Effect of Different Storage Temperatures on the Efficacy of the Bivalent Foot and Mouth Disease Oil Vaccine

The storage stability of locally produced double oil emulsion adjuvant bivalent Foot and mouth disease (FMD) vaccine prepared from type O1/Aga/ EGY/93 strain and A/EGY/1/2006 had been determined depending on its shelf life in different storage temperatures during the registration of this vaccine by the Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo. Samples of this vaccine were kept at 4°C for period of 27 months; at 25°C for 5 weeks and at 37°C for 3 weeks. The potency of these vaccine samples was evaluated in guinea pigs as laboratory animal's model. The obtained results confirmed that the vaccine keep its potency beyond the normal conservation period at 4°C for two years with 100% protection against challenge with FMDV O1/Aga/EGY/93 and at 25°C for 3 weeks and at 37°C for 1 week, showing 80% protection when storage of the vaccine at 25°C for 4 weeks; at 37°C for 2 weeks. On challenge with A/EGY/1/2006 the vaccine gave 100% protection when storage at 4°C for 21 months; at 25°C for 2 weeks and at 37°C for 1 week. Otherwise it gave 80% protection when storage at 4°C for 24 months; at 25°C for 3 weeks and at 37°C for 2 weeks then became invalid after 27 months at 4°C; after 4 weeks at 25°C and for 3 weeks at 37°C. So it could be concluded that 4°C is the best temperature of choice for storage of the oil inactivated bivalent FMD vaccine

Single-atom entropy squeezing for two two-level atoms interacting with a single-mode radiation field

In this paper we consider a system of two two-level atoms interacting with a single-mode quantized electromagnetic field in a lossless resonant cavity via $l$-photon-transition mechanism. The field and the atoms are initially prepared in the coherent state and the excited atomic states, respectively. For this system we investigate the entropy squeezing, the atomic variances, the von Neumann entropy and the atomic inversions for the single-atom case. We show that the more the number of the parties in the system the less the amounts of the nonclassical effects exhibited in the entropy squeezing. The entropy squeezing can give information on the corresponding von Neumann entropy. Also the nonclassical effects obtained form the asymmetric atoms are greater than those obtained form the symmetric atoms. Finally, the entropy squeezing gives better information than the atomic variances only for the asymmetric atoms.Comment: 15 pages, 4 figures, comments are most welcom

Interactive effects of soil salinity and water table depth on soil properties and sorghum (Sorghum bicolor L. Moench) production

Soil salinity and water table are the most prevalent and widespread problems limiting crop productivity in irrigated agriculture. Our experiment aims to evaluate the effect of soil salinity and water table on some soil properties, growth, and yield of sorghum (variety Giza 15) grown along two successive seasons. Nine different sites were chosen, representing three levels of soil salinity (i.e. ECe < 4, 4-8 and 8-16 dS m-1). For each salinity level three water table were selected (i.e. shallow ≈ 55 cm, medium ≈ 80 cm and deep ≈ 120 cm). Results revealed that there are main considerable effects of salinity and water table on some soil properties. Increasing of salinity caused significant decreases in plant growth, weight of 1000 grains (g), protein content% and  seed yield (t/ha) which decreased by 36.98%, 32.27%, 20.45%, 29.95 % and 57.46% respectively, when salinity increased from S1  to S3. On the other hand, decreasing of water table lead to improvement in all mentioned soil properties and plant growth. The results indicated that need to maintain low or moderate salinity and deep water table, which is essential for producing high sorghum grain yield with satisfactory quality

QUERCETIN MITIGATES TOXICITY AND OXIDATIVE STRESS MOTIVATED BY BISPHENOL A IN LIVER OF MALE RATS

Objective: Epidemiological reports have indicated a correlation between the increasing of bisphenol A (BPA) levels in the environment and the incidence of hepatotoxicity. The present study aimed to evaluate the protective effect of quercetin on oxidative stress, inflammatory markers, apoptotic and antiapoptotic markers in the liver tissue of the bisphenol A treated rats.Methods: Forty-eight male Wistar rats were divided into six groups; Group(1): Negative control group (Con), Group(2): Corn oil control group orally administered 1 ml of corn oil/rat daily for two months (Corn), Group(3): Olive oil control group orally administered 1 ml olive oil/rat daily for two months (Olive), Group(4): Quercetin (Qu) control group orally received Qu dissolved in olive oil (50 mg/kg b. wt.) daily for two months (Qu). Group(5): Positive control group orally received Bisphenol A (BPA) dissolved in corn oil in a dose of 50 mg/kg b. wt. daily for two months (BPA), Group(6): Quercetin treated group orally administered 50 mg/kg b. wt. of BPA and treated with Qu (50 mg/kg b. wt. Orally) daily for two months (BPA+Qu).Results: BPA exposure resulted in significant elevations of oxidative stress, as evidenced by the increased malondialdehyde level and glutathione-S-transferase activity associated with significant decrease in glutathione peroxidase activity in the liver tissue. Moreover, BPA caused an up regulation in the values of liver function enzymes. Also, BPA produced a significant elevation in the hepatic Interleuckin-6 (IL-6) and caspase-3 levels with a significant decline in antiapoptotic protein B-cell lymphoma 2 (Bcl2) level in liver tissue. Quercetin significantly attenuated the BPA-evoked liver oxidative stress and modulated the activities of liver function enzymes. In addition, treatment of quercetin with BPA resulted in an improvement of IL-6 and caspase-3 levels associated with a significant increase in hepatic protein Bcl2 expression.Conclusion: These data suggest that quercetin protects rat liver from BPA-induced oxidative stress, probably via its antioxidant activity, anti-inflammatory and antiapoptotic effects. So, Quercetin is a promising pharmacological agent for preventing the potential hepatotoxicity of BPA following occupational or environmental exposures.Keywords: Bisphenol A, Quercetin, hepatotoxicity, Antioxidant, Anti-inflammatory effect, Antiapoptotic effec

Metabolic Syndrome and Its Components : Secondary analysis of the World Health Survey, Oman

Objectives: The study aimed to describe the prevalence of metabolic syndrome (MS) and its components among Omani adults. Methods: The 2008 Oman World Health Survey dataset was used to determine the national prevalence of MS. Logistic regression using all key sociodemographic, clinical and behavioural variables was used to identify the associations of independent variables with MS. Results: The age-adjusted prevalence of MS was 23.6%. MS was significantly associated with age, marital and work status and wealth level. MS was more common for people aged 50 years and older compared to the youngest cohort (OR 3.6, CI: 2.4–5.3; P <0.001) and in people who were married or employed (OR 1.6, CI: 1.3–2.1; P <0.001 and OR 1.3, CI: 1.1–1.8; P = 0.043, respectively) compared to their unmarried and unemployed counterparts. MS was also more common in people in the second lowest wealth quintile (OR 1.6, CI: 1.2–2.2; P = 0.05) compared to the lowest quintile and in those who sat for more than six hours per day (OR 1.3, CI: 1.1-1.7; P = 0.035). Conclusion: One in four adults had MS in Oman. This may fuel the epidemic of non-communicable diseases (NCDs) in Oman, particularly given the increasingly elderly population. Urgent action is required to ensure quality patient care at all levels of the healthcare system. Further research on behavioural risk factors is needed. Developing and implementing a multisectoral strategy to prevent NCDs should be at the top of the current health agenda for Oman

Effect of Sodium Lauryl Sulfate-Fumaric Acid Coupled Addition on the In Vitro

The aim of the current study was to evaluate the effect of sodium lauryl sulfate-fumaric acid coupled addition on in vitro methangenesis and rumen fermentation. Evaluation was carried out using in vitro gas production technique. Ruminal contents were collected from five steers immediately after slaughtering and used for preparation of inoculums of mixed rumen microorganisms. Rumen fluid was then mixed with the basal diet of steers and used to generate four treatments, negative control (no additives), sodium lauryl sulfate (SLS) treated, fumaric acid treated, and SLS-fumaric acid coupled addition treated. The results revealed that, relative to control, efficiency in reduction of methanogenesis was as follows: coupled addition > SLS-addition > fumaric acid addition. Both SLS-addition and SLS-fumaric acid coupled addition demonstrated a decremental effect on ammonia nitrogen (NH(3)–N), total short chain volatile fatty acids (SCVFAs) concentrations and the amount of substrate degraded, and an increment effect on microbial mass and microbial yield (Y(ATP)). Nevertheless, fumaric acid did not alter any of the previously mentioned parameters but induced a decremental effect on NH(3)–N. Furthermore, both fumaric acid and SLS-fumaric acid coupled addition increased propionate at the expense of acetate and butyrate, while, defaunation increased acetate at the expense of propionate and butyrate. The pH value was decreased by all treatments relative to control, while, cellulase activity did not differ by different treatments. The current study can be promising strategies for suppressing ruminal methane emissions and improving ruminants feed efficiency

Comparative Study between the Performances of Nile Tilapia Oreochromisniloticus during and Out of the Normal Spawning Season

During the production season (2010-2011), this work was carried out at a commercial tilapia hatchery in Motobas, Kafr El-Sheikh Governorate- Egypt. Two experiments were managed using the same design to make a comparison between spawning of Nile tilapia Oreochromisniloticusbroodstock off-season (the winter) and on-season (the summer). The two experiments were tested by studying the effects of using feed additive (Nuvisol hatch P® 0.1%), different broodstocksizes (350, 200, 150 and mixed up to 250 g/fish) and stocking densities (50, 55, 60 female/pond-24m2) on growth performance, feed utilization, reproductive performance and economical profitability parameters of Nile tilapia, O.niloticus spawned in the summer and in the winter. Comparing the results of the economic analysis of the two experiments showed that the total production of Nile tilapia fry per each spawning pond, 24 square meters, is 28,090 within the natural spawning season, an increase of 2.23% from that was spawning outside the normal season (27478 fry). Though total revenue and net income under hatchery conditions in the out off-season (February 2010) much higher than that in natural spawning season (April 2011) by 22.01%. This is of course due to the price of tilapia fry in the winter months is higher than the summer to supply shortages in winter and increased demand at the same time. This is due to the farmers need to start the growing season early, March/April, in order to harvest their fish before temperatures drop in the next winter, which adversely affect the life of the fish

Development of an enhanced analytical method utilizing pepper matrix as an analyte protectant for sensitive GC-MS/MS detection of dimethipin in animal-based food products

Herein, an analytical method using gas chromatography-tandem mass spectrometry (GC- MS/MS) was devised to detect the presence of the troublesome pesticide dimethipin in various animal-based food products, including chicken, pork, beef, eggs, and milk. The injection port was primed with a matrix derived from pepper leaves that acts as an analyte protectant (AP) to safeguard the target compound from thermal degradation during gas chromatography. The presence of AP resulted in a remarkable limit of quantification of 0.005 mg/kg for dimethipin in five matrices. Three different versions (original, EN, and AOAC) of the QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) method were compared for dimethipin extraction, with a double-layer solid-phase extraction (SPE) cartridge utilized for matrix purification. A seven-point external calibration curve was established for dimethipin in the five matrices, demonstrating excellent linearity with determination coefficients (R2) ≥ 0.998. The developed quantitative method was validated by fortifying each matrix with three different concentrations of standard dimethipin, and the average recovery fell within the acceptable range outlined in the CODEX guidelines (ranging from 88.8% to 110.0%), with a relative standard deviation (RSD) of ≤ 11.97%. This method effectively addresses the challenge of analyzing dimethipin and can therefore be used as a routine monitoring tool for dimethipin across various matrices

The combining ability for grain yield and some related characteristics in rice (Oryza sativa L.) under normal and water stress conditions

Drought is considered a major threat to rice production. This study aimed to determine the effects of drought stress on the estimates of heterosis and the combining ability of rice genotypes for the number of days to 50% heading, plant height, number of panicles per plant, panicle length, number of filled grains per panicle, and grain yield per plant. Field experiments were conducted at the Rice Research and Training Center, Kafr El Sheikh, Egypt, during the rice-growing season in 2018 and 2019. Eight rice genotypes (Giza178, Giza179, Sakha106, Sakha107, Sakha108, WAB1573, NERICA4, and IET1444) were crossed in a half-diallel cross in the rice-growing season in 2018, which yielded a wide range of variability in numerous agronomic traits and drought tolerance measurements. In 2019, these parents and their 28 F1 crosses were produced by employing a three-replication randomized complete block design under normal and water stress conditions. The results showed remarkable differences across the studied genotypes under normal and water stress conditions. Under both conditions, Sakha107 was the best general combiner for earliness and short stature. Giza179 and Sakha108 were the best general combiners for grain yield per plant and one or more of its characteristics. Furthermore, in both normal and water stress conditions, Giza179 exhibited the highest general combining ability effects for all attributes that were evaluated. Under normal and water stress conditions, the Giza179 × Sakha107 cross demonstrated substantial and desirable specific combining ability effects on all the examined traits, which suggested that it could be considered for use in rice hybrid breeding programs. Therefore, we recommend that these vital indirect selection criteria to be considered for improving rice grain yield under drought conditions