Wild Bee Conservation within Urban Gardens and Nurseries: Effects of Local and Landscape Management

Across urban environments, vegetated habitats provide refuge for biodiversity. Gardens (designed for food crop production) and nurseries (designed for ornamental plant production) are both urban agricultural habitats characterized by high plant species richness but may vary in their ability to support wild pollinators, particularly bees. In gardens, pollinators are valued for crop production. In nurseries, ornamental plants rarely require pollination; thus, the potential of nurseries to support pollinators has not been examined. We asked how these habitats vary in their ability to support wild bees, and what habitat features relate to this variability. In 19 gardens and 11 nurseries in California, USA, we compared how local habitat and landscape features affected wild bee species abundance and richness. To assess local features, we estimated floral richness and measured ground cover as proxies for food and nesting resources, respectively. To assess landscape features, we measured impervious land cover surrounding each site. Our analyses showed that differences in floral richness, local habitat size, and the amount of urban land cover impacted garden wild bee species richness. In nurseries, floral richness and the proportion of native plant species impacted wild bee abundance and richness. We suggest management guidelines for supporting wild pollinators in both habitats.DFG, 414044773, Open Access Publizieren 2019 - 2020 / Technische Universität Berli

Rapid increase in simulated North Atlantic dust deposition due to fast change of northwest African landscape during the Holocene

Marine sediment records from a series of core sites along the northwest African margin show a sudden increase in North Atlantic dust deposition at about 5&thinsp;ka&thinsp;BP that has been associated with an abrupt end of the African Humid Period (AHP). To assess the causes of the abrupt shift in North Atlantic dust deposition, we explore changes in the Holocene dust cycle and in North African climate and landscape by performing several time slice simulations from 8&thinsp;ka&thinsp;BP until the preindustrial era. To do this, we use the coupled aerosol–climate model ECHAM6–HAM2 including dynamic vegetation and interactive dust, wherein ocean conditions and lake surface area are prescribed for each time slice.We find a rapid increase in simulated dust deposition between 6 and 4&thinsp;ka&thinsp;BP that is fairly consistent with the abrupt change in marine sediment records at around 20°&thinsp;N close to the northwest African margin. At more northern and more remote cores, a significant change in dust deposition is noticeable roughly between 6 and 2&thinsp;ka&thinsp;BP in the simulations as well as in the records, but the change is less sharp compared to the near-margin core sites. The rapid change in simulated dust deposition is caused by a rapid increase in simulated dust emissions in the western Sahara, where the main dust sources for dust transport towards the North Atlantic are located. The sudden increase in dust emissions in the western Sahara is according to our simulations a consequence of a fast decline of vegetation cover from 22 to 18°&thinsp;N that might occur due to vegetation–climate feedbacks or due to the existence of a precipitation threshold on vegetation growth. Additionally, the prescribed gradual reduction of lake area enforces accelerated dust release as highly productive dust sources are uncovered. Concurrently with the continental drying, surface winds in the western Sahara are accelerated. Changes in the Saharan landscape and dust emissions south of 18°&thinsp;N and in the eastern Sahara play a minor role in driving the dynamics of North Atlantic dust deposition at the core sites. Our study identifies spatial and temporal heterogeneity in the transition of the North African landscape. As a consequence, implications from local data records on large-scale climate have to be treated with caution.</p

The new BELUGA setup for collocated turbulence and radiation measurements using a tethered balloon: First applications in the cloudy Arctic boundary layer

The new BELUGA (Balloon-bornE moduLar Utility for profilinG the lower Atmosphere) tethered balloon system is introduced. It combines a set of instruments to measure turbulent and radiative parameters and energy fluxes. BELUGA enables collocated measurements either at a constant altitude or as vertical profiles up to 1.5km in height. In particular, the instrument payload of BELUGA comprises three modular instrument packages for high-resolution meteorological, wind vector and broadband radiation measurements. Collocated data acquisition allows for estimates of the driving parameters in the energy balance at various heights. Heating rates and net irradiances can be related to turbulent fluxes and local turbulence parameters such as dissipation rates. In this paper the technical setup, the instrument performance, and the measurement strategy of BELUGA are explained. Furthermore, the high vertical resolution due to the slow ascent speed is highlighted as a major advantage of tethered balloon-borne observations. Three illustrative case studies of the first application of BELUGA in the Arctic atmospheric boundary layer are presented. As a first example, measurements of a single-layer stratocumulus are discussed. They show a pronounced cloud top radiative cooling of up to 6K h-1. To put this into context, a second case elaborates respective measurements with BELUGA in a cloudless situation. In a third example, a multilayer stratocumulus was probed, revealing reduced turbulence and negligible cloud top radiative cooling for the lower cloud layer. In all three cases the net radiative fluxes are much higher than turbulent fluxes. Altogether, BELUGA has proven its robust performance in cloudy conditions of the Arctic atmospheric boundary layer

Tree insect pests and pathogens: a global systematic review of their impacts in urban areas

Trees contribute greatly to urban environments and human well-being, yet relatively little is known about the extent to which a rising incidence of tree insect pests and pathogens may be affecting these contributions. To address this issue, we undertook a systematic review and synthesis of the diverse global empirical evidence on the impacts of urban tree insect pests and pathogens, using bibliographic databases. Following screening and appraisal of over 3000 articles from a wide range of fields, 100 studies from 28 countries, spanning 1979–2021, were conceptually sorted into a three-part framework: (1) environmental impacts, representing 95 of the studies, including those reporting on tree damage, mortality, reduced growth, and changes in tree function; (2) social impacts were reported by 35 of studies, including on aesthetics, human health, and safety hazards; and (3) economic impacts, reported in 24 of studies, including on costs of pest management, and economic losses. There has been a considerable increase in urban impact studies since 2011. Evidence gaps exist on impacts on climate-regulating capacity, including temperature regulation, water retention, soil erosion, and wind protection, but also on specific hazards, nuisances, human well-being, property damages, and hazard liabilities. As a knowledge synthesis, this article presents the best available evidence of urban tree insect / pathogen impacts to guide policy, management and further research. It will enable us to better forecast how growing threats will affect the urban forest and plan for these eventualities

Inositol Hexakisphosphate-Induced Autoprocessing of Large Bacterial Protein Toxins

Large bacterial protein toxins autotranslocate functional effector domains to the eukaryotic cell cytosol, resulting in alterations to cellular functions that ultimately benefit the infecting pathogen. Among these toxins, the clostridial glucosylating toxins (CGTs) produced by Gram-positive bacteria and the multifunctional-autoprocessing RTX (MARTX) toxins of Gram-negative bacteria have distinct mechanisms for effector translocation, but a shared mechanism of post-translocation autoprocessing that releases these functional domains from the large holotoxins. These toxins carry an embedded cysteine protease domain (CPD) that is activated for autoprocessing by binding inositol hexakisphosphate (InsP6), a molecule found exclusively in eukaryotic cells. Thus, InsP6-induced autoprocessing represents a unique mechanism for toxin effector delivery specifically within the target cell. This review summarizes recent studies of the structural and molecular events for activation of autoprocessing for both CGT and MARTX toxins, demonstrating both similar and potentially distinct aspects of autoprocessing among the toxins that utilize this method of activation and effector delivery

Impaired proteoglycan glycosylation, elevated TGF-β signaling, and abnormal osteoblast differentiation as the basis for bone fragility in a mouse model for gerodermia osteodysplastica

<div><p>Gerodermia osteodysplastica (GO) is characterized by skin laxity and early-onset osteoporosis. <i>GORAB</i>, the responsible disease gene, encodes a small Golgi protein of poorly characterized function. To circumvent neonatal lethality of the <i>Gorab</i>^<i>Null</i> full knockout, <i>Gorab</i> was conditionally inactivated in mesenchymal progenitor cells (Prx1-cre), pre-osteoblasts (Runx2-cre), and late osteoblasts/osteocytes (Dmp1-cre), respectively. While in all three lines a reduction in trabecular bone density was evident, only <i>Gorab</i>^Prx1 and <i>Gorab</i>^Runx2 mutants showed dramatically thinned, porous cortical bone and spontaneous fractures. Collagen fibrils in the skin of <i>Gorab</i>^<i>Null</i> mutants and in bone of <i>Gorab</i>^Prx1 mutants were disorganized, which was also seen in a bone biopsy from a GO patient. Measurement of glycosaminoglycan contents revealed a reduction of dermatan sulfate levels in skin and cartilage from <i>Gorab</i>^<i>Null</i> mutants. In bone from <i>Gorab</i>^Prx1 mutants total glycosaminoglycan levels and the relative percentage of dermatan sulfate were both strongly diminished. Accordingly, the proteoglycans biglycan and decorin showed reduced glycanation. Also in cultured <i>GORAB</i>-deficient fibroblasts reduced decorin glycanation was evident. The Golgi compartment of these cells showed an accumulation of decorin, but reduced signals for dermatan sulfate. Moreover, we found elevated activation of TGF-β in <i>Gorab</i>^Prx1 bone tissue leading to enhanced downstream signalling, which was reproduced in <i>GORAB</i>-deficient fibroblasts. Our data suggest that the loss of <i>Gorab</i> primarily perturbs pre-osteoblasts. GO may be regarded as a congenital disorder of glycosylation affecting proteoglycan synthesis due to delayed transport and impaired posttranslational modification in the Golgi compartment.</p></div