School Food Environments and Policies in U.S. Public Schools

Examines food environments in elementary, middle, and high schools based on seventeen factors, including foods and beverages offered, the availability of vending machines, and how they vary by grade level, location, and other school characteristics

Does the Type of Legal Representation Affect Sentencing Outcomes?

This paper examines the potential differences in sentencing outcomes for public defenders compared with private attorneys. I explore the economic literature for explanations, implications, and results. There is a need for extensive research within the field of economics to provide empirical results to offset the mostly game-theoretically dominated discussion of the criminal justice system. The results are inconclusive due to a lack of statistical significance and potential selection bias in the data. Future research relating to this study is also discussed

Is acupuncture a useful adjunct to physiotherapy for older adults with knee pain?: The "Acupuncture, Physiotherapy and Exercise" (APEX) study [ISRCTN88597683]

BACKGROUND: Acupuncture is a popular non-pharmacological modality for treating musculoskeletal pain. Physiotherapists are one of the largest groups of acupuncture providers within the NHS, and they commonly use it alongside advice and exercise. Conclusive evidence of acupuncture's clinical effectiveness and its superiority over sham interventions is lacking. The Arthritis Research Campaign (arc) has funded this randomised sham-controlled trial which addresses three important questions. Firstly, we will determine the additional benefit of true acupuncture when used by physiotherapists alongside advice and exercise for older people presenting to primary care with knee pain. Secondly, we will evaluate sham acupuncture in the same way. Thirdly, we will investigate the treatment preferences and expectations of both the participants and physiotherapists participating in the study, and explore the effect of these on clinical outcome. We will thus investigate whether acupuncture is a useful adjunct to advice and exercise for treating knee pain and gain insight into whether this effect is due to specific needling properties. METHODS/DESIGN: This randomised clinical trial will recruit 350 participants with knee pain to three intervention arms. It is based in 43 community physiotherapy departments in 21 NHS Trusts in the West Midlands and Cheshire regions in England. Patients aged 50 years and over with knee pain will be recruited. Outcome data will be collected by self-complete questionnaires before randomisation, and 6 weeks, 6 months and 12 months after randomisation and by telephone interview 2 weeks after treatment commences. The questionnaires collect demographic details as well as information on knee-related pain, movement and function, pain intensity and affect, main functional problem, illness perceptions, self-efficacy, treatment preference and expectations, general health and quality of life. Participants are randomised to receive a package of advice and exercise; or this package plus real acupuncture; or this package plus sham acupuncture. Treatment details are being collected on a standard proforma. Interventions are delivered by experienced physiotherapists who have all received training in acupuncture to recognised national standards. The primary analysis will investigate the main treatment effects of real or sham acupuncture as an adjunct to advice and exercise. DISCUSSION: This paper presents detail on the rationale, design, methods, and operational aspects of the trial

The Relationship Between Menopause and Marital Satisfaction in Adult Women

The purpose of this non-experimental study was to determine if menopause has an effect on marital satisfaction, depression, and weight gain in women. Participants were a convenience sample of 100 volunteers. The participants completed a survey to determine the effects of menopause on marital satisfaction, depression, and weight gain. An analysis of the results revealed that menopause does not have a significant impact on marital satisfaction or weight gain. The results did reveal a significant connection between menopause and depression

Children’s Gender Development: Sibling Configuration and Maternal Influence

We present a study of 3- to 8-year-old children in various family types. Family type influenced some outcomes, although many measures were unrelated to family type. Additionally, some relationships between maternal attitudes about gender-related behavior differed between boys and girls, and in different family configurations

Shale Gas Development and Infant Health: Evidence from Pennsylvania

WP 2012-12 December 201

The Economic and Health Effects of the Chemical Spill in the Elk River

In January 2014, Freedom Industries spilled 4-methylcyclohexylmethanol, a chemical foaming agent used in coal processing, from a storage facility into the Elk River in West Virginia. This chemical spill, one of the most significant in U.S. history, adversely affected the drinking water supply of over 300,000 individuals in the Charleston, West Virginia Metropolitan area. We use synthetic control methods to estimate the casual effects on macro-economic growth and infant health outcomes from this water crisis. We find a significant decrease in 5-minute Apgar Scores, a measure of how babies fare in the birthing process and outside of the womb, after the chemical spill. We do not find significant effects for infant birthweight or gestational age. We find a statistically insignificant decrease of per capita GDP in the Charlestown, West Virginia area compared to the synthetic control of 3% two years after the chemical spill

Listeriolysin S, a Novel Peptide Haemolysin Associated with a Subset of Lineage I Listeria monocytogenes

peer-reviewedStreptolysin S (SLS) is a bacteriocin-like haemolytic and cytotoxic virulence factor that plays a key role in the virulence of Group A Streptococcus (GAS), the causative agent of pharyngitis, impetigo, necrotizing fasciitis and streptococcal toxic shock syndrome. Although it has long been thought that SLS and related peptides are produced by GAS and related streptococci only, there is evidence to suggest that a number of the most notorious Gram-positive pathogenic bacteria, including Listeria monocytogenes, Clostridium botulinum and Staphylococcus aureus, produce related peptides. The distribution of the L. monocytogenes cluster is particularly noteworthy in that it is found exclusively among a subset of lineage I strains; i.e., those responsible for the majority of outbreaks of listeriosis. Expression of these genes results in the production of a haemolytic and cytotoxic factor, designated Listeriolysin S, which contributes to virulence of the pathogen as assessed by murine- and human polymorphonuclear neutrophil–based studies. Thus, in the process of establishing the existence of an extended family of SLS-like modified virulence peptides (MVPs), the genetic basis for the enhanced virulence of a proportion of lineage I L. monocytogenes may have been revealed.Work is funded by the Irish Government under the National Development Plan, through a Science Foundation Ireland Investigator award to CH, PR and PC (06/IN.1/B98)

Breast cancer cell migration is regulated through junctional adhesion molecule-A-mediated activation of Rap1 GTPase

ABSTRACT: INTRODUCTION: The adhesion protein junctional adhesion molecule-A (JAM-A) regulates epithelial cell morphology and migration, and its over-expression has recently been linked with increased risk of metastasis in breast cancer patients. As cell migration is an early requirement for tumor metastasis, we sought to identify the JAM-A signalling events regulating migration in breast cancer cells. METHODS: MCF7 breast cancer cells (which express high endogenous levels of JAM-A) and primary cultures from breast cancer patients were used for this study. JAM-A was knocked down in MCF7 cells using siRNA to determine the consequences for cell adhesion, cell migration and the protein expression of various integrin subunits. As we had previously demonstrated a link between the expression of JAM-A and β1-integrin, we examined activation of the β1-integrin regulator Rap1 GTPase in response to JAM-A knockdown or functional antagonism. To test whether JAM-A, Rap1 and β1-integrin lie in a linear pathway, we tested functional inhibitors of all three proteins separately or together in migration assays. Finally we performed immunoprecipitations in MCF7 cells and primary breast cells to determine the binding partners connecting JAM-A to Rap1 activation. RESULTS: JAM-A knockdown in MCF7 breast cancer cells reduced adhesion to, and migration through, the β1-integrin substrate fibronectin. This was accompanied by reduced protein expression of β1-integrin and its binding partners αV- and α5-integrin. Rap1 activity was reduced in response to JAM-A knockdown or inhibition, and pharmacological inhibition of Rap1 reduced MCF7 cell migration. No additive anti-migratory effect was observed in response to simultaneous inhibition of JAM-A, Rap1 and β1-integrin, suggesting that they lie in a linear migratory pathway. Finally, in an attempt to elucidate the binding partners putatively linking JAM-A to Rap1 activation, we have demonstrated the formation of a complex between JAM-A, AF-6 and the Rap1 activator PDZ-GEF2 in MCF7 cells and in primary cultures from breast cancer patients. CONCLUSIONS: Our findings provide compelling evidence of a novel role for JAM-A in driving breast cancer cell migration via activation of Rap1 GTPase and β1-integrin. We speculate that JAM-A over-expression in some breast cancer patients may represent a novel therapeutic target to reduce the likelihood of metastasis