a colheita das amostras de fluido ruminal foram efetuadas em intervalos de 4h, 8h, 12h, 24h, 32h, 48h e 72h pós-indução (PI). A partir das 4 horas PI, evidenciou-se sinais como apatia, apetite caprichoso ou anorexia, taquicardia, taquipnéia, atonia ruminal, distensão abdominal e diarréia de intensidade variável. O refluxo de fluido ruminal pelas narinas, sinais de cólica intestinal e secreção nasal serosa bilateral foi observado em alguns animais do GC, e laminite no GM. Ocorreu perda média de peso corpóreo de 900g no GC (P>0,05) e de 1,3Kg no GM (P0,05). A cor do fluido ruminal tornou-se leitosa, o odor ácido e a consistência aquosa. Houve um aumento significativo (P0.05) and 1.3Kg in GM (P0.05). The color of the rumen fluid became milky, acid smell and watery consistency. There was a significant increase (P <0.05) the acidity, of the test time reduction of methylene blue (PRAM), the values of chloride and lactic acid. The dynamics of the fauna and flora has changed, with a predominance of Grampositive. In some animals there was no full restoration of all variables. The use of monensin did not prevent the onset of the disorder fermentation in animals that received.Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqCoordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPE