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Erforschung plasmachemischer Prozesse zur Polymerisation kolloidaler Systeme, Teilvorhaben Untersuchungen von plasmatechnischen Verfahren zur Polymerisation von micellaren Aggregaten ; Abschlussbericht
[no abstract available
Simulation of transient energy distributions in sub-ns streamer formation
Breakdown and streamer formation is simulated in atmospheric pressure nitrogen for a 2D planar electrode system. A PIC code with multigrid potential solver is used to simulate the evolution of the non-equilibrium ionization front on sub-nanosecond timescales. The ion and electron energy distributions are computed, accounting for the inclusion of inelastic scattering of electrons, and collisionally excited metastable production and ionization. Of particular interest is the increased production of metastable and low-energy ions and electrons when the applied field is reversed during the progress of the ionization front, giving insight into the improved species yields in nanosecond pulsed systems
Separation of VUV/UV photons and reactive particles in the effluent of a He/O2 atmospheric pressure plasma jet
Cold atmospheric pressure plasmas can be used for treatment of living tissues
or for inactivation of bacteria or biological macromolecules. The treatment is
usually characterized by a combined effect of UV and VUV radiation, reactive
species, and ions. This combination is usually beneficial for the effectiveness
of the treatment but it makes the study of fundamental interaction mechanisms
very difficult. Here we report on an effective separation of VUV/UV photons and
heavy reactive species in the effluent of a micro scale atmospheric pressure
plasma jet (-APPJ). The separation is realized by an additional flow of
helium gas under well-defined flow conditions, which deflects heavy particles
in the effluent without affecting the VUV and UV photons. Both components of
the effluent, the photons and the reactive species, can be used separately or
in combination for sample treatment. The results of treatment of a model plasma
polymer film and vegetative Bacillus subtilis and Escherichia coli cells are
shown and discussed. A simple model of the He gas flow and reaction kinetics of
oxygen atoms in the gas phase and at the surface is used to provide a better
understanding of the processes in the plasma effluent. The new jet
modification, called X-Jet for its appearance, will simplify the investigation
of interaction mechanisms of atmospheric pressure plasmas with biological
samples.Comment: 10 pages, 7 figures, submitted to Journal of Physics D: Applied
Physic
Concepts and characteristics of the 'COST Reference Microplasma Jet'
Biomedical applications of non-equilibrium atmospheric pressure plasmas have attracted intense interest in the past few years. Many plasma sources of diverse design have been proposed for these applications, but the relationship between source characteristics and application performance is not well-understood, and indeed many sources are poorly characterized. This circumstance is an impediment to progress in application development. A reference source with well-understood and highly reproducible characteristics may be an important tool in this context. Researchers around the world should be able to compare the characteristics of their own sources and also their results with this device. In this paper, we describe such a reference source, developed from the simple and robust micro-scaled atmospheric pressure plasma jet (ÎŒ-APPJ) concept. This development occurred under the auspices of COST Action MP1101 'Biomedical Applications of Atmospheric Pressure Plasmas'. Gas contamination and power measurement are shown to be major causes of irreproducible results in earlier source designs. These problems are resolved in the reference source by refinement of the mechanical and electrical design and by specifying an operating protocol. These measures are shown to be absolutely necessary for reproducible operation. They include the integration of current and voltage probes into the jet. The usual combination of matching unit and power supply is replaced by an integrated LC power coupling circuit and a 5âW single frequency generator. The design specification and operating protocol for the reference source are being made freely available
Gas mixing enhanced by power modulations in atmospheric pressure microwave plasma jet
Microwave plasma jet operating in atmospheric pressure argon was power modulated by audio frequency sine envelope in the 10^2 W power range. Its effluent was imaged using interference filters and ICCD camera for several different phases of the modulating signal. The combination of this fast imaging with spatially resolved optical emission spectroscopy provides useful insights into the plasmachemical processes involved. Phase-resolved schlieren photography was performed to visualize the gas dynamics. The results show that for higher modulation frequencies the plasma chemistry is strongly influenced by formation of transient flow perturbation resembling a vortex during each period. The perturbation formation and speed are strongly influenced by the frequency and power variations while they depend only weakly on the working gas flow rate. From application point of view, the perturbation presence significantly broadened lateral distribution of active species, effectively increasing cross-sectional area suitable for applications
Disinfection of Ocular Cells and Tissues by Atmospheric-Pressure Cold Plasma
Background: Low temperature plasmas have been proposed in medicine as agents for tissue disinfection and have received increasing attention due to the frequency of bacterial resistance to antibiotics. This study explored whether atmospheric-pressure cold plasma (APCP) generated by a new portable device that ionizes a flow of helium gas can inactivate ocular pathogens without causing significant tissue damage.
Methodology and Principal Findings: We tested the APCP effects on cultured Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Candida albicans, Aspergillus fumigatus and Herpes simplex virus-1, ocular cells (conjunctival fibroblasts and keratocytes) and ex-vivo corneas. Exposure to APCP for 0.5 to 5 minutes significantly reduced microbial viability (colony-forming units) but not human cell viability (MTT assay, FACS and Tunel analysis) or the number of HSV-1 plaque-forming units. Increased levels of intracellular reactive oxygen species (ROS) in exposed microorganisms and cells were found using a FACS-activated 2',7'-dichlorofluorescein diacetate probe. Immunoassays demonstrated no induction of thymine dimers in cell cultures and corneal tissues. A transient increased expression of 8-OHdG, genes and proteins related to oxidative stress (OGG1, GPX, NFE2L2) was determined in ocular cells and corneas by HPLC, qRT-PCR and Western blot analysis.
Conclusions: A short application of APCP appears to be an efficient and rapid ocular disinfectant for bacteria and fungi without significant damage on ocular cells and tissues, although the treatment of conjunctival fibroblasts and keratocytes caused a time-restricted generation of intracellular ROS and oxidative stress-related responses
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